274 research outputs found
Quorum sensing influences growth and photosynthetic membrane production in high-cell-density cultivations of Rhodospirillum rubrum
BACKGROUND: The facultative anoxygenic photosynthetic bacterium Rhodospirillum rubrum exhibits versatile metabolic activity allowing the adaptation to rapidly changing growth conditions in its natural habitat, the microaerobic and anoxic zones of stagnant waters. The microaerobic growth mode is of special interest as it allows the high-level expression of photosynthetic membranes when grown on succinate and fructose in the dark, which could significantly simplify the industrial production of compounds associated with PM formation. However, recently we showed that PM synthesis is no longer inducible when R. rubrum cultures are grown to high cell densities under aerobic conditions. In addition a reduction of the growth rate and the continued accumulation of precursor molecules for bacteriochlorophyll synthesis were observed under high cell densities conditions. RESULTS: In the present work, we demonstrate that the cell density-dependent effects are reversible if the culture supernatant is replaced by fresh medium. We identified six N-acylhomoserine lactones and show that four of them are produced in varying amounts according to the growth phase and the applied growth conditions. Further, we demonstrate that N-acylhomoserine lactones and tetrapyrrole compounds released into the growth medium affect the growth rate and PM expression in high cell density cultures. CONCLUSIONS: In summary, we provide evidence that R. rubrum possesses a Lux-type quorum sensing system which influences the biosynthesis of PM and the growth rate and is thus likely to be involved in the phenotypes of high cell density cultures and the rapid adaptation to changing environmental conditions
Optical Properties of Deep Ice at the South Pole - Absorption
We discuss recent measurements of the wavelength-dependent absorption
coefficients in deep South Pole ice. The method uses transit time distributions
of pulses from a variable-frequency laser sent between emitters and receivers
embedded in the ice. At depths of 800 to 1000 m scattering is dominated by
residual air bubbles, whereas absorption occurs both in ice itself and in
insoluble impurities. The absorption coefficient increases approximately
exponentially with wavelength in the measured interval 410 to 610 nm. At the
shortest wavelength our value is about a factor 20 below previous values
obtained for laboratory ice and lake ice; with increasing wavelength the
discrepancy with previous measurements decreases. At around 415 to 500 nm the
experimental uncertainties are small enough for us to resolve an extrinsic
contribution to absorption in ice: submicron dust particles contribute by an
amount that increases with depth and corresponds well with the expected
increase seen near the Last Glacial Maximum in Vostok and Dome C ice cores. The
laser pulse method allows remote mapping of gross structure in dust
concentration as a function of depth in glacial ice.Comment: 26 pages, LaTex, Accepted for publication in Applied Optics. 9
figures, not included, available on request from [email protected]
Повышение эффективности коррозионной защиты магистральных трубопроводов в грунтах с высокой коррозионной активностью
В работе изложен анализ существующих методов борьбы с коррозионными разрушениями трубопроводов в грунтах с высокой коррозионной активностью. В процессе исследования проводились: изучение теоретических основ процессов коррозионных разрушений; выявление факторов, оказывающих наибольшее влияние на возникновение коррозионных процессов; рассмотрение методов пассивной и активной защиты трубопроводов от коррозии.
В результате исследования: произведен расчет электрохимической защиты.
Область применения: линейная часть магистрального трубопровода.The article presents an analysis of existing methods for combating corrosion damage to pipelines in soils with increased corrosive activity. In the course of the research, the following were carried out: study of the theoretical foundations of corrosion destruction processes; identification of factors that have the greatest impact on the occurrence of corrosion processes; consideration of methods of passive and active protection of pipelines from corrosion. As a result of the research: the calculation of the electrochemical protection was made. Scope: linear part of the main pipeline
The AMANDA Neutrino Telescope and the Indirect Search for Dark Matter
With an effective telescope area of order 10^4 m^2, a threshold of ~50 GeV
and a pointing accuracy of 2.5 degrees, the AMANDA detector represents the
first of a new generation of high energy neutrino telescopes, reaching a scale
envisaged over 25 years ago. We describe its performance, focussing on the
capability to detect halo dark matter particles via their annihilation into
neutrinos.Comment: Latex2.09, 16 pages, uses epsf.sty to place 15 postscript figures.
