24 research outputs found

    Diseño de un programa de bioseguridad en el laboratorio de medicamentos del INSPI 2014 -2015

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    Pdf.El mundo de la investigación diagnóstica es un escenario de complejidad a los cuales no pueden dar respuesta métodos tradicionales. El éxito para adaptarse a la globalización en el mercado de la salud exige una serie de conocimientos y habilidades. Los profesionales de la salud frente al reto de las crecientes expectativas del público deben responder a sus expectativas. Por las mismas razones, es necesario desarrollar nuevos modelos de gestión por las mayores exigencias para que los laboratorios clínicos utilicen sus recursos de manera efectiva y se desempeñen con calidad y eficiencia. En los últimos tiempos, el concepto de “calidad” se ha hecho preponderante, originándose en el área de la industria la filosofía de la Calidad llegando posteriormente al campo de la salud. Su objetivo es sentar las bases que permitan la utilización de herramientas innovadoras en la gestión de los laboratorios clínicos que lleven a una optimización de capacidades y recursos. El estudio se focalizara en la revisión de los antecedentes que existen en la bibliografía acerca de la gestión de los laboratorios clínicos, las características básicas de la Gestión Clínica y sus posibilidades prácticas de aplicación en dichos laboratorios El propósito de la presente investigación es desarrollar un “diseño de un programa de bioseguridad en el laboratorio de medicamentos del INSPI que sirva para optimizar la experiencia acumulada y generar propuestas modernas que garanticen la bioseguridad tanto para los profesionales como para los usuarios de los servicios. Al término de la investigación y con los resultados de la fase de diagnóstico se presentará en formato de manual el programa de bioseguridad que será de utilidad científica y social PALABRAS CLAVES: métodos, bioseguridad, calidad programaThere search world diagnosed is a complexity which our traditional methods may not respond. The success to adapt to globalization in the market of health requires a series of knowledge and skills. The health professionals face the challenge of rising expectations of the public should respond to their expectations. For the same reasons, it’s necessary to develop new The success to adapt to globalization in the market of health requires a series of knowledge and skills. The health professionals face the challenge of rising expectations of the public should respond to their expectations. For the same reasons, it is necessary to develop new models of management by the highest standards of clinical laboratories utilize their resources effectively and perform with quality and efficiency. In recent times, the concept of "quality" has become dominant, originating in the area of the industry the quality philosophy and late reaching the field of health. The purpose of the present research is to develop a "design of a bio safety program. In the laboratory of medicines of the INSPI which serve to optimize the experience and create modern proposals that ensure biosecurity for both professionals and users of the services At the end of the investigation and the results of the diagnostic phase will be presented in manual format the program of bio-security which will be useful in scientific and social keywords: methods, keywords: methods, biosafety, quality, progra

    Cardiac magnetic resonance predictors of left ventricular remodelling following acute ST elevation myocardial infarction: The VavirimS study

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    Left ventricular (LV) remodelling (REM) ensuing after ST-elevation myocardial infarction (STEMI), has typically been studied by echocardiography, which has limitations, or cardiac magnetic resonance (CMR) in early phase that may overestimate infarct size (IS) due to tissue edema and stunning. This prospective, multicenter study investigated LV-REM performing CMR in the subacute phase, and 6 months after STEMI

    Influence of β-Cyclodextrin Methylation on Host-Guest Complex Stability: A Theoretical Study of Intra- and Intermolecular Interactions as Well as Host Dimer Formation

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    Understanding the non-covalent interactions in host-guest complexes is crucial to their stability, design and applications. Here, we use density functional theory to compare the ability of β-cyclodextrin (β-CD) and heptakis(2,6-di-O-methyl)-β-cyclodextrin (DM-β-CD) to encapsulate the model guest phenol. For both macrocycles, we quantify the intramolecular interactions before and after the formation of the complex, as well as the intermolecular host-guest and host-host dimer interactions. These are individually classified as van der Waals interactions or hydrogen bonds, respectively. The results show a stronger intramolecular binding energy of β-CD, with the absolute difference being −5.53 kcal/mol relative to DM-β-CD. Consequently, the intermolecular interactions of both cyclodextrins with phenol are affected, such that the free binding energy calculated for the DM-β-CD/phenol complex (−5.23 kcal/mol) is ≈50% more negative than for the complex with β-CD (−2.62 kcal/mol). The latter is in excellent agreement with the experimental data (−2.69 kcal/mol), which validates the level of theory (B97-3c) used. Taken together, the methylation of β-CD increases the stability of the host-guest complex with the here studied guest phenol through stronger van der Waals interactions and hydrogen bonds. We attribute this to the disruption of the hydrogen bond network in the primary face of β-CD upon methylation, which influences the flexibility of the host toward the guest as well as the strength of the intermolecular interactions. Our work provides fundamental insights into the impact of different non-covalent interactions on host-guest stability, and we suggest that this theoretical framework can be adapted to other host-guest complexes to evaluate and quantify their non-covalent interactions

