Changes in the fecal microbiota associated with a broad‐spectrum antimicrobial administration in hospitalized neonatal foals with probiotics supplementation

There is a wide array of evidence across species that exposure to antibiotics is associated with dysbiosis, and due to their widespread use, this also raises concerns also in medicine. The study aimed to determine the changes on the fecal microbiota in hospitalized neonatal foals administered with broad‐spectrum antimicrobials and supplemented probiotics. Fecal samples were collected at hospital admission (Ta), at the end of the antimicrobial treatment (Te) and at discharge (Td). Feces were analysed by next‐generation sequencing of the 16S rRNA gene on Illumina MiSeq. Seven foals treated with IV ampicillin and amikacin/gentamicin were included. The mean age at Ta was 19 h, the mean treatment length was 7 days and the mean time between Te and Td was 4.3 days. Seven phyla were identified: Actinobacteria, Bacteroidetes, Firmicutes, Fusobacteria, Proteobacteria, TM7 and Verrucomicrobia. At Ta, Firmicutes (48.19%) and Proteobacteria (31.56%) were dominant. The alpha diversity decreased from Ta to Te, but it was the highest at Td. The beta diversity was higher at Ta than at Te and higher at Td than at Te. An increase in Akkermansia over time was detected. The results suggest that the intestinal microbiota of neonatal foals rapidly returns to a high diversity after treatment. It is possible that in foals, the effect of antimicrobials is strongly influenced or overshadowed by the time‐dependent changes in the developing gut microbiota

And Yet they Co-Move! Public Capital and Productivity in OECD.

In this paper we add to the debate on the public capital-productivity link by applying very recent developments in the panel time series literature that take into account cross sectional correlation in non-stationary panels. In particular we evaluate the productive effect of public capital by estimating various production functions on a panel of 21 OECD countries over the period 1975-2002. Our results suggest that public capital has a positive long run impact on output, with elasticities that range between 0.05-0.15, depending on model specification. These findings are robust to the existence of spillover effects from public capital investments in other countries and to the inclusion of other productivity determinants, like human capital, the stock of patents and R&D capital. Finally, we do not find any important effect of public capital on GDP in the short run: this suggests that public infrastructure investments might not be a powerful countercyclical policy instrument

A Comparison between Rotating Squares and Anti-Tetrachiral Systems: Influence of Ligaments on the Multi-Axial Mechanical Response

Rotating unit systems are one of the most important and well-known classes of auxetic mechanical metamaterials. As their name implies, when loaded, these systems deform primarily via rotation of blocks of material, which may be connected together either directly through joints (or ‘joint-like’ connections made by overlapping vertices of the rotating units) as in the case of rotating rigid polygonal-unit systems or by ligaments/ribs as in the case of chiral honeycombs. In this work, we used Finite Element Analysis to investigate the effect which the presence/absence of ligaments has on the on-axis and off-axis mechanical properties of these systems by analysing two of the most well-known structures which characterise these two cases: the rotating square system and the anti-tetrachiral honeycomb. It was found that while the presence of ligaments has a negligible effect on the on-axis Poisson’s ratio of these systems, it has a profound influence on nearly all other mechanical properties as well as on the off-axis loading behaviour. Systems with ligaments were found to exhibit a high level of anisotropy and also a severely reduced level of stiffness in comparison to their non-ligamented counterparts. On the other hand, the rotating square system suffers from high localized stress-intensities and has a very low strain-tolerance threshold. In addition, an optimized ‘hybrid’ geometry which is specifically designed to capture the best features of both the anti-tetrachiral and rotating square system, was also analysed. This work shows the main differences between ligament-based and non-ligament-based auxetic structures and also highlights the importance of considering the off-axis mechanical response in addition to the on-axis properties when investigating such systems

Modellazione efficiente agli elementi finiti per l’analisi a collasso di strutture incollate complesse

