Unbiased Functional Proteomics Strategy for Protein Kinase Inhibitor Validation and Identification of bona fide Protein Kinase Substrates: Application to Identification of EEF1D as a Substrate for CK2

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A global action agenda for turning the tide on fatty liver disease

Background and Aims: Fatty liver disease is a major public health threat due to its very high prevalence and related morbidity and mortality. Focused and dedicated interventions are urgently needed to target disease prevention, treatment, and care. Approach and Results: We developed an aligned, prioritized action agenda for the global fatty liver disease community of practice. Following a Delphi methodology over 2 rounds, a large panel (R1 n = 344, R2 n = 288) reviewed the action priorities using Qualtrics XM, indicating agreement using a 4-point Likert-scale and providing written feedback. Priorities were revised between rounds, and in R2, panelists also ranked the priorities within 6 domains: epidemiology, treatment and care, models of care, education and awareness, patient and community perspectives, and leadership and public health policy. The consensus fatty liver disease action agenda encompasses 29 priorities. In R2, the mean percentage of “agree” responses was 82.4%, with all individual priorities having at least a super-majority of agreement (> 66.7% “agree”). The highest-ranked action priorities included collaboration between liver specialists and primary care doctors on early diagnosis, action to address the needs of people living with multiple morbidities, and the incorporation of fatty liver disease into relevant non-communicable disease strategies and guidance. Conclusions: This consensus-driven multidisciplinary fatty liver disease action agenda developed by care providers, clinical researchers, and public health and policy experts provides a path to reduce the prevalence of fatty liver disease and improve health outcomes. To implement this agenda, concerted efforts will be needed at the global, regional, and national levels.publishedVersio

A global research priority agenda to advance public health responses to fatty liver disease

Background & aims An estimated 38% of adults worldwide have non-alcoholic fatty liver disease (NAFLD). From individual impacts to widespread public health and economic consequences, the implications of this disease are profound. This study aimed to develop an aligned, prioritised fatty liver disease research agenda for the global health community. Methods Nine co-chairs drafted initial research priorities, subsequently reviewed by 40 core authors and debated during a three-day in-person meeting. Following a Delphi methodology, over two rounds, a large panel (R1 n = 344, R2 n = 288) reviewed the priorities, via Qualtrics XM, indicating agreement using a four-point Likert-scale and providing written feedback. The core group revised the draft priorities between rounds. In R2, panellists also ranked the priorities within six domains: epidemiology, models of care, treatment and care, education and awareness, patient and community perspectives, and leadership and public health policy. Results The consensus-built fatty liver disease research agenda encompasses 28 priorities. The mean percentage of ‘agree’ responses increased from 78.3 in R1 to 81.1 in R2. Five priorities received unanimous combined agreement (‘agree’ + ‘somewhat agree’); the remaining 23 priorities had >90% combined agreement. While all but one of the priorities exhibited at least a super-majority of agreement (>66.7% ‘agree’), 13 priorities had 90% combined agreement. Conclusions Adopting this multidisciplinary consensus-built research priorities agenda can deliver a step-change in addressing fatty liver disease, mitigating against its individual and societal harms and proactively altering its natural history through prevention, identification, treatment, and care. This agenda should catalyse the global health community’s efforts to advance and accelerate responses to this widespread and fast-growing public health threat. Impact and implications An estimated 38% of adults and 13% of children and adolescents worldwide have fatty liver disease, making it the most prevalent liver disease in history. Despite substantial scientific progress in the past three decades, the burden continues to grow, with an urgent need to advance understanding of how to prevent, manage, and treat the disease. Through a global consensus process, a multidisciplinary group agreed on 28 research priorities covering a broad range of themes, from disease burden, treatment, and health system responses to awareness and policy. The findings have relevance for clinical and non-clinical researchers as well as funders working on fatty liver disease and non-communicable diseases more broadly, setting out a prioritised, ranked research agenda for turning the tide on this fast-growing public health threat

