Antimicrobial peptides: agents of border protection for companion animals.

Over the past 20 years, there have been significant inroads into understanding the roles of antimicrobial peptides in homeostatic functions and their involvement in disease pathogenesis. In addition to direct antimicrobial activity, these peptides participate in many cellular functions, including chemotaxis, wound healing and even determination of canine coat colour. Various biological and genetic approaches have helped to elucidate the role of antimicrobial peptides with respect to innate immunity and host defense. Associations of antimicrobial peptides with various skin diseases, including psoriasis, rosacea and atopic dermatitis, have been documented in humans. In the longer term, therapeutic modulation of antimicrobial peptide expression may provide effective new treatments for disease. This review highlights current knowledge about antimicrobial peptides of the skin and circulating leukocytes, with particular focus on relevance to physiology and disease in companion animals

Diminished conditioned responding to the nicotine stimulus by antidepressant drugs with differing specificity for the serotonin and norepinephrine transporter

People diagnosed with depression also tend to have a co-morbid nicotine addiction. Thus, there is interest in whether medications used to treat depression alter the effects of nicotine. This study assessed whether the antidepressant drugs citalopram, imipramine, and reboxetine, with differing specificity for the serotonin and norepinephrine transporter, altered responding controlled by the conditional stimulus (CS) effects of nicotine. Rats received intermixed 20-min nicotine (0.4 mg base/kg, SC) and saline sessions. On nicotine sessions, rats had intermittent access to sucrose; no sucrose was available on saline sessions. After discrimination performance stabilized and a nicotine generalization curve (0.025–0.4 mg/kg) was established, the antidepressant drugs were assessed. In these tests, rats were pretreated with citalopram (1–17 mg/kg), imipramine (1–17 mg/ kg), or reboxetine (1–30 mg/kg) before the training dose of nicotine and placement in a chamber for a 4-min extinction test. At the higher doses, all three antidepressant drugs blocked responding evoked by the nicotine CS and decreased nicotine-induced hyperactivity. When these higher doses of citalopram, imipramine, and reboxetine were tested alone (no nicotine), they decreased chamber activity and/or dipper entries. Nevertheless, all three drugs produced partial or complete blockade of the CS effects of nicotine at doses that produced no effect on dipper entries or chamber entries. This finding suggests that both neurotransmitters play a role in the CS effects of nicotine and that modifications in these systems by antidepressants may be clinically relevant

Developing proactive communication strategies for a potential pandemic

Communication through the Australian media during a potential avian influenza epidemic could act to inform the public OR misinform, contributing to unnecessary public panic and undesirable responses. This project is part of ongoing research to assess Australians’ knowledge and perceptions of bird flu which will allow the development of public service advertising messages for use by the Australian government in the event of a bird flu outbreak or pandemic. Focus group and CATI survey results on bird flu perceptions were used by an Australian advertising agency to prepare two campaign concepts. The concepts (story boards and print ads) were tested using focus groups by two independent market research companies in July 2006. This paper reports on the results of the ad-testing, and provides recommendations for the development and refinement of a public communication campaign to minimise public panic during a bird flu outbreak or pandemic in Australia

The Effect of Oral Contraceptive Pill Use on Knee Joint Laxity in Women

Schultz et al. (2004) demonstrated that 63% of the change in knee joint laxity (KJL) during a menstrual cycle was due to associated fluctuations in sex hormone levels. Use of oral contraceptives modulates the fluctuation of sex hormone levels during the menstrual cycle and thus may reduce fluctuation in KJL PURPOSE: Given that increased KJL is a known risk factor for sustaining knee injuries, the purpose of this study was to examine the effect of oral contraceptive use on KJL during the follicular, ovulation, and luteal phases of the menstrual cycle. METHODS: Sixty college-age women were screened for participation and fourteen (20.07±1.21 years, 163.05± 9.70, and 66.81± 12.32 kg) met the inclusion criteria, provided informed consent, and participated in the study. Based on screening questionnaires, participants were sorted into groups, oral contraceptive users (OC) and non-users (NOC). Each participant’s KJL was measured on six occasions, five days apart. KJL was measured using a KT-1000 Knee Arthrometer at 133 N. Measurements on days 1-7, 11-14, and 19-22 were used for data analysis to correspond with the three phases of the menstrual cycle. A 2x3 (group x phase) mixed model ANOVA was used to compare KJL between groups and across the three phases of the menstrual cycle. RESULTS: KJL are reported in Table 1. Group and phase did not interact to affect KJL (F(2,24)=1.92, p=0.17). KJL did not differ between OC and NOC users across the menstrual cycle (F(1,12)=0.07, p=0.80), and was not different between any phase of the menstrual cycle (F(2,24)=0.14, p=0.87). However, given that the spike in estradiol associated with the ovulation phase has been suggested to affect ligament laxity, a comparison between groups during this phase was conducted. Though KJL was larger for NOC than OC, the results of a one-tailed independent t-test suggest that this difference was not statistically significantly (t(12)= 1.72, p = 0.06). However, this difference was characterized by a large effect size (Cohen d= 0.92) suggesting that NOC users experience more KJL during the ovulation phase than OC users. CONCLUSION: The results of the study indicate that OC may play a role in KJL. However, with limited statistical power of these analyses, additional data are needed to fully assess this effect

