Multiplex PCR assay for the simultaneous detection of bacterial pathogens in rainbow trout

Accurate, rapid, and specific methods are required to identify pathogens for controlling bacterial diseases in fish. Various microbiological and biochemical methods are used to isolate and identify pathogens, but they are not adequately efficient and/or accurate. For example, distinguishing between Streptococcus iniae and Lactococcus garvieae is difficult via microbiological tests. Moreover, these bacteria often cause concurrent infections. Therefore, early and accurate identification of bacterial pathogens in trout is of utmost importance to prevent possible damage. Because of this, the multiplex polymerase chain reaction (multiplex PCR) method was optimized for simultaneous identification of bacterial infections caused by Yersinia ruckeri, S. iniae, and L. garvieae, three harmful bacteria of great importance in aquaculture. Multiplex PCR reaction was optimized on control samples, and the sensitivity and specificity of the test were evaluated. Multiplex PCR reaction was then performed on DNA isolated from samples collected from fish-breeding farms and transported to the laboratory. Multiplex PCR was found to be an accurate and sensitive method to identify simultaneously different species of bacteria

Étude des matériaux de construction en terre crue des sites antiques de Rirha (Maroc)

Les sites successifs de Rirha (Sidi Slimane, Maroc) sont installés dans la riche plaine agricole du Gharb. La zone archéologique, en domaine alluvial, est actuellement enserrée dans un méandre récent du Beht. La fouille porte sur un tell dont l’occupation débute à l’âge du Fer, et qui est vraisemblablement agrandi durant l’époque romaine, puis réoccupé à l’époque médiévale entre le xiie et le xive siècle. Pour la période maurétanienne, la terre crue – plus particulièrement la brique – est exc..

Comparison of long term tropospheric ozone trends measured by lidar and ECC ozonesondes from 1991 to 2010 in Southern France

International audienceECC (Electrochemical Concentration Cell) ozonesondes and UV DIAL (DIfferential Absorption Lidar) measurements have been carried out simultaneously at OHP (Observatoire de Haute Provence, 44°N, 6.7°E, 690 m) since 1991. A unique long-term trend assessment by two different instruments operated routinely at the same location is possible. Air mass trajectories have been calculated for all the ozone observations available at OHP. The bias between the seasonal mean calculated with lidar and ECC ozone vertical profiles for 4 time- periods of 5 years is 0.6 ppbv in the free troposphere (4-8 km). Larger differences (> 10 ppbv) are explained by the need for clear sky conditions during lidar observations. The measurements of both instruments have been combined to decrease the impact of short-term atmospheric variability on the trend estimate

Optimal Rate Adaptation with Integer Linear Programming for the Scalable Extension of H264/AVC

5noAdaptation for scalable video is one of the recent challenges in video distribution over modern networks, which are heterogeneous both in terms of available bandwidth and user end terminal capability. Scalable Video Coding offers the possibility to adapt the content following the “quality layer” abstraction. In this work we present a new method to optimally define quality layers using Integer Linear Programming and distortion models. The performances of the proposed approach are comparable with the state-of-the-art methods, but they are obtained with strong complexity reduction and augmented flexibility.partially_openpartially_openLima L.; Mauro M.; Anselmo T.; Alfonso D.; Leonardi R.Lima, Livio; Mauro, Massimo; Anselmo, T.; Alfonso, D.; Leonardi, Riccard

A Jackson Portrait for Jamestown, A Magnet in the Room

As Robert H. Jackson gained prominence in law practice and national government, he had particularly close ties to the city of Buffalo and to its University and School of Law. Jackson briefly practiced law in Buffalo for a year near the start of his career. He continued thereafter to handle Buffalo cases and represent Buffalo clients even though his practice was based in Jamestown. In 1946, Jackson received an honorary degree from the University of Buffalo at its centennial commemoration and spoke then about his just-completed Nuremberg experiences, including the evidence on German persecution of minorities. In 1951, Justice Jackson\u27s speech on Wartime Security and Liberty under Law was the first James McCormack Mitchell lecture at the Buffalo School of Law. Many of the great legal figures in Buffalo and the University of Buffalo history, including Wild Bill Donovan, Louis Jaffe, John Lord O\u27Brian, Charles Sears and Frank Shea, also were among Jackson\u27s close mentors, colleagues, assistants and friends. Although Jackson is remembered prominently in American and Supreme Court history and in international law, he is becoming increasingly visible today due to notable efforts based in his home region and adult hometown. In early 2001, the Robert H. Jackson Center was established in Jamestown and on the Internet to advance Jackson\u27s legacy through educational programming, exhibits and special events that connect his life and ideas to today\u27s issues and challenges. The Buffalo Law Review contributes to that effort, and it reinforces the strong ties that bind Jackson to its academic home, by publishing here the portrait of Jackson that New York painter Lurabel Long Colburn donated to the Robert H. Jackson Center and remarks from the occasion of the portrait\u27s unveiling in July 2002

