2,010 research outputs found
Genistein-induced mir-23b expression inhibits the growth of breast cancer cells
Aim of the study: Genistein, an isoflavonoid, plays roles in the inhibition of protein tyrosine kinase phosphorylation, induction of apoptosis, and cell differentiation in breast cancer. This study aims to induce cellular stress by exposing genistein to determine alterations of miRNA expression profiles in MCF-7 cells. Material and methods: XTT assay and trypan blue dye exclusion assays were performed to examine the cytotoxic effects of genistein treatment. Expressions of miRNAs were quantified using Real-Time Online RT-PCR. Results: The IC50 dose of genistein was 175 ΌM in MCF-7 cell, line and the cytotoxic effect of genistein was detected after 48 hours. miR-23b was found to be up-regulated 56.69 fold following the treatment of genistein. It was found that miR-23b was up-regulated for MCF-7 breast cancer cells after genistein treatment. Conclusions: Up-regulated ex-expression of miR-23b might be a putative biomarker for use in the therapy of breast cancer patients. miR-23b up-regulation might be important in terms of response to genistein. © 2015, Termedia Publishing House Ltd. All rights reserved
A well-established harmony in chaos: from isolated galaxies to galaxy clusters
The origin and evolution of galaxies are closely linked to many different physical phenomena. Among them, the most important one is the environment they reside in. Isolated and cluster member galaxies indeed are affected by different forces which affect their evolution. The main concern of this thesis is to understand such forces and how they are related to galaxy evolution. Therefore, this thesis covers various topics including black hole mass calculations, the black hole mass-stellar velocity dispersion relation, the nature of AGN emission in galaxy clusters and field galaxies, a detailed investigation of X-ray and optical galaxy overdensity phenomenon, and the dynamical processes in pre-merging galaxy clusters. Sterrewacht - OU
Dynamical history of a binary cluster: Abell 3653
We study the dynamical structure of a bimodal galaxy cluster Abell 3653 at z = 0.1089 using optical and X-ray data. Observations include archival data from the Anglo-Australian Telescope, X-ray observatories XMMâNewton and Chandra. We draw a global picture for A3653 using galaxy density, X-ray luminosity and temperature maps. The galaxy distribution has a regular morphological shape at the 3âMpc size. The galaxy density map shows an elongation in the eastâwest direction, which perfectly aligns with the extended diffuse X-ray emission. We detect two dominant groups around the two brightest cluster galaxies (BCGs). BCG1 (z = 0.1099) can be associated with the main cluster A3653E, and a foreground subcluster A3653W is concentrated at BCG2 (z = 0.1075). Both X-ray peaks are dislocated from the BCGs by âŒ35âkpc, which suggest an ongoing merger process. We measure the subcluster gas temperatures of 4.67 and 3.66âkeV, respectively. Two-body dynamical analysis shows that A3653E and A3653W are very likely gravitationally bound (93.5âperâcent probability). The highly favoured scenario suggests that the two subclusters have a mass ratio of 1.4 and are colliding close to the plane of sky (α = 17.61°) at 2400âkmâsâ1, and will undergo core passage in 380âMyr. The temperature map also significantly shows a shock-heated gas (6.16âkeV) between the subclusters, which confirms the supersonic infalling scenario.Large scale structure and cosmologyHigh Energy Astrophysic
Analysis of ice-sheet temperature profiles from low-frequency airborne remote sensing
Abstract
Ice internal temperature and basal geothermal heat flux (GHF) are analyzed along a study line in northwestern Greenland. The temperatures were obtained from a previously reported inversion of airborne microwave brightness-temperature spectra. The temperatures vary slowly through the upper ice sheet and more rapidly near the base increasing from ~259 K near Camp Century to values near the melting point near NorthGRIP. The flow-law rate factor is computed from temperature data and analytic expressions. The rate factor increases from ~1 Ă 10â8 to 8 Ă 10â8 kPaâ3 aâ1 along the line. A laminar flow model combined with the depth-dependent rate factor is used to estimate horizontal velocity. The modeled surface velocities are about a factor of 10 less than interferometric synthetic aperture radar (InSAR) surface velocities. The laminar velocities are fitted to the InSAR velocities through a factor of 8 enhancement of the rate factor for the lower 25% of the column. GHF values retrieved from the brightness temperature spectra increase from ~55 to 84 mW mâ2 from Camp Century to NorthGRIP. A strain heating correction improves agreement with other geophysical datasets near Camp Century and NEEM but differ by ~15 mW mâ2 in the central portion of the profile
Volumetric evaluation of fat in the renal sinus in normal subjects using stereological method on computed tomography images and its relationship with body composition
