Technical note: Feasibility of near infrared transmittance spectroscopy to predict cheese ripeness

The aim of the study was to evaluate the feasibility of near infrared (NIR) transmittance spectroscopy to predict cheese ripeness using the ratio of water-soluble nitrogen (WSN) to total nitrogen (TN) as an index of cheese maturity (WSN/TN). Fifty-two Protected Designation of Origin cow milk cheeses of 5 varieties (Asiago, Grana Padano, Montasio, Parmigiano Reggiano, and Piave) and different ripening times were available for laboratory and chemometric analyses. Reference measures of WSN and TN were matched with cheese spectral information obtained from ground samples by a NIR instrument that operated in transmittance mode for wavelengths from 850 to 1,050 nm. Prediction equations for WSN and TN were developed using (1) cross-validation on the whole data set and (2) external validation on a subset of the entire data. The WSN/TN was calculated as ratio of predicted WSN to predicted TN in cross-validation. The coefficients of determination for WSN and TN were >0.85 both in cross- and external validation. The high accuracy of the prediction equations for WSN and TN could facilitate implementation of NIR transmittance spectroscopy in the dairy industry to objectively, rapidly, and accurately monitor the ripeness of cheese through WSN/TN

Comparison of organic and conventional Italian cheeses chemical composition from parallel production

ABSTRACT: Although there are several studies comparing organic and conventional milk characteristics, very few focused on dairy processed products such as cheese. Thus, this study aimed for a detailed controlled examination of gross composition, minerals, and the fatty acid profile of organic (ORG) and conventional (CON) Italian cheeses from parallel production. Four Italian cheese types were analyzed: Latteria (ORG, n = 9; CON, n = 10); Asiago Protected Designation of Origin (PDO) fresco (ORG, n = 9; CON, n = 9); Caciotta (ORG, n = 8; CON, n = 8); and Mozzarella Traditional Specialty Guaranteed (TSG; ORG, n = 14; CON, n = 14). Cheese samples were collected from September 2020 to August 2021. Gross composition, minerals, and fatty acids were determined using infrared spectroscopy. Within each cheese type, paired ORG and CON samples were compared using a nonparametric Wilcoxon signed-rank test. Latteria showed lower PUFA, n-3, and n-6 content, and greater Fe, K, C10:0, C12:0, and C16:0 content in ORG than in CON. Asiago PDO fresco showed lower protein and Zn content and greater salt, ash, and Na content in ORG than in CON. Caciotta showed lower ash, n-3, and n-6 content and greater K, C4:0, C8:0, C10:0, C14:0, and C16:0 content in ORG than in CON. Mozzarella TSG showed lower fat and, therefore, fatty acid content, and greater moisture, ash, and Mg content in ORG than in CON. In conclusion, few significant differences in chemical composition were observed between ORG and CON cheeses, regardless of the type considered. Moreover, Asiago PDO fresco showed fewer significant differences between ORG and CON compared with Latteria, Caciotta, and Mozzarella TSG

Short communication: Fourier-transform mid-infrared spectroscopy to predict coagulation and acidity traits of sheep bulk milk.

Sheep milk is mainly transformed into cheese; thus, the dairy industry seeks more rapid and cost-effective methods of analysis to determine milk coagulation and acidity traits. This study aimed to assess the feasibility of Fourier-transform mid-infrared spectroscopy to determine milk coagulation and acidity traits of sheep bulk milk and to classify milk samples according to their renneting capacity. A total of 465 bulk milk samples collected in 140 single-breed flocks of Comisana (84 samples, 24 flocks) and Sarda (381 samples, 116 flocks) breeds located in Central Italy were analyzed for coagulation properties (rennet coagulation time, curd firming time, and curd firmness) and acidity traits (pH and titratable acidity) using standard laboratory procedures. Fourier-transform mid-infrared spectroscopy prediction models for these traits were built using partial least squares regression analysis and were externally validated by randomly dividing the full data set into a calibration set (75%) and a validation set (25%). The discriminant capacity of the rennet coagulation time prediction model was determined using partial least squares discriminant analysis. Prediction models were more accurate for acidity traits than for milk coagulation properties, and the ratio of prediction to deviation ranged from 1.01 (curd firmness) to 2.14 (pH). Moreover, the discriminant analysis led to an overall accuracy of 74 and 66% for the calibration and validation sets, respectively, with greater sensitivity for samples that coagulated between 10 and 20 min and greater specificity to detect early-coagulating (<10 min) and late-coagulating (20-30 min) samples. Results suggest that Fourier-transform mid-infrared spectroscopy has the potential to help the dairy sheep industry identify milk with better coagulation ability for cheese production and thus improve milk transformation efficiency. However, further research is needed before this information can be exploited at the industry level

Phenotypic analysis of milk coagulation properties and mineral content of Pinzgauer cattle breed

