Cardiac sarcoplasmic reticulum characteristics in hypertrophic hearts from spontaneously hypertensive rats

Sarcoplasmic reticulum (SR) from left ventricles of rats that developed spontaneous hypertension was studied in vitro. Similar increases of left ventricular mass were found when grouping the animals into mild and severe hypertensives (average systolic arterial pressure of 168±4 and 202±6 mm Hg, respectively). The amount of SR protein (mg/g of left ventricle) was higher when obtained from hypertrophic ventricles of both hypertensive groups than from ventricles of the control group. The result agreed with the enhanced Ca2+ uptake exhibited by left ventricular homogenates of the hypertensive groups. Consequently, Ca2+ uptake in SR microsomes isolated per gram of left ventricle (nmol Ca2+/g muscle) was 51.62±10.06 and 64.99±12.84 in mildly and severely hypertensive groups vs. 17.37±5.79 in the control group (P<0.05). The SR microsomes obtained from ventricles of hypertensive rats showed an enhanced Ca2+ activated ATPase activity that was not accompanied by increased Ca2+ uptake at saturating calcium concentrations, but by increased affinity for calcium (K'app of 1.09±0.28 and 2.67±0.16 μM in SR microsomes of hypertrophic and control ventricles respectively; P<0.05). The rats of calcium loss, measured in SR vesicles passively loaded with45Ca, were similar when assayed in SR obtained from ventricles of both hypertensive and normal rats. These results enable us to suggest that in hearts of rats presenting spontaneous hypertension, the function of the SR system could account for a normal handling of cytosolic calcium. They might support the absence of mechanical alterations described in hearts of young rats of the SHR strain.Centro de Investigaciones Cardiovasculare

Azimuthal clumping instabilities in a ZZ-pinch wire array

A simple model is constructed to evaluate the temporal evolution of azimuthal clumping instabilities in a cylindrical array of current-carrying wires. An analytic scaling law is derived, which shows that randomly seeded perturbations evolve at the rate of the fastest unstable mode, almost from the start. This instability is entirely analogous to the Jeans instability in a self-gravitating disk, where the mutual attraction of gravity is replaced by the mutual attraction among the current-carrying wires.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/87765/2/052701_1.pd

Radio frequency plasma processing effects on the emission characteristics of a MeV electron beam cathode

Experiments have proven that surface contaminants on the cathode of an electron beam diode influence electron emission current and impedance collapse. This letter reports on an investigation to reduce parasitic cathode current loss and to increase high voltage hold off capabilities by reactive sputter cleaning of contaminants. Experiments have characterized effective radio frequency (rf) plasma processing protocols for high voltage anode–cathode (A–K) gaps using a two-stage argon/oxygen and argon rf plasma discharge. Time-resolved optical emission spectroscopy measures contaminant (hydrogen) and bulk cathode (aluminum) plasma emission versus transported axial electron beam current turn on. Experiments were performed at accelerator parameters: V = −0.7V=−0.7 to −1.1 MV,−1.1MV, I(diode)=3–30 kA,I(diode)=3–30kA, and pulse length=0.4–1.0 μs.length=0.4–1.0μs. Experiments using a two-stage low power (100 W) argon/oxygen rf discharge followed by a higher power (200 W) pure argon rf discharge yielded an increase in cathode turn-on voltage required for axial current emission from 662±174 kV662±174kV to 981±97 kV.981±97kV. The turn-on time of axial current was increased from 100±22100±22 to 175±42 ns.175±42ns. © 1999 American Institute of Physics.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/69353/2/APPLAB-75-1-31-1.pd

Microwave apparatus for gravitational waves observation

In this report the theoretical and experimental activities for the development of superconducting microwave cavities for the detection of gravitational waves are presented.Comment: 42 pages, 28 figure

Caterpillar structures in single-wire Z-pinch experiments

A series of experiments have been performed on single-wire Z pinches (1–2 kA, 20 kV, pulse length 500 ns; Al, Ag, W, or Cu wire of diameter 7.5–50 μm, length 2.5 cm). Excimer laser absorption photographs show expansion of metallic plasmas on a time scale of order 100 ns. The edge of this plasma plume begins to develop structures resembling a caterpillar only after the current pulse reaches its peak value. The growth of these caterpillar structures is shown to be consistent with the Rayleigh–Taylor instability of the decelerating plasma plume front at the later stage of the current pulse. © 2003 American Institute of Physics.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/71205/2/APPLAB-83-24-4915-1.pd

White matter connectome edge density in children with Autism Spectrum Disorders: potential imaging biomarkers using machine learning models