    Preliminary study of the Salvia collection in Hanbury Botanical Gardens

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    Si riportano i risultati preliminari del lavoro di catalogazione effettuato presso i Giardini Botanici Hanbury (Ventimiglia, Italia) delle specie di Salvia appartenenti alla locale collezione. Il criterio seguito \ue8 stato quello fitogeografico, in base alle diverse aree di origine, seguendo la sistematica del genere riformata da Alziar ed Hedge. Le zone geografiche di biodiversit\ue0 del genere sono sei: Stati centro-meridionali degli U.S.A. e California, 40 taxa (17 in California); Messico, 300 taxa; Sud-America 210 taxa; Europa, Africa Mediterranea ed Asia Occidentale, complessivamente 210 taxa; Sud-Africa, 70 taxa; Asia Orientale, 90 taxa. L\u2019elenco dei taxa del genere Salvia L. riportato nel catalogo Hortus mortolensis, pubblicato nel 1995 da Campodonico, \ue8 stato confrontato sia con i taxa presenti attualmente, sia con quelli presenti nei cataloghi precedenti, allo scopo di verificare se nel corso di oltre un secolo, l\u2019acclimatazione di specie provenienti da altre zone del mondo in questo Orto Botanico \ue8 stata efficace, e se quindi il clima ne consente una coltivazione ottimale. Sono state anche citate sinonimie evidenziate nel corso del tempo. References .Alziar, Gabriel: \u201cCatalogue synonimique des Salvia L. du monde (Lamiaceae)" Biocosme, Revue d\u2019histoire naturelle, 5 (3-4)-1988 ; 6 (4)-1989 ; 9 (2-3)-1992 ; 10 (3-4)-1993. Hedge, I.C. 1974: \u201cA revision of Salvia in Africa including Madagascar and the Canary Island\u201d. Campodonico, Pier Giorgio; Orsino, Francesco; Cerkvenik, Cristina: 1995 \u201cEnumeratio plantarum in Horto Mortolensi cultarum\u201d Microart\u2019s Eds., Recco (Genova, Italy). Erhardt, Walter; G\uf6tz, Erich; B\uf6deker, Nils; Seybold, Siegmund 2000: \u201cZander \u2013 Dictionary of plant names\u2019\u2019 16th edition. Eugen Ulmer GmbH & Co. Eds., Stuttgart, Germany. Cronemeyer, Gustav 1889: \u201cSystematic catalogue of plants growing in the open air in the garden of Thomas Hanbury F.L.S.\u201d Printed by G. A. Koenig, Erfurt. Dinter, Kurt 1897: \u201cAlphabetical Catalogue of Plants growing in the open air in the garden of Thomas Hanbury F.L.S.\u201d Printed by Waser Brothers, Genoa. Berger, Alwin 1912: \u201cHortus mortolensis\u201d. West, Newman & Co., Hatton Garden, London. Ercoli, Mario; Lorenzi, Maurizio and Lady Hanbury O.B.E. 1938: \u201cLa Mortola Garden Hortus Mortolensis.\u201d. Oxford University Press. London: Humphrey Milford

    Educación psicomotriz en el desarrollo de la expresión corporal de los niños de 4 años de la I.E.I. N° 086 “Divino Niño Jesús”- Huacho, durante el año escolar 2020

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    El niño utiliza su cuerpo como medio de comunicación y expresión desde el nacimiento. A medida que crece e incorpora nuevos movimientos a sus acciones, con el fin de establecer una mayor y mejor relación con el entorno natural y social, su cerebro responderá a determinadas actividades físicas y ejerce sus habilidades en consecuencia. Los reflejos primitivos de los bebés se integran en patrones de movimiento cada vez más complejos y especializados durante su desarrollo. El desarrollo de los niños, especialmente la educación inicial, es la máxima prioridad de los maestros, por lo que se debe prestar especial atención a la educación psicomotriz, para que la capacidad de expresión corporal de los niños se desarrolle por completo, de modo que experimenten la capacidad de la imaginación y la creatividad. El objetivo de este estudio es, determinar la influencia que ejerce la educación psicomotriz en el desarrollo de la expresión corporal de los niños de 4 años de la I.E.I. No 086 “Divino niño Jesús”-Huacho, durante el año escolar 2020. Para este fin la pregunta de investigación es la siguiente: ¿De qué manera influye la educación psicomotriz en el desarrollo de la expresión corporal de los niños de 4 años de la I.E.I. No 086 “Divino niño Jesús”-Huacho, durante el año escolar 2020? Al estudiar la lista de verificación de la educación psicomotriz en el desarrollo de la expresión corporal de los niños de 4 años, la misma que fue aplicada por el equipo de apoyo del investigador también optó el mismo método. En este caso, la lista de verificación consta de 15 ítems en una tabla de doble entrada y se evalúan 3 alternativas. A razón de contar con una población bastante pequeña, se decidió aplicar el instrumento de recolección de datos a la población en su conjunto. Se analizan las siguientes dimensiones; esquema corporal, estructuración espacial, coordinación y equilibrio para la educación psicomotriz; y las dimensiones; cuerpo, espacio y tiempo de la variable desarrollo de la expresión corporal Teniendo esto en cuenta, se aconseja a los maestros que dejen de aprender y, a menudo, busquen un método de enseñanza mejor y más eficaz para llegar a los niños, de modo que los estudiantes obtengan los mejores resultados de aprendizajeTesi

