A dual paper-based nucleic acid extraction method from blood in under ten minutes for point-of-care diagnostics.

Nucleic acid extraction (NAE) plays a crucial role for diagnostic testing procedures. For decades, dried blood spots (DBS) have been used for serology, drug monitoring, and molecular studies. However, extracting nucleic acids from DBS remains a significant challenge, especially when attempting to implement these applications to the point-of-care (POC). To address this issue, we have developed a paper-based NAE method using cellulose filter papers (DBSFP) that operates without the need for electricity (at room temperature). Our method allows for NAE in less than 7 min, and it involves grade 3 filter paper pre-treated with 8% (v/v) igepal surfactant, 1 min washing step with 1× PBS, and 5 min incubation at room temperature in 1× TE buffer. The performance of the methodology was assessed with loop-mediated isothermal amplification (LAMP), targeting the human reference gene beta-actin and the kelch 13 gene from P. falciparum. The developed method was evaluated against FTA cards and magnetic bead-based purification, using time-to-positive (min) for comparative analysis. Furthermore, we optimised our approach to take advantage of the dual functionality of the paper-based extraction, allowing for elution (eluted disk) as well as direct placement of the disk in the LAMP reaction (in situ disk). This flexibility extends to eukaryotic cells, bacterial cells, and viral particles. We successfully validated the method for RNA/DNA detection and demonstrated its compatibility with whole blood stored in anticoagulants. Additionally, we studied the compatibility of DBSFP with colorimetric and lateral flow detection, showcasing its potential for POC applications. Across various tested matrices, targets, and experimental conditions, our results were comparable to those obtained using gold standard methods, highlighting the versatility of our methodology. In summary, this manuscript presents a cost-effective solution for NAE from DBS, enabling molecular testing in virtually any POC setting. When combined with LAMP, our approach provides sample-to-result detection in under 35 minutes

High-dimensional decoy-state quantum key distribution over 0.3 km of multicore telecommunication optical fibers

Multiplexing is a strategy to augment the transmission capacity of a communication system. It consists of combining multiple signals over the same data channel and it has been very successful in classical communications. However, the use of enhanced channels has only reached limited practicality in quantum communications (QC) as it requires the complex manipulation of quantum systems of higher dimensions. Considerable effort is being made towards QC using high-dimensional quantum systems encoded into the transverse momentum of single photons but, so far, no approach has been proven to be fully compatible with the existing telecommunication infrastructure. Here, we overcome such a technological challenge and demonstrate a stable and secure high-dimensional decoy-state quantum key distribution session over a 0.3 km long multicore optical fiber. The high-dimensional quantum states are defined in terms of the multiple core modes available for the photon transmission over the fiber, and the decoy-state analysis demonstrates that our technique enables a positive secret key generation rate up to 25 km of fiber propagation. Finally, we show how our results build up towards a high-dimensional quantum network composed of free-space and fiber based linksComment: Please see the complementary work arXiv:1610.01812 (2016

The challenge of modelling nitrogen management at the field scale : simulation and sensitivity analysis of N2O fluxes across nine experimental sites using DailyDayCent

Peer reviewedPublisher PD

Glass transition and effective potential in the hypernetted chain approximation

We study the glassy transition for simple liquids in the hypernetted chain (HNC) approximation by means of an effective potential recently introduced. Integrating the HNC equations for hard spheres, we find a transition scenario analogous to that of the long range disordered systems with ``one step replica symmetry breaking''. Our result agree qualitatively with Monte Carlo simulations of three dimensional hard spheres.Comment: 7 pages, 7 figures, Revtex fil

Rationale and study design of the Prospective comparison of Angiotensin Receptor neprilysin inhibitor with Angiotensin receptor blocker MEasuring arterial sTiffness in the eldERly (PARAMETER) study.

