Composite cellulose fibre for affinity chromatography application

Affinity chromatographic supports is nowadays one of the most used and expensive consumable material for protein purification at the laboratory and industrial scale. The introduction of cost-effective materials is an important issue to address to spread the usefulness of this technology. Cotton fibres are a highly available natural material with excellent mechanical and structural properties which can be used for this propose. Nevertheless, fibre insolubility and low chemical reactivity are the major drawbacks to use this material for protein chromatography.In this work, a composite material prepared from polymethacrylate/cellulose fibres were used for the preparation of chromatographic materials containing immobilised proteins as adsorptive ligands. A green fluorescent protein and a protein A simil ligand containing cysteine in the terminal tags were immobilised onto the fibres by the epoxy-thiol chemistry. Buffer salt, pH, reaction time and pre-swelling procedure were optimised. Materials were characterised by fluorescence and electron microscopes, in addition to the binding capacity of the adsorbent materials.Fil: Carbajal, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB; ArgentinaFil: Kikot, Pamela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB; ArgentinaFil: Torchio, Gabriela María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB; ArgentinaFil: Fernandez Lahore, M.. Universitat Bremen. School of Enigineerring and Science Jacobs; AlemaniaFil: Grasselli, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB; Argentin

An arsenic fluorescent compound as a novel probe to study arsenic-binding proteins

Arsenic-binding proteins are under continuous research. Their identification and the elucidation of arsenic/ protein interaction mechanisms are important because the biological effects of these complexes may be related not only to arsenic but also to the arsenic/protein structure. Although many proteins bearing a CXXC motif have been found to bind arsenic in vivo, new tools are necessary to identify new arsenic targets and allow research on protein/ arsenic complexes. In this work, we analyzed the performance of the fluorescent compound APAO-FITC (synthesized from p-aminophenylarsenoxide, APAO, and fluorescein isothiocyanate, FITC) in arsenic/protein binding assays using thioredoxin 1 (Trx) as an arsenic-binding protein model. The Trx-APAO-FITC complex was studied through different spectroscopic techniques involving UV-Vis, fluorescence, atomic absorption, infrared and circular dichroism. Our results show that APAO-FITC binds efficiently and specifically to the Trx binding site, labeling the protein fluorescently, without altering its structure and activity. In summary, we were able to study a protein/ arsenic complex model, using APAO-FITC as a labeling probe. The use of APAO-FITC in the identification of different protein and cell targets, as well as in in vivo biodistribution studies, conformational studies of arsenicbinding proteins, and studies for the design of drug delivery systems for arsenic anti-cancer therapies, is highly promising.Fil: Femia, Lis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Temprana, Carlos Facundo. Universidad Nacional de Quilmes; ArgentinaFil: Santos, Javier. Universidad de Buenos Aires; ArgentinaFil: Carbajal, Maria Laura. Universidad Nacional de Quilmes; ArgentinaFil: Amor, María Silvia. Universidad Nacional de Quilmes; ArgentinaFil: Grasselli, Mariano. Universidad Nacional de Quilmes; ArgentinaFil: Alonso, Silvia del Valle. Universidad Nacional de Quilmes; Argentin

Emerging technologies for the integration and intensification of downstream bioprocesses

Downstream processing is currently the major bottleneck for bioproduct generation. In contrast to the advances in fermentation processes, the tools used for downstream processes have struggled to keep pace in the last 20 years. Purification bottlenecks are quite serious, as these processes can account for up to 80% of the total production cost. Coupled with the emergence of new classes of bioproducts, for example, virus-like particles or plasmidic DNA, this has created a great need for superior alternatives. In this review, improved downstream technologies, including aqueous two-phase systems, expanded bed adsorption chromatography, convective flow systems, and fibre-based adsorbent systems, have been discussed. These adaptive methods are more suited to the burgeoning downstream processing needs of the future, enabling the cost-efficient production of new classes biomaterials with a high degree of purity, and thereby hold the promise to become indispensable tools in the pharmaceutical and food industries.Fil: D’Souza, Roy N.. Universitat Bremen; AlemaniaFil: Azevedo, Ana M.. Universidad de Lisboa; PortugalFil: Aires Barros, M. Raquel. Universidad de Lisboa; PortugalFil: Krajnc, Nika Lendero. BIA Separations; EsloveniaFil: Kramberger, Petra. BIA Separations; EsloveniaFil: Carbajal, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Grasselli, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Meyer, Ronald. Universitat Bremen; AlemaniaFil: Fernandez Lahore, Marcelo. Universitat Bremen; Alemani

