Bacterial cellulose-based hydrogel for wound healing: characterization and in vitro evaluation

Bacterial cellulose (BC) has been considered a promising biopolymer with applications in several areas of knowledge, including medicine, mainly due to its ability to assist in the treatment of dermal lesions. Many groups and companies have been making efforts to develop new BC-based materials in order to add new characteristics and therapeutic possibilities. Recently, Seven Indústria de Produtos Biotecnológicos Ltda company developed a BC-based hydrogel aiming to verify the interaction among the formulation components, its potential for wound healing and biocompatibility studies. BC-based hydrogel was characterized and compared with pristine BC film. Physicochemical characterization includes rheological measurements, thermal analyses, field emission gun - scanning electron microscopy (FE-SEM) and in vitro cell migration. BC-based hydrogel showed adequate interaction among the components of the formulation, which may positively influence its stability. In addition, the BC-based hydrogel accelerated the healing processes demonstrating its potential in dermal lesion treatment

Bacterial cellulose hydrogel and calcium alginate in the cicatricial wound process from diabetic foot implementation

Objectives: Due to the excellent properties of alginate-containing bacterial cellulose hydrogel, the objective was to treat a wound that is difficult to heal in a diabetic patient. Method: The treatment was carried out at the Santa Casa de Misericordia de São Carlos Ambulatory for 30 days, with the application of bacterial cellulose hydrogel containing alginate twice a week Results: After 30 days of treatment, the wound showed an 84% reduction in its initial size Conclusion: Bacterial cellulose hydrogel containing alginate has high potential for the treatment of wounds

Complexos de inclusão com amilose: obtenção, caracterização e avaliação como sistemas de liberação controlada de fármacos

High amylose, one modified starch with high content of amylose (70%) is a promising material for controlling the delivery of different drugs. The ability of the high amylose to form inclusion complexes with high enzymatic resistance was exploited for obtaining controlled drugs delivery systems for targeting the drug release in the intestine by digestion with pancreatin enzyme. Different complexes were produced and the influence of the ratio drug: polymer, temperature and the presence of palmitic acid were evaluated through the characterization of the complexes by DSC, X-ray diffraction, particle size distribution, NMR and liquid uptake analysis. The performance of these complexes as controlled drug delivery systems was evaluated by determining the in vitro release of nimesulide (NMS) and praziquantel (PZQ) in different media, simulating the different regions of the gastrointestinal (GI) tract. The methodology to obtain the complex has been successful with a yield over 57% and the content of drug complexation until 68.16%. The DSC, diffraction of X-rays and NMR data showed the interaction between drug and polymer as well as the formation of inclusion complexes with semi-crystalline structures which were related to the formation of type II complexes with high enzymatic resistance. The higher crystallinity and the presence of drugs near to the complexes surface promoted the reduction of liquid uptake ability. The inclusion of drugs in complex allowed the reduction of their rates of release in the acid media (pH 1.2) and phosphate buffer (pH 6.9). The addition of pancreatin enzyme caused a significant acceleration of the rate of release of drugs (NMS and PZQ) showing the degradability of these complexes. The higher crystallinity of PZQ1:30AG60°C complex showed its highest enzymatic resistance providing the release of PZQ during 240min, while the complexes with NMS and PZQ 1:5AG90°C complex released the drug in 60min. The release data in media...A alta amilose, um amido modificado com alto teor de amilose (70%), que demonstrou em diversos estudos ser um material promissor para o controle da liberação de diferentes fármacos. A habilidade da alta amilose em formar complexos de inclusão e a elevada resistência enzimática foi explorada para a obtenção de sistemas de liberação controlada de fármacos com intuito vetorizar a liberação do fármaco no intestino, através da digestão pela enzima pancreatina. Diferentes complexos foram produzidos e a influencia da proporção fármaco: polímero, temperatura, e a presença de ácido palmítico foram avaliados através da caracterização dos complexos pelas análises de DSC, difração de Raios-X, distribuição granulométrica, RMN e determinação da capacidade de absorção de líquido. O desempenho como sistemas de liberação controlada de fármacos foi avaliado através da determinação da liberação in vitro dos fármacos nimesulida (NMS) e praziquantel (PZQ) em diferentes meios, simulando as diferentes regiões do trato gastrintestinal (TGI). A metodologia para a obtenção dos complexos mostrou-se eficiente com rendimento superior a 57% e teor de fármaco complexado de até 68,16%. Os dados de DSC, difração de Raios-X e RMN relevaram a interação entre fármaco e polímero, bem como a formação dos complexos de inclusão com estruturas semi-cristalinas associadas a formação de complexos do tipo II, de elevada resistência enzimática. A maior cristalinidade e a presença de fármacos próximos à superfície dos complexos promoveram redução da capacidade de absorção de líquido. A inclusão dos fármacos nos complexos permitiu a redução de suas taxas de liberação nos meios sem enzima (ácido, pH 1,2 e tampão fosfato pH 6,9). A adição da enzima pancreatina promoveu significativa aceleração das taxas de liberação dos fármacos (NMS e PZQ) demostrando a degradabilidade desses complexos. A maior ...Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

