Limnology in relation to fisheries in Tanzanian waters

The study of limnology is important to understand ecosystem dynamics and the ecological basis for fish production in the Lake Victoria which is important for fisheries resources use, planning and management. Physical, chemical and biological parameters are important and known to influence fish population production. Energy fixed by primary producers, e.g. algae, is transfered to higher trophic levels, e.g fish. Factors which influence the dynamics of phytoplankton and zooplankton population, e.g nutrient availability and uptake, growth rate, species composition and biomass, ultimately affect fish production. The commercial fisheries of Lake Victoria consists mainly of piscivorous Lates niloticus (L>), algivorous Oreochromis niloticus (L.) and zooplanktivorous Rastrineobola argentea (Pellegrin

Distribution and abundance of fish stocks in Lake Victoria, Tanzania

Trawl surveys to assess the stocks of Lake Victoria for estimates of biomass and yield, together with the establishment of exploitation patterns, are being undertaken under the Lake victoria Fisheries Research Project. Preliminary surveys to establish the sampling stations and strategy were carried out between October 1997 and February 1998. Three cruises to cover the whole of the Tanzanian waters were undertaken with a total of 133 sampling stations. Data on catch rates, species composition and distribution were collected

Aflatoxin in chili peppers in Nigeria: extent of contamination and control using atoxigenic aspergillus flavus genotypes as biocontrol agents

Open Access Journal; Published online: 22 July 2019Across sub-Saharan Africa, chili peppers are fundamental ingredients of many traditional dishes. However, chili peppers may contain unsafe aflatoxin concentrations produced by Aspergillus section Flavi fungi. Aflatoxin levels were determined in chili peppers from three states in Nigeria. A total of 70 samples were collected from farmers’ stores and local markets. Over 25% of the samples contained unsafe aflatoxin concentrations. The chili peppers were associated with both aflatoxin producers and atoxigenic Aspergillus flavus genotypes. E cacy of an atoxigenic biocontrol product, Aflasafe, registered in Nigeria for use on maize and groundnut, was tested for chili peppers grown in three states. Chili peppers treated with Aflasafe accumulated significantly less aflatoxins than nontreated chili peppers. The results suggest that Aflasafe is a valuable tool for the production of safe chili peppers. Use of Aflasafe in chili peppers could reduce human exposure to aflatoxins and increase chances to commercialize chili peppers in premium local and international markets. This is the first report of the e cacy of any atoxigenic biocontrol product for controlling aflatoxin in a spice crop

Assessment of aflatoxigenicAspergillusand other fungi in millet and sesame from Plateau State, Nigeria

Sixteen fonio millet and 17 sesame samples were analysed for incidence of moulds, especially aflatoxigenic Aspergillus species, in order to determine the safety of both crops to consumers, and to correlate aflatoxin levels in the crops with levels produced by toxigenic isolates on laboratory medium. Diverse moulds including Alternaria, Aspergillus, Cercospora, Fusarium, Mucor, Penicillium, Rhizopus and Trichoderma were isolated. Aspergillus was predominantly present in both crops (46–48%), and amongst the potentially aflatoxigenic Aspergillus species, A. flavus recorded the highest incidence (68% in fonio millet; 86% in sesame kernels). All A. parvisclerotigenus isolates produced B and G aflatoxins in culture while B aflatoxins were produced by only 39% and 20% of A. flavus strains isolated from the fonio millet and sesame kernels, respectively. Aflatoxin concentrations in fonio millet correlated inversely (r = −0.55; p = 0.02) with aflatoxin levels produced by toxigenic isolates on laboratory medium, but no correlation was observed in the case of the sesame samples. Both crops, especially sesame, may not be suitable substrates for aflatoxin biosynthesis. This is the first report on A. parvisclerotigenus in sesame