Il lavoro verifica l’applicabilità di un modello semplificato agli elementi finiti per l’analisi a collasso post elastico di strutture incollate complesse in parete sottile. Al fine di superare le limitazioni dei modelli di letteratura come l’uso di elementi speciali, il lavoro sfrutta un modello ridotto già presentato dagli autori in campo elastico. Tale modello è basato sulla rappresentazione degli aderendi mediante elementi semistrutturali (piastre o gusci) e dell’adesivo per mezzo di speciali elementi coesivi. La continuità strutturale tra aderendi e adesivo è ottenuta mediante vincoli interni (tied mesh) che accomunano i gradi di libertà dei nodi mutuamente affacciati di aderendi ed adesivo. La struttura analizzata è un simulacro di incollaggio industriale e produce nella strato adesivo una sollecitazione complessa, analizzabile solo con modelli numerici. Si considera una struttura tubolare in parete sottile a sezione quadrata, fatta di due spezzoni posti testa a testa e incollati con fazzoletti di lamiera sui quattro lati. La struttura è sottoposta a flessione a tre punti fino al cedimento e la zona incollata posta disassata rispetto al punto di applicazione del carico riceve una sollecitazione indiretta. I risultati dell’analisi FEM, confrontati direttamente con le curve sperimentali forza-spostamento, evidenziano una buona accuratezza del metodo, in termini di rigidezza, forza massima e comportamento post elastico della struttura, accompagnati da ridotte dimensioni del modello e tempi di calcolo molto contenuti. Grazie a questi vantaggi, la procedura si presta ad effettuare l’analisi di strutture incollate complesse, altrimenti ingestibili se affrontate con una modellazione agli elementi finiti tradizionale

Experimental characterization of pull-in parameters for an electrostatically actuated cantilever

MEMS-NEMS applications extensively use micro-nano cantilever structures as actuation system, thanks to their intrinsically simple end efficient configuration. Under the action of an electrostatic actuation voltage the can- tilever deflects, until it reaches the maximum value of the electrostatic actuation voltage, namely the pull-in voltage. This limits its operating point and is a critical issue for the switching of the actuator. The present work aims to experimentally measure the variation of the pull-in voltage and the tip deflection for different geometri- cal parameters of an electrostatically actuated cantilever. First, by relying on a nonlinear differential model from the literature, we designed and built a macro-scale cantilever switch, which can be simply adapted to different configurations. Second, we experimentally investigated the effect of the free length of the suspended electrode, and of the gap from the ground, on the pull-in response. The experimental results always showed a close agree- ment with the analytical predictions, with a maximum relative error lower that 10% for the pull-in voltage, and a relative difference lower than 18% for the pull-in deflection

Adjustment and transformation strategies of ItalPoS Permanent GNSS Network

A private network of 133 Global Navigation Satellite Systems (GNSS) permanent sites, named ItalPoS (Italian Positioning Service) and uniformly covering the entire Italian territory, was established in April 2006 by the Italian Division of Leica Geosystems S.p.A. This network also involves several GPS stations of the INGV (Italian National Institute of Geophysics and Volcanology) RING (Real-time Integrated National Gps) network and GPS stations from other public and private bodies. Indeed Leica Geosystems contributed with its experience and its products to these networks which currently fulfil the national coverage where there are no ItalPoS stations. For instance, the purpose of the ItalPoS network is mainly GNSS data distribution in terms of real time corrections and RINEX files for the post-processing. In order to compute and distribute to private and scientific users a set of adjusted coordinates of the ItalPoS network in the IGS05 and ETRF89-2000 reference frames, a dataset of RINEX of about 120 GPS permanent sites was created and processed, using the distributed sessions approach, by means of Gamit/Globk 10.3 GPS data processor, also using some SOPAC (Scripps Orbits and Permanent Array Center) and EUREF (European Permanent Network) solutions to improve the reference frame definition and the internal constraints. This paper describes all the strategies adopted to estimate coordinates and velocities of the ItalPoS Network in IGS05 and in ETRF89-2000 reference frames in the whole Italian territory