Plate boundary trench retreat and dextral shear drive intracontinental fault-slip histories: Neogene dextral faulting across the Gabbs Valley and Gillis Ranges, Central Walker Lane, Nevada

The spatial-temporal evolution of intracontinental faults and the forces that drive their style, orientation, and timing are central to understanding tectonic processes. Intracontinental NW-striking dextral faults in the Gabbs Valley–Gillis Ranges (hereafter referred to as the GVGR), Nevada, define a structural domain known as the eastern Central Walker Lane located east of the western margin of the North American plate. To consider how changes in boundary type along the western margin of the North American plate influenced both the initiation and continued dextral fault slip to the present day in the GVGR, we combine our new detailed geologic mapping, structural studies, and 40Ar/39Ar geochronology with published geologic maps to calculate early to middle Miocene dextral fault-slip rates. In the GVGR, Mesozoic basement is nonconformably overlain by a late Oligocene to Miocene sequence dominated by tuffs, lavas, and sedimentary rocks. These rocks are cut and offset by four primary NW-striking dextral faults, from east to west the Petrified Spring, Benton Spring, Gumdrop Hills, and Agai Pah Hills–Indian Head faults. A range of geologic markers, including tuff- and lava-filled paleovalleys, the southern extent of lava flows, and a normal fault, show average dextral offset magnitudes of 9.6 ± 1.1 km, 7.0 ± 1.7 km, 9.7 ± 1.0 km, and 4.9 ± 1.1 km across the four faults, respectively. Cumulative dextral offset across the GVGR is 31.2 ± 2.3 km. Initiation of slip along the Petrified Spring fault is tightly bracketed between 15.99 ± 0.05 Ma and 15.71 ± 0.03 Ma, whereas slip along the other faults initiated after 24.30 ± 0.05 Ma to 20.14 ± 0.26 Ma. Assuming that slip along all four faults initiated at the same time as the Petrified Spring fault yields calculated dextral fault-slip rates of 0.4 ± 0.1–0.6 ± 0.1 mm/yr, 0.4 ± 0.1–0.5 ± 0.1 mm/yr, 0.6 ± 0.1 mm/yr, and 0.3 ± 0.1 mm/yr on the four faults, respectively. Middle Miocene initiation of dextral fault slip across the GVGR overlaps with the onset of normal slip along range-bounding faults in the western Basin and Range to the north and the northern Eastern California shear zone to the south. Based on this spatial-temporal relationship, we propose that dextral fault slip across the GVGR defines a kinematic link or accommodation zone between the two regions of extension. At the time of initiation of dextral slip across the GVGR, the plate-boundary setting to the west was characterized by subduction of the Farallon plate beneath the North American plate. To account for the middle Miocene onset of extension across the Basin and Range and dextral slip in the GVGR, we hypothesize that middle Miocene trench retreat drove westward motion of the Sierra Nevada and behind it, crustal extension across the Basin and Range and NW-dextral shear within the GVGR. During the Pliocene, the plate boundary to the west changed to NW-dextral shear between the Pacific and North American plates, which drove continued dextral slip along the same faults within the GVGR because they were fortuitously aligned subparallel to plate boundary motion

Differential effects of the poly (ADP-ribose) polymerase (PARP) inhibitor NU1025 on topoisomerase I and II inhibitor cytotoxicity in L1210 cells in vitro

The potent novel poly(ADP-ribose) polymerase (PARP) inhibitor, NU1025, enhances the cytotoxicity of DNA-methylating agents and ionizing radiation by inhibiting DNA repair. We report here an investigation of the role of PARP in the cellular responses to inhibitors of topoisomerase I and II using NU1025. The cytotoxicity of the topoisomerase I inhibitor, camptothecin, was increased 2.6-fold in L1210 cells by co-incubation with NU1025. Camptothecin-induced DNA strand breaks were also increased 2.5-fold by NU1025 and exposure to camptothecin-activated PARP. In contrast, NU1025 did not increase the DNA strand breakage or cytotoxicity caused by the topoisomerase II inhibitor etoposide. Exposure to etoposide did not activate PARP even at concentrations that caused significant levels of apoptosis. Taken together, these data suggest that potentiation of camptothecin cytotoxicity by NU1025 is a direct result of increased DNA strand breakage, and that activation of PARP by camptothecin-induced DNA damage contributes to its repair and consequently cell survival. However, in L1210 cells at least, it would appear that PARP is not involved in the cellular response to etoposide-mediated DNA damage. On the basis of these data, PARP inhibitors may be potentially useful in combination with topoisomerase I inhibitor anticancer chemotherapy. © 2001 Cancer Research Campaign http://www.bjcancer.co

Quantum Chessboards in the Deuterium Molecular Ion

We present a new algorithm for vibrational control in deuterium molecules that is feasible with current experimental technology. A pump mechanism is used to create a coherent superposition of the D2+ vibrations. A short, intense infrared control pulse is applied after a chosen delay time to create selective interferences. A `chessboard' pattern of states can be realized in which a set of even- or odd-numbered vibrational states can be selectively annihilated or enhanced. A technique is proposed for experimental realization and observation of this effect using 5 fs pulses of 790 nm radiation, with intermediate intensity (5e13 W/cm2)Comment: 12 pages, 5 figure

