Evaluation of the antioxidant capacity of betalainic fruits and vegetables

The present investigation determined total phenolics, ascorbic acid, betalain contents and the corresponding antioxidant capacities of betalain-bearing fruits and vegetables. In addition to differently coloured Swiss chard petioles (Beta vulgaris L. ssp. cicla [L.] Alef. cv. ‘Bright Lights’) and hypocotyls of white, yellow, and red beetroot varieties (Beta vulgaris L. ssp. vulgaris, cv. ‘Albina Vereduna’, cv. ‘Burpee’s Golden’, and cv. ‘Rote Kugel 2’), juices from cactus pears (Opuntia ficus-indica [L.] Mill. cv. ‘Gialla’ and cv. ‘Rossa’) and pitaya fruits (Hylocereus polyrhizus [Weber] Britton & Rose, H. undatus [Haworth] Britton & Rose, Selenicereus megalanthus [K. Schumann ex Vaupel] Moran) were included in this study. Antioxidant capacities were determined by application of the TEAC and FRAP assays, respectively, resulting in differing rankings of the commodities investigated. In both test systems, highest antioxidant capacity was shown for red beetroot extract while for the remaining samples no straightforward order could be established

Chemical quality parameters and anthocyanin pattern of red-fleshed Weirouge apples

Red-fleshed ‘Weirouge’ apples were investigated with respect to their chemical quality parameters including anthocyanins and colour. Anthocyanin concentrations were considerably higher than previously reported for common red-peeled apples. Due to its high malic acid content, the cultivar ‘Weirouge’ was characterised by a high colour brilliance. Among the anthocyanins, cyanidin-3-maloyl-galactoside and 5-carboxy-pyrano-cyanidin-hexoside not previously found in apples were tentatively identified by HPLC-MS3. Highest anthocyanin and total phenolics contents were found in the peel corresponding with the respective antioxidant capacities as determined using the FRAP and TEAC assays, respectively

Comparison of morphological and chemical fruit traits from different pitaya genotypes (Hylocereus sp.) grown in Costa Rica

Physico-chemical parameters of the fruits of five Hylocereus genotypes grown in their natural habitat in Costa Rica were determined and their morphological traits were assessed. Besides, overall dimensions, shape, weight, flesh fraction, skin thickness, seed content and juice yield were recorded. Chemical analyses comprised pH, total soluble solids, titratable acidity, proline and pectin content, formol number and density of the juices. In addition, the major sugars glucose, fructose and sucrose, as well as organic acids were quantified by HPLC. Moreover, skin and flesh firmness were assessed. Significant differences were observed between genotypes concerning fruit weight and dimensions, skin thickness, proportion of flesh and amount of seeds. Whereas lowest pH values were determined in the juices from ‘San Ignacio’, highest malic acid contents in ‘Rosa’ and maximal total soluble solids were found in juices from ‘Nacional’ fruits. Furthermore, differences between genotypes concerning density, pectin and glucose contents of the juices were noted

Preliminary results of spectral reflectance studies of tycho crater

The preliminary analysis and interpretation of near infrared spectra obtained for both the interior and exterior deposits associated with the Tycho crater is presented. Specific objectives were: (1) to determine the composition and stratigraphy of the highland crust in the Tycho target site; (2) to determine the likely composition of the primary ejecta which may be present in ray deposits; (3) to investigate the nature of spectral units defined in previous studies; (4) to further investigate the nature and origin of both the bright and dark haloes around the rim crest; and (5) to compare the compositions determined for the Tycho units with those of the Aristarchus crater as well as typical highland deposits. The spectra obtained for the interior areas exhibit similar spectral features. These include relatively strong 1 micron absorption bands whose minima are centered between 0.97 and 0.99 microns and shallow to intermediate continuum slopes. The spectra generally exhibit indications of a 1.3 micron feature consistent with the presence of Fe(2+) bearing plagioclase feldspar. The strong 1 micron absorption features indicate a dominant high Ca clinopyroxene component. Results obtained from the ejecta deposits show that the spectrum of the inner, bright halo is almost identical with those obtained for interior units. The spectrum of the dark halo exhibits a wide, relatively shallow absorption feature centered at 1.01 microns, a 1.3 micron absorption, and a steep continuum slope. This spectrum is interpreted as indicating the presence of pyroxene, Fe-bearing feldspar, and a significant component of Fe-bearing impact melt glass. Finally, the spectra of spots inside Tycho show similarity with certain spectra for Aristarchus. However, the suite of spectra obtained for Tycho exhibits a different trend in terms of band center versus width

Crustal heterogeneity of the moon viewed from the Galileo SSI camera: Lunar sample calibrations and compositional implications

Summaries are given of the spectral calibration, compositional parameters, nearside color, and limb and farside color of the Moon. The farside of the Moon, a large area of lunar crust, is dominated by heavily cratered terrain and basin deposits that represent the products of the first half billion years of crustal evolution. Continuing analysis of the returned lunar samples suggest a magma ocean and/or serial magmatism model for evolution of the primordial lunar crust. However, testing either hypothesis requires compositional information about the crustal stratigraphy and lateral heterogeneity. Resolution of this important planetary science issue is dependent on additional data. New Galileo multispectral images indicate previously unknown local and regional compositional diversity of the farside crust. Future analysis will focus on individual features and a more detailed assessment of crustal stratigraphy and heterogeneity

