Infection levels of proteocephalidean cestodes in Cichla piquiti (Osteichthyes: Cichlidae) of the Volta Grande Reservoir, Minas Gerais, Brazil, relative to host body weight and gender

We evaluated the relationship between infection by proteocephalid cestodes and the sex and weight classes of tucunaré (Cichla piquiti) captured between August 1999 and June 2001 in the Volta Grande Reservoir, Minas Gerais, Brazil. A total of 96 fish, 75.9±9.3% males and 88.9±6.4% females, were parasitized by Proteocephalus macrophallus and P. microscopicus, with total mean intensities of 76.6±23.9 and 145.2±36.7, respectively, during this period. In the majority of the months analysed, males showed 71.4-100% prevalence of parasitism and females 80-100%. Although there was no significant difference, females showed a higher mean intensity of infection (145.2±36.7) than males (76.6±23.9). Fish weighing 300-800g showed a higher mean abundance of parasites (P<0.05) compared with the biggest specimens weighing 801-2750g. Analysing both males and females together, the greatest mean intensities of infection were found in October and December (P<0.05) independent of the year, which coincides with the months of highest rainfall. These results show that fish living in reservoirs may be more susceptible to intermediate hosts than those that live in river

Towards optimisation of surface enhanced photodynamic therapy of breast cancer cells using gold nanoparticle-photosensitiser conjugates

Gold nanoparticles (AuNPs; ca. 4 nm) were synthesised and functionalised with a mixed monolayer of polyethylene glycol (PEG) and one of two zinc phthalocyanines (ZnPcs), the difference between the two molecules was the length of the carbon chain that connects the Pc to the gold core. The chain was composed of either three (C3Pc) or eleven (C11Pc) carbon atoms. The C11Pc photosensitiser displayed higher fluorescence emission intensity than the C3Pc in solution. By contrast, the C3Pc photosensitiser exhibited higher fluorescence when bound to the surface of the AuNPs than the C11Pc, despite the shorter carbon chain which was expected to quench the fluorescence. In addition, the C3Pc nanoparticle conjugates exhibited an enhancement in the production of singlet oxygen (1O2). The metal-enhanced 1O2 production led to a remarkable photodynamic efficacy for the treatment of human breast cancer cells

Observations of Toroidal Coupling for Low-N Alfven Modes in the Tca Tokamak

The antenna structure in the TCA tokamak is phased to excite preferentially Alfven waves with known toroidal and poloidal wave numbers. Surprisingly, the loading spectrum includes both discrete and continuum modes with poloidal wave numbers incompatible with the antenna phasing. These additional modes, which are important for our heating experiments, can be attributed to linear mode coupling induced by the toroidicity of the plasma column, when we take into account ion-cyclotron effects

Targeted photodynamic therapy of breast cancer cells using lactose-phthalocyanine functionalized gold nanoparticles

Gold nanoparticles (AuNPs), which have been widely used for the delivery of photosensitizers for photodynamic therapy (PDT) of cancer, can be dispersed in aqueous solutions, improving the delivery of the hydrophobic photosensitizer into the body. Furthermore, the large surface of AuNPs can be functionalized with a variety of ligands, including proteins, nucleic acids and carbohydrates, that allow selective targeting to cancer tissue. In this study, gold nanoparticles were functionalized with a mixed monolayer of a zinc phthalocyanine and a lactose derivative. For the first time, a carbohydrate was used with a dual purpose, as the stabilizing agent of the gold nanoparticles in aqueous solutions and as the targeting agent for breast cancer cells. The functionalization of the phthalocyanine-AuNPs with lactose led to the production of water-dispersible nanoparticles that are able to generate singlet oxygen and effect cell death upon irradiation. The targeting ability of lactose of the lactose-phthalocyanine functionalized AuNPs was studied in vitro towards the galectin-1 receptor on the surface of breast cancer cells. The targeting studies showed the exciting potential of lactose as a specific targeting agent for galactose-binding receptors overexpressed on breast cancer cells

Compact modeling of organic thin film transistors with solution processed octadecyl substituted tetrabenzotriazaporphyrin as an active layer

