Potential of the Senegal sole Solea senegalensis Kaup, 1858 for marine aquaculture diversification

The potential of Senegal sole Solea senegalensis Kaup, 1858 for marine aquaculture diversification is analysed. An initial historic review attempts to explain the current situation of this sole species's aquaculture. Recent advances are then assessed, highlighting progress in captive reproduction techniques, even though a great deal of scientific knowledge is still lacking. Ongrowing of sole has been successfully conducted in several instances, but progress is limited by the excessive occurrence of opportunistic diseases. These may be, at present, a consequence of suboptimal husbandry conditions which make sole unable to achieve a sufficiently adequate physiological status to generate proper resistance.Se analizan las posibilidades que el lenguado senegalés Solea senegalensis Kaup, 1858 presenta para diversificar la acuicultura marina. Se trata de una revisión en la que se describe la evolución del cultivo de esta especie como paso previo para comprender su situación actual. A continuación, se valoran los avances más recientes, observándose cómo la zootecnia de la reproducción en cautividad va progresando en respuesta al esfuerzo investigador invertido, aunque todavía son numerosas las carencias de su conocimiento científico de base. El engorde de lenguados ha producido en varios casos resultados satisfactorios, sin embargo, su avance se ve limitado por una excesiva incidencia de patologías oportunistas, la mayoría de las cuales son consecuencia de condiciones de procedimiento que no favorecen el bienestar fisiológico suficiente para hacerles frente.Instituto Español de Oceanografí

Efecto de pretratamientos desvulcanizantes y de aditivos en materiales compuestos con matriz de HDPE y neumáticos fuera de uso

La gestión de los neumáticos al final de su ciclo de vidaprecisa soluciones para potenciar su reutilización y reciclado.Un paso previo consiste en la pulverización de losneumáticos por parte de empresas especializadas. La incorporaciónde este granulado como refuerzo en matricestermoplásticas se ha propuesto como método de valorizacióndel polvo de neumático. Este sistema se ve limitadopor la incompatibilidad entre matriz y refuerzo. Recientemente,un nuevo polvo de neumático, denominado comercialmente “desvulcanizado” ha aparecido en el mercado.En este artículo se comparan materiales compuestosformulados con polvo de neumático desvulcanizado conlos obtenidos con el mismo material sin tratamiento enmatriz de polietileno de alta densidad. También se estudiael efecto de distintos métodos de mezclado, del uso deaditivos dispersantes y del tamaño de partícula

A canine leishmaniasis pilot survey in an emerging focus of visceral leishmaniasis: Posadas (Misiones, Argentina)

<p>Abstract</p> <p>Background</p> <p>An increasing number of reports are calling our attention to the worldwide spread of leishmaniasis. The urbanization of zoonotic visceral leishmaniasis (VL) has been observed in different South American countries, due to changes in demographic and ecological factors. In May 2006, VL was detected for the first time in the city of Posadas (Misiones, Argentina). This event encouraged us to conduct a clinical and parasitological pilot survey on domestic dogs from Posadas to identify their potential role as reservoirs for the disease.</p> <p>Methods</p> <p>One hundred and ten dogs from the city of Posadas were included in the study. They were selected based on convenience and availability. All dogs underwent clinical examination. Symptomatology related to canine leishmaniasis was recorded, and peripheral blood and lymph node aspirates were collected. Anti-<it>Leishmania </it>antibodies were detected using rK39-immunocromatographic tests and IFAT. Parasite detection was based on peripheral blood and lymph node aspirate PCR targeting the <it>SSUrRNA </it>gene. Molecular typing was addressed by DNA sequence analysis of the PCR products obtained by <it>SSUrRNA </it>and ITS-1 PCR.</p> <p>Results</p> <p>According to clinical examination, 69.1% (76/110) of the dogs presented symptoms compatible with canine leishmaniasis. Serological analyses were positive for 43.6% (48/110) of the dogs and parasite DNA was detected in 47.3% (52/110). A total of 63 dogs (57.3%) were positive by serology and/or PCR. Molecular typing identified <it>Leishmania infantum </it>(syn. <it>Leishmania chagasi</it>) as the causative agent.</p> <p>Conclusions</p> <p>This work confirms recent findings which revealed the presence of <it>Lutzomyia longipalpis</it>, the vector of <it>L. infantum </it>in this area of South America. This new VL focus could be well established, and further work is needed to ascertain its magnitude and to prevent further human VL cases.</p

Probiotic supplementation influences the diversity of the intestinal microbiota during early stages of farmed Senegalese sole (Solea senegalensis, Kaup, 1858)

Ingestion of bacteria at early stages results in establishment of a primary intestinal microbiota which likely undergoes several stages along fish life. The role of this intestinal microbiota regulating body functions is crucial for larval development. Probiotics have been proved to modulate this microbiota and exert antagonistic effects against fish pathogens. In the present study, we aimed to determine bacterial diversity along different developmental stages of farmed Senegalese sole (Solea senegalensis) after feeding probiotic (Shewanella putrefaciens Pdp11) supplemented diet for a short period (10–30 days after hatching, DAH). Intestinal lumen contents of sole larvae fed control and probiotic diets were collected at 23, 56, 87, and 119 DAH and DNA was amplified using 16S rDNA bacterial domain-specific primers. Amplicons obtained were separated by denaturing gradient gel electrophoresis (DGGE), cloned, and resulting sequences compared to sequences in GenBank. Results suggest that Shewanella putrefaciens Pdp11 induces a modulation of the dominant bacterial taxa of the intestinal microbiota from 23 DAH. DGGE patterns of larvae fed the probiotic diet showed a core of bands related to Lactobacillus helveticus, Pseudomonas acephalitica, Vibrio parahaemolyticus,and Shewanella genus, together with increased Vibri o genus presence. In addition, decreased number of clones related to Photobacterium damselae subsp piscicida at 23 and 56 DAH was observed in probiotic-fed larvae. A band corresponding to Shewanella putrefaciens Pdp11 was sequenced as predominant from 23 to 119 DAH samples, confirming the colonization by the probiotics. Microbiota modulation obtained via probiotics addition emerges as an effective tool to improve Solea senegalensis larviculture.En prens