The interaction between photonic technology and physical exercise: The action of low-level laser therapy

Introduction: Obesity is a global-level epidemic. Together with this disease, called chronic subclinical inflammatory disease, many other diseases, known as comorbidities, arise. Objective: To show that the association between low-level laser therapy and physical exercise is supported, by experimental and clinical studies, being an instrument that maximizes the treatment of obesity as well as its comorbidities. Conclusion: This manuscript brings a compendium of accomplished work by our group that allows understand the mechanism base of interaction between the photonic technology and the physical exercise, allowing to potentiate the treatment of the obesity

Correlação da gordura total do tronco e da gordura visceral em relação ao Índice de Massa Corporal de pacientes da Santa Casa de São Carlos-São Paulo

The present article aimed to delineate the anthropometric profile of men and women, evaluated by Bioelectrical Bioimpedance method, in the city of São Carlos, Brazil. A total of 438 individuals were evaluated, 132 males and 306 females. We obtained the analysis of the total body composition, analyzing the following variables: body weight; body mass index; total body fat; total trunk fat; visceral fat area; neck circumference; waist circumference; hip circumference, waist / hip ratio and basal metabolic rate. These data are important to diagnose predisposition to various diseases. The evaluation of body composition showed that exist a greater prevalence of people in the overweight and obesity ranges, in their different degrees, in relation to those that fit the parameter of normality. This prevalence was higher among men than among the women evaluated. It was also observed a higher concentration of visceral fat in men, indicating that the higher the visceral fat, the greater the total fat of the trunk and consequently, the greater the waist circumference. These indicators show the possibility of increased predisposition to the occurrence of various chronic-degenerative diseases associated with obesity.O presente estudo teve como objetivo delinear o perfil antropométrico de homens e mulheres, avaliado pelo método Bioimpedância Bioelétrica, na cidade de São Carlos, Brasil. Foram avaliados 438 indivíduos, sendo 132 do sexo masculino e 306 do sexo feminino. Obteve-se a análise da composição corporal total, analisando as seguintes variáveis: peso corporal; índice de massa corporal; Gordura corporal total; Gordura total do tronco; Área de gordura visceral; Circunferência do pescoço; circunferência da cintura; Circunferência do quadril, relação cintura / quadril e taxa metabólica basal. Estes dados são importantes para diagnosticar predisposição a várias doenças. A avaliação da composição corporal mostrou que existe uma maior prevalência de pessoas nas faixas de sobrepeso e obesidade, em seus diferentes graus, em relação às que se enquadram no parâmetro de normalidade. Essa prevalência foi maior entre os homens do que entre as mulheres avaliadas. Observou - se também maior concentração de gordura visceral nos homens, indicando que quanto maior a gordura visceral, maior a gordura total do tronco e consequentemente maior a circunferência da cintura. Esses indicadores mostram a possibilidade de maior predisposição à ocorrência de várias doenças crônico-degenerativas associadas à obesidade.

Pode a associação de Laser de baixa Intensidade e Treinamento Intervalado de Alta Intensidade (HIIT) melhorar a composição corporal de mulheres em condição de pós menopausa?

