Single versus double experimental bile duct ligation model for inducing bacterial translocation

Background: Double common bile duct ligation plus section in rats is used as a model for bacterial translocation, a phenomenon that has been correlated with the degree of liver damage. This study analyzes whether a simpler variant of the technique is also a valid model to study bacterial translocation. Methods: Fifty-six male Sprague Dawley rats underwent one of three surgical interventions: a) proximal double ligation and section of the common bile duct; b) proximal simple ligation of the bile duct; and c) sham operation. Bacterial translocation was measured by cultures of mesenteric lymph nodes, blood, spleen and liver. Stool culture and histological analysis of liver damage were also performed. Results: The incidence of bacterial translocation in SBL and DBDL groups was 23,5% and 25% respectively. Mortality was similar between ligation groups (11.2% versus 10%). Liver cirrhosis developed in the group of double ligation and section (100% of the animals at 4 weeks), while portal hypertension appeared starting at week 3. None of the animals submitted to simple ligation developed liver cirrhosis. Conclusions: Simple bile duct ligation is associated with a similar incidence of bacterial translocation as double ligation, but without cirrhosis or portal hypertension

Mediterranean Sea level and barotropic flow through the Strait of Gibraltar for the period 1958-2001 and reconstructed since 1659

Sea level values from a two-dimensional model of the Mediterranean Sea forced by atmospheric pressure and wind are used to estimate the barotropic flow through the Strait of Gibraltar for the period 1958–2001. The Mediterranean mean sea level derived from the model ranges between ±20 cm with a standard deviation of 5.5 cm and is correlated to the North Atlantic Oscillation (NAO) index. Thus NAO historical data and reconstructions are used to derive the Mediterranean Sea level variability from 1659 until 2001. The accuracy of the reconstruction is estimated in 2.7 cm for monthly mean values, 0.41 cm for annual mean values, and 0.22 cm for decadal mean values (0.48 cm for decadal winter mean sea level). The barotropic flow through the strait is computed from the model output as the time derivative of the total volume of the basin. During the period 1958–2001 the estimated daily flow ranges between ±2.7 Sv, with a standard deviation of 0.56 Sv. The dominant periodicities are in between 1 and 2 weeks. At these scales the model successfully reproduces previously published flow estimates based on current meter observations, which confirms that atmospheric pressure and wind dominate the intraseasonal variability of the flow. For the annual cycle the variability of the atmospherically induced flow is similar to the variability of the flow induced by the evaporation-precipitation (E-P) budget (±0.025 Sv), though absolute values of the first are about a third of the latter. At longer timescales the atmospheric contribution is much smaller than the E-P induced flow

Meteotsunami (\u201cMarrobbio\u201d) of 25\u201326 June 2014 on the Southwestern Coast of Sicily, Italy

A major tsunami-like event, locally known as \u2018marrobbio\u2019, impacted the southwestern coast of Sicily on 25\u201326 June 2014. The event was part of a chain of hazardous episodes in the Mediterranean and Black seas during the last week of June 2014 resulting from an anomalous atmospheric system (\u201ctumultuous atmosphere\u201d) propagating eastward over the region. The synoptic patterns and vertical structure of the atmosphere over Sicily at the time of the event indicate that atmospheric wave ducting was responsible for the generation of tsunamigenic air pressure disturbances that produced especially high sea level responses (\u201cmeteotsunamis\u201d) at certain sites along the Sicilian coast. The strongest sea level oscillations were observed at Mazara del Vallo, where a 1-m meteotsunami bore, propagating upstream in the Mazaro River, was generated. The combined effects of external resonance (Proudman resonance on the western Sicilian shelf) and internal resonant conditions (bathymetric and topographic characteristics of specific sites) were found to be the key factors that caused the meteotsunami (marrobbio phenomenon) on the coast of Sicily and the meteobore at Mazara del Vallo

Meteorological tsunamis on the US East Coast and in other regions of the World Ocean

The TMEWS (Towards a meteotsunami warning system along the US coastline, http://jadran.izor.hr/tmews) project was funded by NOAA, Award No. NA11NWS4670005. Work on this issue by Ivica Vilibic is supported by Ministry of Science, Education and Sports of the Republic of Croatia and by Alexander B. Rabinovich is partly supported by RFBR Grants 12-05-00733-a and 12-05-00757-aPeer Reviewe