The caloron correspondence and higher string classes for loop groups

We review the caloron correspondence between $G$-bundles on $M \times S^1$ and $\Omega G$-bundles on $M$, where $\Omega G$ is the space of smooth loops in the compact Lie group $G$. We use the caloron correspondence to define characteristic classes for $\Omega G$-bundles, called string classes, by transgression of characteristic classes of $G$-bundles. These generalise the string class of Killingback to higher dimensional cohomology.Comment: 21 pages. Author addresses adde

Case of severe hypertension and nephrotic range proteinuria

No abstract available

A Distribution of Large Particles in the Coma of Comet 103P/Hartley 2

The coma of comet 103P/Hartley 2 has a significant population of large particles observed as point sources in images taken by the Deep Impact spacecraft. We measure their spatial and flux distributions, and attempt to constrain their composition. The flux distribution of these particles implies a very steep size distribution with power-law slopes ranging from -6.6 to -4.7. The radii of the particles extend up to 20 cm, and perhaps up to 2 m, but their exact sizes depend on their unknown light scattering properties. We consider two cases: bright icy material, and dark dusty material. The icy case better describes the particles if water sublimation from the particles causes a significant rocket force, which we propose as the best method to account for the observed spatial distribution. Solar radiation is a plausible alternative, but only if the particles are very low density aggregates. If we treat the particles as mini-nuclei, we estimate they account for <16-80% of the comet's total water production rate (within 20.6 km). Dark dusty particles, however, are not favored based on mass arguments. The water production rate from bright icy particles is constrained with an upper limit of 0.1 to 0.5% of the total water production rate of the comet. If indeed icy with a high albedo, these particles do not appear to account for the comet's large water production rate. production rate. Erratum: We have corrected the radii and masses of the large particles of comet 103P/Hartley 2 and present revised conclusions in the attached erratum.Comment: Original article: 46 pages, 17 figures, 5 tables, published in Icarus. Erratum: 5 pages, 1 table, accepted for publication in Icaru

Automethylation of G9a and its implication in wider substrate specificity and HP1 binding.

Methylation of lysine residues on histones participates in transcriptional gene regulation. Lysine 9 methylation of histone H3 is a transcriptional repression signal, mediated by a family of SET domain containing AdoMet-dependent enzymes. G9a methyltransferase is a euchromatic histone H3 lysine 9 methyltransferase. Here, G9a is shown to methylate other cellular proteins, apart from histone H3, including automethylation of K239 residue. Automethylation of G9a did not impair or activate the enzymatic activity in vitro. The automethylation motif of G9a flanking target K239 (ARKT) has similarity with histone H3 lysine 9 regions (ARKS), and is identical to amino acids residues in EuHMT (ARKT) and mAM (ARKT). Under steady-state kinetic assay conditions, full-length G9a methylates peptides representing ARKS/T motif of H3, G9a, mAM and EuHMT efficiently. Automethylation of G9a at ARKT motif creates a binding site for HP1 class of protein and mutation of lysine in the motif impairs this binding. In COS-7 cells GFP fusion of the wild-type G9a co-localized with HP1alpha and HP1gamma isoforms whereas the G9a mutant with K239A displayed poor co-localization. Thus, apart from transcriptional repression and regulatory roles of lysine methylation, the non-histone protein methylation may create binding sites for cellular protein-protein interactions