Fusarium : more than a node or a foot-shaped basal cell

Recent publications have argued that there are potentially serious consequences for researchers in recognising distinct genera in the terminal fusarioid clade of the family Nectriaceae. Thus, an alternate hypothesis, namely a very broad concept of the genus Fusarium was proposed. In doing so, however, a significant body of data that supports distinct genera in Nectriaceae based on morphology, biology, and phylogeny is disregarded. A DNA phylogeny based on 19 orthologous protein-coding genes was presented to support a very broad concept of Fusarium at the F1 node in Nectriaceae. Here, we demonstrate that re-analyses of this dataset show that all 19 genes support the F3 node that represents Fusarium sensu stricto as defined by F. sambucinum (sexual morph synonym Gibberella pulicaris). The backbone of the phylogeny is resolved by the concatenated alignment, but only six of the 19 genes fully support the F1 node, representing the broad circumscription of Fusarium. Furthermore, a re-analysis of the concatenated dataset revealed alternate topologies in different phylogenetic algorithms, highlighting the deep divergence and unresolved placement of various Nectriaceae lineages proposed as members of Fusarium. Species of Fusarium s. str. are characterised by Gibberella sexual morphs, asexual morphs with thin- or thick-walled macroconidia that have variously shaped apical and basal cells, and trichothecene mycotoxin production, which separates them from other fusarioid genera. Here we show that the Wollenweber concept of Fusarium presently accounts for 20 segregate genera with clear-cut synapomorphic traits, and that fusarioid macroconidia represent a character that has been gained or lost multiple times throughout Nectriaceae. Thus, the very broad circumscription of Fusarium is blurry and without apparent synapomorphies, and does not include all genera with fusarium-like macroconidia, which are spread throughout Nectriaceae (e.g., Cosmosporella, Macroconia, Microcera). In this study four new genera are introduced, along with 18 new species and 16 new combinations. These names convey information about relationships, morphology, and ecological preference that would otherwise be lost in a broader definition of Fusarium. To assist users to correctly identify fusarioid genera and species, we introduce a new online identification database, Fusarioid-ID, accessible at www.fusarium.org. The database comprises partial sequences from multiple genes commonly used to identify fusarioid taxa (act1, CaM, his3, rpb1, rpb2, tef1, tub2, ITS, and LSU). In this paper, we also present a nomenclator of names that have been introduced in Fusarium up to January 2021 as well as their current status, types, and diagnostic DNA barcode data. In this study, researchers from 46 countries, representing taxonomists, plant pathologists, medical mycologists, quarantine officials, regulatory agencies, and students, strongly support the application and use of a more precisely delimited Fusarium (= Gibberella) concept to accommodate taxa from the robust monophyletic node F3 on the basis of a well-defined and unique combination of morphological and biochemical features. This F3 node includes, among others, species of the F. fujikuroi, F. incarnatum-equiseti, F. oxysporum, and F. sambucinum species complexes, but not species of Bisifusarium [F. dimerum species complex (SC)], Cyanonectria (F. buxicola SC), Geejayessia (F. staphyleae SC), Neocosmospora (F. solani SC) or Rectifusarium (F. ventricosum SC). The present study represents the first step to generating a new online monograph of Fusarium and allied fusarioid genera (www.fusarium.org).http://www.studiesinmycology.org/BiochemistryForestry and Agricultural Biotechnology Institute (FABI)GeneticsMicrobiology and Plant PathologyPlant Production and Soil Scienc

Fusarium: more than a node or a foot-shaped basal cell

Recent publications have argued that there are potentially serious consequences for researchers in recognising distinct genera in the terminal fusarioid clade of the family Nectriaceae. Thus, an alternate hypothesis, namely a very broad concept of the genus Fusarium was proposed. In doing so, however, a significant body of data that supports distinct genera in Nectriaceae based on morphology, biology, and phylogeny is disregarded. A DNA phylogeny based on 19 orthologous protein-coding genes was presented to support a very broad concept of Fusarium at the F1 node in Nectriaceae. Here, we demonstrate that re-analyses of this dataset show that all 19 genes support the F3 node that represents Fusarium sensu stricto as defined by F. sambucinum (sexual morph synonym Gibberella pulicaris). The backbone of the phylogeny is resolved by the concatenated alignment, but only six of the 19 genes fully support the F1 node, representing the broad circumscription of Fusarium. Furthermore, a re-analysis of the concatenated dataset revealed alternate topologies in different phylogenetic algorithms, highlighting the deep divergence and unresolved placement of various Nectriaceae lineages proposed as members of Fusarium. Species of Fusarium s. str. are characterised by Gibberella sexual morphs, asexual morphs with thin- or thick-walled macroconidia that have variously shaped apical and basal cells, and trichothecene mycotoxin production, which separates them from other fusarioid genera. Here we show that the Wollenweber concept of Fusarium presently accounts for 20 segregate genera with clear-cut synapomorphic traits, and that fusarioid macroconidia represent a character that has been gained or lost multiple times throughout Nectriaceae. Thus, the very broad circumscription of Fusarium is blurry and without apparent synapomorphies, and does not include all genera with fusarium-like macroconidia, which are spread throughout Nectriaceae (e.g., Cosmosporella, Macroconia, Microcera). In this study four new genera are introduced, along with 18 new species and 16 new combinations. These names convey information about relationships, morphology, and ecological preference that would otherwise be lost in a broader definition of Fusarium. To assist users to correctly identify fusarioid genera and species, we introduce a new online identification database, Fusarioid-ID, accessible at www.fusarium.org. The database comprises partial sequences from multiple genes commonly used to identify fusarioid taxa (act1, CaM, his3, rpb1, rpb2, tef1, tub2, ITS, and LSU). In this paper, we also present a nomenclator of names that have been introduced in Fusarium up to January 2021 as well as their current status, types, and diagnostic DNA barcode data. In this study, researchers from 46 countries, representing taxonomists, plant pathologists, medical mycologists, quarantine officials, regulatory agencies, and students, strongly support the application and use of a more precisely delimited Fusarium (= Gibberella) concept to accommodate taxa from the robust monophyletic node F3 on the basis of a well-defined and unique combination of morphological and biochemical features. This F3 node includes, among others, species of the F. fujikuroi, F. incarnatum-equiseti, F. oxysporum, and F. sambucinum species complexes, but not species of Bisifusarium [F. dimerum species complex (SC)], Cyanonectria (F. buxicola SC), Geejayessia (F. staphyleae SC), Neocosmospora (F. solani SC) or Rectifusarium (F. ventricosum SC). The present study represents the first step to generating a new online monograph of Fusarium and allied fusarioid genera (www.fusarium.org)