Using 70nm thick spin-coated film of newly synthesized octadecyl substituted copper tetrabenzotriazaporphyrin (10CuTBTAP) as an active layer on a highly doped silicon (110) gate electrode substrates, output characteristics and transfer characteristics of bottom-gate bottom-contact organic thin film transistors have been measured at room temperature. A compact model for thin film transistors has been employed as a part of circuit design tool to extract device parameters such as the charge carrier mobility μ, the threshold voltage VT and the contact resistances. Parallel measurements and analysis were performed on similarly constructed devices with a copper phthalocyanine analogue (10CuPc). The results reveal that the 10CuPc layer is relatively more susceptible to trapping degradation near the gate region than a 10CuTBTAP layer, which is significant in order to achieve stability in these transistors. The application of the simple square law in the classical MOS model provides a quicker but approximate interpretation of the transistor performance without providing information on the gate voltage dependence of mobility and the effects of the contact regions. In this comparative study, the analysis of the contact regions is found to be very important for determining the difference in the performance of two transistors

Steady state charge conduction through solution processed liquid crystalline lanthanide bisphthalocyanine films

In-plane electrical characteristics of non-peripherally octyl (C8H17) and hexyl (C6H13) substituted liquid crystalline (LC) double decker lanthanide bisphthalocyanine (LnPc2) complexes with central metal ions lutetium (Lu), and gadolinium (Gd) have been measured in thin film formulations on interdigitated gold (Au) electrodes for the applied voltage (V_a) range of 〖0 ≤ V〗_a ≤100 V. The conduction mechanism is found to be Ohmic within the bias of 〖0 ≤ V〗_a ≤30 V 0≤Va≤30 V while the bulk limited Poole-Frenkel mechanism is responsible for the higher bias. The compounds show individual characteristics depending on the central metal ions, substituent chain lengths and their mesophases. Values of 67.55 μS〖cm〗^(-1) and 42.31 μS〖cm〗^(-1) have been obtained. for room temperature in-plane Ohmic conductivity of as-deposited octyl lutetium (C8LuPc2) and hexyl gadolinium (C6GdPc2) films, respectively while C8GdPc2 films exhibit nearly two orders of magnitude smaller conductivity. On annealing at 80 ̊C, Ohmic conductivities of C8LuPc2 and C8GdPc2 are found to have increased but the conductivity of C6GdPc2 decreases by more than one order of magnitude to 1.5 μS〖cm〗^(-1). For physical interpretation of the charge transport behavior of these three molecules, their UV-visible optical absorption spectra have been studied in the solution and in as-deposited and annealed solid phases. It is believed that both orientational and positional reorganisations are responsible depending upon the size of the central ion and side chain length

Cancer targeting with biomolecules: a comparative study of photodynamic therapy efficacy using antibody or lectin conjugated phthalocyanine-PEG gold nanoparticles

The functionalisation of therapeutic nanoparticle constructs with cancer-specific biomolecules can enable selective tumour accumulation and targeted treatment. Water soluble gold nanoparticles (ca. 4 nm) stabilised by a mixed monolayer of a hydrophobic zinc phthalocyanine photosensitiser (C11Pc) and hydrophilic polyethylene glycol (PEG) have been prepared. The C11Pc-PEG gold nanoparticle constructs were further functionalised with jacalin, a lectin specific for the cancer-associated Thomsen–Friedenreich (T) carbohydrate antigen, or with monoclonal antibodies specific for the human epidermal growth factor receptor-2 (HER-2). The two biofunctionalised nanoparticle conjugates produced similar levels of singlet oxygen upon irradiation at 633 nm. Importantly, both nanoparticle conjugates demonstrated extensive, yet comparable, phototoxicity in HT-29 colorectal adenocarcinoma cells (80–90%) and in SK-BR-3 breast adenocarcinoma cells (>99%). Non-conjugated C11Pc-PEG gold nanoparticles were only minimally phototoxic. Lysosomal colocalisation studies performed with the HT-29 colon cancer cells and the SK-BR-3 breast cancer cells revealed that both nanoparticle conjugates were partially localised within acidic organelles, which is typical of receptor-mediated endocytosis. The similarity of the targeted PDT efficacy of the two biofunctionalised C11Pc-PEG gold nanoparticles is discussed with respect to targeting ligand binding affinity and cell surface antigen density as key determinants of targeting efficiency. This study highlights how targeting small cell-surface molecules, such as the T antigen, can mediate a selective photodynamic treatment response which is similar to that achieved when targeting overexpressed protein receptors, such as HER-2. The high prevalence of the T antigen present on the cellular surface of primary tumours emphasises the broad potential applications for lectin-targeted therapies