138 research outputs found
Optimization conditions of UV-C radiation combined with ultrasound-assisted extraction of cherry tomato (Lycopersicon esculentum) lycopene extract
The aim of this work was to study the effect of UV-C radiation on ultrasound assisted extraction
(UAE) of cherry tomato bioactive compounds. Cherry tomatoes were exposed to two UV-C radiation
doses (0.5 and 1.0 J cmâ2
) and stored at 20 ± 0.5 oC for 7 days. Next, they were lyophilized, and
the bioactive compounds were extracted by UAE at 20 KHz. To evaluate the effectiveness of the
extraction process of the bioactive compounds, a CCRD (central composite rotational design) was
used together with RSM (response surface methodology), for extraction times from 4 to 12 minutes
and concentrations (g of lyophilized product / L of ethanol) of 1:10, 1:20 and 1:30. The extracts
obtained from the irradiated tomatoes presented 5.8 times more lycopene content than the controls
and higher antioxidant activity was obtained for 4 and 8 min, in the concentrations 1:10 and 1:20 (m
vâ1). Through numerical model optimization, optimal extraction conditions were obtained. The results
demonstrated that by previously irradiating tomatoes with UV-C light, the UAE yielded considerably
higher amounts of lycopene and other bioactives.CNPq (National Council of Technological and Scientific
Development, Brazil), Erasmus Mundus action 2; Fellow
Mundus Project; Department of Chemical Engineering and Food Engineering
(UFSC - Brazil) and the Department of Food Engineering (UAlg - Portugal) .info:eu-repo/semantics/publishedVersio
The uncertainty principle and classical amplitudes
We study the variance in the measurement of observables during scattering events, as computed using amplitudes. The classical regime, characterised by negligible uncertainty, emerges as a consequence of an infinite set of relationships among multileg, multiloop amplitudes in a momentum-transfer expansion. We discuss two non-trivial examples in detail: the six-point tree and the five-point one-loop amplitudes in scalar QED. We interpret these relationships in terms of a coherent exponentiation of radiative effects in the classical limit which generalises the eikonal formula, and show how to recover the impulse, including radiation reaction, from this generalised eikonal. Finally, we incorporate the physics of spin into our framework
Costruire la memoria: uso e abuso della storia fra tarda repubblica e primo principato.
Il volume, che raccoglie gli atti del Convegno Interazionale "Costruire la memoria: uso e abuso della storia fra tarda repubblica e primo principato" svoltosi a Venezia il 14 e il 15 gennaio 2016, indaga, attraverso casi individuati dai contributori, come la ricostruzione della storia tardo repubblicana e della prima etĂ imperiale nelle fonti sia oggetto di manipolazioni diverse per tipologia, finalitĂ , esiti; origini tradizioni contrastanti su molti momenti del passato e si traduca in un importante strumento della politica. Il Convegno, di cui il volume pubblica i contributi, ha rappresentato il terzo appuntamento congressuale (dopo le esperienze del 2010 e del 2013) organizzato dal gruppo di ricerca "Fra repubblica e principato", diretto da R. Cristofoli, A. Galimberti e F. Rohr Vio, che si propone di indagare l storia del I secolo a.C.-I secolo d.C. mediante il coinvolgimento di specialisti che consentano l'impostazione di studi interdisciplinari di respiro internazionale
Comparative study of the production of cellulose nanofibers from agro-industrial waste streams of Salicornia ramosissima by acid and enzymatic treatment
The study of the suitability of two isolation processes to produce cellulose nanofibers (CNFs) from Salicornia ramosissima waste, with potential applicability as a reinforcing agent of polymeric composites was carried out. To separate the cellulose fibrils from the cell wall and obtain CNFs an alkaline treatment was applied followed by a bleaching treat-ment and, the insoluble residue was next hydrolyzed by either an acid treatment (AT) or an enzyme treatment (ET). SEM and TEM images indicated fiber exposure caused by both treatments. The diameter, length, aspect ratio, and polydispersity index, were measured for both CNFs. CNF (ET) showed high zeta potential values suggesting that ET produces more electrically stable and thinner nanofibers. The FTIR spectra revealed that both treatments effectively removed the amorphous components allowing the CNFs isolation, and XRD patterns evidenced the increase in the degree of crystallinity of both CNFs. Nonetheless, CNF(AT) presented a lower mechanical resistance due to its smaller particle size, compared to the CNF(ET). In summary, the (ET) could successfully isolate CNFs from the Salicornia waste, encouraging the use of this treatment, once when compared to (AT), it does not generate toxic residues, presents mild thermal conditions, and produces CNFs with higher-value applications.info:eu-repo/semantics/publishedVersio
