Sanitizing the fortress: protection of ant brood and nest material by worker antibiotics

Social groups are at particular risk for parasite infection, which is heightened in eusocial insects by the low genetic diversity of individuals within a colony. To combat this, adult ants have evolved a suite of defenses to protect each other, including the production of antimicrobial secretions. However, it is the brood in a colony that are most vulnerable to parasites because their individual defenses are limited, and the nest material in which ants live is also likely to be prone to colonization by potential parasites. Here, we investigate in two ant species whether adult workers use their antimicrobial secretions not only to protect each other but also to sanitize the vulnerable brood and nest material. We find that, in both leaf-cutting ants and weaver ants, the survival of the brood was reduced and the sporulation of parasitic fungi from them increased, when the workers nursing them lacked functional antimicrobial-producing glands. This was the case for both larvae that were experimentally treated with a fungal parasite (Metarhizium) and control larvae which developed infections of an opportunistic fungal parasite (Aspergillus). Similarly, fungi were more likely to grow on the nest material of both ant species if the glands of attending workers were blocked. The results show that the defense of brood and sanitization of nest material are important functions of the antimicrobial secretions of adult ants and that ubiquitous, opportunistic fungi may be a more important driver of the evolution of these defenses than rarer, specialist parasites

Detecting Delamination via Nonlinear Wave Scattering in a Bonded Elastic Bar

In this paper we examine the effect of delamination on wave scattering, with the aim of creating a control measure for layered waveguides of various bonding types. Previous works have considered specific widths of solitary waves for the simulations, without analysing the effect of changing the soliton parameters. We consider two multi-layered structures: one containing delamination "sandwiched" by perfect bonding and one containing delamination but "sandwiched" by soft bonding. These structures are modelled by coupled Boussinesq-type equations. Matched asymptotic multiple-scale expansions lead to coupled Ostrovsky equations in soft bonded regions and Korteweg-De Vries equations in the perfectly bonded and delaminated region. We use the Inverse Scattering Transform to predict the behaviour in the delaminated regions. In both cases, numerical analysis shows that we can predict the delamination length by changes in the wave structure, and that these changes depend upon the Full Width at Half Magnitude (FWHM) of the incident soliton. In the case of perfect bonding, we derive a theoretical prediction for the change and confirm this numerically. For the soft bonding case, we numerically identify a similar relationship using the change in amplitude. Therefore we only need to compute one curve to determine the behaviour for any incident solitary wave, creating a framework for designing measurement campaigns for rigorously testing the integrity of layered structures.Comment: 12 pages, 7 figure

Phosphatase activity and organic phosphorus turnover on a high Arctic glacier

Arctic glacier surfaces harbour abundant microbial communities consisting mainly of heterotrophic and photoautotrophic bacteria. The microbes must cope with low concentrations of nutrients and with the fact that both the dissolved and debris-bound nutrient pools are dominated by organic phases. Here we provide evidence that phosphorus (P) is deficient in the supraglacial environment on a Svalbard glacier, we quantify the enzymatic activity of phosphatases in the system and we estimate the contribution of the microbes to the cycling of the dominant organic P in the supraglacial environment. Incubation of cryoconite debris revealed significant phosphatase activity in the samples (19–67 nmol MUP g<sup>−1</sup> h<sup>−1</sup>). It was inhibited by inorganic P during incubations and had its optimum at around 30°C. The phosphatase activity measured at near-in situ temperature and substrate concentration suggests that the available dissolved organic P can be turned over by microbes within ~3–11 h on the glacier surface. By contrast, the amount of potentially bioavailable debris-bound organic P is sufficient for a whole ablation season. However, it is apparent that some of this potentially bioavailable debris-bound P is not accessible to the microbes

Factors affecting dairy farmers' attitudes towards antimicrobial medicine usage in cattle in England and Wales

There has been growing concern about bacterial resistance to antimicrobials in the farmed livestock sector. Attention has turned to sub-optimal use of antimicrobials as a driver of resistance. Recent reviews have identified a lack of data on the pattern of antimicrobial use as an impediment to the design of measures to tackle this growing problem. This paper reports on a study that explored use of antibiotics by dairy farmers and factors influencing their decision-making around this usage. We found that respondents had either recently reduced their use of antibiotics, or planned to do so. Advice from their veterinarian was instrumental in this. Over 70% thought reducing antibiotic usage would be a good thing to do. The most influential source of information used was their own veterinarian. Some 50% were unaware of the available guidelines on use in cattle production. However, 97% thought it important to keep treatment records. The Theory of Planned Behaviour was used to identify dairy farmers’ drivers and barriers to reduce use of antibiotics. Intention to reduce usage was weakly correlated with current and past practice of antibiotic use, whilst the strongest driver was respondents’ belief that their social and advisory network would approve of them doing this. The higher the proportion of income from milk production and the greater the chance of remaining in milk production, the significantly higher the likelihood of farmers exhibiting positive intention to reduce antibiotic usage. Such farmers may be more commercially minded than others and thus more cost-conscious or, perhaps, more aware of possible future restrictions. Strong correlation was found between farmers’ perception of their social referents’ beliefs and farmers’ intent to reduce antibiotic use. Policy makers should target these social referents, especially veterinarians, with information on the benefits from, and the means to, achieving reductions in antibiotic usage. Information on sub-optimal use of antibiotics as a driver of resistance in dairy herds and in humans along with advice on best farm practice to minimise risk of disease and ensure animal welfare, complemented with data on potential cost savings from reduced antibiotic use would help improve poor practice

