DOH: A Content Delivery Peer-to-Peer Network

Many SMEs and non-pro¯t organizations su®er when their Web servers become unavailable due to °ash crowd e®ects when their web site becomes popular. One of the solutions to the °ash-crowd problem is to place the web site on a scalable CDN (Content Delivery Network) that replicates the content and distributes the load in order to improve its response time. In this paper, we present our approach to building a scalable Web Hosting environment as a CDN on top of a structured peer-to-peer system of collaborative web-servers integrated to share the load and to improve the overall system performance, scalability, availability and robustness. Unlike clusterbased solutions, it can run on heterogeneous hardware, over geographically dispersed areas. To validate and evaluate our approach, we have developed a system prototype called DOH (DKS Organized Hosting) that is a CDN implemented on top of the DKS (Distributed K-nary Search) structured P2P system with DHT (Distributed Hash table) functionality [9]. The prototype is implemented in Java, using the DKS middleware, the Jetty web-server, and a modi¯ed JavaFTP server. The proposed design of CDN has been evaluated by simulation and by evaluation experiments on the prototype

Plant immunity and beyond: Signals from proteins & peptides

Plants are the primary and most important source of food for human consumption, besides their ecological importance, since they define the diverse ecosystems worldwide. Because of their position in the ecological chain as primary supplier of biomass and food for other organisms, plants are also fundamental for animals, fungi and microorganisms, and some of them are also beneficial to other plants. Humans rely mostly on plant products for food, and many plants provide important non-food products, including wood, textiles, medicines, cosmetics, soaps, rubber, plastics, paints and other industrial chemicals. Moreover, plants are also fundamental for animal feeding, including not only mammals (e.g. cattle, sheep and goats), but also poultry and aquaculture (e.g., fish and shrimp farming)

Runtime Distributions and Criteria for Restarts

Randomized algorithms sometimes employ a restart strategy. After a certain number of steps, the current computation is aborted and restarted with a new, independent random seed. In some cases, this results in an improved overall expected runtime. This work introduces properties of the underlying runtime distribution which determine whether restarts are advantageous. The most commonly used probability distributions admit the use of a scale and a location parameter. Location parameters shift the density function to the right, while scale parameters affect the spread of the distribution. It is shown that for all distributions scale parameters do not influence the usefulness of restarts and that location parameters only have a limited influence. This result simplifies the analysis of the usefulness of restarts. The most important runtime probability distributions are the log-normal, the Weibull, and the Pareto distribution. In this work, these distributions are analyzed for the usefulness of restarts. Secondly, a condition for the optimal restart time (if it exists) is provided. The log-normal, the Weibull, and the generalized Pareto distribution are analyzed in this respect. Moreover, it is shown that the optimal restart time is also not influenced by scale parameters and that the influence of location parameters is only linear

HIV-1 mother-to-child transmission in Brazil (1994\u20132016): a time series modeling

HIV-1 mother-to-child transmission (HIV-1 MTCT), is an important cause of children mortality worldwide. Brazil has been traditionally praised by its HIV/Aids program, which provides free-of-charge care for people living with HIV-1. Using public epidemiology and demographic databases, we aimed at modeling HIV-1 MTCT prevalence in Brazil through the years (1994\u20132016) and elaborate a statistical model for forecasting, contributing to HIV-1 epidemiologic surveillance and healthcare decision-making. We downloaded sets of live births and mothers\u2019 data alongside HIV-1 cases notification in children one year old or less. Through time series modeling, we estimated prevalence along the years in Brazil, and observed a remarkable decrease of HIV-1 MTCT between 1994 (10 cases per 100,000 live births) and 2016 (five cases per 100,000 live births), a reduction of 50%. Using our model, we elaborated a prognosis for each Brazilian state to help HIV-1 surveillance decision making, indicating which states are in theory in risk of experiencing a rise in HIV-1 MTCT prevalence. Ten states had good (37%), nine had mild (33%), and eight had poor prognostics (30%). Stratifying the prognostics by Brazilian region, we observed that the Northeast region had more states with poor prognosis, followed by North and Midwest, Southeast and South with one state of poor prognosis each. Brazil undoubtedly advanced in the fight against HIV-1 MTCT in the past two decades. We hope our model will help indicating where HIV-1 MTCT prevalence may rise in the future and support government decision makers regarding HIV-1 surveillance and prevention