Obesity is an epidemic in this contemporary society. Non-pharmacological treatment standard takes into account the negative energy balance, which is the ratio of decreased caloric intake and increased energy expenditure. Exercise is the most effective strategy to promote the increase in total energy expenditure, which combined with phototherapy, is a very advantageous combination for the treatment of this disease. This study evaluates the low-intensity laser association with interval training high intensity in anthropometric improvement of postmenopausal women and their metabolic effect. We were selected 20 women aged from 50 to 55 years, body mass index between 30 and 34.9 kg/m2 , divided into 2 groups, described as a control group (exercise + nutritional education - no incidence of light) and treated group (exercise + nutritional education + incidence of light). The exercise protocol consisted of aerobic exercise and resistance, intense and interval (80%-85% of maximum heart rate), duration of 30 minutes, 3 times per week for 8 weeks. The phototherapy consisted of red LEDs, wavelength 660nm, potency 50mW, intensity 17,7mW/cm2, 10 minutes of treatment time, 30 irradiated points, totaling 318J; applied to the abdominal region after exercise. The proposed treatment was effective, improving all anthropometric parameters analyzed and condition as a new methodology for improving quality of life.A obesidade é uma epidemia presente na sociedade contemporânea. O tratamento padrão não farmacológico leva em conta o balanço energético negativo, o qual consiste na relação entre diminuição da ingestão calórica e aumento do gasto energético.  O exercício é a estratégia mais eficiente para promover o aumento do gasto energético total, o qual aliado à fototerapia, é uma conjugação muito vantajosa para o tratamento dessa doença. Este trabalho avalia a associação de laser de baixa intensidade ao treinamento intervalado de alta intensidade na melhora antropométrica de mulheres pós menopausa e seu efeito metabólico. Foram selecionadas 20 mulheres com faixa etária entre 50 a55 anos, índice de massa corporal entre 30 e 34,9 kg/m2, divididos em 2 grupos, descritos como grupo controle (exercício + reeducação alimentar - sem incidência de luz) e grupo tratado (exercício + reeducação alimentar + com incidência de luz). O protocolo de exercício foi composto de exercícios aeróbicos e resistidos, intervalados e intensos (80%-85% da frequência cardíaca máxima), duração de 30 minutos, 3 vezes por semana, por 8 semanas. A Fototerapia foi composta de LED vermelho, comprimento de onda de 660nm, potência média de 50mW, intensidade de 17,7mW/cm2, tempo de tratamento de 10 minutos, 30 pontos irradiados, totalizando 318J; aplicado na região abdominal, após exercício. O tratamento proposto mostrou ser eficaz, melhorando todos os parâmetros antropométricos analisados, condicionando-se como uma nova metodologia para a melhora da qualidade de vida

Eco-Friendly Extraction of Green Coffee Oil for Industrial Applications: Its Antioxidant, Cytotoxic, Clonogenic, and Wound Healing Properties

The development of natural oil-based cosmetic and pharmaceutical products presents great scientific and commercial interest. Herein, we aimed to extract green coffee oil from Arabic coffee by a sustainable cold-pressing method. Furthermore, this work aimed to characterize the obtained green coffee oil by Fourier-Transform Infrared (FT–IR) and ultraviolet–visible spectroscopies (UV–Vis), peroxide analysis, and fatty acids profile by gas chromatography–mass spectrometry (GC–MS). Moreover, the functional and biological properties of the obtained green coffee oil and a green-coffee oil-based commercial product (Energy up®, Dermociencia) were investigated. The green coffee oil presented linoleic and palmitic acids as the major fatty acids showing 44.8% and 35.4%, respectively. Moreover, this green coffee oil presented an antioxidant activity (EC50 7.64 mg/mL) and an absence of cytotoxic effects in keratinocyte cultures treated with up to 20 mg/mL. The obtained green coffee oil showed wound healing properties as well as clonogenic efficiency, a biological potential to induce the proliferative and migratory capacity of cells of human skin keratinocytes at 2.5 mg/mL. The samples presented high antioxidant activity and the absence of a cytotoxic effect, suggesting that green coffee oil is a promising natural product for cosmetic applications with wound healing properties. These results open new ways for the use of green coffee oil for the development of cosmetic and pharmaceutics natural-based products