Comparing multifocal pupillographic objective perimetry (mfPOP) and multifocal visual evoked potentials (mfVEP) in retinal diseases

Multifocal pupillographic objective perimetry (mfPOP) shows regions of slight hypersensitivity away from retinal regions damaged by diabetes or age-related macular degeneration (AMD). This study examines if such results also appear in multifocal visual evoked potentials (mfVEPs) recorded on the same day in the same patients. The pupil control system receives input from the extra-striate cortex, so we also examined evidence for such input. We recruited subjects with early type 2 diabetes (T2D) with no retinopathy, and patients with unilateral exudative AMD. Population average responses of the diabetes patients, and the normal fellow eyes of AMD patients, showed multiple regions of significant hypersensitivity (p < 0.05) on both mfPOP and mfVEPs. For mfVEPs the occipital electrodes showed fewer hypersensitive regions than the surrounding electrodes. More advanced AMD showed regions of suppression becoming centrally concentrated in the exudative AMD areas. Thus, mfVEP electrodes biased towards extra-striate cortical responses (surround electrodes) appeared to show similar hypersensitive visual field locations to mfPOP in early stage diabetic and AMD damage. Our findings suggest that hypersensitive regions may be a potential biomarker for future development of AMD or non-proliferative diabetic retinopathy, and may be more informative than visual acuity which remains largely undisturbed during early disease

Rapid Objective Testing of Visual Function Matched to the ETDRS Grid and Its Diagnostic Power in Age-Related Macular Degeneration

Purpose: To study the power of an 80-second multifocal pupillographic objective perimetry (mfPOP) test tailored to the ETDRS grid to diagnose age-related macular degeneration (AMD) by Age-Related Eye Disease Study (AREDS) severity grade. Design: Evaluation of a diagnostic technology. Methods: We compared diagnostic power of acuity, ETDRS grid retinal thickness data, new 80-second M18 mfPOP test, and two wider-field 6-minute mfPOP tests (Macular-P131, Widefield-P129). The M18 stimuli match the size and shape of bifurcated ETDRS grid regions, allowing easy structure–function comparisons. M18, P129, and P131 stimuli test both eyes concurrently. We recruited 34 patients with early-stage AMD with a mean ± standard deviation (SD) age of 72.6 ± 7.06 years. The M18 and P129 plus P131 stimuli had 26 and 51 control participants, respectively with mean ± SD ages of 73.1 ± 8.17 years and 72.1 ± 5.83 years, respectively. Multifocal pupillographic objective perimetry testing used the Food and Drug Administration-cleared Objective FIELD Analyzer (OFA; Konan Medical USA). Main Outcome Measures: Percentage area under the receiver operator characteristic curve (AUC) and Hedge's g effect size. Results: Acuity and OCT ETDRS grid thickness and volume produced reasonable diagnostic power (percentage AUC) for AREDS grade 4 eyes at 83.9 ± 9.98% and 90.2 ± 6.32% (mean ± standard error), respectively, but not for eyes with less severe disease. By contrast, M18 stimuli produced percentage AUCs from 72.8 ± 6.65% (AREDS grade 2) to 92.9 ± 3.93% (AREDS grade 4), and 82.9 ± 3.71% for all eyes. Hedge's g effect sizes ranged from 0.84 to 2.32 (large to huge). Percentage AUC for P131 stimuli performed similarly and for P129 performed somewhat less well. Conclusions: The rapid and objective M18 test provided diagnostic power comparable with that of wider-field 6-minute mfPOP tests. Unlike acuity or OCT ETDRS grid data, OFA tests produced reasonable diagnostic power in AREDS grade 1 to 3 eyes.</p

Stratification of the risk of developing severe or lethal Covid-19 using a new score from a large Italian population: A population-based cohort study

Objectives To develop a population-based risk stratification model (COVID-19 Vulnerability Score) for predicting severe/fatal clinical manifestations of SARS-CoV-2 infection, using the multiple source information provided by the healthcare utilisation databases of the Italian National Health Service. Design Retrospective observational cohort study. Setting Population-based study using the healthcare utilisation database from five Italian regions. Participants Beneficiaries of the National Health Service, aged 18-79 years, who had the residentship in the five participating regions. Residents in a nursing home were not included. The model was built from the 7 655 502 residents of Lombardy region. Main outcome measure The score included gender, age and 29 conditions/diseases selected from a list of 61 conditions which independently predicted the primary outcome, that is, severe (intensive care unit admission) or fatal manifestation of COVID-19 experienced during the first epidemic wave (until June 2020). The score performance was validated by applying the model to several validation sets, that is, Lombardy population (second epidemic wave), and the other four Italian regions (entire 2020) for a total of about 15.4 million individuals and 7031 outcomes. Predictive performance was assessed by discrimination (areas under the receiver operating characteristic curve) and calibration (plot of observed vs predicted outcomes). Results We observed a clear positive trend towards increasing outcome incidence as the score increased. The areas under the receiver operating characteristic curve of the COVID-19 Vulnerability Score ranged from 0.85 to 0.88, which compared favourably with the areas of generic scores such as the Charlson Comorbidity Score (0.60). A remarkable performance of the score on the calibration of observed and predicted outcome probability was also observed. Conclusions A score based on data used for public health management accurately predicted the occurrence of severe/fatal manifestations of COVID-19. Use of this score may help health decision-makers to more accurately identify high-risk citizens who need early preventive or treatment interventions