Talk presented at the 3rd International Symposium on Sources and Detection of
Dark Matter in the Universe (DM98), Santa Monica, California, Feb. 199
The AMANDA Neutrino Telescope: Principle of Operation and First Results
AMANDA is a high-energy neutrino telescope presently under construction at
the geographical South Pole. In the Antarctic summer 1995/96, an array of 80
optical modules (OMs) arranged on 4 strings (AMANDA-B4) was deployed at depths
between 1.5 and 2 km. In this paper we describe the design and performance of
the AMANDA-B4 prototype, based on data collected between February and November
1996. Monte Carlo simulations of the detector response to down-going
atmospheric muon tracks show that the global behavior of the detector is
understood. We describe the data analysis method and present first results on
atmospheric muon reconstruction and separation of neutrino candidates. The
AMANDA array was upgraded with 216 OMs on 6 new strings in 1996/97
(AMANDA-B10), and 122 additional OMs on 3 strings in 1997/98.Comment: 36 pages, 23 figures, submitted to Astroparticle Physic
Limits on diffuse fluxes of high energy extraterrestrial neutrinos with the AMANDA-B10 detector
Data from the AMANDA-B10 detector taken during the austral winter of 1997
have been searched for a diffuse flux of high energy extraterrestrial
muon-neutrinos, as predicted from, e.g., the sum of all active galaxies in the
universe. This search yielded no excess events above those expected from the
background atmospheric neutrinos, leading to upper limits on the
extraterrestrial neutrino flux. For an assumed E^-2 spectrum, a 90% classical
confidence level upper limit has been placed at a level E^2 Phi(E) = 8.4 x
10^-7 GeV cm^-2 s^-1 sr^-1 (for a predominant neutrino energy range 6-1000 TeV)
which is the most restrictive bound placed by any neutrino detector. When
specific predicted spectral forms are considered, it is found that some are
excluded.Comment: Submitted to Physical Review Letter
Muon Track Reconstruction and Data Selection Techniques in AMANDA
The Antarctic Muon And Neutrino Detector Array (AMANDA) is a high-energy
neutrino telescope operating at the geographic South Pole. It is a lattice of
photo-multiplier tubes buried deep in the polar ice between 1500m and 2000m.
The primary goal of this detector is to discover astrophysical sources of high
energy neutrinos. A high-energy muon neutrino coming through the earth from the
Northern Hemisphere can be identified by the secondary muon moving upward
through the detector. The muon tracks are reconstructed with a maximum
likelihood method. It models the arrival times and amplitudes of Cherenkov
photons registered by the photo-multipliers. This paper describes the different
methods of reconstruction, which have been successfully implemented within
AMANDA. Strategies for optimizing the reconstruction performance and rejecting
background are presented. For a typical analysis procedure the direction of
tracks are reconstructed with about 2 degree accuracy.Comment: 40 pages, 16 Postscript figures, uses elsart.st
Search for Point Sources of High Energy Neutrinos with AMANDA
This paper describes the search for astronomical sources of high-energy
neutrinos using the AMANDA-B10 detector, an array of 302 photomultiplier tubes,
used for the detection of Cherenkov light from upward traveling
neutrino-induced muons, buried deep in ice at the South Pole. The absolute
pointing accuracy and angular resolution were studied by using coincident
events between the AMANDA detector and two independent telescopes on the
surface, the GASP air Cherenkov telescope and the SPASE extensive air shower
array. Using data collected from April to October of 1997 (130.1 days of
livetime), a general survey of the northern hemisphere revealed no
statistically significant excess of events from any direction. The sensitivity
for a flux of muon neutrinos is based on the effective detection area for
through-going muons. Averaged over the Northern sky, the effective detection
area exceeds 10,000 m^2 for E_{mu} ~ 10 TeV. Neutrinos generated in the
atmosphere by cosmic ray interactions were used to verify the predicted
performance of the detector. For a source with a differential energy spectrum
proportional to E_{nu}^{-2} and declination larger than +40 degrees, we obtain
E^2(dN_{nu}/dE) <= 10^{-6}GeVcm^{-2}s^{-1} for an energy threshold of 10 GeV.Comment: 46 pages, 22 figures, 4 tables, submitted to Ap.
Results from the Antarctic Muon and Neutrino Detector Array (AMANDA)
We show new results from both the older and newer incarnations of AMANDA
(AMANDA-B10 and AMANDA-II, respectively). These results demonstrate that AMANDA
is a functioning, multipurpose detector with significant physics and
astrophysics reach. They include a new higher-statistics measurement of the
atmospheric muon neutrino flux and preliminary results from searches for a
variety of sources of ultrahigh energy neutrinos: generic point sources,
gamma-ray bursters and diffuse sources producing muons in the detector, and
diffuse sources producing electromagnetic or hadronic showers in or near the
detector.Comment: Invited talk at the XXth International Conference on Neutrino Physics
and Astrophysics (Neutrino 2002), Munich, Germany, May 25-30, 200
Limits to the muon flux from WIMP annihilation in the center of the Earth with the AMANDA detector
A search for nearly vertical up-going muon-neutrinos from neutralino
annihilations in the center of the Earth has been performed with the AMANDA-B10
neutrino detector. The data sample collected in 130.1 days of live-time in
1997, ~10^9 events, has been analyzed for this search. No excess over the
expected atmospheric neutrino background is oberved. An upper limit at 90%
confidence level on the annihilation rate of neutralinos in the center of the
Earth is obtained as a function of the neutralino mass in the range 100
GeV-5000 GeV, as well as the corresponding muon flux limit.Comment: 14 pages, 11 figures. Version accepted for publication in Physical
Review
- …