Sinking particle fluxes from the euphotic zone over the continental slope of an eastern boundary current region

We analyze data from sediment traps and current meters moored at two locations 100 km apart over the Vancouver Island continental slope during the spring and summer of 1990. Time-series of sinking particle fluxes, major biogenic components (biogenic silica, calcium carbonate, and particulate organic carbon and nitrogen), and stable isotopic composition (δ13Corganic and δ15Ntotal) were determined on samples obtained with sequential sediment traps moored at 200–250 m depth. Associated water property data were obtained from CTD/Rosette profiles taken during trap service periods and from current meters positioned in the surface layer and near the sediment trap. These data indicate that the two locations (a southern site J and a northern site NJ) were hydrographically distinct during the investigation. At site J, we found evidence for frequent upwelling events and more variability in the upper layer water properties. The main difference in the sinking fluxes of particles between the two sites was the occurrence of a one-week event at the end of May at J that contributed about one third of the total particle flux during the sampling period. Otherwise, the total flux collected during the study and the flux of major biogenic particles were similar at both sites. Silica shells dominated the flux of particles, particularly during the spring and early summer period. At both sites, particulate organic carbon rather than calcium carbonate was the main contributor to particulate carbon fluxes. The δ13Corganic showed marked variations during the sampling period at both sites likely due to variations in the growth rate of phytoplankton and in species composition. In comparison, variations in nitrate availability appear to dominate the changes in δ15Ntotal

The multiple ionospheric probe Auroral ionospheric report

Multiple impedance and resonance probe payload for ionospheric property observation in Nike- Apache rocke

Predicting and Controlling Resolution and Surface Finish of Ceramic Objects Produced by Stereodeposition Processes

Stereodeposition techniques are well suited for the Solid Freeform Fabrication of dense ceramic components. As opposed to forming a pattern in a particle bed or polymer bath, stereodeposition processes deposit material directly onto the previously created layer. The key to stereodeposition is a material's ability to be dispensed as a fluid, yet rapidly stiffen to hold the shape of the object. This is accomplished by either solidification of a thermoplastic binder upon cooling from a melt (Fused Deposition) or by polymerization of a binder (Reactive Stereodeposition). We are developing both techniques for the production of functional ceramic and engineering polymer objects. A key issue in developing a successful stereodeposition system is controlling the rate of bead transformation from liquid to solid. Control is critical to achieving high resolution and low surface roughness of the finished product, but is made complex by the large number of parameters involved. These include binder parameters (surface tension, gelling characteristics), slurry parameters (viscosity, particle loading and size distribution), and process parameters (deposition rate, temperature). Current efforts at the University of Arizona are focused on modeling and controlling the deposition and transformation of ceramic slurries used in the Reactive Stereodeposition process.Mechanical Engineerin

Early depletion of primordial germ cells in zebrafish promotes testis formation

As complete absence of germ cells leads to sterile males in zebrafish, we explored the relationship between primordial germ cell (PGC) number and sexual development. Our results revealed dimorphic proliferation of PGCs in the early zebrafish larvae, marking the beginning of sexual differentiation. We applied morpholino-based gene knockdown and cell transplantation strategies to demonstrate that a threshold number of PGCs is required for the stability of ovarian fate. Using histology and transcriptomic analyses, we determined that zebrafish gonads are in a meiotic ovarian stage at 14 days postfertilization and identified signaling pathways supporting meiotic oocyte differentiation and eventual female fate. The development of PGC-depleted gonads appears to be restrained and delayed, suggesting that PGC number may directly regulate the variability and length of gonadal transformation and testicular differentiation in zebrafish. We propose that gonadal transformation may function as a developmental buffering mechanism to ensure the reproductive outcome

Bioink properties before, during and after 3D bioprinting

Bioprinting is a process based on additive manufacturing from materials containing living cells. These materials, often referred to as bioink, are based on cytocompatible hydrogel precursor formulations, which gel in a manner compatible with different bioprinting approaches. The bioink properties before, during and after gelation are essential for its printability, comprising such features as achievable structural resolution, shape fidelity and cell survival. However, it is the final properties of the matured bioprinted tissue construct that are crucial for the end application. During tissue formation these properties are influenced by the amount of cells present in the construct, their proliferation, migration and interaction with the material. A calibrated computational framework is able to predict the tissue development and maturation and to optimize the bioprinting input parameters such as the starting material, the initial cell loading and the construct geometry. In this contribution relevant bioink properties are reviewed and discussed on the example of most popular bioprinting approaches. The effect of cells on hydrogel processing and vice versa is highlighted. Furthermore, numerical approaches were reviewed and implemented for depicting the cellular mechanics within the hydrogel as well as for prediction of mechanical properties to achieve the desired hydrogel construct considering cell density, distribution and material-cell interaction