44 research outputs found
Breastfeeding prevalence in newborns of mothers with COVID-19: a systematic review
ABSTRACT Objectives: to compare exclusive breastfeeding prevalence versus artificial feeding in newborns of mothers with COVID-19. Methods: a systematic review of prevalence, according to JBI. Searches in PubMedÂź, Embase, CINAHL, LILACS and Web of Scienceâą databases in August 2021. Cross-sectional, longitudinal or cohort studies were selected, without language and time limitations that showed breastfeeding prevalence or that allowed calculation. Results: fifteen articles published in 2020 and 2021, cohort (60%) or cross-sectional (40%) were analyzed. The average of exclusive breastfeeding in mothers with COVID-19 was 56.76% (CI=39.90â72.88), and artificial breastfeeding, 43.23% (CI = 30.99 â 55.88), without statistically significant differences. Conclusions: despite the recommendations for maintaining breastfeeding, there was a reduction worldwide, when compared to periods prior to the pandemic. With advances in science, these rates have improved, showing the impact of evidence on practices. As limitations, study sources are cited. It is recommended to carry out new studies. PROSPERO registration CRD42021234486
Fluorescent Markers: Proteins and Nanocrystals
This book chapter will comment on fluorescent reporter proteins and nanocrystalsâ applicability as fluorescent markers. Fluorescent reporter proteins in the Drosophila model system offer a degree of specificity that allows monitoring cellular and biochemical phenomena in vivo, such as autophagy, mitophagy, and changes in the redox state of cells. Titanium dioxide (TiO2) nanocrystals (NCs) have several biological applications and emit in the ultraviolet, with doping of europium ions can be visualized in the red luminescence. Therefore, it is possible to monitor nanocrystals in biological systems using different emission channels. CdSe/CdS magic-sized quantum dots (MSQDs) show high luminescence stability in biological systems and can be bioconjugated with biological molecules. Therefore, this chapter will show exciting results of the group using fluorescent proteins and nanocrystals in biological systems
COVID-19 symptoms at hospital admission vary with age and sex: results from the ISARIC prospective multinational observational study
Background:
The ISARIC prospective multinational observational study is the largest cohort of hospitalized patients with COVID-19. We present relationships of age, sex, and nationality to presenting symptoms.
Methods:
International, prospective observational study of 60â109 hospitalized symptomatic patients with laboratory-confirmed COVID-19 recruited from 43 countries between 30 January and 3 August 2020. Logistic regression was performed to evaluate relationships of age and sex to published COVID-19 case definitions and the most commonly reported symptoms.
Results:
âTypicalâ symptoms of fever (69%), cough (68%) and shortness of breath (66%) were the most commonly reported. 92% of patients experienced at least one of these. Prevalence of typical symptoms was greatest in 30- to 60-year-olds (respectively 80, 79, 69%; at least one 95%). They were reported less frequently in children (â€â18 years: 69, 48, 23; 85%), older adults (â„â70 years: 61, 62, 65; 90%), and women (66, 66, 64; 90%; vs. men 71, 70, 67; 93%, each Pâ<â0.001). The most common atypical presentations under 60 years of age were nausea and vomiting and abdominal pain, and over 60 years was confusion. Regression models showed significant differences in symptoms with sex, age and country.
Interpretation:
This international collaboration has allowed us to report reliable symptom data from the largest cohort of patients admitted to hospital with COVID-19. Adults over 60 and children admitted to hospital with COVID-19 are less likely to present with typical symptoms. Nausea and vomiting are common atypical presentations under 30 years. Confusion is a frequent atypical presentation of COVID-19 in adults over 60 years. Women are less likely to experience typical symptoms than men
Diagnóstico pós-morte de Aelurostrongylus abstrusus e caracterização morfométrica de ovos e mórulas por meio de histologia e impressão de tecido
Os objetivos deste trabalho foram diagnosticar Aelurostrongylus abstrusus por meio das tĂ©cnicas de histologia e impressĂŁo de tecido pulmonar de gato domĂ©stico e realizar a caracterização morfomĂ©trica de ovos e mĂłrulas desse nematĂłdeo. Pela histologia, os ovos (n=100) mediram, em mĂ©dia, 83,4 x 65,5mim, Ăndice morfomĂ©trico (IM) 0,8 e as mĂłrulas (n=100) mediram, em mĂ©dia, 45,4 x 31,8mim, IM 0,7. Pela impressĂŁo de pulmĂŁo, os ovos (n=100) mediram, em mĂ©dia, 105,3 x 93,6mim, IM 0,9, e as mĂłrulas (n=100) mediram, em mĂ©dia, 43,1 x 36,5mim, IM 0,8. A utilização da tĂ©cnica de impressĂŁo de tecido permitiu a observação das mesmas formas parasitĂĄrias visualizadas histologicamente. Os resultados sugerem a viabilidade da utilização dessa tĂ©cnica no diagnĂłstico pĂłs-morte de A. abstrusus