2 research outputs found
Additively Manufactured Macroporous Titanium with Silver-Releasing Micro-/Nanoporous Surface for Multipurpose Infection Control and Bone Repair – A Proof of Concept
Restoring
large-scale bone defects, where osteogenesis is slow while infections
lurk, with biomaterials represents a formidable challenge in orthopedic
clinics. Here, we propose a scaffold-based multipurpose anti-infection
and bone repairing strategy to meet such restorative needs. To do
this, personalized multifunctional titanium meshes were produced through
an advanced additive manufacturing process and dual “TiO<sub>2</sub>–polyÂ(dopamine)/Ag (nano)” post modifications,
yielding macroporous constructs with micro-/nanoporous walls and nanosilver
bullets immobilized/embedded therein. Ultrahigh loading capacity and
durable release of Ag<sup>+</sup> were accomplished. The scaffolds
were active against planktonic/adherent bacteria (Gram-negative and
positive) for up to 12 weeks. Additionally, they not only defended
themselves from biofilm colonization but also helped destroy existing
biofilms, especially in combination with antibiotics. Further, the
osteoblasts/bacteria coculture study displayed that the engineered
surfaces aided MG-63 cells to combat bacterial invasion. Meanwhile,
the scaffolds elicited generally acceptable biocompatibility (cell
adhesion, proliferation, and viability) and hastened osteoblast differentiation
and maturation (alkaline phosphatase production, matrix secretion,
and calcification), by synergy of micro-/nanoscale topological cues
and bioactive catecholamine chemistry. Although done ex vivo, these
studies reveal that our three-in-one strategy (infection prophylaxis,
infection fighting, and bone repair) has great potential to simultaneously
prevent/combat infections and bridge defected bone. This work provides
new thoughts to the use of enabling technologies to design biomaterials
that resolve unmet clinical needs