The impact of alkyl chain purity on lipid based nucleic acid delivery systems – is the utilization of lipid components with technical grade justified?

The physicochemical properties and transfection efficacies of two samples of a cationic lipid have been investigated and compared in 2D (monolayers at the air/liquid interface) and 3D (aqueous bulk dispersions) model systems using different techniques. The samples differ only in their chain composition due to the purity of the oleylamine (chain precursor). Lipid 8 (using the oleylamine of technical grade for cost-efficient synthesis) shows lateral phase separation in the Langmuir layers. However, the amount of attached DNA, determined by IRRAS, is for both samples the same. In 3D systems, lipid 8 p forms cubic phases, which disappear after addition of DNA. At physiological temperatures, both lipids (alone and in mixture with cholesterol) assemble to lamellar aggregates and exhibit comparable DNA delivery efficiency. This study demonstrates that non-lamellar structures are not compulsory for high transfection rates. The results legitimate the utilization of oleyl chains of technical grade in the synthesis of cationic transfection lipid

Thermal conductance of thin film YIG determined using Bayesian statistics

Thin film YIG (Y$_3$Fe$_5$O$_{12}$) is a prototypical material for experiments on thermally generated pure spin currents and the spin Seebeck effect. The 3-omega method is an established technique to measure the cross-plane thermal conductance of thin films, but can not be used in YIG/GGG (Ga$_3$Gd$_5$O$_{12}$) systems in its standard form. We use two-dimensional modeling of heat transport and introduce a technique based on Bayesian statistics to evaluate measurement data taken from the 3-omega method. Our analysis method allows us to study materials systems that have not been accessible with the conventionally used 3-omega analysis. Temperature dependent thermal conductance data of thin film YIG are of major importance for experiments in the field of spin-caloritronics. Here we show data between room temperature and 10 K for films covering a wide thickness range as well as the magnetic field effect on the thermal conductance between 10 K and 50 K

Observation of ferromagnetic resonance in strontium ruthenate (SrRuO3)

We report the observation of ferromagnetic resonance (FMR) in SrRuO3 using the time-resolved magneto-optical Kerr effect. The FMR oscillations in the time-domain appear in response to a sudden, optically induced change in the direction of easy-axis anistropy. The high FMR frequency, 250 GHz, and large Gilbert damping parameter, alpha ~ 1, are consistent with strong spin-orbit coupling. We find that the parameters associated with the magnetization dynamics, including alpha, have a non-monotonic temperature dependence, suggestive of a link to the anomalous Hall effect.Comment: submitted to Phys. Rev. Let

Determination of the spin-flip time in ferromagnetic SrRuO3 from time-resolved Kerr measurements

We report time-resolved Kerr effect measurements of magnetization dynamics in ferromagnetic SrRuO3. We observe that the demagnetization time slows substantially at temperatures within 15K of the Curie temperature, which is ~ 150K. We analyze the data with a phenomenological model that relates the demagnetization time to the spin flip time. In agreement with our observations the model yields a demagnetization time that is inversely proportional to T-Tc. We also make a direct comparison of the spin flip rate and the Gilbert damping coefficient showing that their ratio very close to kBTc, indicating a common origin for these phenomena

Draft Genome Sequence of the Caffeine-Degrading Methylotroph Methylorubrum populi Pinkel.

A pink-pigmented facultative methylotroph, Methylorubrum populi Pinkel, was isolated from compost by selective enrichment with caffeine (3,5,7-trimethylxanthine) as the sole carbon, nitrogen, and energy source. We report here its high-quality draft genome sequence, assembled in 35 contigs totaling 5,630,907 bp. We identified 5,681 protein-coding sequences, including those putatively involved in caffeine degradation

Multi-person Spatial Interaction in a Large Immersive Display Using Smartphones as Touchpads

In this paper, we present a multi-user interaction interface for a large immersive space that supports simultaneous screen interactions by combining (1) user input via personal smartphones and Bluetooth microphones, (2) spatial tracking via an overhead array of Kinect sensors, and (3) WebSocket interfaces to a webpage running on the large screen. Users are automatically, dynamically assigned personal and shared screen sub-spaces based on their tracked location with respect to the screen, and use a webpage on their personal smartphone for touchpad-type input. We report user experiments using our interaction framework that involve image selection and placement tasks, with the ultimate goal of realizing display-wall environments as viable, interactive workspaces with natural multimodal interfaces.Comment: 8 pages with reference

Are You Tampering With My Data?

We propose a novel approach towards adversarial attacks on neural networks (NN), focusing on tampering the data used for training instead of generating attacks on trained models. Our network-agnostic method creates a backdoor during training which can be exploited at test time to force a neural network to exhibit abnormal behaviour. We demonstrate on two widely used datasets (CIFAR-10 and SVHN) that a universal modification of just one pixel per image for all the images of a class in the training set is enough to corrupt the training procedure of several state-of-the-art deep neural networks causing the networks to misclassify any images to which the modification is applied. Our aim is to bring to the attention of the machine learning community, the possibility that even learning-based methods that are personally trained on public datasets can be subject to attacks by a skillful adversary.Comment: 18 page