Use of Phytotherapics, Low Power Laser and Ozone for Biting Wound in Dog

Background: Skin wounds are among the most common cases in small animals, highlighting the importance of alternative therapies as an aid to the healing process. These include ozone, which has a bactericidal and immunomodulatory action; the low power laser that determines photodynamic changes triggering metabolism elevation; and the use of medicinal plants, in which several herbal medicines has been mentioned in research relating their benefits to wound healing in humans and animals. Thus, it aims to report the case of a dog submitted to these treatments due to chronic wound due to bite.Case: A 6-year-old canine, female, mixed-breed, 8 kg, with a perforating wound in the left anterior limb in the scapular and humeral region, caused by trauma from the bite of another dog, previously treated, without clinical improvement, has been attended presenting apathy, increased body temperature (40.1°C), difficulty in walking and inability to support the injured limb, which was swollen, hyperemic, with elevated temperature, absence of hair, elevated pain sensitivity and presence of 2 circular lesions, approximately 2 cm in diameter, caused by trauma, and others of size between 0.5 and 1 cm in diameter, which appeared during the course of the disease, characterized as fistulas, through which there was drainage of characteristic fluid exudative and purulent discharge. It was collected blood sample for blood count and purulent material through sterile swab for culture and antibiogram. Systemic treatment with ceftiofur, dipyrone, meloxican and topical cleaning of the wounds with hydrogen peroxide and physiological solution; dimethyl sulfoxide ointment, and hot compresses was chosen. After seven days, the animal showed significant general clinical improvement and in the limb, it was observed: decreased edema, hyperemia, hyperthermia and secretions, with no pus. Only local treatment was recommended, with cleansing of the skin lesions, with physiological solution, chlorhexidine and healing gel made of herbal medicine: chitosan, aloe vera, marigold, sunflower oil and barbatimao. After 21 days of treatment, the injured limb showed consecutive clinical improvement, with epithelialization of the smaller diameter lesions. Low-level laser and ozone therapy was instituted, totaling three initial sessions every five days and one last session 14 days after the third. After the four sessions the animal was discharged, presenting absence of the previously reported alterations, besides the total re-epithelialization of the lesions and hair growth. Discussion: The contamination arising from bite can interfere with healing time due to the action of bacterial toxins and inflammatory infiltrates that cause vascular and cellular damage. According to other reports the use of ozone therapy, local and systemic, promoted rapid healing, related to effective decontamination, in a short period of time. Considering the response and quality of tissue repair, due to wound recovery, verifying therapeutic effects of anti-inflammatory action, effective decontamination, stimulation of granulation tissue, epithelialization and repilation of the cutaneous region, it is concluded that topical and systemic ozone therapy, associated with the effect of tissue regeneration by edge revival in chronic lesions, of low power laser and herbal medicine gel, under the conditions of this case, represent satisfactory therapy for the treatment of bite wounds in dogs

Produção de sementes de capim‑mombaça submetido a diferentes datas de rebaixamento e doses de nitrogênio

The objective of this work was to evaluate the effects of closing cut dates (CCD) and nitrogen (N) rates on the components of pure seed yield (PSY) and seed quality in 'Mombasa' grass (Megathyrsus maximus 'Mombaça'), besides determining the parameters of apparent N efficiency (ANE) and agronomic N efficiency (AgNE). The field experiments were carried out over two growing seasons, 2010/2011 and 2011/2012, in Umuarama, PR, Brazil. The folllowing treatments were evaluated: CCD on October 10, January 31, February 15, and March 1; and N doses at 0, 75, 150, and 225 kg ha-1. Significant interactions between the CCD and N doses were observed for the panicle tiller number (PTN), pure seeds per panicle, PSY, ANE and AgNE. Values of ANE and AgNE were better for 150 kg N ha-1. There were no effects of CCD and N doses on seed quality. PSY was closely correlated with PTN, pure seeds per panicle, and pure seed number.The CCD treatments of longer growth duration and higher N doses showed the higher PSY. Final CCD delaying until February and N availability reduced yield. Closing cut date and N fertilization affect pure seed yield and yield component responses of 'Mombasa' grass.O objetivo deste trabalho foi avaliar o efeito de datas de rebaixamento (DR) e doses de nitrogênio (N) sobre os componente de rendimento de sementes puras (RSP) e de qualidade de sementes em capim‑mombaça (Megathyrsus maximus 'Mombaça'), além de determinar os parâmetros de eficiência aparente do nitrogênio (EAN) e eficiência agronômica de nitrogênio (EAgN). Os experimentos foram realizados nas estações de crescimento em 2010/2011 e 2011/2012, em Umuarama, PR, Brasil. Os seguintes tratamentos foram avaliados: DR em 10 de outubro, 31 de janeiro, 15 de fevereiro e 1 de março; e as doses 0, 75, 150 e 225 kg ha-1 de N. Verificou-se interação significativa entre DR e doses de N quanto ao número de perfilhos com panículas (NPP), sementes puras por panícula, RSP, EAN e EAgN. Os valores de EAN e EAgN foram melhores com 150 kg ha-1 de N. A DR e o N não tiveram efeitos sobre a qualidade das sementes. O RSP foi estreitamente correlacionado ao NPP, às sementes puras por panícula e ao número de sementes puras. As DRs com maiores durações de crescimento e doses de N tiveram maiores RSP. O atraso da DR final até fevereiro e a disponibilidade de N reduziram os rendimentos. A data de rebaixamento e a adubação com N afetam as respostas do rendimento de sementes puras e as componentes do rendimento do capim‑mombaça