Towards a Long-Read Sequencing Approach for the Molecular Diagnosis of RPGRORF15 Genetic Variants
Sequencing of the low-complexity ORF15 exon of RPGR, a gene correlated with retinitis pigmentosa and cone dystrophy, is difficult to achieve with NGS and Sanger sequencing. False results could lead to the inaccurate annotation of genetic variants in dbSNP and ClinVar databases, tools on which HGMD and Ensembl rely, finally resulting in incorrect genetic variants interpretation. This paper aims to propose PacBio sequencing as a feasible method to correctly detect genetic variants in low-complexity regions, such as the ORF15 exon of RPGR, and interpret their pathogenicity by structural studies. Biological samples from 75 patients affected by retinitis pigmentosa or cone dystrophy were analyzed with NGS and repeated with PacBio. The results showed that NGS has a low coverage of the ORF15 region, while PacBio was able to sequence the region of interest and detect eight genetic variants, of which four are likely pathogenic. Furthermore, molecular modeling and dynamics of the RPGR Glu-Gly repeats binding to TTLL5 allowed for the structural evaluation of the variants, providing a way to predict their pathogenicity. Therefore, we propose PacBio sequencing as a standard procedure in diagnostic research for sequencing low-complexity regions such as RPGRORF15, aiding in the correct annotation of genetic variants in online databases
CREBBP mutations in individuals without Rubinstein-Taybi syndrome phenotype
Item does not contain fulltextMutations in CREBBP cause Rubinstein-Taybi syndrome. By using exome sequencing, and by using Sanger in one patient, CREBBP mutations were detected in 11 patients who did not, or only in a very limited manner, resemble Rubinstein-Taybi syndrome. The combined facial signs typical for Rubinstein-Taybi syndrome were absent, none had broad thumbs, and three had only somewhat broad halluces. All had apparent developmental delay (being the reason for molecular analysis); five had short stature and seven had microcephaly. The facial characteristics were variable; main characteristics were short palpebral fissures, telecanthi, depressed nasal ridge, short nose, anteverted nares, short columella, and long philtrum. Six patients had autistic behavior, and two had self-injurious behavior. Other symptoms were recurrent upper airway infections (n = 5), feeding problems (n = 7) and impaired hearing (n = 7). Major malformations occurred infrequently. All patients had a de novo missense mutation in the last part of exon 30 or beginning of exon 31 of CREBBP, between base pairs 5,128 and 5,614 (codons 1,710 and 1,872). No missense or truncating mutations in this region have been described to be associated with the classical Rubinstein-Taybi syndrome phenotype. No functional studies have (yet) been performed, but we hypothesize that the mutations disturb protein-protein interactions by altering zinc finger function. We conclude that patients with missense mutations in this specific CREBBP region show a phenotype that differs substantially from that in patients with Rubinstein-Taybi syndrome, and may prove to constitute one (or more) separate entities. (c) 2016 Wiley Periodicals, Inc
The Weyl double copy from twistor space
The Weyl double copy is a procedure for relating exact solutions in biadjoint scalar, gauge and gravity theories, and relates fields in spacetime directly. Where this procedure comes from, and how general it is, have until recently remained mysterious. In this paper, we show how the current form and scope of the Weyl double copy can be derived from a certain procedure in twistor space. The new formalism shows that the Weyl double copy is more general than previously thought, applying in particular to gravity solutions with arbitrary Petrov types. We comment on how to obtain anti-self-dual as well as self-dual fields, and clarify some conceptual issues in the twistor approach
COMPARAĂĂO DO EFEITO DO TREINAMENTO PROPRIOCEPTIVO NO TORNOZELO DE NĂO ATLETAS E JOGADORES DE VOLEIBOL
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