Porcine to Human Heart Transplantation: Is Clinical Application Now Appropriate?

Cardiac xenotransplantation (CXTx) is a promising solution to the chronic shortage of donor hearts. Recent advancements in immune suppression have greatly improved the survival of heterotopic CXTx, now extended beyond 2 years, and life-supporting kidney XTx. Advances in donor genetic modification (B4GALNT2 and CMAH mutations) with proven Gal-deficient donors expressing human complement regulatory protein(s) have also accelerated, reducing donor pig organ antigenicity. These advances can now be combined and tested in life-supporting orthotopic preclinical studies in nonhuman primates and immunologically appropriate models confirming their efficacy and safety for a clinical CXTx program. Preclinical studies should also allow for organ rejection to develop xenospecific assays and therapies to reverse rejection. The complexity of future clinical CXTx presents a substantial and unique set of regulatory challenges which must be addressed to avoid delay; however, dependent on these prospective life-supporting preclinical studies in NHPs, it appears that the scientific path forward is well defined and the era of clinical CXTx is approaching

Healthcare personnel and hand decontamination in intensive care units: Knowledge, attitudes, and behaviour in Italy

The purpose of this study was to evaluate knowledge, attitudes, and behaviour regarding hand decontamination in personnel of intensive care units (ICUs) in Italy. All ICU physicians and nurses in 19 and five randomly selected hospitals in Campania and Calabria (Italy) were mailed a questionnaire focusing on demographics and practice characteristics; knowledge about prevention of hospital acquired infection; attitudes and behaviour with respect to hand decontamination; and use of gloves. A total of 413 questionnaires were returned giving a response rate of 66.6%. Overall, 53.2% agreed with the correct responses on knowledge related to infection control, and this knowledge was significantly higher in neonatal and medicine-surgery wards and in larger ICUs. A positive attitude was reported by the large majority who agreed that hand decontamination reduces the risk of infection in patients (96.8%) and personnel (86.2%), and the positive attitude was significantly higher among older and female personnel and in those with a higher level of knowledge. Only 60% always decontaminate hands at the start of a shift, and 72.5% before and after a patient contact. Higher compliance is reported for invasive manoeuvres, such as urinary catheters (96.5%) and intravenous lines (77.1%). Routine hand decontamination between each patient was significantly higher in females, and in neonatal and medicine-surgery ICUs. Our results suggest that interventions should not only be focused on predisposing factors (knowledge), but also on enabling (facilitating) and reinforcing (gratifying) factors

Studi Fenofisiologi Pembungaan Salak Gula Pasir Sebagai Upaya Mengatasi Kegagalan Fruit-Set

. Rai, I. N., C.G.A. Semarajaya, and I. W. Wiraatmaja. 2010. A Study on the FloweringPhenophysiology of Gula Pasir Snake Fruit to Prevent Failure of Fruit-set. Flowering of Gula Pasir snake fruitnaturally occures once every 3 months or four times a year, but only one to two of flowering seasons succeeds toproduce fruit. Fruit-set failure causes the fruit harvest seasonally occurs. This research was aimed to study Floweringphenophysiology of Gula Pasir snake fruit to prevent failure fruit-set and to produce off-season fruit. A randomizedcomplete design with one treatment factor and 20 replications was used in the research. The treatment was floweringseason consisted of three levels i.e. sela I (April), gadu (July), and sela II (October). The research was conductedat a farmer's snake fruit orchard in Sibetan Village, Bebandem Subdistrict, Karangasem Regency, from March toNovember 2009. Variables observed were flower and fruit growth, microclimate, N, P, and K leaf tissue content,and N, P, and K soil content. The data were analyzed by analysis of variance (anova). The results showed that theability of the crop to flowering was the same between season of sela I, gadu, and sela II. However, the percentageof fruit-set at gadu was very low (20.53%) or flower drop was very high (79.47%). Due to unfavorable climaticecondition that was very low total rain day and rainfall. Therefore, the crop did not obtain sufficient water, that wasindicated by the lowest relative water content on leaf. Off-season fruit production of Gula Pasir snake fruit of couldbe established by decreasing the percentage of flower drop by utilyzing irrigation as the substitution of low totalrain day and rainfall

Upper atmospheres and ionospheres of planets and satellites

The upper atmospheres of the planets and their satellites are more directly exposed to sunlight and solar wind particles than the surface or the deeper atmospheric layers. At the altitudes where the associated energy is deposited, the atmospheres may become ionized and are referred to as ionospheres. The details of the photon and particle interactions with the upper atmosphere depend strongly on whether the object has anintrinsic magnetic field that may channel the precipitating particles into the atmosphere or drive the atmospheric gas out to space. Important implications of these interactions include atmospheric loss over diverse timescales, photochemistry and the formation of aerosols, which affect the evolution, composition and remote sensing of the planets (satellites). The upper atmosphere connects the planet (satellite) bulk composition to the near-planet (-satellite) environment. Understanding the relevant physics and chemistry provides insight to the past and future conditions of these objects, which is critical for understanding their evolution. This chapter introduces the basic concepts of upper atmospheres and ionospheres in our solar system, and discusses aspects of their neutral and ion composition, wind dynamics and energy budget. This knowledge is key to putting in context the observations of upper atmospheres and haze on exoplanets, and to devise a theory that explains exoplanet demographics.Comment: Invited Revie

A multi-gene signature predicts outcome in patients with pancreatic ductal adenocarcinoma.

