Seasonal patterns of metals and nutrients in Phragmites australis (Cav.) Trin. ex Steudel in a constructed wetland in the west of Ireland

© 2017 Elsevier B.V. An understanding of the seasonal variation in the standing stock of metals and nutrients in emergent vegetation of constructed wetlands (CWs), as well as the amounts present in aboveground (AG) and belowground (BG) biomass, is crucial to their design and management. Given that biomass harvesting is a labour and time consuming operation, a paucity of information currently exists on accumulation and standing stocks in biomass in CWs, in particular in North Western European countries. To address this knowledge gap, this paper examined the seasonal variations of metals and nutrients in Phragmites australis (Cav.) Trin. ex Steudel in a CW treating municipal wastewater, with a view to identifying an optimal time for biomass harvesting of the AG vegetation. Although the AG biomass was greatest in August (1636 ± 507 g m −2 ), the maximum concentrations and accumulations of metals and nutrients occurred at different times throughout the duration of the study (April to November). Furthermore, with the exception of zinc and nitrogen, metals and nutrients measured in BG biomass ranged from 66% (phosphorus) to greater than 80% (nickel and chromium) of the AG biomass. This indicates that analysis of only the emergent shoots may significantly underestimate the metal and nutrient uptake and capacity of the plant. In order to effectively target the bulk of metals and nutrients, an AG harvest in late August or September is suggested

Diagnostic definitions and figures of male and female Tetanocera punctifrons and T. latifibula, new records of T. punctifrons in Ireland, and notes on biology (Diptera: Sciomyzidae).

The presence of Tetanocera punctifrons Rondani, 1868 in Ireland is reconfirmed. The species is distinguished from the very similar T. latifibula Frey, 1924. The female abdomens of both species are described for the first time. The distributions of both species are summarised. The history of type examinations and of taxonomists’ conceptions of the two species is tracked, especially the relative reliability of published figures of diagnostic features. We emphasise the need for such analyses of rare and closely related species, even if apparently disjunct in distribution. Habitats of T. punctifrons and T. latifibula are described, and the biology and morphology of the immature stages are compared

Sciomyzidae (Diptera) Assemblages in Constructed and Natural Wetlands: Implications for Constructed Wetland Design

Wetlands constructed primarily for the treatment of wastewaters have been shown to have a role in enhancing biodiversity. However, while most biodiversity studies of constructed wetlands focus on the larger, more iconic animal groups, there is a paucity of information on the aerial phases of wetland invertebrate species associated with constructed wetlands. This study compares Sciomyzidae (Diptera) assemblages, established indicators of wetland dipteran communities, in Irish constructed and natural wetlands, in addition to determining the impacts of water quality and surrounding habitats on Sciomyzidae community structure. Natural wetlands had significantly greater species richness, abundances and diversity (measured as Shannon’s entropy) of sciomyzid flies than constructed wetlands. Nevertheless, although concentrations of nitrogen and phosphorus in waters were significantly greater in constructed wetlands, seven of the eight constructed wetlands examined hosted species of Sciomyzidae listed as scarce or threatened in Britain. In addition, sciomyzid species richness increased as areas of semi-natural habitat immediately surrounding constructed and natural wetlands increased. Composition of Sciomyzidae assembalges in both natural and constructed wetlands were analysed. The results of this study demonstrate that constructed wetlands can be important contributors to biodiversity particularly in the context of current losses of natural wetlands worldwide. The importance of habitats immediately surrounding constructed wetlands also highlights the need for relatively simple design recommendations (e.g. wet grassland creation or judicious planting of wetland trees) that could enhance the biodiversity of existing and future constructed wetlands

Suicide attempts in clinical trials with paroxetine randomised against placebo

BACKGROUND: Inclusion of unpublished data on the effects of antidepressants on children has suggested unfavourable risk-benefit profiles for some of the drugs. Recent meta-analyses of studies on adults have indicated similar effects. We obtained unpublished data for paroxetine that have so far not been included in these analyses. METHODS: The documentation for drug registration contained 16 studies in which paroxetine had been randomised against placebo. We registered the number of suicides, suicide attempts and ideation. We corrected for duration of medication and placebo treatment and used a standard Bayesian statistical approach with varying priors. RESULTS: There were 7 suicide attempts in patients on the drug and 1 in a patient on placebo. We found that the probability of increased intensity of suicide attempts per year in adults taking paroxetine was 0.90 with a "pessimistic" prior, and somewhat less with two more neutral priors. CONCLUSION: Our findings support the results of recent meta-analyses. Patients and doctors should be warned that the increased suicidal activity observed in children and adolescents taking certain antidepressant drugs may also be present in adults

Habitat suitability assessment of constructed wetlands for the smooth newt (Lissotriton vulgaris [Linnaeus, 1758]): A comparison with natural wetlands

