71 research outputs found

    Probing the roles of LRR RLK genes in Arabidopsis thaliana roots using a custom T-DNA insertion set

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    Leucine-rich repeat receptor-like protein kinases (LRR RLKs) represent the largest group of Arabidopsis RLKs with approximately 235 members. A minority of these LRR RLKs have been assigned to diverse roles in development, pathogen resistance and hormone perception. Using a reverse genetics approach, a collection of homozygous T-DNA insertion lines for 69 root expressed LRR RLK genes was screened for root developmental defects and altered response after exposure to environmental, hormonal/chemical and abiotic stress. The obtained data demonstrate that LRR RLKs play a role in a wide variety of signal transduction pathways related to hormone and abiotic stress responses. The described collection of T-DNA insertion mutants provides a valuable tool for future research into the function of LRR RLK genes

    Novel Disease Susceptibility Factors for Fungal Necrotrophic Pathogens in Arabidopsis

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    Host cells use an intricate signaling system to respond to invasions by pathogenic microorganisms. Although several signaling components of disease resistance against necrotrophic fungal pathogens have been identified, our understanding for how molecular components and host processes contribute to plant disease susceptibility is rather sparse. Here, we identified four transcription factors (TFs) from Arabidopsis that limit pathogen spread. Arabidopsis mutants defective in any of these TFs displayed increased disease susceptibility to Botrytis cinerea and Plectosphaerella cucumerina, and a general activation of non-immune host processes that contribute to plant disease susceptibility. Transcriptome analyses revealed that the mutants share a common transcriptional signature of 77 up-regulated genes. We characterized several of the up-regulated genes that encode peptides with a secretion signal, which we named PROVIR (for provirulence) factors. Forward and reverse genetic analyses revealed that many of the PROVIRs are important for disease susceptibility of the host to fungal necrotrophs. The TFs and PROVIRs identified in our work thus represent novel genetic determinants for plant disease susceptibility to necrotrophic fungal pathogens.Funding: This work was supported by the Spanish MINECO (BFU2012 to PV), and Generalitat Valenciana (Prometeo2014/020 to PV). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Dobón Alonso, A.; Canet Perez, JV.; García-Andrade Serrano, J.; Angulo, C.; Neumetzler, L.; Persson, S.; Vera Vera, P. (2015). Novel Disease Susceptibility Factors for Fungal Necrotrophic Pathogens in Arabidopsis. PLoS Pathogens. 11(4):1-30. https://doi.org/10.1371/journal.ppat.1004800S13011

    Il testo poetico

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    Introduzione allo studio della poesia europea, con testi nelle varie lingue, analisi critiche, spiegazioni dei termini tecnici, ecc

    La riproduzione tecnica delle storie e dei miti classici

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    Il posto dell'antichit\ue0 classica nella cultura postmoderna e nei sistemi educativi di oggi.I meccanismi del mercato. Un esempio: i racconti di Guy Davenport

    Os sonhos vis\uedves de Calvino

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    The theme of dreams in the work of Italo Calvino. Examples and significance. Melancholy and irony in his treatment of dreams

    Body and Soul in Romantic Love, in ACT 9: Corpo e Paisagem Rom\ue2nticos, a cura di H. Carvalh\ue2o Buescu, J. Ferreira Duarte e F. Fernandes da Silva, Lisboa, Edi\ue7\uf0es Colibri, pp. 15-37.

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    The model of Romantic Love: nody and soul in a communion. Examples from European Literature: Benjamin Constant, Stendhal, Foscolo, Tolstoj

    Il contagio e la letteratura postmoderna

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    Il tema del contagio nella letteratura postmoderna. L'esempio del romanzo di Don DeLillo White Noise. Analisi dei temi del romanzo

    Introducci\uf3n a los estudios literarios

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    E' la traduzione spagnola della Guida breve allo studio della letteratura, pubblicata in Italia da Laterza nel 2003. E' un'introduzione ai vari metodi di studio della letteratura europea, con molti esempi di interpretazione di testi, discussione delle scuole critiche, bibligrafie
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