29 research outputs found
Marine mammals and Good Environmental Status: Science, Policy and Society; Challenges and Opportunities
The Marine Strategy Framework Directive has become the key instrument for marine conservation in European seas. We review its implementation, focusing on cetacean biodiversity, using
the examples of Spain and the Regional Seas Convention, OSPAR. The MSFD has been widely criticised for legal vagueness, lack of coordination,
uncertainty about funding, and poor governance; its
future role within EU Integrated Maritime Policy
remains unclear. Nevertheless, the first stages of the
process have run broadly to schedule: current status,
environmental objectives and indicators have been
described and the design of monitoring programmes is in progress, drawing on experience with other environmental
legislation. The MSFD is now entering its
critical phase, with lack of funding for monitoring,
limited scope for management interventions, and
uncertainty about how conservation objectives will
be reconciled with the needs of other marine and
maritime sectors, being among the main concerns.
Clarity in governance, about the roles of the EU,
Member States, Regional Seas Conventions and
stakeholders, is needed to ensure success. However,
even if (as seems likely) good environmental status
cannot be achieved by 2020, significant steps will have been taken to place environmental sustainability
centre-stage in the development of Integrated Maritime
Policy for EU seas.Postprin
Anal and oral human papillomavirus (HPV) infection in HIV-infected subjects in northern Italy: a longitudinal cohort study among men who have sex with men
<p>Abstract</p> <p>Background</p> <p>A study including 166 subjects was performed to investigate the frequency and persistence over a 6-month interval of concurrent oral and anal Human Papillomavirus (HPV) infections in Human Immunodeficiency Virus (HIV)-infected men who have sex with men (MSM).</p> <p>Methods</p> <p>Patients with no previously documented HPV-related anogenital lesion/disease were recruited to participate in a longitudinal study. Polymerase chain reaction (PCR) was performed to detect HPV from oral and anal swabs and to detect Human Herpes Virus 8 (HHV-8) DNA in saliva on 2 separate specimen series, one collected at baseline and the other collected 6 months later. A multivariate logistic analysis was performed using anal HPV infection as the dependent variable versus a set of covariates: age, HIV plasma viral load, CD4+ count, hepatitis B virus (HBV) serology, hepatitis C virus (HCV) serology, syphilis serology and HHV-8 viral shedding. A stepwise elimination of covariates with a p-value > 0.1 was performed.</p> <p>Results</p> <p>The overall prevalence of HPV did not vary significantly between the baseline and the follow-up, either in the oral (20.1 and 21.3%, respectively) or the anal specimens (88.6 and 86.3%). The prevalence of high-risk (HR) genotypes among the HPV-positive specimens was similar in the oral and anal infections (mean values 24.3% and 20.9%). Among 68 patients with either a HR, low-risk (LR) or undetermined genotype at baseline, 75% had persistent HPV and the persistence rates were 71.4% in HR infections and 76.7% in LR infections. There was a lack of genotype concordance between oral and anal HPV samples. The prevalence of HR HPV in anus appeared to be higher in the younger patients, peaking (> 25%) in the 43-50 years age group. A decrease of the high level of anal prevalence of all genotypes of HPV in the patients > 50 years was evident. HHV-8 oral shedding was positively related to HPV anal infection (p = 0.0046). A significant correlation was found between the persistence of HHV-8 shedding and HIV viral load by logistic bivariate analysis (Odds Ratio of HHV-8 persistence for 1-log increase of HIV viral load = 1.725 ± 0.397, p = 0.018).</p> <p>Conclusions</p> <p>A high prevalence of HPV infection was found in our cohort of HIV-infected MSM, with a negative correlation between anal HPV infection and CD4 cell count.</p
Is salinity the main ecologic factor that shapes the distribution of two endemic Mediterranean plant species of the genus Gypsophila?
