Lofting of low speed ejecta produced in the DART experiment and production of a dust cloud

NASA sent the DART (Double Asteroid Redirection Test) mission to impact Dimorphos, the satellite of the asteroid binary system (65803) Didymos. DART will release LICIACube prior to impact to obtain high-resolution post-impact images. The impact will produce a crater and a large amount of material ejected at high speed (several tens of m/s), producing an ejecta cone that will quickly disperse. We analyzed an additional effect: the lofting of material at low velocity due to the generation of seismic waves that propagate inside Dimorphos, producing surface shaking far from the impact point. We divide the process into different stages: from the generation of impact-induced waves, the interaction of them with surface particles, the ejection of dust particles at velocities, and the prediction of the observability of the dust coma and trail. We anticipate the following observable effects: i) generation of a dust cloud that will produce a hazy appearance of Dimorphos' surface, detectable by LICIACube; ii) brightness increase of the binary system due to enhancement of the cross section produced by the dust cloud; iii) generation of a dust trail, similar to those observed in some Active Asteroids, which can last for several weeks after impact. Numerical prediction of the detectability of these effects depends on the amount and size distribution of ejected particles, which are largely unknown. In case these effects are observable, an inversion method can be applied to compute the amount of ejected material and its velocity distribution, and discuss the relevance of the shaking process.Comment: 12 pages, 13 figure

Ground-based observability of Dimorphos DART impact ejecta: Photometric predictions

The Double Asteroid Redirection Test (DART) is a NASA mission intended to crash a projectile on Dimorphos, the secondary component of the binary (65803) Didymos system, to study its orbit deflection. As a consequence of the impact, a dust cloud will be be ejected from the body, potentially forming a transient coma- or comet-like tail on the hours or days following the impact, which might be observed using ground-based instrumentation. Based on the mass and speed of the impactor, and using known scaling laws, the total mass ejected can be roughly estimated. Then, with the aim to provide approximate expected brightness levels of the coma and tail extent and morphology, we have propagated the orbits of the particles ejected by integrating their equation of motion, and have used a Monte Carlo approach to study the evolution of the coma and tail brightness. For typical power-law particle size distribution of index --3.5, with radii r$_{rmin}$=1 $\mu$m and r$_{max}$=1 cm, and ejection speeds near 10 times the escape velocity of Dimorphos, we predict an increase of brightness of $\sim$3 magnitudes right after the impact, and a decay to pre-impact levels some 10 days after. That would be the case if the prevailing ejection mechanism comes from the impact-induced seismic wave. However, if most of the ejecta is released at speeds of the order of $\gtrsim$100 $\mathrm{m\; s^{-1}}$, the observability of the event would reduce to a very short time span, of the order of one day or shorter.Comment: Accepted by MNRAS, June 30, 202

Ground-based obser v ability of Dimor phos DART impact ejecta: photometric predictions

The Double Asteroid Redirection Test (DART) is a NASA mission intended to crash a projectile on Dimorphos, the secondary component of the binary (65803) Didymos system, to study its orbit deflection. As a consequence of the impact, a dust cloud will be be ejected from the body, potentially forming a transient coma- or comet-like tail on the hours or days following the impact, which might be observed using ground-based instrumentation. Based on the mass and speed of the impactor, and using known scaling laws, the total mass ejected can be roughly estimated. Then, with the aim to provide approximate expected brightness levels of the coma and tail extent and morphology, we have propagated the orbits of the particles ejected by integrating their equation of motion, and have used a Monte Carlo approach to study the evolution of the coma and tail brightness. For typical power-law particle size distribution of index –3.5, with radii r rmin = 1 μm and r max = 1 cm, and ejection speeds near 10 times the escape velocity of Dimorphos, we predict an increase of brightness of ∼3 magnitudes right after the impact, and a decay to pre-impact levels some 10 d after. That would be the case if the pre v ailing ejection mechanism comes from the impact-induced seismic wave. Ho we ver, if most of the ejecta is released at speeds of the order of 100 m s −1 , the observability of the event would reduce to a very short time span, of the order of 1 d or shorter.ANII: FCE_1_2019_1_15645

Transcriptomic Identification of Iron-Regulated and Iron-Independent Gene Copies within the Heavily Duplicated Trichomonas vaginalis Genome