Background: The aim of the study was: to describe a simple, accurate and practical technique for estimating the volume of adipose tissue within the renal sinus (RS) using stereological method on computed tomography (CT) images; to establish a population database for volume of fat within the RS from 21 to 80 years of age; to investigate the effect of age, gender, body mass index (BMI) and abdominal diameters on RS fat volume in normal subjects.Materials and methods: We retrospectively reviewed abdominal CT examinations of 240 patients without renal pathology between the ages of 21 and 80 years. There were 6 groups of patients, with 40 patients for each decade.Results: RS fat volumes in the left and right kidney were 5.70 ± 2.87 cm3 and 4.15 ± 2.39 cm3, respectively, in males and 3.51 ± 2.67 cm3 and 2.49 ± 2.16 cm3, respectively, in females. RS fat volume and age were positively correlated for both kidneys (left: r = 0.46; right: r = 0.44; p < 0.001, both), though it appeared to decline after age 70.Conclusions: Quantitative data may allow clinicians to better estimate the age-related RS fat volume changes and help them in decision making
Probiotic Lactobacillus paracasei effect on cariogenic bacterial flora
Lactobacillus paracasei has been demonstrated to inhibit the growth of many pathogenic microbes such as Streptococcus mutans, in vitro. However, its clinical application remains unclear. Here, we examined whether a novel probiotic L. paracasei GMNL-33 may reduce the caries-associated salivary microbial counts in healthy adults. Seventy-eight subjects (aged 20 to 26) had completed this double-blinded, randomized, placebo-controlled study. A probiotic/test (nâ=â42) and a control group (nâ=â36) took a L. paracasei GMNL-33 and a placebo oral tablet three times per day for 2Â weeks, respectively. Bacterial counts of salivary S. mutans, lactobacilli, and salivary buffer capacity were measured with chair-side kits at the beginning (T1), the completion (T2) of medication, and 2Â weeks after medication (T3). The results did not show differences in the counts of S. mutans and lactobacilli between probiotic and control groups at T1, T2, and T3. Nevertheless, within the probiotic group, an interesting probiotic effect was noticed. Between T1 and T2, no inhibitory effect against S. mutans was observed. However, a significant count reduction in the salivary S. mutans was detected between T2 and T3 (pâ=â0.016). Thus, a 2-week period of medication via oral administration route may be needed for L. paracasei GMNL-33 to be effective in the probiotic action
Robotic right ventricle is a biohybrid platform that simulates right ventricular function in (patho)physiological conditions and intervention
Role of a Novel Heparanase Inhibitor on the Balance between Apoptosis and Autophagy in U87 Human Glioblastoma Cells
Background: Heparanase (HPSE) is an endo-& beta;-glucuronidase that cleaves heparan sulfate side chains, leading to the disassembly of the extracellular matrix, facilitating cell invasion and metastasis dissemination. In this research, we investigated the role of a new HPSE inhibitor, RDS 3337, in the regulation of the autophagic process and the balance between apoptosis and autophagy in U87 glioblastoma cells. Methods: After treatment with RDS 3337, cell lysates were analyzed for autophagy and apoptosis-related proteins by Western blot. Results: We observed, firstly, that LC3II expression increased in U87 cells incubated with RDS 3337, together with a significant increase of p62/SQSTM1 levels, indicating that RDS 3337 could act through the inhibition of autophagic-lysosomal flux of LC3-II, thereby leading to accumulation of lipidated LC3-II form. Conversely, the suppression of autophagic flux could activate apoptosis mechanisms, as revealed by the activation of caspase 3, the increased level of cleaved Parp1, and DNA fragmentation. Conclusions: These findings support the notion that HPSE promotes autophagy, providing evidence that RDS 3337 blocks autophagic flux. It indicates a role for HPSE inhibitors in the balance between apoptosis and autophagy in U87 human glioblastoma cells, suggesting a potential role for this new class of compounds in the control of tumor growth progression
Heterogeneous Delays in Neural Networks
We investigate heterogeneous coupling delays in complex networks of excitable
elements described by the FitzHugh-Nagumo model. The effects of discrete as
well as of uni- and bimodal continuous distributions are studied with a focus
on different topologies, i.e., regular, small-world, and random networks. In
the case of two discrete delay times resonance effects play a major role:
Depending on the ratio of the delay times, various characteristic spiking
scenarios, such as coherent or asynchronous spiking, arise. For continuous
delay distributions different dynamical patterns emerge depending on the width
of the distribution. For small distribution widths, we find highly synchronized
spiking, while for intermediate widths only spiking with low degree of
synchrony persists, which is associated with traveling disruptions, partial
amplitude death, or subnetwork synchronization, depending sensitively on the
network topology. If the inhomogeneity of the coupling delays becomes too
large, global amplitude death is induced
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