The final publication is available at Springer via http://dx.doi.org/10.1007/s11104-014-2218-2Aims Responses to salt stress of two Gypsophila species that share territory, but with different ecological optima and distribution ranges, were analysed. G. struthium is a regionally dominant Iberian endemic gypsophyte, whereas G. tomentosa is a narrow endemic reported as halophyte. Theworking hypothesis is that salt tolerance shapes the presence of these species in their specific habitats. Methods Taking a multidisciplinary approach, we assessed the soil characteristics and vegetation structure at the sampling site, seed germination and seedling development, growth and flowering, synthesis of proline and cation accumulation under artificial conditions of increasing salt stress and effect of PEG on germination
and seedling development. Results Soil salinity was low at the all sampling points where the two species grow, but moisture was higher in the area of G. tomentosa. Differences were found in the species salt and drought tolerance. The different parameters tested did not show a clear pattern indicating the main role of salt tolerance in plant distribution. Conclusions G. tomentosa cannot be considered a true halophyte as previously reported because it is unable to complete its life cycle under salinity. The presence of G. tomentosa in habitats bordering salt marshes is a strategy to avoid plant competition and extreme water
stressSoriano, P.; Moruno ManchĂłn, JF.; Boscaiu Neagu, MT.; Vicente Meana, Ă.; Hurtado, A.; Llinares Palacios, JV.; Estrelles, E. (2014). Is salinity the main ecologic factor that shapes the distribution of two endemic Mediterranean plant species of the genus Gypsophila?. Plant and Soil. 384(1-2):363-379. doi:10.1007/s11104-014-2218-2S3633793841-2Alonso MA (1996) Flora y vegetaciĂłn del Valle de Villena (Alicante). Instituto de Cultura Juan Gil-Albert, AlicanteAlvarado JJ, Ruiz JM, LĂłpez-Cantarero I, Molero J, Romero L (2000) Nitrogen metabolism in five plant species characteristic of gypsiferous soils. Plant Physiol 156:612â616Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59:206â216Ashraf MY (2009) Salt tolerance mechanisms in some halophytes from Saudi Arabia and Egypt. Res J Agric Biol Sci 5:191â206Bates LS, Waldren RP, Tear LD (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205â207Ben-Gal A, Neori-Borochov H, Yermiyahu U, Shani U (2009) Is osmotic potential a more appropriate property than electrical conductivity for evaluating whole plant response to salinity? Environ Exp Bot 65:232â237Biondi E (2011) Phytosociology today: Methodological and conceptual evolution. Plant Biosyst 145:19â29Boscaiu M, Bautista I, LidĂłn A, Llinares J, Lull C, Donat P, Mayoral O, Vicente O (2013a) Environmental-dependent proline accumulation in plants living on gypsum soils. Acta Physiol Plant 35:2193â2204Boscaiu M, Llul C, Llinares J, Vicente O, Boira H (2013b) Proline as a biochemical marker in relation to the ecology of two halophytic Juncus species. J Plant Ecol 6:177â186Bradford KJ (1990) A water relations analysis of seed germination rates. Plant Physiol 94:840â849Breckle SW (1999) Halophytic and gypsophytic vegetation of the Ebro-Basin at Los Monegros. In: Melic A, Blasco-Zumeta J (eds) Manifiesto cientĂfico por Los Monegros, vol 24, Bol. SEA., pp 101â104Brenchley JL, Probert RJ (1998) Seed germination responses to some environmental factors in the sea grass Zoostera capricorni from eastern Australia. Aquat Bot 62:177â188Cañadas EM, Ballesteros M, Valle F, Lorite J (2013) Does gypsum influence seed germination? Turk J Bot 38:141â147Chen Z, Cuin TA, Zhou M et al (2007) Compatible solute accumulation and stress-mitigating effects in barley genotypes contrasting in their salt tolerance. J Exp Bot 58:4245â4255Chutipaijit S, Cha-Um S, Sompornailin K (2009) Differential accumulation of proline and flavonoids in Indica rice varieties against salinity. Pak J Bot 41:2497â2506Cushman JC (2001) Osmoregulation in plants: implications for agriculture. Am Zool 41:758â769Debussche M, Thompson JD (2003) Habitat differentiation between two closely