Intento de análisis estructural en un cuarteto de Ramón Chesa

Se intenta el análisis estructural de un texto poético de Ramón Chesa siguiendo los pasos que Jean Paul Dumont señala en el trabajo que incluye en la obra: Ensayos de semiótica poética, y que titula: «literalmente y en todos los sentidos Ensayo de análisis estructural de un cuarteto de Rimbaud.»(Ramón CHESA es un poeta desconocido al cual se ha llegado de forma casual, al conocer su obra titulada: Horas de suspiro.)Para su análisis elijo un fragmento del soneto que incluye en el libro citado y que titula: Mi hijo muerto

Detection of kinase domain mutations in BCR::ABL1 leukemia by ultra-deep sequencing of genomic DNA

The screening of the BCR::ABL1 kinase domain (KD) mutation has become a routine analysis in case of warning/failure for chronic myeloid leukemia (CML) and B-cell precursor acute lymphoblastic leukemia (ALL) Philadelphia (Ph)-positive patients. In this study, we present a novel DNA-based next-generation sequencing (NGS) methodology for KD ABL1 mutation detection and monitoring with a 1.0E−4 sensitivity. This approach was validated with a well-stablished RNA-based nested NGS method. The correlation of both techniques for the quantification of ABL1 mutations was high (Pearson r = 0.858, p < 0.001), offering DNA-DeepNGS a sensitivity of 92% and specificity of 82%. The clinical impact was studied in a cohort of 129 patients (n = 67 for CML and n = 62 for B-ALL patients). A total of 162 samples (n = 86 CML and n = 76 B-ALL) were studied. Of them, 27 out of 86 harbored mutations (6 in warning and 21 in failure) for CML, and 13 out of 76 (2 diagnostic and 11 relapse samples) did in B-ALL patients. In addition, in four cases were detected mutation despite BCR::ABL1 < 1%. In conclusion, we were able to detect KD ABL1 mutations with a 1.0E−4 sensitivity by NGS using DNA as starting material even in patients with low levels of disease.Tis project was funded in part by CRIS CANCER FOUNDATION

Detection of kinase domain mutations in BCR::ABL1 leukemia by ultra-deep sequencing of genomic DNA

The screening of the BCR::ABL1 kinase domain (KD) mutation has become a routine analysis in case of warning/failure for chronic myeloid leukemia (CML) and B-cell precursor acute lymphoblastic leukemia (ALL) Philadelphia (Ph)-positive patients. In this study, we present a novel DNA-based next-generation sequencing (NGS) methodology for KD ABL1 mutation detection and monitoring with a 1.0E-4 sensitivity. This approach was validated with a well-stablished RNA-based nested NGS method. The correlation of both techniques for the quantification of ABL1 mutations was high (Pearson r = 0.858, p < 0.001), offering DNA-DeepNGS a sensitivity of 92% and specificity of 82%. The clinical impact was studied in a cohort of 129 patients (n = 67 for CML and n = 62 for B-ALL patients). A total of 162 samples (n = 86 CML and n = 76 B-ALL) were studied. Of them, 27 out of 86 harbored mutations (6 in warning and 21 in failure) for CML, and 13 out of 76 (2 diagnostic and 11 relapse samples) did in B-ALL patients. In addition, in four cases were detected mutation despite BCR::ABL1 < 1%. In conclusion, we were able to detect KD ABL1 mutations with a 1.0E-4 sensitivity by NGS using DNA as starting material even in patients with low levels of disease

The hyper-classroom in the teaching of subjects related to Optics and Optometry

En los últimos años, las metodologías educativas se orientan hacia un mayor protagonismo del alumno, su papel como agente y su responsabilidad en el proceso de aprendizaje. Esto se puso especialmente de manifiesto en el sistema universitario español al producirse la incorporación del mismo al Espacio Europeo de Educación Superior (EEES). Las necesidades docentes para estas nuevas metodologías son diferentes a las de otras más tradicionales como la clase magistral, en la que el alumno tiene un papel pasivo como receptor de información. En el último curso, se ha sumado un factor añadido a la forma de impartir docencia, siendo este la necesidad de hacer partícipes de la clase no solo a alumnos que se encuentran en la misma aula que el profesor, sino también a otros conectados de manera online (enseñanza híbrida). La introducción de recursos y materiales físicos y digitales que permitan la adaptabilidad a situaciones y necesidades diversas y actuales es, por tanto, imprescindible, además de natural en nuestra sociedad. Para que estos cambios metodológicos puedan producirse con eficacia, deben ser acompañados de un cambio en el entorno de aprendizaje y sus recursos, ya que estos pueden ser un elemento limitante o favorecedor de la dinámica didáctica que se produce dentro del aula. La hiperaula presenta un espacio flexible que puede adaptarse a técnicas, métodos y metodologías múltiples, tanto tradicionales como recientes, así como unos recursos que permiten llevarlos a cabo.In recent years, educational methodologies are oriented towards a greater role of the student, his role as an agent and his responsibility in the learning process. This was especially evident in the Spanish university system when it was incorporated into the European Higher Education Area (EHEA). The teaching needs for these new methodologies are different from those of more traditional ones such as the master class, in which the student has a passive role as receiver of information. In the last year, an added factor has been added to the way of teaching, this being the need to involve in the class not only students who are in the same classroom as the teacher, but also others connected in a way online (hybrid teaching). The introduction of resources and physical and digital materials that allow adaptability to diverse and current situations and needs is, therefore, essential, as well as natural in our society. For these methodological changes to occur effectively, they must be accompanied by a change in the learning environment and its resources, since these can be a limiting or favorable element of the didactic dynamics that occurs within the classroom. The hyper-classroom presents a flexible space that can be adapted to multiple techniques, methods and methodologies, both traditional and recent, as well as resources that allow them to be carried out.Depto. de Optometría y VisiónFac. de Óptica y OptometríaFALSEsubmitte

Innovative open technology for an inclusive, accessible university applied to Optometry practices

Poner la última tecnología similar a la innovación en el sector de la Optometría para atención primaria a pacientes teniendo en cuanta la inserción laboral, inclusión y accesibilidad utilizando competencias digitales simples en interacción en la nube.Putting the latest technology similar to innovation in the Optometry sector for primary care for patients taking into account job placement, inclusion and accessibility using simple digital skills in interaction in the cloud.Depto. de Optometría y VisiónFac. de Óptica y OptometríaFALSEsubmitte