Given the current decline of natural wetlands worldwide and the consequent negative impacts on amphibians, wetlands constructed for the treatment of wastewaters have the potential to play a role in the protection of these animals. However, there is a paucity of information regarding the value of constructed wetlands (CWs) to amphibians, particularly relating to the terrestrial phase of their life-cycle. This study compares the terrestrial habitats of natural wetlands (NWs) and CWs as refuges for the smooth newt (Lissotriton vulgaris, [L., 1758]) with the aim of developing recommendations for CWs (both new and existing) to enhance their usefulness as newt-friendly habitats. Terrestrial habitats surrounding NWs and CWs were mapped using ArcGIS. Potential barriers to newt movement in addition to the presence of features such as wood or stone which could act as potential newt refuges were also mapped. Natural wetlands had significantly more terrestrial habitat types than CWs and while woodlands at both wetland types were most likely to contain features of benefit to newts, terrestrial habitats of NWs contained more features compared to those of CWs. The application of a Habitat Suitability Index, which assesses the likelihood of the presence of newts, resulted in seven of eight NWs compared to only two of eight CWs receiving “good” scores, the lower scores for CWs being due primarily to the presence of a barrier to newt movement. Recommendations for enhancing the design and management of CWs for smooth newts include less intensive ground maintenance, reduction of barriers to newt movement, judicious planting of suitable trees or shrubs and the provision of additional refuges such as wood or stone

Improving object segmentation by using EEG signals and rapid serial visual presentation

This paper extends our previous work on the potential of EEG-based brain computer interfaces to segment salient objects in images. The proposed system analyzes the Event Related Potentials (ERP) generated by the rapid serial visual presentation of windows on the image. The detection of the P300 signal allows estimating a saliency map of the image, which is used to seed a semi-supervised object segmentation algorithm. Thanks to the new contributions presented in this work, the average Jaccard index was improved from $0.47$ to $0.66$ when processed in our publicly available dataset of images, object masks and captured EEG signals. This work also studies alternative architectures to the original one, the impact of object occupation in each image window, and a more robust evaluation based on statistical analysis and a weighted F-score

Dextral and sinistral Amphidromus inversus (Gastropoda: Pulmonata: Camaenidae) produce dextral sperm

Coiling direction in pulmonate gastropods is determined by a single gene via a maternal effect, which causes cytoskeletal dynamics in the early embryo of dextral gastropods to be the mirror image of the same in sinistral ones. We note that pulmonate gastropod spermatids also go through a helical twisting during their maturation. Moreover, we suspect that the coiling direction of the helical elements of the spermatozoa may affect their behaviour in the female reproductive tract, giving rise to the possibility that sperm chirality plays a role in the maintenance of whole-body chiral dimorphism in the tropical arboreal gastropod Amphidromus inversus (Müller, 1774). For these reasons, we investigated whether there is a relationship between a gastropod’s body chirality and the chirality of the spermatozoa it produces. We found that spermatozoa in A. inversus are always dextrally coiled, regardless of the coiling direction of the animal itself. However, a partial review of the literature on sperm morphology in the Pulmonata revealed that chiral dimorphism does exist in certain species, apparently without any relationship with the coiling direction of the body. Though our study shows that body and sperm chirality follows independent developmental pathways, it gives rise to several questions that may be relevant to the understanding of the chirality of spermatid ultrastructure and spermatozoan motility and sexual selection

Mycolactone-dependent depletion of endothelial cell thrombomodulin is strongly associated with fibrin deposition in Buruli ulcer lesions

A well-known histopathological feature of diseased skin in Buruli ulcer (BU) is coagulative necrosis caused by the Mycobacterium ulcerans macrolide exotoxin mycolactone. Since the underlying mechanism is not known, we have investigated the effect of mycolactone on endothelial cells, focussing on the expression of surface anticoagulant molecules involved in the protein C anticoagulant pathway. Congenital deficiencies in this natural anticoagulant pathway are known to induce thrombotic complications such as purpura fulimans and spontaneous necrosis. Mycolactone profoundly decreased thrombomodulin (TM) expression on the surface of human dermal microvascular endothelial cells (HDMVEC) at doses as low as 2ng/ml and as early as 8hrs after exposure. TM activates protein C by altering thrombin's substrate specificity, and exposure of HDMVEC to mycolactone for 24 hours resulted in an almost complete loss of the cells' ability to produce activated protein C. Loss of TM was shown to be due to a previously described mechanism involving mycolactone-dependent blockade of Sec61 translocation that results in proteasome-dependent degradation of newly synthesised ER-transiting proteins. Indeed, depletion from cells determined by live-cell imaging of cells stably expressing a recombinant TM-GFP fusion protein occurred at the known turnover rate. In order to determine the relevance of these findings to BU disease, immunohistochemistry of punch biopsies from 40 BU lesions (31 ulcers, nine plaques) was performed. TM abundance was profoundly reduced in the subcutis of 78% of biopsies. Furthermore, it was confirmed that fibrin deposition is a common feature of BU lesions, particularly in the necrotic areas. These findings indicate that there is decreased ability to control thrombin generation in BU skin. Mycolactone's effects on normal endothelial cell function, including its ability to activate the protein C anticoagulant pathway are strongly associated with this. Fibrin-driven tisischemia could contribute to the development of the tissue necrosis seen in BU lesions