UTILIZAÇÃO DE SULFATO DE COBRE PARA DEBRIDAMENTO DO TECIDO DE GRANULAÇÃO EXUBERANTE EM FERIDA TRAUMÁTICA DE EQUINO

Due to the facilities in which horses are reared and their nature, they usually are involved in accidents that most of the times result in limb injuries of varying degrees of severity. Wounds located at the distal extremities are generally complicated by lack of lining tissue, poor circulation, joint movement, increased predisposition to contamination and consequent infection. Furthermore, skin healing in horses have particular characteristics, with exuberant granulation tissue formation being common in this species, leading to a longer time for clinical recovery and wound resolution. Several treatments have been described in the literature to prevent or reduce exuberant granulation in horses. However, no protocol has been described for the treatment of chronic wounds in this species. This paper aims at reporting two cases of horses with lacerating wounds in the hind limbs, with exuberant granulation growth, topically treated with 20% copper sulfate. This method of treatment has been shown to be effective, inexpensive, and easy to perform, being a possible therapy for chronic wounds with hypergranulation tissue in horses, especially in cases where surgical resection of the tissue is not possible.Devido às instalações em que são criados e à sua natureza, os equinos comumente envolvem-se em acidentes que resultam, quase que em sua totalidade, em lesões nos membros em variados níveis de gravidade. Feridas localizadas nas extremidades distais são, em geral, complicadas pela falta de tecido de revestimento, má circulação, movimento articular, maior predisposição para contaminação e consequente infecção. Além disso os equinos apresentam particularidades relacionadas ao processo de cicatrização cutânea, sendo comum nessa espécie a formação de tecido de granulação exuberante, acarretando um tempo maior para a recuperação clínica e resolução da ferida. Diversos tratamentos têm sido descritos na literatura com o objetivo de prevenir ou reduzir a granulação exuberante em equinos, porém, ainda não há descrição de um protocolo para o tratamento de feridas crônicas nessa espécie. O objetivo deste trabalho é relatar dois casos de equinos com feridas lacerantes em membros pélvicos, com o crescimento de tecido de granulação exuberante, tratado topicamente com sulfato de cobre a 20%. Esse método de tratamento mostrou-se eficaz, de baixo custo e fácil realização, constituindo uma possível terapia para feridas crônicas com hipergranulação em cavalos, principalmente nos casos em que a ressecção cirúrgica do tecido não for possível de ser realizada