A Jackson Portrait for Jamestown, A Magnet in the Room

As Robert H. Jackson gained prominence in law practice and national government, he had particularly close ties to the city of Buffalo and to its University and School of Law. Jackson briefly practiced law in Buffalo for a year near the start of his career. He continued thereafter to handle Buffalo cases and represent Buffalo clients even though his practice was based in Jamestown. In 1946, Jackson received an honorary degree from the University of Buffalo at its centennial commemoration and spoke then about his just-completed Nuremberg experiences, including the evidence on German persecution of minorities. In 1951, Justice Jackson\u27s speech on Wartime Security and Liberty under Law was the first James McCormack Mitchell lecture at the Buffalo School of Law. Many of the great legal figures in Buffalo and the University of Buffalo history, including Wild Bill Donovan, Louis Jaffe, John Lord O\u27Brian, Charles Sears and Frank Shea, also were among Jackson\u27s close mentors, colleagues, assistants and friends. Although Jackson is remembered prominently in American and Supreme Court history and in international law, he is becoming increasingly visible today due to notable efforts based in his home region and adult hometown. In early 2001, the Robert H. Jackson Center was established in Jamestown and on the Internet to advance Jackson\u27s legacy through educational programming, exhibits and special events that connect his life and ideas to today\u27s issues and challenges. The Buffalo Law Review contributes to that effort, and it reinforces the strong ties that bind Jackson to its academic home, by publishing here the portrait of Jackson that New York painter Lurabel Long Colburn donated to the Robert H. Jackson Center and remarks from the occasion of the portrait\u27s unveiling in July 2002

Genotypic and computational sequence analysis of ALADIN gene causing Allgrove syndrome

Background: Allgrove syndrome is autosomal recessive disorder, the gene involved in this syndrome is known as ALADIN located close to type 2 keratin gene cluster on chromosome 12q13 whose function is to control the nucleocytoplasmic trafficking and also affects the nuclear pore complexes. Mutation in this gene cause triple A syndrome. The aim of research was to analyze the mutational changes in ALADIN gene, formation of 3D Structure of normal and mutated protein and differentiation of normal and mutated protein.Methods: Genotyping by using tetra arm PCR and Sequence analyses of coding region of ALADIN gene was done in two families having affected children with Allgrove’s syndrome.Results: Point mutation in exon 1 and alteration in 3D structure of protein was observed by using VMD (Visual molecular dynamics) that shows truncation, absence of few amino acid and structural modification of proteins which alters in transportation ability.Conclusion: It is concluded from the study that proper structure and function of NPC (nuclear pore complex) binding protein is necessary in normal body function and if any mutation is present in ALADIN gene it can cause symptoms of rare Allgrove’s syndrome.Keywords: ALADIN gene; 3D protein structure; Triple A syndrom

Análisis objetivo y subjetivo del ruido emitido por máquinas

El ruido industrial producido por las máquinas es una fuente de ruido muy extendida en los países industrializados, y que afecta tanto a trabajadores como a vecinos. Dada la gran importancia de esta fuente de ruido, el grupo de Investigación y Desarrollo en Acústica (IDEA) de la Escuela Politécnica de Cuenca ha llevado a cabo diversas investigaciones centradas en la medida y evaluación del ruido producido por las mismas. Dichas investigaciones se han centrado en la evaluación tanto objetiva como subjetiva del ruido producido las máquinas. Desde el punto de vista objetivo, se ha evaluado la potencia acústica emitida por máquinas de diversos tamaños, desde máquinas pequeñas (taladros, amoladoras, etc.) a máquinas grandes (excavadoras, dumpers, etc.), analizando el método estandarizado que mejor se adecúa a la medida de potencia acústica en cada uno de estos grupos y la emisión acústica de dichas máquinas. Desde el punto de vista psicoacústico, se han evaluado los parámetros psicoacústicos objetivos, permitiéndonos conocer en mayor detalle la emisión que presenta este tipo de máquinas. Además, se han pasado encuestas a distintas personas, con el fin de conocer la percepción subjetiva que tienen del ruido emitido por las mismas. Los resultados obtenidos con los distintos parámetros analizados (acústicos y psicoacústicos) permitió la elaboración de patrones acústicos y de un nuevo código de etiquetado de máquinas en el que se ponen de manifiesto, de forma clara y sencilla, las características del ruido emitido por las mismas y su percepción psicoacústica. Además, se han explorado nuevos procedimientos de medida en base a distintas técnicas de procesado, como por ejemplo la separación ciega de fuentes, que establecen un punto de partida que podrá permitir la medida de la potencia acústica de las máquinas evitando los problemas que presentan las técnicas estandarizadas actuales

Southeast Ohio Winter/Spring 2022

Table of contents: Set in StoneThe father of statehood’s old home still stands ... 4 Pleased to Meat You Fat Boy Q founder serves authentic BBQ ... 8 Safe Space and Coffee Cake Cafe owner understands the importance of inclusion ... 10 Family Tested, Tastebud Approved Two restaurants share a love for their customers .. 12 A Tale of Two Switzerlands Two towns claim to be the “The Switzerland of Ohio” ... 14 Patchwork Quilt barn trails started as an Adams County idea and grew into a national trend ... 16 Blood, Sweat and Alpaca Spit The Cammas know all their alpacas by name ... 19 Museum Attracts Tourists, Dull Pencils Paul Johnson’s legacy is now a museum in Logan ... 21 Connecting Cultures The Athens Asian American Alliance brings visibility and history ... 24 Off the Bench Lane Smith performs despite one blind eye ... 28 Nude Is Everyone’s Color Strip away self-doubt and societal expectations at Cedar Trails ... 30 Pioneer Timber Framing: Love for Craftsmanship Timber framing business builds handcrafted homes ... 34 Southeast Ohio Talks Trash Ramping recycling and conservation efforts ... 36 An Acre of ACRE Elementary school gets fresh start as an asset to Amesville ... 38 Connecting the Dots The Little Cities of Black Diamonds celebrates the coal mining region ... 40 Staying on Track Retired railroader and friends convert station to museum ... 42 John “John Boy” Pelletier An interview with the recently retired Jackson County broadcasting legend following a 53-year career ... 46https://ohioopen.library.ohio.edu/southeast-ohio/1020/thumbnail.jp