Análisis geográfico, ambiental y ecológico del Cerro de Coatepec Universidad Autónoma del Estado de México

El libro “Los espacios universitarios como objeto de estudio. Análisis geográfico, ambiental y ecológico del Cerro de Coatepec, Universidad Autónoma del Estado de México”, es resultado de la participación de un grupo multidisciplinario integrado por investigadores, profesores y estudiantes de la Universidad Autónoma del Estado de México, así como del grupo de asesores y consultores del Colegio de Ciencias Geográficas del Estado de México, A. C. (COCIGEM), interesados en la problemática ambiental y ecológica que ocurre en la Ciudad Universitaria ubicada geográficamente en el Cerro de Coatepec, Toluca Estado de México, en el cual se encuentran establecidas algunas edificaciones de dependencias de educación superior, centros e institutos de investigación, difusión cultural, dependencias administrativas, bibliotecas, museos e instalaciones deportivas.Los ejes rectores de análisis contenidos en este volumen son siete: a) la caracterización geográfica, ecológica, ambiental, sociocultural e infraestructural del espacio que comprende el Cerro de Coatepec; b) el análisis del Cerro de Coatepec como un ecosistema urbano en el contexto de la Zona Metropolitana del Valle de Toluca, Estado de México; c) la multifuncionalidad de las áreas verdes, las áreas arboladas y los espacios naturales del ecosistema; d) los impactos ambientales que han ocasionado y que ocasionan las etapas de preparación del sitio, la construcción de edificaciones y la ejecución de actividades docentes, de investigación, difusión, deportivas y socioculturales; e) los riesgos que ocurren y pueden ocurrir en las áreas verdes, las áreas arboladas, los ambientes naturales y la infraestructura de las edificaciones; f) la responsabilidad ambiental e importancia de la participación de los actores universitaritos en el cuidado del entorno universitario; y g) propuesta de acciones generales para el mejoramiento de las condiciones del paisaje y manejo del entorno universitario. Estos ejes rectores no se analizan de manera independiente, sino en una dimensión integral, ya que la alteración de algún componente del ambiente o la existencia de riesgos puede tener diferentes causas y por consiguiente diversos efectos.Universidad Autónoma del Estado de México Colegio de Ciencias Geográficas del Estado de México AC

Impact of common cardio-metabolic risk factors on fatal and non-fatal cardiovascular disease in Latin America and the Caribbean: an individual-level pooled analysis of 31 cohort studies

Background: Estimates of the burden of cardio-metabolic risk factors in Latin America and the Caribbean (LAC) rely on relative risks (RRs) from non-LAC countries. Whether these RRs apply to LAC remains un- known. Methods: We pooled LAC cohorts. We estimated RRs per unit of exposure to body mass index (BMI), systolic blood pressure (SBP), fasting plasma glucose (FPG), total cholesterol (TC) and non-HDL cholesterol on fatal (31 cohorts, n = 168,287) and non-fatal (13 cohorts, n = 27,554) cardiovascular diseases, adjusting for regression dilution bias. We used these RRs and national data on mean risk factor levels to estimate the number of cardiovascular deaths attributable to non-optimal levels of each risk factor. Results: Our RRs for SBP, FPG and TC were like those observed in cohorts conducted in high-income countries; however, for BMI, our RRs were consistently smaller in people below 75 years of age. Across risk factors, we observed smaller RRs among older ages. Non-optimal SBP was responsible for the largest number of attributable cardiovascular deaths ranging from 38 per 10 0,0 0 0 women and 54 men in Peru, to 261 (Dominica, women) and 282 (Guyana, men). For non-HDL cholesterol, the lowest attributable rate was for women in Peru (21) and men in Guatemala (25), and the largest in men (158) and women (142) from Guyana. Interpretation: RRs for BMI from studies conducted in high-income countries may overestimate disease burden metrics in LAC; conversely, RRs for SBP, FPG and TC from LAC cohorts are similar to those esti- mated from cohorts in high-income countries

