Maintaining Glucose Homeostasis in Response to Aging and Stress: The Role of Pcif1, Bmi1, and Pdx1

A sufficient number of functioning beta cells is necessary for maintaining glucose homeostasis. Reduction of beta cell mass or function leads to diabetes. Investigation into the maintenance of both beta cell mass and function is important for the development of therapies to prevent and/or restore functional beta cells. Here, the networks surrounding three proteins in the beta cell, Pcif1, Bmi1, and Pdx1, were studied as they relate to beta cell function and number. The Polycomb protein, Bmi1, has been shown to influence beta cell replication via epigenetic repression of the Ink4a/Arf locus, resulting in suppression of p16 protein translation. The adapter protein, Pcif1, facilitates the ubiquitination of Bmi1 and influences beta cell replication, as Pcif1 heterogyzous mice have increased rates of beta cell proliferation. I hypothesized that Pcif1 regulates beta cell proliferation through a Bmi1-dependent mechanism. Analysis of Pcif1 heterozygous islets revealed that p16 protein levels were indistinguishable from controls, thus making a p16-dependent mechanism unlikely. Further investigation of Bmi1 targets may reveal another pathway by which Pcif1 and Bmi1 influence beta cell replication. The role of Bmi1 has not been well-described in adult animals. Analysis of Bmi1 heterozygous animals revealed increased insulin sensitivity, as compared to wildtype. This was found to be due to an enhancement of Akt phosphorylation, with the upstream insulin signaling pathway unaffected. Bmi1 also appears to play a role in the development of insulin resistance, as Bmi1 levels are high in insulin-resistant animals. I also began to explore the possibility that the action of Pcif1 on Bmi1 is responsible for the role Bmi1 plays in insulin signaling. The transcription factor, Pdx1, regulates numerous processes specific to the beta cell, including multiple pathways regulating translation. Pdx1 levels have been shown to affect the ability of beta cells to respond to ER stress. A global analysis of translational efficiencies using the TRAP methodology indicated that Pdx1 activity may result in repression of translation of some transcripts. Further analysis of these transcripts will help determine how Pdx1 regulates the translatome of the beta cell and, potentially, how Pdx1 influences the beta cell stress response

Strategies for Improving Data Protection to Reduce Data Loss from Cyberattacks

Accidental and targeted data breaches threaten sustainable business practices and personal privacy, exposing all types of businesses to increased data loss and financial impacts. This single case study was conducted in a medium-sized enterprise located in Brevard County, Florida, to explore the successful data protection strategies employed by the information system and information technology business leaders. Actor-network theory was the conceptual framework for the study with a graphical syntax to model data protection strategies. Data were collected from semistructured interviews of 3 business leaders, archival documents, and field notes. Data were analyzed using thematic, analytic, and software analysis, and methodological triangulation. Three themes materialized from the data analyses: people--inferring security personnel, network engineers, system engineers, and qualified personnel to know how to monitor data; processes--inferring the activities required to protect data from data loss; and technology--inferring scientific knowledge used by people to protect data from data loss. The findings are indicative of successful application of data protection strategies and may be modeled to assess vulnerabilities from technical and nontechnical threats impacting risk and loss of sensitive data. The implications of this study for positive social change include the potential to alter attitudes toward data protection, creating a better environment for people to live and work; reduce recovery costs resulting from Internet crimes, improving social well-being; and enhance methods for the protection of sensitive, proprietary, and personally identifiable information, which advances the privacy rights for society

The science of bridging differences and the dialogic transformation of conflict: a case study from «This is Us»

In a climate of increasing social and political polarisation, there is much interest in dialogue and bridging differences. This article investigates the dramatisation of dialogic conflict resolution, examining how the psychological science of bridging differences can be utilised to create realistic and emotionally engaging narratives that highlight the transformative impact of dialogue on character development and relational dynamics. Relational conflicts have long been recognised as possible catalysts for character transformation, and complex narratives (Mittell, 2015) are particularly appropriate for a nuanced representation of characters’ psychological experiences throughout the process. This study draws upon extensive psychological and communication research on bridging differences compiled by Shigeoka et al. (2020) and UC Berkeley’s Greater Good Science Center (2021). It reorganises this research into a horizontal timeline of behaviours, highlighting their practical application during the conflict resolution process. It then makes a cognitive poetics analysis of how these behaviours may be observed in a case study of relational conflict from the complex television series This is Us (NBC, 2016-2022). By observing the impact these behaviours have on the transformation of characters, the study explores how credible dialogic relational conflicts can lead to verisimilar character transformation on screen. In addition, it considers how an artistic interpretation of the science of bridging differences can contribute to our understanding of dialogue in practice

A Study Abroad Program in Tanzania: The Evolution of Social Justice Action Work

This article focuses on the evolution of our study abroad program to Tanzania, including the integration of three liberal arts disciplines and the foundational core that links together the three areas: social justice activism. More specifically, we explore the overall challenge of dismantling “us versus them” thinking and the interactive learning moments that allow this process to transpire. We narrate how what we learned on our 2008 and our 2010 trips led to our model of social justice action work, which we enacted on our 2012 trip. Our approach to social justice action work integrates experiential learning with Dan Butin’s concept of “justice learning,” or education that interrupts and complicates binary thinking. Our three-fold model encompasses teaching moments where instructors create the academic framework to facilitate change in our students, where students observe grass-roots organizations performing “traditional” social justice action work, and where on-site activities generate interactive experiential moments in which perceptions can be changed