GALEX Observations of CS and OH Emission in Comet 9P/Tempel 1 During Deep Impact

GALEX observations of comet 9P/Tempel 1 using the near ultraviolet (NUV) objective grism were made before, during and after the Deep Impact event that occurred on 2005 July 4 at 05:52:03 UT when a 370 kg NASA spacecraft was maneuvered into the path of the comet. The NUV channel provides usable spectral information in a bandpass covering 2000 - 3400 A with a point source spectral resolving power of approximately 100. The primary spectral features in this range include solar continuum scattered from cometary dust and emissions from OH and CS molecular bands centered near 3085 and 2575 A, respectively. In particular, we report the only cometary CS emission detected during this event. The observations allow the evolution of these spectral features to be tracked over the period of the encounter. In general, the NUV emissions observed from Tempel 1 are much fainter than those that have been observed by GALEX from other comets. However, it is possible to derive production rates for the parent molecules of the species detected by GALEX in Tempel 1 and to determine the number of these molecules liberated by the impact. The derived quiescent production rates are Q(H2O) = 6.4e27 molecules/s and Q(CS2) = 6.7e24 molecules/s, while the impact produced an additional 1.6e32 H2O molecules and 1.3e29 CS2 molecules, a similar ratio as in quiescent outgassing.Comment: 15 pages, 4 figures, accepted for publication in the Astrophysical Journa

Evolution of histone 2A for chromatin compaction in eukaryotes.

During eukaryotic evolution, genome size has increased disproportionately to nuclear volume, necessitating greater degrees of chromatin compaction in higher eukaryotes, which have evolved several mechanisms for genome compaction. However, it is unknown whether histones themselves have evolved to regulate chromatin compaction. Analysis of histone sequences from 160 eukaryotes revealed that the H2A N-terminus has systematically acquired arginines as genomes expanded. Insertion of arginines into their evolutionarily conserved position in H2A of a small-genome organism increased linear compaction by as much as 40%, while their absence markedly diminished compaction in cells with large genomes. This effect was recapitulated in vitro with nucleosomal arrays using unmodified histones, indicating that the H2A N-terminus directly modulates the chromatin fiber likely through intra- and inter-nucleosomal arginine-DNA contacts to enable tighter nucleosomal packing. Our findings reveal a novel evolutionary mechanism for regulation of chromatin compaction and may explain the frequent mutations of the H2A N-terminus in cancer

The general caloron correspondence

We outline in detail the general caloron correspondence for the group of automorphisms of an arbitrary principal $G$-bundle $Q$ over a manifold $X$, including the case of the gauge group of $Q$. These results are used to define characteristic classes of gauge group bundles. Explicit but complicated differential form representatives are computed in terms of a connection and Higgs field.Comment: 25 pages. New section added containing example

INO80C Remodeler Maintains Genomic Stability by Preventing Promiscuous Transcription at Replication Origins

The proper coordination of transcription with DNA replication and repair is central for genomic stability. We investigate how the INO80C chromatin remodeling enzyme might coordinate these genomic processes. We find that INO80C co-localizes with the origin recognition complex (ORC) at yeast replication origins and is bound to replication initiation sites in mouse embryonic stem cells (mESCs). In yeast, INO80C recruitment requires origin sequences but does not require ORC, suggesting that recruitment is independent of pre-replication complex assembly. In both yeast and ESCs, INO80C co-localizes at origins with Mot1 and NC2 transcription factors, and genetic studies suggest that they function together to promote genome stability. Interestingly, nascent transcript sequencing demonstrates that INO80C and Mot1 prevent pervasive transcription through origin sequences, and absence of these factors leads to formation of new DNA double-strand breaks. We propose that INO80C and Mot1/NC2 function through distinct pathways to limit origin transcription, maintaining genomic stability

Comparison of Structure and Properties of Femtosecond and Nanosecond Laser-Structured Silicon

We compare the optical properties, chemical composition, and crystallinity of silicon microstructures formed in the presence of SF6 by femtosecond laser irradiation and by nanosecond laser irradiation. In spite of very different morphology and crystallinity, the optical properties and chemical composition of the two types of microstructures are very similar. The structures formed with femtosecond (fs) pulses are covered with a disordered nanocrystalline surface layer less than 1 um thick, while those formed with nanosecond (ns) pulses have very little disorder. Both ns-laser-formed and fs-laser-formed structures absorb near-infrared (1.1 – 2.5 um) radiation strongly and have roughly 0.5% sulfur impurities.Engineering and Applied Science