related Mediterranean plant species, the endemic Cyclamen balearicum and the widespread C. repandum. Acta Oecol 24:35â45Eskandari H, Kazemi K (2011) Germination and seedling properties of different wheat cultivars under salinity conditions. Not Sci Biol 3:130â134FAO (2006) Guidelines for soil descriptions, 5th edn. Food and Agricultural Organization of United Nation, RomeFerrandis P, Herranz JM, Copete MA (2005) CaracterizaciĂłn florĂstica y edĂĄfica de las estepas yesosas de Castilla-La Mancha. Invest Agrar Sist Recur For 14:195â216Flowers TJ, Hall JL (1978) Salt tolerance in Suaeda maritima (L.) Dum. The effect of sodium chloride on growth and soluble enzymes in a comparative study with Pisum sativum L. J Exp Bot 23:310â321Flowers TJ, Colmer TD (2008) Salinity tolerance in halophytes. New Phytol 179:945â963Flowers TJ, Hajibagheri MA, Clipson NJW (1986) Halophytes. Q Rev Biol 61:313â335GarcĂa-Fuentes A, Salazar C, Torres JA, Cano E, Valle F (2001) Review of communities of Lygeum spartum L. in the south-eastern Iberian Peninsula (western Mediterranean). J Arid Environ 48:323â339GĂ©hu JM (2006) Dictionnaire de Sociologie et SynĂ©cologie VĂ©gĂ©tales. J. Cramer, Berlin-Stuttgart, p 899GĂ©hu JM (2011) On the opportunity to celebrate the centenary of modern phytosociology in 2010. Plant Biosyst 145(suppl):4â8Ghassemi F, Jakeman AJ, Nix HA (1995) Salinisation of land and water resources: human causes, extent, management and case studies. Canberra, Australia. CAB International, The Australian National University, WallingfordGrigore MN, Boscaiu M, Vicente O (2011) Assessment of the relevance of osmolyte biosynthesis for salt tolerance of halophytes under natural conditions. Eur J Plant Sci Biotech 5:12â19Grigore MN, Villanueva M, Boscaiu M, Vicente O (2012a) Do halophytes really require salts for their growth and development? An experimental approach mitigation of salt stress-induced inhibition of Plantago crassifolia reproductive development by supplemental calcium or magnesium. Not Sci Biol 4:23â29Grigore MN, Boscaiu M, Llinares J, Vicente O (2012b) Mitigation of salt stressed-induced Inhibition of Plantago crassifolia reproductive development by supplemental calcium or magnesium. Not Bot Horti Agrobo 40:58â66Hare PD, Cress WA (1997) Metabolic implications of stress-induced proline accumulation in plants. Plant Growth Regul 21:79â102Ishikawa SI, Kachi N (2000) Differential salt tolerance of two Artemisia species growing in contrasting coastal habitats. Ecol Res 15:241â247Kebreab E, Murdoch AJ (1999) Modelling the effects of water stress and temperature on germination rate of Orobanche aegyptiaca seeds. J Exp Bot 50:655â664Khan MA (2002) Halophyte seed germination: Success and Pitfalls. In: Hegazi AM, El-Shaer HM, El-Demerdashe S et al (eds) International symposium on optimum resource utilization in salt affected ecosystems in arid and semi arid regions. Desert Research Centre, Cairo, pp 346â358Khan MA, Gul B, Weber DJ (2000) Germination responses of Salicornia rubra to temperature and salinity. J Arid Environ 45:207â214Khan A, Rayner GD (2003) Robustness to non-normality of common tests for the many-sample location problem. J Appl Math Decis Sci 7:187â206LidĂłn A, Boscaiu M, Collado F, Vicente O (2009) Soil requirements of three salt tolerant, endemic species from south-east Spain. Not Bot Horti Agrobo 37:64â70LĂłpez GonzĂĄlez G (1990) Gypsohila L. In: Castroviejo S, LaĂnz M, LĂłpez G et al (eds) Flora IbĂ©rica 2. Real JardĂn BotĂĄnico, Madrid, pp 408â415Lutts S, Kinet JM, Bouharmont J (1996) Effects of salt stress on growth, mineral nutrition and proline accumulation in relation to osmotic adjustment in rice (Oryza sativa L.) cultivars differing in salinity resistance. Plant Growth Regul 19:207â218Madidi S, Baroudi B, Ameur FB (2004) Effects of salinity on germination and early growth of barley (Hordeum vulgare L.) cultivars. Int J Agric Biol 6:767â770Marchal FM, LendĂnez ML, Salazar C, Torres JA (2008) Aportaciones al conocimiento de la vegetaciĂłn gispsĂcola en el occidente de la provincia de Granada (sur de España). Lazaroa 29:95â100MĂ©dail F, Verlaque R (1997) Ecological characteristics and rarity of endemic plants from southern France and Corsica: implications for biodiversity conservation. Biol Conserv 80:269â281Meyer SE (1986) The ecology of gypsophile endemism in the Eastern Mojave desert. Ecology 67:1303â1313Moruno F, Soriano P, Oscar V, Boscaiu M, Estrelles E (2011) Opportunistic germination behaviour of Gypsophila (Caryophyllaceae) in two priority habitats from semi-arid Mediterranean steppes. Not Bot Horti Agrobo 9:18â23Mota JF, SĂĄnchez GĂłmez P, Merlo Calvente ME, CatalĂĄn RodrĂguez P, Laguna Lumbreras E, de la Cruz RM, Navarro Reyes FB, Marchal Gallardo F, BartolomĂ© Esteban C, MartĂnez Labarga JM, Sainz Ollero H, Valle Tendero F, Serra Laliga L, MartĂnez HernĂĄndez F, Garrido Becerra JA, PĂ©rez GarcĂa FJ (2009) AproximaciĂłn a la checklist de los gipsĂłfitos ibĂ©ricos. An Biol 31:71â80Mota JF, Sola AJ, JimĂ©nez-SĂĄnchez ML, PĂ©rez-GarcĂa F, Merlo ME (2004) Gypsicolous flora, conservation and restoration of quarries in the southeast of the Iberian Peninsula. Biodivers Conserv 13:1797â1808Munns R (2002) Comparative physiology of salt and water stress. Plant Cell Environ 25:239â250Palacio S, Escudero A, Montserrat-MartĂ G, Maestro M, Milla R, Albert M (2007) Plants living on gypsum: beyond the specialist model. Ann Bot 99:333â343Peinado M, MartĂnez-Parras JM (1982) Sobre la posiciĂłn fitosociolĂłgica de Gypsophila tomentosa L. Lazaroa 4:129â140Pueyo Y, Alados CL, Maestro M, Komac B (2007) Gypsophile vegetation patterns under a range of soil properties induced by topographical position. Plant Ecol 189:301â311Rasband WS (1997â2012) ImageJ. U S National Institutes of Health. http://rsb.info.nih.gov/ij/ , Bethesda, MarylandRivas-MartĂnez S (2005) Notions on dynamic-catenal phytosociology as a basis of landscape science. Plant Biosyst 139:135â144Rivas-MartĂnez S, Rivas-Saenz S (1996â2009) Worldwide bioclimatic classification system, Phytosociological Research Center, Spain. http://www.globalbioclimatics.org . Accessed 1 July 2013Rivas-MartĂnez S, FernĂĄndez-GonzĂĄlez F, Loidi J, LousĂŁ M, Penas A (2001) Syntaxonomical checklist of vascular plant communities of Spain and Portugal to association level. Itinera Geobot 14:5â341SalmerĂłn-SĂĄnchez E, MartĂnez-Nieto MI, MartĂnez-HernĂĄndez F, Garrido-Becerra JA, Mendoza-FernĂĄndez AJ, Gil de Carrasco C, Ramos-Miras JJ, Lozano R, Merlo ME, Mota JF (2014) Ecology, genetic diversity and phylogeography of the Iberian endemic plant Jurinea pinnata (Lag.) DC. (Compositae) on two special edaphic substrates: dolomite and gypsum. Plant Soil 374:233â250Saradhi P, Alia P, Arora S, Prasad KV (1995) Proline accumulates in plants exposed to UV radiation and protects them against UV induced peroxidation. Biochem Biophys Res Commun 209:1â5Sekmen AH, Turkan I, Tanyolac ZO, Ozfidan C, Dinc A (2012) Different antioxidant defense responses to salt stress during germination and vegetative stages of endemic halophyte Gypsophila oblanceolata Bark. Environ Exp Bot 77:63â76Tipirdamaz R, Gagneul D, Duhaze C, Ainouche A, Monnier C, Ozkum D, Larher F (2006) Clustering of halophytes from an inland salt marsh in Turkey according to their ability to accumulate sodium and nitrogenous osmolytes. Environ Exp Bot 57:139â153Ungar IA (1996) Effect of salinity on seed germination, growth, and ion accumulation of Atriplex patula (Chenopodiaceae). Am J Bot 83:604â607USDA-ARS (2008) Research databases. Bibliography on salt tolerance. George E. Brown, Jr. Salinity Lab. US Dep. Agric., Agric. Res. Serv. Riverside, CA. http://www.ars.usda.gov/Services/docs.htm?docid=8908USSL Staff (1954) Diagnosis and improvement of saline and alkali soils. US Department of Agriculture Handbook no. 60, 160 ppVicente O, Boscaiu M, Naranjo M, Estrelles E, BellĂ©s JM, Soriano P (2004) Responses to salt stress in the halophyte Plantago crassifolia (Plantaginaceae). J Arid Environ 58:463â48
Spatial variation in the structure and composition of the herbaceous community in a semiarid region of northeastern Brazil
Implicating genes, pleiotropy, and sexual dimorphism at blood lipid loci through multi-ancestry meta-analysis.