Gene duplication is an important evolutionary mechanism and no eukaryote has more duplicated gene families than the parasitic protist Trichomonas vaginalis. Iron is an essential nutrient for Trichomonas and plays a pivotal role in the establishment of infection, proliferation, and virulence. To gain insight into the role of iron in T. vaginalis gene expression and genome evolution, we screened iron-regulated genes using an oligonucleotide microarray for T. vaginalis and by comparative EST (expressed sequence tag) sequencing of cDNA libraries derived from trichomonads cultivated under iron-rich (+Fe) and iron-restricted (−Fe) conditions. Among 19,000 ESTs from both libraries, we identified 336 iron-regulated genes, of which 165 were upregulated under +Fe conditions and 171 under −Fe conditions. The microarray analysis revealed that 195 of 4,950 unique genes were differentially expressed. Of these, 117 genes were upregulated under +Fe conditions and 78 were upregulated under −Fe conditions. The results of both methods were congruent concerning the regulatory trends and the representation of gene categories. Under +Fe conditions, the expression of proteins involved in carbohydrate metabolism, particularly in the energy metabolism of hydrogenosomes, and in methionine catabolism was increased. The iron–sulfur cluster assembly machinery and certain cysteine proteases are of particular importance among the proteins upregulated under −Fe conditions. A unique feature of the T. vaginalis genome is the retention during evolution of multiple paralogous copies for a majority of all genes. Although the origins and reasons for this gene expansion remain unclear, the retention of multiple gene copies could provide an opportunity to evolve differential expression during growth in variable environmental conditions. For genes whose expression was affected by iron, we found that iron influenced the expression of only some of the paralogous copies, whereas the expression of the other paralogs was iron independent. This finding indicates a very stringent regulation of the differentially expressed paralogous genes in response to changes in the availability of exogenous nutrients and provides insight into the evolutionary rationale underlying massive paralog retention in the Trichomonas genome

Dimorphos's Orbit Period Change and Attitude Perturbation due to Its Reshaping after the DART Impact

On 2022 September 26 (UTC), NASA's Double Asteroid Redirection Test (DART) mission achieved a successful impact on Dimorphos, the secondary component of the near-Earth binary asteroid system (65803) Didymos. Subsequent ground-based observations suggest a significant reshaping of Dimorphos, with its equatorial axis ratio changing from 1.06 to ∼1.3. Here we report the effects of this reshaping event on Dimorphos's orbit and attitude. Given the reported reshaping magnitude, our mutual dynamics simulations show that approximately 125 s of the observed 33 minute orbit period change after the DART impact may have resulted from reshaping. This value, however, is sensitive to the precise values of Dimorphos's post-impact axis ratios and may vary by up to 2 times that amount, reaching approximately 250 s within the current uncertainty range. While the rotational state of the body is stable at the currently estimated axis ratios, even minor changes in these ratios or the introduction of shape asymmetry can render its attitude unstable. The perturbation to Dimorphos's orbital and rotational state delivered by the impact directly, combined with any reshaping, leads to a strong possibility for a tumbling rotation state. To accurately determine the momentum enhancement factor (β) through measurements by the European Space Agency's Hera spacecraft and to evaluate the effectiveness of the kinetic deflection technique for future planetary defense initiatives, the effects of reshaping should not be overlooked.This work was supported in part by the DART mission, NASA contract 80MSFC20D0004 to JHU/APL. R.N. acknowledges support from NASA/FINESST (NNH20ZDA001N). S.D.R. and M.J. acknowledge support from the Swiss National Science Foundation (project number 200021_207359). P.M. acknowledges funding support from the French Space Agency CNES and The University of Tokyo. P.P. acknowledges support from the grant Agency of the Czech Republic, grant 23-04946S. S.R.S. acknowledges support from the DART Participating Scientist Program, grant No. 80NSSC22K0318. A.C.B. and P.Y.L. acknowledge funding by the NEO-MAPP project 717 GA 870377, EC H2020-SPACE-718 2018-2020/H2020-SPACE-2019, and by MICINN (Spain) PGC2021, PID2021-125883NB-C21. P.Y.L. acknowledges funding from the European Space Agency OSIP contract N.4000136043/21/NL/GLC/my. A portion of this work was carried out at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration (No. 80NM0018D0004)

Identification of a Simple Sequence Repeat molecular-marker set for large-scale analyses of pear germplasm