Abstract. This study aimed to characterize milk coagulation properties (rennet coagulation time, curd-firming time and curd firmness 30min after rennet addition to milk) and major mineral contents (Ca, Mg, P, K and Na) in Pinzgauer dual-purpose cattle breed. The edited dataset consisted of 7763 milk observations from 851 cows reared in 60 herds in the Alpine area of Bolzano province (Italy). Data were analysed through a linear mixed model which included stage of lactation, parity and their interaction as fixed effects, and cow and herd test date as random effects. Rennet coagulation time, curd-firming time and curd firmness 30min after rennet addition to milk averaged 22.66min, 5.53min and 16.79mm, respectively. The most abundant minerals were P (1495mgkg−1) and Ca (1344mgkg−1), and the least abundant Mg (141mgkg−1). Compared to their older contemporaries, early-lactating younger animals yielded milk that was more favourable for cheese production (i.e. with shorter coagulation time and stronger curd firmness). Mineral contents were lower in milk of primiparous than multiparous cows, except for Na. Moreover, Ca, Mg, P and Na contents decreased from parturition to peak of lactation and increased thereafter, except for K, which exhibited an opposite trend. Our results showed that Pinzgauer breed produced milk with better coagulation properties and mineral content, from a technological point of view, in first than later parities and in early than late lactation. The characterization of milk coagulation properties and mineral content in autochthonous breeds is important to increase their value and marketability of their products.</p

Short communication: Prediction of milk coagulation and acidity traits in Mediterranean buffalo milk using Fourier-transform mid-infrared spectroscopy

Milk coagulation and acidity traits are important factors to inform the cheesemaking process. Those traits have been deeply studied in bovine milk, whereas scarce information is available for buffalo milk. However, the dairy industry is interested in a method to determine milk coagulation and acidity features quickly and in a cost-effective manner, which could be provided by Fourier-transform mid-infrared (FT-MIR) spectroscopy. The aim of this study was to evaluate the potential of FT-MIR to predict coagulation and acidity traits of Mediterranean buffalo milk. A total of 654 records from 36 herds located in central Italy with information on milk yield, somatic cell score, milk chemical composition, milk acidity [pH, titratable acidity (TA)], and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness) were available for statistical analysis. Reference measures of milk acidity and coagulation properties were matched with milk spectral information, and FT-MIR prediction models were built using partial least squares regression. The data set was divided into a calibration set (75%) and a validation set (25%). The capacity of FT-MIR spectroscopy to correctly classify milk samples based on their renneting ability was evaluated by a canonical discriminant analysis. Average values for milk coagulation traits were 13.32 min, 3.24 min, and 39.27 mm for rennet coagulation time, curd firming time, and curd firmness, respectively. Milk acidity traits averaged 6.66 (pH) and 7.22 Soxhlet-Henkel degrees/100 mL (TA). All milk coagulation and acidity traits, except for pH, had high variability (17 to 46%). Prediction models of coagulation traits were moderately to scarcely accurate, whereas the coefficients of determination of external validation were 0.76 and 0.66 for pH and TA, respectively. Canonical discriminant analysis indicated that information on milk coagulating ability is present in the MIR spectra, and the model correctly classified as noncoagulating the 91.57 and 67.86% of milk samples in the calibration and validation sets, respectively. In conclusion, our results can be relevant to the dairy industry to classify buffalo milk samples before processing

A short-term comparison of wheat straw and poplar wood chips used as litter in tiestalls on hygiene, milk, and behavior of lactating dairy cows

A short-term study was conducted to compare the effect of using poplar wood chips (PWC) instead of wheat straw (WS) litter in dairy cows. A total of 38 lactating Holstein cows (204 ± 119 days in milk, 26.9 ± 6.5 kg of milk yield [MY]) were housed in a tiestall farm for a 10-d trial including 5 d of adaptation followed by 5 sampling days (from d 5 to 10). Cows were divided into 2 homogeneous groups: one group was bedded with WS, and the second with PWC. Both litter materials were provided in the amount of 7 kg/stall per d. Each group was composed of 3 subgroups of 6 or 7 cows; the subgroups were physically separated along the feeding line by wooden boards. During the sampling days, fecal composition, used litter composition, and bacterial count (Clostridium spp., Salmonella spp., Escherichia coli, Lactobacillus, and total bacterial count) were analyzed by subgroup twice a day. On d 1 and from d 5 to 10, udder hygiene score and cow cleanliness score were also evaluated individually twice a day. Meanwhile MY, milk hygiene (total bacterial count [TBC], coliform bacterial count [CBC], and spore-forming unit [SFU]) and quality were measured and analyzed from 9 animals per group. Moreover, individual animal behavior (body position and behavioral traits) and subgroup dry matter intake were measured on d 9 and 10. Fecal dry matter did not differ between groups, PWC had the lowest used litter moisture and N content favoring the highest clean cow frequency, but also gave rise to the greatest used litter microbial contamination. The MY, milk quality, TBC, SFU, and CBC were similar. The lying behavior frequency was similar between groups. However, the PWC group showed the lowest sleeping frequency, the highest frequency of other behaviors (including discomfort signs), and the lowest dry matter intake. However, despite this apparent reduction in cow comfort, no biologically important differences were observed in this short-term study between cows on PWC and WS in milk production or hygiene