BACKGROUND: Genetic variants within nearly 1000 loci are known to contribute to modulation of blood lipid levels. However, the biological pathways underlying these associations are frequently unknown, limiting understanding of these findings and hindering downstream translational efforts such as drug target discovery. RESULTS: To expand our understanding of the underlying biological pathways and mechanisms controlling blood lipid levels, we leverage a large multi-ancestry meta-analysis (Nâ=â1,654,960) of blood lipids to prioritize putative causal genes for 2286 lipid associations using six gene prediction approaches. Using phenome-wide association (PheWAS) scans, we identify relationships of genetically predicted lipid levels to other diseases and conditions. We confirm known pleiotropic associations with cardiovascular phenotypes and determine novel associations, notably with cholelithiasis risk. We perform sex-stratified GWAS meta-analysis of lipid levels and show that 3-5% of autosomal lipid-associated loci demonstrate sex-biased effects. Finally, we report 21 novel lipid loci identified on the X chromosome. Many of the sex-biased autosomal and X chromosome lipid loci show pleiotropic associations with sex hormones, emphasizing the role of hormone regulation in lipid metabolism. CONCLUSIONS: Taken together, our findings provide insights into the biological mechanisms through which associated variants lead to altered lipid levels and potentially cardiovascular disease risk
A saturated map of common genetic variants associated with human height.
Common single-nucleotide polymorphisms (SNPs) are predicted to collectively explain 40-50% of phenotypic variation in human height, but identifying the specific variants and associated regions requires huge sample sizes1. Here, using data from a genome-wide association study of 5.4âmillion individuals of diverse ancestries, we show that 12,111 independent SNPs that are significantly associated with height account for nearly all of the common SNP-based heritability. These SNPs are clustered within 7,209 non-overlapping genomic segments with a mean size of around 90âkb, covering about 21% of the genome. The density of independent associations varies across the genome and the regions of increased density are enriched for biologically relevant genes. In out-of-sample estimation and prediction, the 12,111 SNPs (or all SNPs in the HapMap 3 panel2) account for 40% (45%) of phenotypic variance in populations of European ancestry but only around 10-20% (14-24%) in populations of other ancestries. Effect sizes, associated regions and gene prioritization are similar across ancestries, indicating that reduced prediction accuracy is likely to be explained by linkage disequilibrium and differences in allele frequency within associated regions. Finally, we show that the relevant biological pathways are detectable with smaller sample sizes than are needed to implicate causal genes and variants. Overall, this study provides a comprehensive map of specific genomic regions that contain the vast majority of common height-associated variants. Although this map is saturated for populations of European ancestry, further research is needed to achieve equivalent saturation in other ancestries
Initial constraints in seedling dynamics of Juniperus macrocarpa Sm.
The micro-forests dominated by Juniperus
macrocarpa inhabiting coastal dunes in the Mediterranean
Basin are European priority habitats. Their
conservation is hindered by both anthropic and natural
causes, although the regeneration problems for J.
macrocarpa are still poorly understood. In this study,
several factors influencing emergence and mortality of
J. macrocarpa seedlings were investigated. For this
purpose, permanent plots were placed in coastal dunes
of Sardinia (Italy) and periodically monitored to
record seedling parameters (emergence, survival and
growth), as well as several biotic and abiotic variables
(solar radiation, tree cover, herbaceous plus scrub
cover, number of female cones on the soil and number
of herbivory traces). Linear mixed models were fitted
to test the effects of several types of explanatory
variables on seedling dynamics. A total of 536
seedlings were marked, most of which emerged in
winter. The microhabitat was the most important
factor in models explaining emergence, with the
number of emerged seedlings decreasing with solar
radiation. Survival was very low, reaching the highest
mortality percentage in the first summer. Herbivory
and location in open interspaces had significant
negative effects on seedling survival. In conclusion,
the recruitment of J. macrocarpa is highly limited in
the initial life cycle phases, with microhabitat and
herbivory constraints influencing seedling dynamics.
