97 research outputs found
Comparative evaiuation of toxoplasmosis indirect fluorescent and SABINFELDMAN dye tests in a thousand human sera. A few unexpected results
Fluorescent antibody test for the serodiagnosis of American tPypanosomiasis. Technical modification employing preserved culture forms of Trypanosoma cruzi in a slide test
Comparative study between immunofluorescence and coproculture in the diagnosis of intestinal infections by enteropathogenic Escherichia coli
Analytical polyacrylamide-gel electrophoresis of aqueous Trypanosoma cruzi extracts. I – Chemical and immunological investigaticn of Y and Brasil strains antigenic components
Ocorrência de seis casos suspeitos, autóctones sorologicamente positivos ()
The authors present the first six possible autoctonus serologically positive cases of Chagas disease in farmers from municipality of Barcelos, in the State of Amazonas, Brazil. This region has many "piaçaba" palm trees (Leopoldinia piassaba) which harbor numerous triatomes in nature
Investigação epidemiológica de um caso de leishmaniose visceral autóctone da Grande São Paulo, Brasil
Foi descrita uma investigação epidemiológica realizada na zona urbana de Diadema, município da Grande São Paulo (Brasil), com a finalidade de elucidar a fonte de infecção e o mecanismo de transmissão de um caso de leishmaniose visceral autóctone da área. Foram realizados inquéritos sorológicos através da reação de imunofluorescência indireta na população humana (591 soros) e na população canina (55 soros), e levada a efeito pesquisa entomológica no local da residência do doente e em uma área de reserva florestal situada a 500m desta residência.Description of an epidemiological survey carried out in the urban area of Diadema, a district of Great S. Paulo (Brazil), with the aim of elucidating the source of infection and the mechanism of transmission of one case of visceral leishmaniasis in the area. Serological surveys were carried out (imunofluorescent test) in the human population (591 serums) and in the canine population (55 serums). Also an entomological survey was done in the neighbourhood of the patient's house and in a florest reserve 500m from the house
Synthetic Peptides Mimic gp75 from Paracoccidioides brasiliensis in the Diagnosis of Paracoccidioidomycosis
Paracoccidioidomycosis (PCM) is a systemic granulomatous disease, endemic in Latin America, caused by the thermal dimorphic fungus Paracoccidioides brasiliensis. Although some fungal antigens have already been characterized and used for serological diagnosis, cross-reactions have been frequently observed. Thus, the examination of fungal forms in clinical specimens or isolation of P. brasiliensis by culture is still the most frequent method for the diagnosis of this mycosis. In this study, a random peptide phage display library was used to select mimotopes of P. brasiliensis, which were employed as antigens in an indirect enzyme-linked immunosorbent assay. The protective monoclonal antibody against experimental PCM (anti-gp75) was used as molecular target to screen a phage display library. That approach led to a synthetic peptide named P2, which was synthesized and tested against PCM patients’ sera to check whether it was recognized. There was significant recognition of P2 by sera of untreated PCM patients when compared with normal human sera. Sera from treated PCM group, patients with other mycosis or co-infected with HIV had much lower recognition of P2 than untreated patient group. The test showed a sensitivity of 100 and 94.59% of specificity in relation to human sera control. These data indicate a potential use of P2 as diagnostic tool in PCM. Its application for serological diagnosis of PCM may contribute to the development and standardization of simpler, faster and highly reproducible immunodiagnostic tests at low cost
Chagas' disease: IgA, IgM and IgG antibodies to T. cruzi amastigote, trypomastigote and epimastigote antigens in acute and in different chronic forms of the disease
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