Randomized controlled trials and real-world data : differences and similarities to untangle literature data

Randomized controlled trials (RCTs) represent the gold-standard of medical evidence to assess the efficacy and safety of therapeutic interventions. However, the need to minimize bias and ensure the correct design to explore the study aims often affects the generalizability of results. As a consequence, the evidence derived from the most rigorous research strategy available is not always representative of the real-world settings for which this evidence is ultimately intended. Observational studies, in contrast, although affected by a number of potential confounders, can more effectively capture treatment characteristics and safety issues that had not been identified by previous RCTs, owing to the short duration of follow-up or highly selective inclusion criteria. The aim of this review is to provide a comparative summary of the main advantages and pitfalls of RCTs and real-world data, emphasizing the need for a constant integration of all available levels of evidence to provide the best care for patients

Occurrence probability of moderate to large earthquakes in Italy based on new geophysical methods

We develop new approaches to calculating 30-year probabilities for occurrence of moderate-to-large earthquakes in Italy. Geodetic techniques and finite-element modelling, aimed to reproduce a large amount of neotectonic data using thin-shell finite element, are used to separately calculate the expected seismicity rates inside seismogenic areas (polygons containing mapped faults and/or suspected or modelled faults). Thirty-year earthquake probabilities obtained from the two approaches show similarities in most of Italy: the largest probabilities are found in the southern Apennines, where they reach values between 10% and 20% for earthquakes of M W ≥ 6.0, and lower than 10% for events with an M W ≥ 6.5

SERS, XPS and DFT study of xanthine adsorbed on citrate-stabilized gold nanoparticles

We have studied the adsorption of xanthine, a nucleobase present in human tissue and fluids that is involved in important metabolic processes, on citrate-reduced gold colloidal nanoparticles by means of surface-enhanced Raman scattering (SERS), absorption, and X-ray photoelectron spectroscopy (XPS) measurements, along with density functional theory (DFT) calculations. The citrate anions stabilize the colloidal suspensions by strongly binding the gold nanoparticles. However, these anions do not impair the adsorption of xanthine on positively-charged active sites present on the metal surface. We have obtained the Fourier transform (FT)-SERS spectra of adsorbed xanthine by laser excitation in the near infrared spectral region, where interference due to fluorescence emission does not usually occur. In fact, the addition of chloride ions to the Au/xanthine colloid induces the aggregation of the gold nanoparticles, whose plasmonic band is shifted to the near infrared region where there is the exciting laser line of the FT\u2013Raman instrument. Hence, this analytical approach is potentially suitable for spectroscopic determination of xanthine directly in body fluids, avoiding fluorescence phenomena induced by visible laser irradiation

Legionnaires' disease in Italy: results of the epidemiological surveillance from 2000 to 2011

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