The identification of critical stages in the recruitment
process of J. macrocarpa, together with factors
influencing them, suggests some implication for
management as well the hypotheses for future studies
about conservation and recovery of the J. macrocarpa
habitat
Inter-and intraspecific morphometric variability in Juniperus L. seeds (Cupressaceae)
In this study, a statistical classifier for Mediterranean taxa of Juniperus, based on 98 seed morphometric parameters, was tested at interspecific, specific and intraspecific levels. Ripe cones of 10 taxa were collected in different regions of the Mediterranean Basin to compare and discuss their taxonomic treatments according to two different sources. High percentages of correct identification were reached for both taxonomic treatments at the specific and intraspecific level and from the comparison among taxa of the J. oxycedrus, J. communis and J. phoenicea complexes. Moreover, ripe cones of J. macrocarpa were collected from four Sardinian populations, in two seasons, and from plant and soil, in order to analyse inter-population, seasonal and source variability in seed morphology. This statistical classifier discriminated J. macrocarpa seeds collected in spring more accurately than those collected in autumn, but it failed to distinguish between the seeds collected from plants and soil, or between those collected from different populations of the same geographic region
Hotspots within hotspots: Endemic plant richness, environmental drivers, and implications for conservation
Detecting smaller hotspots within larger hotspots could be an essential tool to focus conservation efforts.
In this study, we identified hotspots at two scales of analysis within the Mediterranean overall hotspot.
Particularly, based on the distribution of endemic-vascular-plant richness (EVPR), we identified microhotspots,
among the richest floristic territories of the Sardinian and Baetic regions, and nano-hotspots,
among the richest 1-km2 grid cells of Sierra Nevada and Gennargentu massifs, located within these
regions. In addition, we explored environmental drivers of EVPR, performing both simple- and multiple-
regression models. Our results showed that even in areas previously defined as hotspots, the endemic-
plant richness was not uniformly distributed, but rather depended largely on environmental
conditions. Relationships between environmental drivers and EVPR have been poorly studied in the Mediterranean
context, where we found patterns consistent among scales and regions. Specifically, EVPR was
positively linked to altitude and precipitation, particularly in the driest period. Hence, the different levels
of hotspots nested in hotspots were organized in a hierarchy. This downscaling approach may help to
focus conservation efforts within a given hotspot, e.g. the identification of narrow hotspots could be useful
to find gaps in the protected-area networks. Specifically, the identified nano-hotspots are certainly
priority sites for plant conservation, since the whole of the nano-hotspots in each region represented less
than 1% of the surface area but contained more than 19% of the regional EVPR. Moreover, an examination
of both where hotspots are and under what environmental conditions they appear, would enable the
detection of specific threat
Hotspots within hotspots: Endemic plant richness, environmental drivers, and implications for conservation
Detecting smaller hotspots within larger hotspots could be an essential tool to focus conservation efforts.
In this study, we identified hotspots at two scales of analysis within the Mediterranean overall hotspot.
Particularly, based on the distribution of endemic-vascular-plant richness (EVPR), we identified microhotspots,
among the richest floristic territories of the Sardinian and Baetic regions, and nano-hotspots,
among the richest 1-km2 grid cells of Sierra Nevada and Gennargentu massifs, located within these
regions. In addition, we explored environmental drivers of EVPR, performing both simple- and multiple-
regression models. Our results showed that even in areas previously defined as hotspots, the endemic-
plant richness was not uniformly distributed, but rather depended largely on environmental
conditions. Relationships between environmental drivers and EVPR have been poorly studied in the Mediterranean
context, where we found patterns consistent among scales and regions. Specifically, EVPR was
positively linked to altitude and precipitation, particularly in the driest period. Hence, the different levels
of hotspots nested in hotspots were organized in a hierarchy. This downscaling approach may help to
focus conservation efforts within a given hotspot, e.g. the identification of narrow hotspots could be useful
to find gaps in the protected-area networks. Specifically, the identified nano-hotspots are certainly
priority sites for plant conservation, since the whole of the nano-hotspots in each region represented less
than 1% of the surface area but contained more than 19% of the regional EVPR. Moreover, an examination
of both where hotspots are and under what environmental conditions they appear, would enable the
detection of specific threat