Invited review: Iodine level in dairy products—A feed-to-fork overview

The theme of iodine in the dairy sector is of particular interest due to the involvement and the interconnection of several stakeholders along the dairy food chain. Iodine plays a fundamental role in animal nutrition and physiology, and in cattle it is an essential micronutrient during lactation and for fetal development and the calf's growth. Its correct use in food supplementation is crucial to guarantee the animal's recommended daily requirement to avoid excess intake and long-term toxicity. Milk iodine is fundamental for public health, being one of the major sources of iodine in Mediterranean and Western diets. Public authorities and the scientific community have made great efforts to address how and to what extent different drivers may affect milk iodine concentration. The scientific literature concurs that the amount of iodine administered through animal feed and mineral supplements is the most important factor affecting its concentration in milk of most common dairy species. Additionally, farming practices related to milking (e.g., use of iodized teat sanitizers), herd management (e.g., pasture vs. confinement), and other environmental factors (e.g., seasonality) have been identified as sources of variation of milk iodine concentration. Overall, the aim of this review is to provide a multilevel overview on the mechanisms that contribute to the iodine concentration of milk and dairy products

Creación y persistencia de la aversión condicionada a hojas de vid en ovino. Aplicación al pastoreo de viñedos en primavera

El pastoreo en viñedos se ve limitado por el daño que las ovejas pueden provocar en el cultivo, dada la alta palatabilidad de las hojas de vid. El objetivo del presente trabajo fue crear aversión a hojas de vid y evaluar su persistencia en ovejas (Manchega, n = 12; Lacaune, n = 12) durante tres años. El estudio incluyó la creación y validación de la aversión en condiciones experimentales en el primer año y en pastoreo en viñedos comerciales los años dosytres. Lostratamientos consistieron en ovejas avertidas (AV, 225 mg LiCl/kg PV) y ovejas control (C, tratadas con agua). Durante el año uno la aversión se validó (30 min/prueba) en el aprisco y en viña simulada. Los siguientes años, las ovejas AV pastaron dos viñedos comerciales con cubierta espontánea (viñedo A y B). El tratamiento con LiCl dio lugar a que las ovejas AV rechazaran consumir las hojas de vid (AV vs. C; 0 vs. 95 ± 5 g/d). En los viñedos comerciales, las ovejas AV redujeron la materia seca de la cubierta vegetal en un 68 ± 8% y 44 ± 4% para A y B respectivamente. Sin embargo, cuando la cubierta fue poco palatable y escasa, las ovejas AV empezaron a consumir las hojas y brotes de vid,siendo necesaria una nueva dosis de LiCl (A, 100%; B, 50% ovejas). En conclusión, la AV fue una herramienta efectiva para controlar la cubierta vegetal del viñedo en primavera,siendo necesaria una supervisión de los animales

Complement in glomerular injury

In recent years, research into the role of complement in the immunopathogenesis of renal disease has broadened our understanding of the fragile balance between the protective and harmful functions of the complement system. Interventions into the complement system in various models of immune-mediated renal disease have resulted in both favourable and unfavourable effects and will allow us to precisely define the level of the complement cascade at which a therapeutic intervention will result in an optimal effect. The discovery of mutations of complement regulatory molecules has established a role of complement in the haemolytic uremic syndrome and membranoproliferative glomerulonephritis, and genotyping for mutations of the complement system are already leaving the research laboratory and have entered clinical practice. These clinical discoveries have resulted in the creation of relevant animal models which may provide crucial information for the development of highly specific therapeutic agents. Research into the role of complement in proteinuria has helped to understand pathways of inflammation which ultimately lead to renal failure irrespective of the underlying renal disease and is of major importance for the majority of renal patients. Complement science is a highly exciting area of translational research and hopefully will result in meaningful therapeutic advances in the near future

aHUS caused by complement dysregulation: new therapies on the horizon

Atypical hemolytic uremic syndrome (aHUS) is a heterogeneous disease that is caused by defective complement regulation in over 50% of cases. Mutations have been identified in genes encoding both complement regulators [complement factor H (CFH), complement factor I (CFI), complement factor H-related proteins (CFHR), and membrane cofactor protein (MCP)], as well as complement activators [complement factor B (CFB) and C3]. More recently, mutations have also been identified in thrombomodulin (THBD), an anticoagulant glycoprotein that plays a role in the inactivation of C3a and C5a. Inhibitory autoantibodies to CFH account for an additional 5–10% of cases and can occur in isolation or in association with mutations in CFH, CFI, CFHR 1, 3, 4, and MCP. Plasma therapies are considered the mainstay of therapy in aHUS secondary to defective complement regulation and may be administered as plasma infusions or plasma exchange. However, in certain cases, despite initiation of plasma therapy, renal function continues to deteriorate with progression to end-stage renal disease and renal transplantation. Recently, eculizumab, a humanized monoclonal antibody against C5, has been described as an effective therapeutic strategy in the management of refractory aHUS that has failed to respond to plasma therapy. Clinical trials are now underway to further evaluate the efficacy of eculizumab in the management of both plasma-sensitive and plasma-resistant aHUS