ANTI-OXIDANT EFFECTS OF POMEGRANATE JUICE ON SACCHAROMYCES CEREVISIAE CELL GROWTH

Background: Pomegranate juice has a number of positive effects on both human and animal subjects. Material and methods: Four groups were used in this study. i: Control group, ii: H 2 O 2 group, iii: Pomegranate juice (PJ) group and iv: PJ + H 2 O 2 group. Following the sterilization method for pomegranate juice (10%) and H 2 O 2 (6% v/v), Saccharomyces cerevisiae cultures were added and the cultivation incubated at 35°C for 72 hours. Fatty acids and vitamin concentrations were measured using HPLC and GC and the total protein bands profile were determined by SDS-PAGE. Results: According to our results statistically significant differences have been determined among the study groups in terms of fatty acids and vitamin (

Nigella sativa improves the carbon tetrachloride-induced lung damage in rats through repression of erk/akt pathway

Can, Muhammed İsmail (Aksaray, Yazar)The objective of this study was to examine whether Nigella sativa plays a protective role against the damage in the lung by administering carbon tetra-chloride (CCl4) to rats. Male Wistar albino (n=28, 8 weeks old) rats were divided into 4 groups: a) negative control: Normal water consuming group to which no CCl4 and N. sativa was administered; b) Positive control: Normal water consuming group to which no CCl4 was administered but N. sativa was administered; c) CCl4 Group: Normal water consuming and group to which CCl4 was administered (1.5 mL/kg, ip); d) N. sativa plus CCl4group: CCl4and N. sativa administered group (1.5 mL/kg, ip). Caspase-3, caspase -9, erk, akt protein syntheses were examined via Western blotting. Malondialdehyde determination in lung tissue was made using spectrophotometer. As a results, malondialdehyde amount was decreased in the CCl4 plus N. sativa group in comparison to CCl4 group whereas caspase-3, caspase-9 was increased and erk, akt had decreased. These results show that N. sativa protects the lung against oxidative damage

Black cumin may be a potential drug for development of carbontetrachloride-induced lung damage in rats

The study examines whether Black cumin (Nigella sativa L.) plays a protective role against the damage in the lung by administering carbontetrachloride (CCl4) to rats. 28 male Wistar albino (n=28, 8 weeks old) rats were used in the study. The rats divided into 4 groups according to their live weights. The groups were: (i) Negative Control (NC): Normal water consuming group to which no CCl4 and Black cumin (BC) is administered; (ii) Positive Control (PC): Normal water consuming group to which no CCl4 is administered but BC is administered; (iii) CCl4 Group: Normal water consuming and group to which CCl4 is administered (1.5 ml/kg live weight, ip); (iv) CCl4 + BC group: CCl4 and BC administered group (1.5 ml/kg live weight, ip). Tissue apoptotic index was examined via TUNEL method. MDA (malondialdehyde) determination in lung tissue was made using spectrophotometer. As a results, MDA amount decreased in the CCl4 + BC group (6,33 ± 1,54 nmol/g) in comparison to CCl4 group (8,66 ± 1,58 nmol/g) whereas it was observed in the CCl4+BC group (15,35 ± 0,21%) that the apoptotic index (TUNEL results) decreases in comparison with the CCl4 group (27,48 ± 0,28%) thus approaching normal values. DNA damage ratio decreased in the CCl4 + BC group in comparison to CCl4 group. These results show that BC plant protects the lung against oxidative damage. © Mattioli 1885

Comparison of dialysates with and without glucose in hemodialysis patients

44th ERA-EDTA Congress -- JUN 22-24, 2007 -- Barcelona, SPAINWOS: 000253320601031ERA-EDT

Mortality reduction by post-dilution online-haemodiafiltration : A cause-specific analysis

Background. From an individual participant data (IPD) meta-analysis from four randomized controlled trials comparing haemodialysis (HD) with post-dilution online-haemodiafiltration (ol-HDF), previously it appeared that HDF decreases all-cause mortality by 14% (95% confidence interval 25; 1) and fatal cardiovascular disease (CVD) by 23% (39; 3). Significant differences were not found for fatal infections and sudden death. So far, it is unclear, however, whether the reduced mortality risk of HDF is only due to a decrease in CVD events and if so, which CVD in particular is prevented, if compared with HD. Methods. The IPD base was used for the present study. Hazard ratios and 95% confidence intervals for cause-specific mortality overall and in thirds of the convection volume were calculated using the Cox proportional hazard regression models. Annualized mortality and numbers needed to treat (NNT) were calculated as well. Results. Besides 554 patients dying from CVD, fatal infections and sudden death, 215 participants died from 'other causes', such as withdrawal from treatment and malignancies. In this group, the mortality risk was comparable between HD and ol-HDF patients, both overall and in thirds of the convection volume. Subdivision of CVD mortality in fatal cardiac, non-cardiac and unclassified CVD showed that ol-HDF was only associated with a lower risk of cardiac casualties [0.64 (0.61; 0.90)]. Annual mortality rates also suggest that the reduction in CVD death is mainly due to a decrease in cardiac fatalities, including both ischaemic heart disease and congestion. Overall, 32 and 75 patients, respectively, need to be treated by high-volume HDF (HV-HDF) to prevent one all-cause and one CVD death, respectively, per year. Conclusion. The beneficial effect of ol-HDF on all-cause and CVD mortality appears to be mainly due to a reduction in fatal cardiac events, including ischaemic heart disease as well as congestion. In HV-HDF, the NNT to prevent one CVD death is 75 per year

Mortality reduction by post-dilution online-haemodiafiltration : A cause-specific analysis

Background. From an individual participant data (IPD) meta-analysis from four randomized controlled trials comparing haemodialysis (HD) with post-dilution online-haemodiafiltration (ol-HDF), previously it appeared that HDF decreases all-cause mortality by 14% (95% confidence interval 25; 1) and fatal cardiovascular disease (CVD) by 23% (39; 3). Significant differences were not found for fatal infections and sudden death. So far, it is unclear, however, whether the reduced mortality risk of HDF is only due to a decrease in CVD events and if so, which CVD in particular is prevented, if compared with HD. Methods. The IPD base was used for the present study. Hazard ratios and 95% confidence intervals for cause-specific mortality overall and in thirds of the convection volume were calculated using the Cox proportional hazard regression models. Annualized mortality and numbers needed to treat (NNT) were calculated as well. Results. Besides 554 patients dying from CVD, fatal infections and sudden death, 215 participants died from 'other causes', such as withdrawal from treatment and malignancies. In this group, the mortality risk was comparable between HD and ol-HDF patients, both overall and in thirds of the convection volume. Subdivision of CVD mortality in fatal cardiac, non-cardiac and unclassified CVD showed that ol-HDF was only associated with a lower risk of cardiac casualties [0.64 (0.61; 0.90)]. Annual mortality rates also suggest that the reduction in CVD death is mainly due to a decrease in cardiac fatalities, including both ischaemic heart disease and congestion. Overall, 32 and 75 patients, respectively, need to be treated by high-volume HDF (HV-HDF) to prevent one all-cause and one CVD death, respectively, per year. Conclusion. The beneficial effect of ol-HDF on all-cause and CVD mortality appears to be mainly due to a reduction in fatal cardiac events, including ischaemic heart disease as well as congestion. In HV-HDF, the NNT to prevent one CVD death is 75 per year