    Effect of the nature of the nucleophile and solvent on an SNAr reaction

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    The reaction of 2,4-dinitrobenzenesulfonyl chloride toward propylamine was kinetically evaluated in 19 organic solvents and 10 ionic liquids as reaction media. This study was compared with a previous study to experimentally show that solvent effects and the nature of the reacting pair drastically affect the reaction rate and the reaction mechanism. While the reaction of the reference electrophile 2,4-dinitrobenzenesulfonyl chloride with piperidine is favored in polar solvents with the ability to donate or accept hydrogen bonds, the reaction with propylamine is favored in solvents with the ability to accept hydrogen bonds.Fondecyt 11140172 1150759 project ICM-MINECON - Fondo de Innovacion para La Competitividad Del Ministerio de Economia, Fomento y Turismo, Chile RC-130006 - CILIS Instituto de Ciencias e Innovacion en Medicina (ICIM-CAS UDD) 312006

    A Natural Deep Eutectic Solvent Formulated to Stabilize β-Lactam Antibiotics

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    Abstract β-lactam antibiotics, such as penicillin share a very unstable chemical structure. In water-based solutions, such as those used for clinical applications, the β-lactam ring is readily opened due to a nucleophilic or electrophilic attack, leading to the loss of antimicrobial activity. Since the achievement and maintenance of optimum therapeutic levels of β-lactam antibiotics is critical for the resolution of many infectious clinical situations, and to avoid antibiotic resistance generation, the design of new non-aqueous dosage forms is urgent. Recently, natural deep eutectic solvents (NADES) have emerged as alternative non-toxic and non-aqueous solvents for different biomedical applications. In this work, we formulated and characterized a NADES composed by betaine and urea (BU). Using this solvent, we evaluated the stability of clavulanic acid (CLV) and imipenem (IMP) and characterized their antimicrobial activities calculating the minimal inhibitory concentration. Characterization of BU solvent by infrared spectroscopy (IR) and nuclear magnetic resonance spectroscopy (NMR) indicated that the obtained solvent has a microstructure mainly based on hydrogen bonding interactions and water addition strongly affects its dynamic. The stability of β-lactam antibiotic IMP and CLV using this solvent was increased by 7 fold and 2.5 fold respectively compared to water when analysed seven days after being dissolved. Microbiological assays showed that antibacterial activity at day seven was significantly decreased for both CLV and IMP when dissolved in water, while no change in their antibacterial properties was observed when antibiotics were dissolved in BU. The increased stability of IMP and CLV in BU may be related to the inert behaviour of the solvent and the higher dynamic restriction that helps antibiotics to maintain a more stable conformation. These data suggest the potential use of BU as a solvent to prevent degradation of β-lactam antibiotics

    [Evolution of the diagnostic accuracy of CT in clinical staging of patients with Hodgkin's disease].

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    From 1983 through 1989, 141 untreated patients with Hodgkin's disease underwent CT of the abdomen. They subsequently underwent staging laparotomy plus splenectomy and multiple biopsies of liver and lymph nodes, at the Institute of Radiology and Hematology, University "La Sapienza", Rome. CT results were compared with surgical findings to evaluate CT sensitivity, specificity, and overall accuracy. The cases from this series were divided into two groups depending on the characteristics of the CT scanners employed. From 1983 to 1985, 78 patients were examined with 2nd-generation CT units; from 1986 to 1989, 63 patients underwent CT performed with 3rd-generation scanners. The results from the two groups were analyzed according to these parameters. A total number of 622 biopsies were performed, of spleen, liver, and lymph nodes. CT sensitivity, specificity, and overall accuracy were: 22.9% (group I) vs 43.7% (group II), 83.1% vs 92%, and 68.4% vs 81.2% for lymph nodes; 28.1% vs 36.3%, 93.5% vs 98%, and 66.7% vs 87.3% for the spleen, and 12.5% vs 42.8%, 97.1% vs 98.2%, and 88.5% vs 92.1% for the liver. Our results demonstrate an obvious increase in reliability with newer units, even though a high percentage of false-negatives were still observed in our series, which caused understaging in 19.4% of cases vs 24.4% in group I
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