Predicting the onset and persistence of episodes of depression in primary health care. The predictD-Spain study: Methodology

Background: The effects of putative risk factors on the onset and/or persistence of depression remain unclear. We aim to develop comprehensive models to predict the onset and persistence of episodes of depression in primary care. Here we explain the general methodology of the predictD-Spain study and evaluate the reliability of the questionnaires used. Methods: This is a prospective cohort study. A systematic random sample of general practice attendees aged 18 to 75 has been recruited in seven Spanish provinces. Depression is being measured with the CIDI at baseline, and at 6, 12, 24 and 36 months. A set of individual, environmental, genetic, professional and organizational risk factors are to be assessed at each follow-up point. In a separate reliability study, a proportional random sample of 401 participants completed the test-retest (251 researcher-administered and 150 self-administered) between October 2005 and February 2006. We have also checked 118,398 items for data entry from a random sample of 480 patients stratified by province. Results: All items and questionnaires had good test-retest reliability for both methods of administration, except for the use of recreational drugs over the previous six months. Cronbach's alphas were good and their factorial analyses coherent for the three scales evaluated (social support from family and friends, dissatisfaction with paid work, and dissatisfaction with unpaid work). There were 191 (0.16%) data entry errors. Conclusion: The items and questionnaires were reliable and data quality control was excellent. When we eventually obtain our risk index for the onset and persistence of depression, we will be able to determine the individual risk of each patient evaluated in primary health care.The research in Spain was funded by grants from the Spanish Ministry of Health (grant FIS references: PI04/1980, PI0/41771, PI04/2450, and PI06/1442), Andalusian Council of Health (grant references: 05/403, 06/278 and 08/0194), and the Spanish Ministry of Education and Science (grant reference SAF 2006/07192). The Malaga sample, as part of the predictD-International study, was also funded by a grant from The European Commission (reference QL4-CT2002-00683)

Massive transcriptome sequencing in sole

&nbsp; The rapid development of Next Generation Sequencing (NGS) techniques has transformed the way genomic research and characterization of genomes is done today. They can be applied to a wide variety of biological questions, from the sequencing of complete eukaryotic and prokaryotic genomes and transcriptomes, genome-scale analysis of DNA&ndash;protein interactions to metapopulation studies. Ultra-high throughput sequencing of the transcriptome (RNA-seq) has become a powerful and attractive alternative technology for gene discovery, transcript quantification and marker discovery. In this presentation, we focus on the use of NGS in sole transcriptomics. Particularly, we have performed two 454 runs for transcriptome analysis in Senegalese sole. In the first run, we tried to evaluate the defence mechanisms against pathogens throughout a better knowledge of the transcriptome of five immunostimulated organs (a pool of Head kidney, spleen, thymus, brain, gills).Presentaci&oacute;n oral en Workshop The Cultivation of Soles V. 5-7 Abril, 2011. Faro (Portugal).</p

Intensive care nurses on social media: An exploration of knowledge exchange on an intensive care virtual community of practice

Aims and objectives To explore the nature of knowledge exchange on a multi‐disciplinary Australasian intensive care virtual community of practice, “ICUConnect.” Background Current organisational structures and cultures constrain the social networks of healthcare professionals, limiting access to contemporary best practice knowledge. While virtual communities can facilitate knowledge and clinical expertise exchange in professional networks, their effectiveness has not been established. Design A sequential mixed‐methods design with a quantitative core and qualitative supplementary component was used to explore the content of discussions from an intensive care virtual community. SRQR has been used to report this study. Methods Email archives of an intensive care listserv (2003–2013) were mined using a two‐stage sampling technique to identify discussion threads (with \u3e2 posts) concerning ventilator or airway practices (cluster) and two sets of 20 threads (stratified across years). Summative content analysis was used to examine both manifest and latent content. Results Forty threads containing 326 emails posted by 133 individuals from 80 organisations were analysed. Nurses contributed 68% (55% were in clinical leadership roles) and physicians 27%. Three subject areas were identified: clinical practices (71%); equipment (23%); and clinical governance (6%). “Knowledge‐requested” and “knowledge‐supplied” posts were categorised as follows: experiential and explicit (33% and 16%, respectively); experiential (27% and 35%); or explicit (40% and 17%). Knowledge supplied was also categorised as “know‐how” (20%); “know‐why” (5%) or “no knowledge” exchanged (6%). The central construct of virtual community work was identified with six elements that facilitated participation and knowledge exchange including: (a) the discussion thread; (b) sharing of artefacts; (c) community; (d) cordiality; (e) maven work; and (f) promotion of the VC. Members asked questions to benchmark their practice, while those who answered were focused on ensuring that best practices were delivered. Conclusions ICUConnect reflected characteristics of a virtual community of practice, enabling key benefits for members and the broader Australasian intensive care community, especially access to best practice knowledge from clinical experts. Relevance to clinical practice This study demonstrated that a practice‐based VC can function effectively as a VCoP to establish an effective professional network where members have access to up‐to‐date best practice knowledge. Healthcare organisations could leverage VCs to support the professional development of HCPs and ensure that local clinical practices are based on contemporaneous knowledge. Participation by nurses in these communities facilitates individual professional development and access to important clinical knowledge and expertise, and ultimately reinforcing the unique position of nursing in delivering effective, consistent high‐quality patient care