Distribution of aflatoxigenic Aspergillus section Flavi in commercial poultry feed in Nigeria

The distribution and aflatoxigenicity of Aspergillus section Flavi isolates in 58 commercial poultry feed samplesobtained from 17 states in five agro-ecological zones (AEZs) in Nigeria were determined in order to assess thesafety of the feedswith respect to aflatoxin-producing fungi. Correlation was also performed for incidence of species,aflatoxin-producing ability of isolates in vitro, and aflatoxin (AFB1) concentrations in the feed. A total of1006 Aspergillus section Flavi isolates were obtained from 87.9% of the feed samples and identified as Aspergillusflavus, unnamed taxon SBG, Aspergillus parasiticus and Aspergillus tamarii. A. flavuswas themost prevalent (91.8%)of the isolates obtained from the feed in the AEZs while A. parasiticus had the lowest incidence (0.1%) and wasisolated only froma layer mash sample collected fromthe DS zone. About 29% of the Aspergillus isolates producedaflatoxins in maize grains at concentrations up to 440,500 ?g/kg B and 341,000 ?g/kg G aflatoxins. The incidenceof toxigenic isolates was highest (44.4%) in chick mash and lowest (19.9%) in grower mash. The population ofA. flavus in the feed had positive (r= 0.50) but non significant (p N 0.05) correlations with proportion of toxigenicisolates obtained fromthe feedwhile SBG had significant (p b 0.001) positive (r= 0.99)influence on AFB1 concentrationsin the feed. Poultry feed in Nigerian markets are therefore highly contaminated with aflatoxigenicAspergillus species and consequently, aflatoxins. This is a potential threat to the poultry industry and requires urgentintervention

The role of mycotoxin contamination in nutrition: The aflatoxin story

Over the past decade, there has been increasing recognition that the quantity of food alone guarantees neither food security nor adequate nutrition as measured by metrics such as hunger, malnutrition, and stunting. Increasingly, policy and decision makers understand the need to include nutritional aspects into improvements of food systems. However, not as fully recognized is that unsafe, contaminated foods thwart these efforts and maintain an unacceptable status quo in food insecurity, poverty, and a range of health-related problems. All of this makes sustainable development more challenging. In 2010, foodborne hazards caused 600 million illnesses and 420,000 deaths across the world, with 40 percent of this disease burden occurring among children under five years of age (Global Panel on Agriculture and Food Systems for Nutrition 2016). Yet food safety has become an important precondition for access to global food markets and, increasingly, for high-value domestic markets in developing countries. Contamination of food with mycotoxins is a prominent food safety challenge in tropical regions. In Africa, the most important mycotoxins from both a human health and an economic perspective are aflatoxins and fumonisins (IARC 2015). Much of the public- and private-sector’s attention has focused on aflatoxin due to its high pre- and postharvest contamination potential, which causes widespread occurrence in diverse food matrices, and its extreme toxicological significance to humans and animals, with impacts on food safety, nutrition, public health, and markets and income. Aflatoxin is a potent liver cancer–causing chemical, and there is mounting evidence that aflatoxin interferes with nutrient absorption and plays a role in inhibiting immune system function, potentially retarding child growth (Turner et al. 2012). With respect to food processing and trade, much of African produce is affected by aflatoxin, diminishing the region’s access to high-value export markets. Food-processing firms serving emerging domestic high-value markets are also testing for the contaminant in the production chain. This chapter focuses on the nutritional and economic consequences of aflatoxin contamination in Africa and on the opportunities for its management

Fungal and bacterial metabolites in commercial poultry feed from Nigeria

Metabolites of toxigenic fungi and bacteria occur as natural contaminants (e.g. mycotoxins) in feedstuffs making them unsafe to animals. The multi-toxin profiles in 58 commercial poultry feed samples collected from 19 districts in 17 states of Nigeria were determined by LC/ESI–MS/MS with a single extraction step and no clean-up. Sixtythree (56 fungal and seven bacterial) metabolites were detected with concentrations ranging up to 10,200 mgkg-1 in the case of aurofusarin. Fusarium toxins were the most prevalent group of fungal metabolites, whereas valinomycin occurred in more than 50% of the samples. Twelve non-regulatory fungal and seven bacterial metabolites detected and quantified in this study have never been reported previously in naturally contaminated stored grains or finished feed. Among the regulatory toxins in poultry feed, aflatoxin concentrations in 62% of samples were above 20 mgkg 1, demonstrating high prevalence of unsafe levels of aflatoxins in Nigeria. Deoxynivalenol concentrations exceeded 1000 mgkg 1 in 10.3% of samples. Actions are required to reduce the consequences from regulatory mycotoxins and understand the risks of the single or co-occurrence of nonregulatory metabolites for the benefit of the poultry industry