Nicotine, Tobacco Use, and the 55th Nebraska Symposium on Motivation

Tobacco use is a worldwide health problem. As so well stated by Mackay and Ericksen (2002), “No other consumer product is as dangerous, or kills as many people. Tobacco kills more than AIDS, legal drugs, illegal drugs, road accidents, murder, and suicide combined” (p. 36). Imagine the lives saved, and the amount of pain, emotional suffering, and fiscal burden alleviated, if we could devise approaches that helped current tobacco users quit and remain abstinent, and prevented new smokers from emerging. Although these idealistic goals are worth pursuing, improving cessation rates by only a small fraction, or making small gains in preventing people from experimenting with tobacco, would nevertheless translate into significant improvement in the health and well-being of countless thousands worldwide as well as financial savings to employers, government institutions, and the heath care system. Even such small, incremental steps require a concerted and coordinated effort by basic scientists, clinical researchers and practitioners, and policy makers to discover the basis of tobacco dependence and apply that knowledge to the implementation of prevention policies and smoking cessation aids. This year\u27s Nebraska Symposium on Motivation was devoted to research on the drug that is widely believed to form the basis of tobacco use and dependence, nicotine

Diminished conditioned responding to the nicotine stimulus by antidepressant drugs with differing specificity for the serotonin and norepinephrine transporter

People diagnosed with depression also tend to have a co-morbid nicotine addiction. Thus, there is interest in whether medications used to treat depression alter the effects of nicotine. This study assessed whether the antidepressant drugs citalopram, imipramine, and reboxetine, with differing specificity for the serotonin and norepinephrine transporter, altered responding controlled by the conditional stimulus (CS) effects of nicotine. Rats received intermixed 20-min nicotine (0.4 mg base/kg, SC) and saline sessions. On nicotine sessions, rats had intermittent access to sucrose; no sucrose was available on saline sessions. After discrimination performance stabilized and a nicotine generalization curve (0.025–0.4 mg/kg) was established, the antidepressant drugs were assessed. In these tests, rats were pretreated with citalopram (1–17 mg/kg), imipramine (1–17 mg/ kg), or reboxetine (1–30 mg/kg) before the training dose of nicotine and placement in a chamber for a 4-min extinction test. At the higher doses, all three antidepressant drugs blocked responding evoked by the nicotine CS and decreased nicotine-induced hyperactivity. When these higher doses of citalopram, imipramine, and reboxetine were tested alone (no nicotine), they decreased chamber activity and/or dipper entries. Nevertheless, all three drugs produced partial or complete blockade of the CS effects of nicotine at doses that produced no effect on dipper entries or chamber entries. This finding suggests that both neurotransmitters play a role in the CS effects of nicotine and that modifications in these systems by antidepressants may be clinically relevant

Extensive variation in the intelectin gene family in laboratory and wild mouse strains

Intelectins are a family of multimeric secreted proteins that bind microbe-specific glycans. Both genetic and functional studies have suggested that intelectins have an important role in innate immunity and are involved in the etiology of various human diseases, including inflammatory bowel disease. Experiments investigating the role of intelectins in human disease using mouse models are limited by the fact that there is not a clear one-to-one relationship between intelectin genes in humans and mice, and that the number of intelectin genes varies between different mouse strains. In this study we show by gene sequence and gene expression analysis that human intelectin-1 (ITLN1) has multiple orthologues in mice, including a functional homologue Itln1; however, human intelectin-2 has no such orthologue or homologue. We confirm that all sub-strains of the C57 mouse strain have a large deletion resulting in retention of only one intelectin gene, Itln1. The majority of laboratory strains have a full complement of six intelectin genes, except CAST, SPRET, SKIVE, MOLF and PANCEVO strains, which are derived from different mouse species/subspecies and encode different complements of intelectin genes. In wild mice, intelectin deletions are polymorphic in Mus musculus castaneus and Mus musculus domesticus. Further sequence analysis shows that Itln3 and Itln5 are polymorphic pseudogenes due to premature truncating mutations, and that mouse Itln1 has undergone recent adaptive evolution. Taken together, our study shows extensive diversity in intelectin genes in both laboratory and wild-mice, suggesting a pattern of birth-and-death evolution. In addition, our data provide a foundation for further experimental investigation of the role of intelectins in disease

Collaboration between academics and teachers : a complex relationship

Collaboration between academics and teachers has become increasingly prevalent over recent years. Whether its aim is joint research or continuing professional development for teachers, collaboration seems to offer a realistic opportunity for reducing the perceived gap between theory and practice. However, collaboration is not merely academics and teachers working together on a common project. It is complex in nature and involves a range of requirements that must be satisfied in order to maximise the potential of the relationship. In this paper we will theorise on the nature of academics and teachers working together and suggest that a working relationship between academic researchers and teachers can be one of three models: client–supplier, a coercive relationship or a collaborative relationship. We identify and unpack specific factors that underpin collaboration and suggest a number of concrete actions to establish collaboration between academics and teachers. We draw heavily from existing literature and our own reflections on two collaborative projects with which we have recently been involved. We use data from these projects to provide a number of anecdotes from the teachers who participated to support our own reflections. Finally, we suggest that further research should investigate the different ways attempts to collaborate fail, to build a more complete sense of the problems and potential of this special relationship. Keywords: collaboration; continuing professional development; action research; science teacher