Identifying galaxy candidates in WSRT HI imaging of ultra-compact high velocity clouds

Ultra-compact high velocity clouds (UCHVCs) were identified in the ALFALFA HI survey as potential gas-bearing dark matter halos. Here we present higher resolution neutral hydrogen (HI) observations of twelve UCHVCS with the Westerbork Synthesis Radio Telescope (WSRT). The UCHVCs were selected based on a combination of size, isolation, large recessional velocity and high column density as the best candidate dark matter halos. The WSRT data were tapered to image the UCHVCs at 210" (comparable to Arecibo) and 105" angular resolution. In a comparison of the single-dish to interferometer data, we find that the line flux recovered in the WSRT observations is comparable to that from the single-dish ALFALFA data. In addition, any structure seen in the ALFALFA data is reproduced in the WSRT maps at the same angular resolution. At 210'" resolution all the sources are generally compact with a smooth HI morphology, as expected from their identification as UCHVCs. At the higher angular resolution, a majority of the sources break into small clumps contained in a diffuse envelope. These UCHVCs also have no ordered velocity motion and are most likely Galactic halo clouds. We identify two UCHVCs, AGC 198606 and AGC 249525, as excellent galaxy candidates based on maintaining a smooth HI morphology at higher angular resolution and showing ordered velocity motion consistent with rotation. A third source, AGC 249565, lies between these two populations in properties and is a possible galaxy candidate. If interpreted as gas-bearing dark matter halos, the three candidate galaxies have rotation velocities of 8-15 km/s, HI masses of 0.6-50 x 10^5 Msun, HI radii of 0.3-2 kpc, and dynamical masses of 2-20 x 10^7 Msun for a range of plausible distances. These are the UCHVCs with the highest column density values in the ALFALFA HI data and we suggest this is the best way to identify further candidates.Comment: Accepted for publication in A&A. 26 pages, 19 figures, 5 table

Detection of an Optical Counterpart to the ALFALFA Ultra-compact High Velocity Cloud AGC 249525

We report on the detection at $>$98% confidence of an optical counterpart to AGC 249525, an Ultra-Compact High Velocity Cloud (UCHVC) discovered by the ALFALFA blind neutral hydrogen survey. UCHVCs are compact, isolated HI clouds with properties consistent with their being nearby low-mass galaxies, but without identified counterparts in extant optical surveys. Analysis of the resolved stellar sources in deep $g$- and $i$-band imaging from the WIYN pODI camera reveals a clustering of possible Red Giant Branch stars associated with AGC 249525 at a distance of 1.64$\pm$0.45 Mpc. Matching our optical detection with the HI synthesis map of AGC 249525 from Adams et al. (2016) shows that the stellar overdensity is exactly coincident with the highest-density HI contour from that study. Combining our optical photometry and the HI properties of this object yields an absolute magnitude of $-7.1 \leq M_V \leq -4.5$, a stellar mass between $2.2\pm0.6\times10^4 M_{\odot}$ and $3.6\pm1.0\times10^5 M_{\odot}$, and an HI to stellar mass ratio between 9 and 144. This object has stellar properties within the observed range of gas-poor Ultra-Faint Dwarfs in the Local Group, but is gas-dominated.Comment: 9 pages, 4 figures; accepted to ApJ

The Atlántida of Capitalism. The murals of Sert in the decorative programme of New York’s Rockefeller Center

New York’s Rockefeller Center is one of most symbolically rich places in the world, although few of its millions of visitors stop to reflect on what its images of power really mean. In the form of an Atlantean mythological allegory, Rockefeller Center was conceived as symbolic propaganda for capitalist, liberal values implicit in both the ‘American Dream’ and the ideology espoused by the Rockefeller family. It embodies the utopia of progress and science that promotes the freedom of the individual and the free movement of capital. Due to ideological clashes –or the vagaries of fate– the Catalan José María Sert was the artist to ultimately complete the most eloquent mural in the main building, a mural which had formerly been painted by Diego de Rivera, and entitled Man at the Crossroads. Sert was a muralist who had previously worked on the scenographic illustration of Manuel de Falla’s Atlántida, capturing some of the motifs that inspired that great cantata based on poetic texts by Jacint Verdaguer. That earlier work is reflected in the lobby of Rockefeller Center’s main building. While Diego de Rivera’s censored frescoes have been studied prolifically, little attention has been paid to Sert’s paradoxical reading of the same subjects. In this article, we analyse the history of the Atlantean Mediterranean literary myth in relation to Spain, the use John D. Rockefeller Jr. made of them in his emblematic urbanistic ensemble, and also the peculiar reading that the Catalan muralist made of these themes of Atlantis in relation to capitalism