Hypertension in elderly people is characterised by elevated systolic blood pressure (SBP) and increased pulse pressure (PP), which indicate large artery ageing and stiffness. LCZ696, a first-in-class angiotensin receptor neprilysin inhibitor (ARNI), is being developed to treat hypertension and heart failure. The Prospective comparison of Angiotensin Receptor neprilysin inhibitor with Angiotensin receptor blocker MEasuring arterial sTiffness in the eldERly (PARAMETER) study will assess the efficacy of LCZ696 versus olmesartan on aortic stiffness and central aortic haemodynamics

Metastable states in the Blume-Emery-Griffiths spin glass model

We study the Blume-Emery-Griffiths spin glass model in presence of an attractive coupling between real replicas, and evaluate the effective potential as a function of the density overlap. We find that there is a region, above the first order transition of the model, where metastable states with a large density overlap exist. The line where these metastable states appear should correspond to a purely dynamical transition, with a breaking of ergodicity. Differently from what happens in p-spin glasses, in this model the dynamical transition would not be the precursor of a 1-step RSB transition, but (probably) of a full RSB transition.Comment: RevTeX, 4 pages, 2 fig

Does cattle and sheep grazing under best management significantly elevate sediment losses? Evidence from the North Wyke Farm Platform, UK

Purpose Intensive livestock grazing has been associated with an increased risk of soil erosion and concomitant negative impacts on the ecological status of watercourses. Whilst various mitigation options are promoted for reducing livestock impacts, there is a paucity of data on the relationship between stocking rates and quantified sediment losses. This evidence gap means there is uncertainty regarding the cost-benefit of policy preferred best management. Methods Sediment yields from 15 hydrologically isolated field scale catchments on a heavily instrumented ruminant livestock farm in the south west UK were investigated over similar to 26 months spread across 6 years. Sediment yields were compared to cattle and sheep stocking rates on long-term, winter (November-April), and monthly timescales. The impacts of livestock on soil vegetation cover and bulk density were also examined. Cattle were tracked using GPS collars to determine how grazing related to soil damage. Results No observable impact of livestock stocking rates of 0.15-1.00 UK livestock units (LU) ha(-1) for sheep, and 0-0.77 LU ha(-1) for cattle on sediment yields was observed at any of the three timescales. Cattle preferentially spent time close to specific fences where soils were visually damaged. However, there was no indication that livestock have a significant effect on soil bulk density on a field scale

Does cattle and sheep grazing under best management significantly elevate sediment losses? Evidence from the North Wyke Farm Platform, UK

Purpose: Intensive livestock grazing has been associated with an increased risk of soil erosion and concomitant negative impacts on the ecological status of watercourses. Whilst various mitigation options are promoted for reducing livestock impacts, there is a paucity of data on the relationship between stocking rates and quantified sediment losses. This evidence gap means there is uncertainty regarding the cost–benefit of policy preferred best management. Methods: Sediment yields from 15 hydrologically-isolated field scale catchments on a heavily instrumented ruminant livestock farm in the south west UK were investigated over ~26 months spread across six years. Sediment yields were compared to cattle and sheep stocking rates on long-term, winter (November–April) and monthly time scales. The impacts of livestock on soil vegetation cover and bulk density were also examined. Cattle were tracked using GPS collars to determine how grazing related to soil damage. Results: No observable impact of livestock stocking rates of 0.15 – 1.00 UK livestock units (LU) ha-1 for sheep and 0 - 0.77 LU ha-1 for cattle on sediment yields was observed at any of the three timescales. Cattle preferentially spent time close to specific fences where soils were visually damaged. However, there was no indication that livestock have a significant effect on soil bulk density on a field-scale. Livestock were housed indoors during winters when most rainfall occurs and best management practices were used which when combined with low erodibility clayey soils likely limited sediment losses. Conclusion: A combination of clayey soils and soil trampling in only a small proportion of the field areas lead to little impact from grazing livestock. Within similar landscapes with best practice livestock grazing management, additional targeted measures to reduce erosion are unlikely to yield a significant cost-benefit

First steps of a nucleation theory in disordered systems

We devise a field theoretical formalism for a microscopic theory of nucleation processes and phase coexistence in finite dimensional glassy systems. We study disordered $p$-spin models with large but finite range of interaction. We work in the framework of glassy effective potential theory which in mean-field is a non-convex, two minima function of the overlap. We will associate metastability and phase coexistence with the existence of space inhomogeneous solution of suitable field equations and we will study the simplest of such solutions.Comment: 31 pages, 4 figures. Content revised, typos correcte