© 2014 Haider et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.Improved usage of the repertoires of pancreatic ductal adenocarcinoma (PDAC) profiles is crucially needed to guide the development of predictive and prognostic tools that could inform the selection of treatment options

Markers for the identification of late breast cancer recurrence

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited

Modelling the upper atmosphere of the gas giant planets

At Jupiter and Saturn the thermosphere is the region of the neutral atmosphere that coexists with the ionosphere. It is thus the region of the atmosphere that is most strongly coupled to the magnetosphere, and is responsible via the ionosphere for the transfer of planetary angular momentum to the magnetosphere. Both planets also exhibit high thermospheric temperatures that are yet to be explained. We study the coupled thermosphere-ionosphere-magnetosphere systems of Jupiter and Saturn using a thermospheric general circulation model and simple models of the ionosphere and magnetosphere. Our principle result is that meridional winds in the thermosphere are of critical importance to the interaction. Angular momentum extracted from the thermosphere by magnetospheric drag is found to be replaced largely by meridional advection, not, as commonly supposed, by vertical viscous transfer. These same meridional winds are also able to couple together regions of the magnetosphere that otherwise would not interact. We find it very hard to reproduce the observed thermospheric temperatures with our model. Under a limited range of circumstances it is shown that redistribution of thermal energy from high- to low-latitudes by winds can explain the available observations. However, the inclusion of ion drag generates a circulation in the polar regions that acts as a heat pump and efficiently cools the thermosphere, significantly reducing the efficiency of the redistributive winds

The politics of protest in newspaper campaigns: dissent, populism and the rhetoric of authenticity

Newspaper campaigns embody newspaper’ most emphatic claims to speak for ‘the people’, and as such are generally regarded as populist. However, they can be oppositional, engaging in dissent of one sort or another, and often assume a certain amount of political engagement with that dissent on the part of the audience. This article examines the potential of newspaper camapigns to facilitate the political engagement of citizens through the politics of protest. It draws on qualitative analysis of seven campaigns that ran in the Scottish press between 2000 and 2005, and semi-structured interviews with relevant journalists. The distinction between legitimate protest and manipulative populism is made in terms of: (a) the rhetoric and strategies of political representation, participation and influence and (b) the construction of political legitimacy in terms of the public interest and the moral authority of the ‘victim’. It is argued that populist impulses dominate, driving a tendency to use discourses of emotional authenticity and offence to legitimise demands for a plebiscitary response to popular of ‘victim’ preference and to close down controversy and debate, with the principal objective marketing the newspaper as an influential community champion

Axial symmetry breaking of Saturn's thermosphere

The source of the various planetary-period signals in Saturn’s magnetosphere is at present unknown. We investigate the possibility that the source of these signals is an axially asymmetric wind system in the thermosphere. We describe a feedback mechanism that has the potential to drive such axially asymmetric wind systems. The proposed mechanism relates thermospheric winds to heating from particle precipitation, via the generation of horizontal and field-aligned currents. The relevant physical processes are investigated using a highly simplified general circulation model of Saturn’s thermosphere and ionosphere. Our principal result is that the feedback mechanism is effective in permanently breaking the axial symmetry of the thermosphere, generating a drifting vortex-like structure at high latitudes. However, the precipitating electron energies required to power this structure are of the order of 5 MeV, 2–3 orders of magnitude greater than the observed auroral electron energies, and the highly axially asymmetric distribution of precipitation required across the polar regions of the planet is also inconsistent with observations. Despite these flaws, the model qualitatively explains several features of the observed variation in the pulsing of SKR emissions; in particular, the seasonal variation and the faster rotation rate in the winter hemisphere. We cannot reproduce the apparent 7 month lag in the response of the Saturn Kilometric Radiation (SKR) rotation rate to seasonal variation, but instead suggest the possibility that this effect may have its origin in long chemical time-scales in the upper atmosphere. We also predict the possible existence of secondary periodic features in the SKR emissions with periods of ∼15 planetary rotations, driven by complex wave behaviour in the thermosphere

Apparent correlation of palaeomagnetic intensity and climatic records in deep-sea sediments

Most reports of a correlation between Pleistocene climate and geomagnetic field intensity rely strongly on the assumption that sediment natural remanent magnetic (NRM) intensity provides a record of geomagnetic field strength and is not sensitive to local changes in properties of the sediment. Critical assessment of relevant data presented here and elsewhere from deep-sea sediment cores shows that a pronounced dependence of NRM intensity on sediment composition can occur which implies that this assumption is unlikely to be generally valid. As sediment composition often reflects varying depositional conditions induced by climatic change, the significance of correlations proposed between Pleistocene palaeomagnetism and climatic indicators in deep-sea sediments may be less dramatic than sometimes supposed