1,943 research outputs found
An Investigation of the Effect of Tip Shape on the Low-speed Aerodynamic Characteristics of Large-scale Swept Wings
Novel approach for deriving genome wide SNP analysis data from archived blood spots.
RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.BACKGROUND: The ability to transport and store DNA at room temperature in low volumes has the advantage of optimising cost, time and storage space. Blood spots on adapted filter papers are popular for this, with FTA (Flinders Technology Associates) Whatman™TM technology being one of the most recent. Plant material, plasmids, viral particles, bacteria and animal blood have been stored and transported successfully using this technology, however the method of porcine DNA extraction from FTA Whatman™TM cards is a relatively new approach, allowing nucleic acids to be ready for downstream applications such as PCR, whole genome amplification, sequencing and subsequent application to single nucleotide polymorphism microarrays has hitherto been under-explored. FINDINGS: DNA was extracted from FTA Whatman™TM cards (following adaptations of the manufacturer's instructions), whole genome amplified and subsequently analysed to validate the integrity of the DNA for downstream SNP analysis. DNA was successfully extracted from 288/288 samples and amplified by WGA. Allele dropout post WGA, was observed in less than 2% of samples and there was no clear evidence of amplification bias nor contamination. Acceptable call rates on porcine SNP chips were also achieved using DNA extracted and amplified in this way. CONCLUSIONS: DNA extracted from FTA Whatman cards is of a high enough quality and quantity following whole genomic amplification to perform meaningful SNP chip studies
Use of a field-based water elutriation system for monitoring the in situ particle size characteristics of fluvial suspended sediment
Effective particle size characteristics of fluvial suspended sediment transported by lowland British rivers
Composite suspended sediment particles in river systems: Their incidence, dynamics and physical characteristics
Aerodynamic Study of a Wing-fuselage Combination Employing a Wing Swept Back 63 Degrees : Investigation of a Large-scale Model at Low Speed
Senior Recital:Diana K. Harris, Soprano Robert C. Walling, Tenor
Centennial Lecture Hall April 2, 1967 3:00p.m
Some sampling considerations in the design of effective strategies for monitoring sediment-associated transport
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