Dataset on fabrication of an improved L-lactate biosensor based on lactate oxidase/cMWCNT/CuNPs/PANI modified PG electrode

The data shown in this article are based on the original research article entitled “An improved amperometric L-lactate biosensor based on covalent immobilization of microbial lactate oxidase onto carboxylated multiwalled carbon nanotubes/copper nanoparticles/ polyaniline modified pencil graphite electrode” (Dagar and Pundir, 2017) [1]. This article explains the fabrication of an amperometric L-lactate biosensor based on microbial lactate oxidase (LOx) covalent immobilization onto nanomatrix [(carboxylated multiwalled carbon nanotubes (cMWCNT)/copper nanoparticles (CuNPs)/polyaniline (PANI) hybrid film/pencil graphite electrode (PGE)]. The dataset based on this article is made publically available for critical analysis. The whole data is supplied in the research article instead of repository. The data in the article is not related to any already published article. Keywords: L-Lactate oxidase, Nanomaterials, Lactic acid, Plasma, Pencil graphite electrode, Covalent bindin

Preparation of Oxygen Meter Based Biosensor for Determination of Triglyceride in Serum

A method is described for preparation of a dissolved oxygen meter (make Aqualytic, Germany) based triglyceride biosensor employing a polyvinyl chloride (PVC) membrane bound lipase, glycerol kinase (GK) and glycerol-3-phosphate oxidase The biosensor measures dissolved O2 utilized in the oxidation of triglyceride (TG) by membrane bound lipase, glycerol kinase (GK) and glycerol-3-phosphate oxidase (GPO), which is directly proportional to (TG) concentration. The biosensor showed optimum response within 10-15 sec at pH 7.5 and 39.5 ºC. A linear relationship was obtained between the (TG) concentration from 5mM to 20mM and oxygen consumed (mg/L). The biosensor was employed for determination of triglyceride in serum. The within and between batch coefficient of variation (CV) were < 2.18 % and < 1.7% respectively. The minimum detection limit of the biosensor was 0.35 mM. A study of interference revealed that ascorbic acid, cholesterol and bilirubin caused 13%, 15%, and 12% interference, respectively.The biosensor is portable and can be used outside the laboratory

An enhanced L-lactate biosensor based on nanohybrid of chitosan, iron-nanoparticles and carboxylated multiwalled carbon nanotubes

An enhanced biosensor was developed for the determination of blood lactate in lacto-acidosis patients. The biosensor employed a nanohybrid composed of chitosan/iron oxide nanoparticles and carboxylated multiwalled carbon nanotubes (CHIT/Fe3O4NPs/c-MWCNTs), electrodeposited onto an Au electrode, followed by covalent immobilization of L-lactate oxidase (LOx) onto this nano-hybrid. The biosensor (LOx/CHIT/Fe3O4NPs/c-MWCNTs/AuE) exhibited notable improvements in its analytical characteristics such as a rapid response time (4s), a lower detection limit of 0.15 μM and a wider linear range of 1–3000 μM of L-lactic acid. Additionally, it displayed enhanced reproducibility and an extended shelf life of 100 days. The biosensor was employed to measure the concentration of L-lactate in the plasma of both apparently healthy individuals and lacto-acidosis patients. The results showed that the L-lactate concentrations ranged from 112 ± 1.24 to 183 ± 29.15 μmol/L in apparently healthy individuals, whereas it ranged from 2236 ± 33.29 to 4949 ± 72.39 μmol/L in lacto-acidosis patients, which is significantly higher than in apparently healthy individuals. Thus, the integration of the CHIT/Fe3O4NPs/c-MWCNTs hybrid film in the biosensor led to the enhanced analytical performance of the biosensor

HER2 targeted noninvasive immunosensor based on pencil graphite electrode for detection of breast cancer

In the current research, we have fabricated an amperometric immunosensor for the detection of breast cancer using HER2 antibody. The sensor was fabricated by immobilization of anti-HER2 antibody on the surface of pencil graphite electrode (PGE). The techniques used for the characterization of the PGE/anti-HER2 were cyclic voltammetry, scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The sensor was optimized in terms of pH, temperature, antigen concentration, response time and antibody concentration. A linear range amidst 1 fgml−1 and 20 ngml−1 was observed. The limit of detection was 1 ​fg/ml. HER2 levels were also determined in sera samples of apparently healthy persons and breast cancer patients. The authentication of the fabricated immunosensor was noticeable due to its good regeneration ability and storage stability