Prior neuroimaging studies have reported white matter network underconnectivity as a potential mechanism for Autism Spectrum Disorder (ASD). In this study, we examined the structural connectome of children with ASD using Edge Density Imaging (EDI); and then applied machine leaning algorithms to identify children with ASD based on tract-based connectivity metrics. Boys aged 8 to 12 years were included: 14 with ASD and 33 typically developing children (TDC). The Edge Density (ED) maps were computed from probabilistic streamline tractography applied to high angular resolution diffusion imaging (HARDI). Tract-Based Spatial Statistics (TBSS) was used for voxel-wise comparison and coregistration of ED maps in addition to conventional DTI metrics of Fractional Anisotropy (FA), Mean Diffusivity (MD), and Radial Diffusivity (RD). Tract-based average DTI/connectome metrics were calculated and used as input for different machine learning models: naïve Bayes, random forest, support vector machines (SVM), neural networks. For these models, cross-validation was performed with stratified random sampling (×1000 permutations). The average accuracy among validation samples was calculated. In voxel-wise analysis, the body and splenium of corpus callosum, bilateral superior and posterior corona radiata, and left superior longitudinal fasciculus showed significantly lower ED in children with ASD; whereas, we could not find significant difference in FA, MD, and RD maps between the two study groups. Overall, machine-learning models using tract-based ED metrics had better performance in identification of children with ASD compared to those using FA, MD, and RD. The EDI-based random forest models had greater average accuracy (75.3%), specificity (97.0%), and positive predictive value (81.5%), whereas EDI-based polynomial SVM had greater sensitivity (51.4%), and negative predictive values (77.7%). In conclusion, we found reduced density of connectome edges in the posterior white matter tracts of children with ASD; and demonstrated the feasibility of connectome-based machine-learning algorithms in identification of children with ASD

NEMO: A Project for a km3^3 Underwater Detector for Astrophysical Neutrinos in the Mediterranean Sea

The status of the project is described: the activity on long term characterization of water optical and oceanographic parameters at the Capo Passero site candidate for the Mediterranean km$^3$ neutrino telescope; the feasibility study; the physics performances and underwater technology for the km$^3$; the activity on NEMO Phase 1, a technological demonstrator that has been deployed at 2000 m depth 25 km offshore Catania; the realization of an underwater infrastructure at 3500 m depth at the candidate site (NEMO Phase 2).Comment: Proceeding of ISCRA 2006, Erice 20-27 June 200

Radiation Science Using Z-Pinch X-Rays

Present-day Z-pinch experiments generate 200 TW peak power, 5–10 ns duration x-ray bursts that provide new possibilities to advance radiation science. The experiments support both the underlying atomic and plasma physics, as well as inertial confinement fusion and astrophysics applications. A typical configuration consists of a sample located 1–10 cm away from the pinch, where it is heated to 10–100 eV temperatures by the pinch radiation. The spectrally-resolved sample-plasma absorption is measured by aiming x-ray spectrographs through the sample at the pinch. The pinch plasma thus both heats the sample and serves as a backlighter. Opacitymeasurements with this source are promising because of the large sample size, the relatively long radiation duration, and the possibility to measureopacities at temperatures above 100 eV. Initial opacity experiments are under way with CH-tamped NaBr foil samples. The Na serves as a thermometer and absorption spectra are recorded to determine the opacity of Br with a partially-filled M-shell. The large sample size and brightness of the Z pinch as a backlighter are also exploited in a novel method measuring re-emission from radiation-heated gold plasmas. The method uses a CH-tamped layered foil with Al+MgF2 facing the radiationsource. A gold backing layer that covers a portion of the foil absorbs radiation from the source and provides re-emission that further heats the Al+MgF2. The Al and Mg heating is measured using space-resolved Kα absorption spectroscopy and the difference between the two regions enables a determination of the gold re-emission. Measurements are also performed at lower densities where photoionization is expected to dominate over collisions. Absorption spectra have been obtained for both Ne-like Fe and He-like Ne, confirming production of the relevant charge states needed to benchmark atomic kinetics models. Refinement of the methods described here is in progress to address multiple issues for radiation science

Imaging Primary Mouse Sarcomas After Radiation Therapy Using Cathepsin-Activatable Fluorescent Imaging Agents

Purpose: Cathepsin-activated fluorescent probes can detect tumors in mice and in canine patients. We previously showed that these probes can detect microscopic residual sarcoma in the tumor bed of mice during gross total resection. Many patients with soft tissue sarcoma (STS) and other tumors undergo radiation therapy (RT) before surgery. This study assesses the effect of RT on the ability of cathepsin-activated probes to differentiate between normal and cancerous tissue. Methods and Materials: A genetically engineered mouse model of STS was used to generate primary hind limb sarcomas that were treated with hypofractionated RT. Mice were injected intravenously with cathepsin-activated fluorescent probes, and various tissues, including the tumor, were imaged using a hand-held imaging device. Resected tumor and normal muscle samples were harvested to assess cathepsin expression by Western blot. Uptake of activated probe was analyzed by flow cytometry and confocal microscopy. Parallel in vitro studies using mouse sarcoma cells were performed. Results: RT of primary STS in mice and mouse sarcoma cell lines caused no change in probe activation or cathepsin protease expression. Increasing radiation dose resulted in an upward trend in probe activation. Flow cytometry and immunofluorescence showed that a substantial proportion of probe-labeled cells were CD11b-positive tumor-associated immune cells. Conclusions: In this primary murine model of STS, RT did not affect the ability of cathepsin-activated probes to differentiate between tumor and normal muscle. Cathepsin-activated probes labeled tumor cells and tumor-associated macrophages. Our results suggest that it would be feasible to include patients who have received preoperative RT in clinical studies evaluating cathepsin-activated imaging probes.Damon Runyon Cancer Research Foundation (Damon Runyon-Rachleff Innovation Award