Use of Calender Extract Cream in Equine Lacerate Wound

Background: Wounds and lacerations are constant in the clinical routine of horses. The treatment of wounds by the second intention is the most common in the equine clinic; it consists in addition to surgical debridement, the use of allopathic or phytotherapeutic healing. In this context, several phytotherapics used in folk medicine have been used with promising results in the equine species, especially marigold (Calendula officinalis L.), recognized by the German scientific committee as antiseptic and healing. The objective of this study was to evaluate the efficacy of the nonionic cream of Calendula officinalis at 2% in an extensive lacerating wound in an equine.Case: A 6-month-old female Quarter Horse, weighing 160 kg, a lacerating wound in the pectoral region of unknown traumatic origin, was treated in the large animal sector of the Veterinary Hospital of Universidade Paranaense (UNIPAR). Clinical examination revealed an exudative wound in the pectoral region, complicated by the presence of environmental organic matter and myiasis, involving transverse pectoral and descending pectoral muscles, with a large tissue separation and exposure of part of the first sternum. After an initial evaluation, a trichotomy was performed around the wound, removal of myiasis and sanitization of the wound with water and PVP-I, which was filled with sterile dressings and covered with a dressing using a tubular mesh. Antibiotic therapy was instituted, along with anti-inflammatory medication and antitetanic serum (5,000 IU). Due to the need for surgical debridement, after previous fasting and general anesthesia followed by site antisepsis, surgical debridement was performed, followed by reduction of dead space with application of standard "Wolf" sutures, for re-approximation of the wound edges was applied suture in Wolf pattern captonated with segments of equipment. In the post-surgical period, topical treatment with nitrofurazone ointment and crystal sugar was instituted. However, after five days of the surgical procedure, there was dehiscence of the wound, at which time another intervention was chosen. After sedation remaining in standard anatomical position, sutures of the same pattern and wire were applied, but captonated with polyester buds and wound dressing was instituted, followed by the application of nonionic cream of marigold (C. officinalis) extract, in the concentration of 2%, twice a day until complete healing of the wound.Discussion: The use of nonionic marigold cream (C. officinalis) 2% showed a significant efficacy, in the case reported, corroborating with literature data that marigold presented an advantage in the inflammatory phase of the healing process, when compared to the other two substances, favored epithelization and demonstrated bactericidal action. This activity is reported to the substances present in C. officinalis, especially the flavonoids, which are phenolic compounds derived from the secondary metabolism of plants. In addition to phenolic compounds such as triterpenes, faradiol esters and taraxasterol are considered responsible for antioxidant, anti-inflammatory, antimicrobial and angiogenic activities, actions that contribute directly or indirectly to the healing process. The present report contributes positively to the use of alternative treatments in wounds as the phytotherapeutic in question, since it proved feasible, besides avoiding the use of allopathic ones with higher associated costs and environmental impact in its production and use, as well as the emergence of bacterial resistance

Uso de retalho de padrão subdérmico de rotação após exérese de neoplasia craniana, associada à ablação do conduto auditivo em cão – Relato de caso/ Use of a subdermal pattern flap of rotation after cranial neoplasia excision, associated with ablation of the auditory canal in dog – Case report

As neoplasias são as principais causas de morte nos cães e gatos; cerca de 50% dos cães e 35% dos gatos são acometidos por algum processo neoplásico durante a vida. A intervenção cirúrgica constitui-se em uma das principais modalidades de tratamento para os tumores passíveis de remoção completa, sendo um dos procedimentos mais eficazes quando se tratam de tumores grandes e localizados. A aplicabilidade da cirurgia reconstrutiva como correção cirúrgica é uma boa opção. As técnicas de correções realizadas em cirurgias reconstrutivas são retalhos e enxertos. A grande vantagem de empregar retalhos cutâneos é o fato de eles permitirem a cobertura imediata da região afetada e reduzirem o tempo de cicatrização do tecido, proporcionando ao paciente melhores resultados estéticos e funcionais. Por meio de tomografia computadorizada constatou-se a presença de massa em região temporal e cervical superficial lateral esquerda. Portanto, o presente trabalho teve como objetivo relatar o emprego da cirurgia reconstrutiva, após a excisão de um tumor na cabeça aderido ao crânio e orelha média e externa, associado a ablação do conduto auditivo, utilizando um retalho de padrão subdérmico de rotação em cão

Utilização de creme de papaína e extrato liofilizado das raízes de salsa (Petroselinum crispum) contra Escherichia coli multirresistente em abscesso em equino