Inflammasome activation by NLRP1 and NLRC4 in patients with coronary stenosis

Objective and design: We performed an experimental, analytical and prospective study to evaluate the systemic activation of inflammasome in atherosclerosis\u2019 patients, in order to shed light into responsible mechanisms for plaque formation. Subjects: We included sixty individuals distributed into 3 groups: 2 groups based on the report from the angiography (severe lesions - SL and primary lesions - PL) and 1 group enclosing healthy individuals (HC). Methods: The expression assays of inflammasome genes NLRP1, NLRC4, CASP-1 and IL-1\u3b2 were performed using Real Time qPCR, with specific Taqman Assays. IL-1\u3b2 serum levels were analysed by commercial kit. Were applied the Shapiro-Wilk and Student's T-test as statistical tests. Statistical significance was set to p 64 0.05. Results: Upregulation of NLRP1 (+3.47 FC, p = 0.0001), NLRC4 (+7.06 FC, p = 6.792 7 10 1209) and IL-1\u3b2 (+2.43 FC, p = 0.005) was observed in all atherosclerosis patients when compared to HC. According to stenosis severity, patients with primary lesions showed upregulation of inflammasome genes NLRP1 (+2.87 FC, p = 0.0008), NLRC4 (+6.34 FC, p = 4.134 7 10-07) and IL-1\u3b2 (+3.39 FC, p = 0.0012) with respect to the HC group. No statistical difference was found in IL-1\u3b2 serum levels according the assessed groups. Conclusions: Inflammasome activation in atherosclerosis's patients can be systemic altered and may be triggered by NLRP1 and NLRC4 receptors. IL-1\u3b2 gene expression was identified in our study as an important systemic detectable marker of plaque severity

Optimization of Anti-SARS-CoV-2 Treatments Based on Curcumin, Used Alone or Employed as a Photosensitizer.

Curcumin, the bioactive compound of the spice Curcuma longa, has already been reported as a potential COVID-19 adjuvant treatment due to its immunomodulatory and anti-inflammatory properties. In this study, SARS-CoV-2 was challenged with curcumin; moreover, curcumin was also coupled with laser light at 445 nm in a photodynamic therapy approach. Curcumin at a concentration of 10 μM, delivered to the virus prior to inoculation on cell culture, inhibited SARS-CoV-2 replication (reduction >99%) in Vero E6 cells, possibly due to disruption of the virion structure, as observed using the RNase protection assay. However, curcumin was not effective as a prophylactic treatment on already-infected Vero E6 cells. Notably, when curcumin was employed as a photosensitizer and blue laser light at 445 nm was delivered to a mix of curcumin/virus prior to the inoculation on the cells, virus inactivation was observed (>99%) using doses of curcumin that were not antiviral by themselves. Photodynamic therapy employing crude curcumin can be suggested as an antiviral option against SARS-CoV-2 infection

Unusual onset of a case of chronic recurrent multifocal osteomyelitis

Background: Chronic recurrent multifocal osteomyelitis (CRMO) is a rare condition that commonly affects the clavicle and pelvis. Case presentation: We report here a case a 12 years old girl with CRMO arising with recurrent episodes of left supraorbital headache, followed by the appearance of a periorbital dyschromia. Magnetic resonance imaging (MRI) of the skull and orbits revealed an important subacute inflammatory process. Few months after, the child presented a painful swelling of the left clavicle; the histological examination of the related biopsy allowed to establish the diagnosis of CRMO. Conclusion: CRMO presenting as acute headache involving neurocranium is rare; to our knowledge this is the first recognized case in the world literature. This pathological condition is frequently misdiagnosed as infection or neoplasm and needs a deep investigation for the differential diagnosis. The physical, laboratoristic and instrumental diagnostic investigations of the patient and the treatment employed are described in detail

Association of TNF-\u3b1, CTLA4, and PTPN22 polymorphisms with type 1 diabetes and other autoimmune diseases in Brazil

Type 1 diabetes mellitus (T1D) is a complex disorder characterized by an autoimmune response against human pancreatic beta-cells. Patients with T1D can also develop a response toward one or more other factors, such as in autoimmune thyroiditis (AITD) and celiac disease (CD). In the presence of T1D + AITD, the patient is diagnosed with autoimmune polyglandular syndrome type III (APSIII); patients with APSIII may also present with CD. These diseases have a strong genetic component and share many susceptibility genes, suggesting potentially overlapping pathogenic pathways. Polymorphisms in the TNF-\u3b1(rs1800629), CTLA-4 (rs231775), and PTPN22 (rs2476601) genes have been previous associated with T1D; however, there is no consensus regarding their role in T1D and scarce literature focusing on AIDT and/or CD. Thus, we analyzed these genetic variants in 205 Northeast Brazilian patients with T1D and with/without AITD and/or CD, and in 308 healthy controls. The PTPN22 gene variants were associated with T1D susceptibility and APSIII [odds ratio (OR) = 2.57 and 2.77, respectively]. CTLA4 rs231775 and TNF-\u3b1rs1800629 were not associated with T1D onset in the Brazilian population. However, when comparing APSIII individuals in the T1D only group, we observed an association of the TNF-\u3b1SNP in the allelic (P = 0.0442; OR = 0.44) and dominant models (P = 0.0387; OR = 0.40). This study reinforces the importance of CTLA-4 and other variants in unraveling the pathogenic mechanisms of T1D in different populations and in understanding their relationships with the development of other T1D-related autoimmune diseases

Photobiomodulation therapy is able to decrease IL1B gene expression in an in vitro cellular model of hidradenitis suppurativa

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