UHPLC–MS quantification of coumarin and chlorogenic acid in extracts of the medicinal plants known as guaco (Mikania glomerata and Mikania laevigata)

In Brazil, Mikania glomerata Spreng. and M. laevigata Sch. Bip. ex Baker, Asteraceae, known popularly as guaco, are widely used for colds and asthma. Although coumarin is adopted as the chemical marker of both species, it was not always detected in M. glomerata, for which chlorogenic acid was identified and quantified instead. The purpose of this study was to develop and validate a method to quantify both coumarin and chlorogenic acid and apply it to extracts of plants identified as M. glomerata, M. laevigata, or as guaco, to determine the pattern of composition of these two species and to observe differences between oven-dried and lyophilized leaves. A method using ultra-high resolution liquid chromatography–mass spectrometry (UHPLC–MS) in the full scan mode was validated for selectivity, matrix effect, linearity, limits of detection and quantification, precision and accuracy. The concentration of coumarin varied between species and samples, therefore these two species should not be used interchangeably. The concentration of chlorogenic acid was also determined for all samples. The UHPLC–MS method permitted the quantification of coumarin and chlorogenic acid in 16 samples of guaco and several commercial samples were possibly misidentified. Keywords: Mikania glomerata, Mikania laevigata, Coumarin, Chlorogenic acid, UHPLC–MS analysi

Search for associations containing young stars (SACY): VIII. An updated census of spectroscopic binary systems exhibiting hints of non-universal multiplicity among their associations

peer reviewedContext. Nearby young associations offer one of the best opportunities for a detailed study of the properties of young stellar and substellar objects thanks to their proximity (<200 pc) and age (∼5-150 Myr). Previous works have identified spectroscopic (<5 au) binaries, close (5-1000 au) visual binaries, and wide or extremely wide (1000-100 000 au) binaries in the young associations. In most of the previous analyses, single-lined spectroscopic binaries (SB1) were identified based on radial velocities variations. However, this apparent variation may also be caused by mechanisms unrelated to multiplicity. Aims. We seek to update the spectroscopy binary fraction of the Search for Associations Containing Young stars (SACY) sample, taking into consideration all possible biases in our identification of binary candidates, such as activity and rotation. Methods. Using high-resolution spectroscopic observations, we produced ∼1300 cross-correlation functions (CCFs) to disentangle the previously mentioned sources of contamination. The radial velocity values we obtained were cross-matched with the literature and then used to revise and update the spectroscopic binary (SB) fraction in each object of the SACY association. In order to better describe the CCF profile, we calculated a set of high-order cross-correlation features to determine the origin of the variations in radial velocities. Results. We identified 68 SB candidates from our sample of 410 objects. Our results hint that at the possibility that the youngest associations have a higher SB fraction. Specifically, we found sensitivity-corrected SB fractions of 22-11+15% for Ïμ Cha, 31-14+16% for TW Hya and 32-8+9% for β Pictoris, in contrast to the five oldest associations we have sampled (∼35-125 Myr) which are ∼10% or lower. This result seems independent of the methodology used to asses membership to the associations. Conclusions. The new CCF analysis, radial velocity estimates, and SB candidates are particularly relevant for membership revision of targets in young stellar associations. These targets would be ideal candidates for follow-up campaigns using high-resolution techniques to confirm binarity, resolve orbits, and, ideally, calculate dynamical masses. Additionally, if the results on the SB fraction in the youngest associations were confirmed, it could hint at a non-universal multiplicity among SACY associations