Background: Due to the bacterial resistance to conventional antibiotics, studies on natural products with antibacterial or bactericidal activity are becoming more and more frequent. Among multi-resistant bacteria, Escherichia coli is a producer of extended-spectrum beta-lactamase (ESBL). Papain, coming from papaya latex (Carica papaya), stands out for its capacity to degrade the devitalized tissue that delays the healing process. Parsley (Petroselinum crispum) has been studied, mainly for its diuretic, antioxidant and antimicrobial properties. The aim of this work was to report the use of these two phytotherapic agents in an equine presenting abscess infected by multi-resistant ESBL producing E. coli.Case: A four and a half year old male neutered equine weighing 400 kg of undefined race (SRD) was admitted to the Veterinary Hospital of the Paranaense University (UNIPAR), presenting an increased volume on the left side of the middle third of the neck, one year ago, probably due to an intramuscular injection in the splenius muscle performed by the animal’s owner, who did not remember which drug had been applied. Physical examination revealed a characteristic abscess lesion that involved the subcutaneous and muscular tissue of approximately 10 cm in diameter, and presented a fistulous trajectory with purulent content drainage and pain upon palpation. The animal was initially submitted to surgical drainage of the abscess and to systemic treatment with ceftiofur. In view of the failure of the therapy proposed, the lesion was treated with 2% papain cream and, subsequently, in association with lyophilized parsley extract (Petroselinum crispum) after the identification of the presence of multi-resistant ESBL producing E. coli, isolated from the lesion and identified through standard laboratory tests. The use of 2% papain cream reduced the inflammatory process and fibrous tissue. Lyophilized parsley root extract was effective against infection by multi-resistant bacteria.Discussion: The reduction of purulent secretion and the size of the inflammatory process are due to the proteolytic activity of papain, which decreases bacterial adhesion, and the degree of wound contamination, promoting healing. Several studies have indicated the bacteriostatic and bactericidal activity of papain, but in some trials, this effect was only found in a concentration of 10% for Pseudomonas aeruginosa and Staphylococcus aureus, and no effect for E. coli bacteria. In vitro studies have shown that the essential oil of Petroselinum crispum leaves and stems is more effective than the essential oil of seeds against this bacterium. Reports of isolation and bacterial identification of wounds and abscesses of horses present a large amount of microorganisms, but the presence of multi-resistant E. coli is uncommon. In other countries, the presence of this bacterium in wounds and other regions has been described in this species. In the Netherlands and Germany, the presence of ESBL producing E. coli in horses was detected in clinical samples of pus, stomach, uterus, feces and synovial fluid, presenting a real risk of human contamination by these resistant bacteria. In this sense, the study of antimicrobial agents has great scope, being a crucial point in several sectors of the pharmaceutical field. The phytotherapeutic association, used in this case, represents a possible promising, low-cost alternative for the treatment of contaminated wounds, emphasizing the importance of studies involving alternative treatments for bacterial resistance

Seed production of 'Mombasa' grass subjected to different closing cut dates and nitrogen rates

Abstract -The objective of this work was to evaluate the effects of closing cut dates (CCD) and nitrogen (N) rates on the components of pure seed yield (PSY) and seed quality in 'Mombasa' grass (Megathyrsus maximus 'Mombaça'), besides determining the parameters of apparent N efficiency (ANE) and agronomic N efficiency (AgNE). The field experiments were carried out over two growing seasons, 2010/2011 and 2011/2012, in Umuarama, PR, Brazil. The folllowing treatments were evaluated: CCD on October 10, January 31, February 15, and March 1; and N doses at 0, 75, 150, and 225 kg ha -1 . Significant interactions between the CCD and N doses were observed for the panicle tiller number (PTN), pure seeds per panicle, PSY, ANE and AgNE. Values of ANE and AgNE were better for 150 kg N ha -1 . There were no effects of CCD and N doses on seed quality. PSY was closely correlated with PTN, pure seeds per panicle, and pure seed number.The CCD treatments of longer growth duration and higher N doses showed the higher PSY. Final CCD delaying until February and N availability reduced yield. Closing cut date and N fertilization affect pure seed yield and yield component responses of 'Mombasa' grass. Index terms: agronomic nitrogen efficiency, apparent nitrogen efficiency, seed quality, seed yield. de N. A DR e o N não tiveram efeitos sobre a qualidade das sementes. O RSP foi estreitamente correlacionado ao NPP, às sementes puras por panícula e ao número de sementes puras. As DRs com maiores durações de crescimento e doses de N tiveram maiores RSP. O atraso da DR final até fevereiro e a disponibilidade de N reduziram os rendimentos. A data de rebaixamento e a adubação com N afetam as respostas do rendimento de sementes puras e as componentes do rendimento do capim-mombaça. Produção de sementes de capim-mombaça submetido a diferentes datas de rebaixamento e doses de nitrogênio Termos para indexação: eficiência agronômica de nitrogênio, eficiência aparente de nitrogênio, qualidade de sementes, rendimento de sementes