PREPARACIÓN DE PERLAS DE ALGINATO DE CALCIO CON PROPIEDADES MAGNÉTICAS Y SU APLICACIÓN EN LA ADSORCIÓN DE Cu (II)

It has been obtained bead of calcium alginate wit micro-particles of magnetite. The optimum pH for adsorption of Cu (II) lie between 4,5 and 5. From the study of the kinetic of adsorption was determined that the thermodynamic equilibrium was reached after 200 min. The adsorption data fitted well with the Langmuir model and showed the maximum adsorption capacity q(max)= 57,47 mg/g and b = 0,0784.Se han obtenido perlas de alginato de calcio con propiedades magnéticas. El pH óptimo para la adsorción del ion Cu(II) se encuentra en el intervalo de 4,5 a 5. De los datos sobre la cinética del proceso de adsorción se ha determinado que el equilibrio termodinámico se alcanza después de 200 minutos del inicio del proceso. Los datos experimentales de la adsorción se describen por el modelo de Langmuir y se ha determinado que la máxima capacidad de adsorción es q(max)= 57,47 mg/g y b = 0,0784

PERLAS DE QUITOSANO CON PARTÍCULAS MAGNÉTICAS Y SU APLICACIÓN EN LA ADSORCIÓN DE IONES DE Cu (II)

The objective of this work is to prepare chitosan beads with magnetic properties for use in the adsorption of Cu (II). Through a process reported in the literature have been obtained with micro chitosan beads magnetite particles of2,5 mm diameter in the wet state and diameter of 0,8 mm when dry. Subsequently chitosan beads adsorption used for Cu (II), in a batch system, determining which optimum pH is in the range of 4.7 to 5.2. The study of the kinetics of the adsorption process showed that thermodynamic equilibrium is attained 250 min before starting the process. The experimental data described adsorption by the Langmuir model and found that the maximum adsorption capacity is qmax = 235,84 mg/g constant value Langmuir, b = 0,00442El objetivo del trabajo es preparar perlas de quitosano con propiedades magnéticas para utilizarlas en la adsorción de Cu(II). Mediante un proceso reportado en la literatura se han obtenido perlas de quitosano con micro partículas de magnetita de 2,5 mm de diámetro en estado húmedo, y de 0,8 mm de diámetro en estado seco. Posteriormente las perlas de quitosano se emplean para la adosrción de Cu(II), en un sistema batch, determinándose que pH óptimo se encuentra en el intervalo de 4,7 a 5,2. El estudio de la cinéticadel proceso de adsorción demostró que el equilibrio termodinámico se alcanza antes de 250 min deiniciado el proceso. Los datos experimentales de la adsorción se describen por el modelo de Langmuir yse ha determinado que la máxima capacidad de adsorción es qmax=235,84 mg/g y valor de la constantede Langmuir, b=0,00442

ELEMENTOS FLORÍSTICOS DEL HUMEDAL LAGUNA MANCOCHE, PROVINCIA CHEPÉN, REGIÓN LA LIBERTAD, PERÚ

Se registró preliminarmente a las especies vegetales y evidenció el estado de conservación del humedal Laguna Mancoche, provincia Chepén, región La Libertad, Perú (120 msnm; 7°13´01.24” S 79°25´31.09” O), durante visitas periódicas irregulares entre 2016 y 2021. Es un ecosistema no referenciado en estudios académicos. La flora estuvo constituida por 26 especies distribuidas en 22 géneros y 15 familias. Se enfatiza la predominancia de Typha angustifolia L. (Typhaceae). A pesar que es considerado un atractivo turístico, como fuente de recursos naturales para la confección de artículos domésticos y alimentación, y su manantial sirve para uso agrícola y doméstico, se encuentra descuidado. El mal estado de conservación se debe a que los pobladores arrojan basura y desmonte a su lecho, queman los tifales, totorales y juncales, talan la flora ribereña, crían animales domésticos en sus alrededores, etc.; así mismo, está perdiendo su extensión por la ampliación de la frontera agrícola. Actualmente, este ecosistema cuenta con unas 8 hectáreas aproximadamente