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Are gender biases reproduced in current biotechnology learning? Study case: classroom of “protein recovery and purification” of the Bachelor of Biotechnology at Quilmes National University

A partir de producciones escritas de dos cohortes, y mediante el análisis discursivo cualitativo, se pretende visibilizar y diagnosticar en qué medida el profesorado y el estudiantado de esta asignatura mantienen concepciones, supuestos, asunciones y valores sesgados acerca del género. Se asume la hipótesis de que existen sesgos de género en las ciencias (investigaciones y teorías) y que se transmiten en la educación y formación científica, perpetuándose. En un primer estudio, se propone analizar con perspectiva de género el material didáctico generado por el cuerpo docente, desde el punto de vista del lenguaje (sexuado o inclusive) y los modelos de ciencia que se transmiten en las representaciones semióticas. Luego, se propone analizar, por medio de dimensiones psicosociales, la producción escrita de las personas estudiantes (en particular las reflexiones sobre la práctica), en la búsqueda de creencias, expectativas y valoraciones. Y por último, también en las reflexiones sobre la práctica, se busca comparar las autopercepciones sobre el rendimiento académico. A partir del análisis cualitativo propuesto, se detectaron sesgos de género en el aula de “Recuperación y Purificación de Proteínas”, de la carrera de biotecnología de la UNQ, validándose la hipótesis propuesta. Por lo cual, se proponen diferentes acciones para promover la equidad de género a través de la comunicación en el aula.The aim of this work is to make visible and diagnose to what extent the teachers and students of this subject maintain gender biased conceptions, assumptions and values. The hypothesis assumed it that there are gender biases in the sciences and that they are transmitted and perpetuated in scientific education. In goal to achieve it, written productions of two cohorts were analyzed it through qualitative discursive analysis. Then, it was proposed to analyze, by means of psychosocial dimensions, the written production of the students (in particular the reflections on the practice), in the search for beliefs and expectations. In addition, the didactic material generated by the teachers, were also analyzed it from a gender perspective. It was focus on the point of view of language (sexed or inclusive) and on which model of science was transmitted it in semiotic representations. Finally, on student’s reflections on own practice, it seek to compare self-perceptions on academic performance. Based on the proposed qualitative analysis, gender biases were detected it at the “Protein Recovery and Purification” classroom of the UNQ biotechnology career, validating the proposed hypothesis. Therefore, different actions were proposed to promote gender equality through communication in the classroom.Fil: Carbajal, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencias Sociales. Instituto de Estudios Sociales de la Ciencia y la Tecnología; Argentin

Bimodalidad en la asignatura Recuperación y Purificación de Proteínas

La asignatura Recuperación y Purificación de Proteínas (RPP) pertenece al núcleo electivo de la Licenciatura en Biotecnología de la UNQ, en el plan de estudios 2011 y es de carácter obligatoria en el plan 2018. Se encuadra dentro del área de conocimiento de la Biotecnología Industrial y los Bioprocesos. Algunos contenidos que se desarrollan en RPP son: 1) Introducción al dowstream processing; 2) Ruptura celular; 3) Separaciones sólido-líquido (filtración, sedimentación y centrifugación); 4) Precipitación; 5) Partición en dos fases acuosas; 6) Cromatografías no adsortivas y adsortivas; 7) Cromatografía preparativa; 8) Cromatografía convectiva; 9) Diseño y optimización de procesos de purificación. Dentro de las asignaturas previas necesarias para favorecer el aprendizaje se encuentran Bioquímica I y Diseño Experimental (o Estadística y Probabilidad). Para su acreditación se contemplan distintos instrumentos de evaluación: diagnóstica, de proceso y final o integradora. El objetivo de RPP es que el estudiante comprenda las diferentes etapas de recuperación y purificación que involucran la obtención de un producto biotecnológico (downstream). Así como también, el análisis y la comparación de las diferentes tecnologías para cada operación unitaria, para introducir en la problemática de la escala piloto-industrial...Fil: Carbajal, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencias Sociales. Instituto de Estudios Sociales de la Ciencia y la Tecnología; Argentin

The eco-epidemiology of Triatoma infestans in the temperate monte desert ecoregion of mid-western Argentina