Simple Sequence Repeats (SSR) are molecular markers suitable to assess the genetic variation of germplasm resources; however, large-scale SSR use requires protocol optimization. The present work aimed to identify SSR markers, developed for pear and other fruit species that are effective in characterizing pear germplasm collections and in demonstrating their use in providing support for genetic breeding programs. From a total of 62 SSR markers investigated, 23 yielding reproducible and polymorphic patterns were used to genotype a sample of 42 pear accessions of the Brazilian Pear Germplasm Bank (PGB). When compared to these 23 SSR markers, a subset of eleven markers, selected based on He, PIC and PId, was used to distinguish individual accessions and perform cluster analysis with similar efficacy. Genetic diversity analysis clustered the European, Japanese and Chinese accessions in distinct groups. This markers subset constitutes a valuable tool for several applications related to pear genetic resources management and breeding

Antigenic Proteins Involved in Occupational Rhinitis 1 and Asthma Caused by Obeche Wood (Triplochiton Scleroxylon)

Background Obeche wood dust is a known cause of occupational asthma where an IgE-mediated mechanism has been demonstrated. Objective To characterize the allergenic profile of obeche wood dust and evaluate the reactivity of the proteins by in vitro, ex vivo and in vivo assays in carpenters with confirmed rhinitis and/or asthma Materials and methods An in-house obeche extract was obtained, and two IgE binding bands were purified (24 and 12 kDa) and sequenced by N-terminal identity. Specific IgE and IgG, basophil activation tests and skin prick tests (SPTs) were performed with whole extract and purified proteins. CCD binding was analyzed by ELISA inhibition studies. Results Sixty-two subjects participated: 12 with confirmed occupational asthma/rhinitis (ORA+), 40 asymptomatic exposed (ORA−), and 10 controls. Of the confirmed subjects, 83% had a positive SPT to obeche. There was a 100% recognition by ELISA in symptomatic subjects vs. 30% and 10% in asymptomatic exposed subjects and controls respectively (p<0.05). Two new proteins were purified, a 24 kDa protein identified as a putative thaumatin-like protein and a 12 kDa gamma-expansin. Both showed allergenic activity in vitro, with the putative thaumatin being the most active, with 92% recognition by ELISA and 100% by basophil activation test in ORA+ subjects. Cross-reactivity due to CCD was ruled out in 82% of cases. Conclusions Two proteins of obeche wood were identified and were recognized by a high percentage of symptomatic subjects and by a small proportion of asymptomatic exposed subjects. Further studies are required to evaluate cross reactivity with other plant allergens

A model for co-expression pattern analysis of genes implicated in angiogenesis and tumour cell invasion in cervical cancer

To date, numerous genes have been identified which are involved in both tumour neovascularisation (angiogenesis) and tumour cell invasion, and most of them are also expressed to some extent under normal physiological conditions. However, little is known about how these genes co-express in these settings. This study was undertaken to quantitate mRNA levels in normal and malignant cervical tissues of nine selected genes (VEGF121, VEGF165, VEGF189, VEGF-C, eIF-4E, b-FGF, TSP-2, MMP-2 and MMP-9) implicated in the above processes using real-time quantitative RT–PCR. In addition, the Spearman's rank correlation was used to determine their co-expression patterns. The transcript levels for the different VEGF-A splice variants (VEGF121, VEGF165, VEGF189) were at least 10-fold higher in the cancer cases, with the highest levels in the primary tumours demonstrating lympho-vascular space involvement. The lymphangiogenic factor VEGF-C and MMP-9 were upregulated 130- and 80-fold respectively in cervical cancers. The highest levels of VEGF-C mRNA were found in the lymph-node positive group. The transcript levels for b-FGF were similar in normal cervical tissue and early-stage cervical cancer, however, higher levels were found in the cervical cancers with advanced stage disease. Comparing gene transcript levels between recurrent and non-recurrent cervical cancer patients revealed significant differences (P=0.038) in transcript levels for the angiogenesis inhibitor TSP-2, with the highest levels in non-recurrent cases. Co-expression pattern analysis in normal cervical tissue revealed highly significant co-expressions (P<0.0001) between TSP-2 and most other genes analysed (VEGF121, VEGF165, VEGF-C, b-FGF and MMP-2). In cervical cancer, TSP-2 appears only to be highly co-expressed with MMP-2 (P<0.0001). In contrast to normal cervical tissue, we found a highly significant co-expression (P<0.0001) between MMP-9 and VEGF189 in cervical cancer. The combined application of real-time quantitative RT–PCR and Spearman's rank correlation identifies gene transcripts which are simultaneously co-expressed. Our results revealed a significant co-expression between the angiogenesis inhibitor TSP-2 and most other genes analysed in normal cervical tissue. In cervical cancer, we found a strong upregulation of VEGF-C and MMP-9 mRNA, with a highly significant co-expression between MMP-9 and VEGF189