BACKGROUND The eco-epidemiological status of Chagas disease in the Monte Desert ecoregion of western Argentina is largely unknown. We investigated the environmental and socio-demographic determinants of house infestation with Triatoma infestans, bug abundance, vector infection with Trypanosoma cruzi and host-feeding sources in a well-defined rural area of Lavalle Department in the Mendoza province. METHODS Technical personnel inspected 198 houses for evidence of infestation with T. infestans, and the 76 houses included in the current study were re-inspected. In parallel with the vector survey, an environmental and socio-demographic survey was also conducted. Univariate risk factor analysis for domiciliary infestation was carried out using Firth penalised logistic regression. We fitted generalised linear models for house infestation and bug abundance. Blood meals were tested with a direct ELISA assay, and T. cruzi infection was determined using a hot-start polymerase chain reaction (PCR) targeting the kinetoplast minicircle (kDNA-PCR). FINDINGS The households studied included an aged population living in precarious houses whose main economic activities included goat husbandry. T. infestans was found in 21.2% of 198 houses and in 55.3% of the 76 re-inspected houses. Peridomestic habitats exhibited higher infestation rates and bug abundances than did domiciles, and goat corrals showed high levels of infestation. The main host-feeding sources were goats. Vector infection was present in 10.2% of domiciles and 3.2% of peridomiciles. Generalised linear models showed that peridomestic infestation was positively and significantly associated with the presence of mud walls and the abundance of chickens and goats, and bug abundance increased with the number of all hosts except rabbits. MAIN CONCLUSIONS We highlight the relative importance of specific peridomestic structures (i.e., goat corrals and chicken coops) associated with construction materials and host abundance as sources of persistent bug infestation driving domestic colonisation. Environmental management strategies framed in a community-based programme combined with improved insecticide spraying and sustained vector surveillance are needed to effectively suppress local T. infestans populations.Fil: Carbajal de la Fuente, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Provecho, Yael Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Fernandez, Maria del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Cardinal, Marta Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Lencina, Patricia. Ministerio de Salud de la Nación; ArgentinaFil: Spillmann, Cynthia. Ministerio de Salud de la Nación; ArgentinaFil: Gurtler, Ricardo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentin

Morphometric Wings Similarity among Sylvatic and Domestic Populations of Triatoma infestans (Hemiptera: Reduviidae) from the Gran Chaco Region of Paraguay

Despite sustained efforts for eliminating Triatoma infestans, reinfestation still persists in large part of the endemic area of Chagas disease from the Gran Chaco region. Sylvatic T. infestans populations seem to threat success of control programs of domestic T. infestans. In this study, we analyzewhether T. infestans collected after a community-wide spraying were survivors or were immigrants from elsewhere using geometric morphometric tools. We used 101 right wings of female T. infestans captured before and after intervention program carried out in 12 de Junio and Casuarina, villages from Paraguayan Chaco, and in Puerto Casado during presprayed collection. There were no significant differences in wing size of domestic T. infestans between pre- and postspraying populations, and between domestic and sylvatic ones.When shape variables originating from postintervention individuals from 12 de Juniowere introduced one by one into a discriminant analysis, the greatest weight (53%)was allocated to the sylvatic group. Furthermore, from the prespraying population, 25% were reallocated as postintervention individuals. Only 11% of the insects were reassigned to other groups Puerto Casado and Casuarina. These results suggest that postspraying individuals appear to have different origins. Half of the postspraying individuals from 12 de Junio were similar to the sylvatic ones and 25% of these were similar to those captured in the prespraying period. This remarkable morphometric wings similarity between sylvatic and domestic populations is new evidence suggesting that they could be highly related to each other in the Paraguayan Chaco; human-fed bugs from sylvatic area also support this.Fil: Rojas De Arias, Antonieta. Fundación Moisés Bertoni; ParaguayFil: Carbajal de la Fuente, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Gómez, Ana. Fundación Moisés Bertoni; ParaguayFil: Cecere, Maria Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Rolón, Miriam. Fundación Moisés Bertoni; ParaguayFil: Vega Gómez, María Celeste. Fundación Moisés Bertoni; ParaguayFil: Villalba, Cesia. Programa Nacional de Control de la Enfermedad de Chagas; Paragua