Dark ice dynamics of the south-west Greenland Ice Sheet

Runoff from the Greenland Ice Sheet (GrIS) has increased in recent years due largely to changes in atmospheric circulation and atmospheric warming. Albedo reductions resulting from these changes have amplified surface melting. Some of the largest declines in GrIS albedo have occurred in the ablation zone of the south-west sector and are associated with the development of "dark" ice surfaces. Field observations at local scales reveal that a variety of light-absorbing impurities (LAIs) can be present on the surface, ranging from inorganic particulates to cryoconite materials and ice algae. Meanwhile, satellite observations show that the areal extent of dark ice has varied significantly between recent successive melt seasons. However, the processes that drive such large interannual variability in dark ice extent remain essentially unconstrained. At present we are therefore unable to project how the albedo of bare ice sectors of the GrIS will evolve in the future, causing uncertainty in the projected sea level contribution from the GrIS over the coming decades. Here we use MODIS satellite imagery to examine dark ice dynamics on the south-west GrIS each year from 2000 to 2016. We quantify dark ice in terms of its annual extent, duration, intensity and timing of first appearance. Not only does dark ice extent vary significantly between years but so too does its duration (from 0 to > 80 % of June-July-August, JJA), intensity and the timing of its first appearance. Comparison of dark ice dynamics with potential meteorological drivers from the regional climate model MAR reveals that the JJA sensible heat flux, the number of positive minimum-air-temperature days and the timing of bare ice appearance are significant interannual synoptic controls. We use these findings to identify the surface processes which are most likely to explain recent dark ice dynamics. We suggest that whilst the spatial distribution of dark ice is best explained by outcropping of particulates from ablating ice, these particulates alone do not drive dark ice dynamics. Instead, they may enable the growth of pigmented ice algal assemblages which cause visible surface darkening, but only when the climatological prerequisites of liquid meltwater presence and sufficient photosynthetically active radiation fluxes are met. Further field studies are required to fully constrain the processes by which ice algae growth proceeds and the apparent dependency of algae growth on melt-out particulates

Three-dimensional steep wave impact on a vertical cylinder

In the present study we investigate the 3-D hydrodynamic slamming problem on a vertical cylinder due to the impact of a steep wave that is moving with a steady velocity. The linear theory of the velocity potential is employed by assuming inviscid, incompressible fluid and irrotational flow. As the problem is set in 3-D space, the employment of the Wagner condition is essential. The set of equations we pose, is presented as a mixed boundary value problem for Laplace's equation in 3-D. Apart from the mixed-type of boundary conditions, the problem is complicated by considering that the region of wetted surface of the cylinder is a set whose boundary depends on the vertical coordinate on the cylinder up to the free-surface. We make some simple assumptions at the start but otherwise we proceed analytically. We find closed-form relations for the hydrodynamic variables, namely the time dependent potential, the pressure impulse, the shape of the wave front (from the contact point to beyond the cylinder) and the slamming force

Sustainability in Turbulent Times: Lessons from the Nexus Network for supporting transdisciplinary research

This is the final version. Available from the Nexus Network via the link in this recordEconomic and Social Research Council (ESRC

Effective Viscosity of a Dilute Suspension of Membrane-bound Inclusions

When particulate suspensions are sheared, perturbations in the shear flows around the rigid particles increase the local energy dissipation, so that the viscosity of the suspension is effectively higher than that of the solvent. For bulk (three-dimensional) fluids, understanding this viscosity enhancement is a classic problem in hydrodynamics that originated over a century ago with Einstein's study of a dilute suspension of spherical particles. \cite{Einstein1} In this paper, we investigate the analogous problem of the effective viscosity of a suspension of disks embedded in a two-dimensional membrane or interface. Unlike the hydrodynamics of bulk fluids, low-Reynolds number membrane hydrodynamics is characterized by an inherent length scale generated by the coupling of the membrane to the bulk fluids that surround it. As a result, we find that the size of the particles in the suspension relative to this hydrodynamic length scale has a dramatic effect on the effective viscosity of the suspension. Our study also helps to elucidate the mathematical tools needed to solve the mixed boundary value problems that generically arise when considering the motion of rigid inclusions in fluid membranes.Comment: 33 pages, 4 figures (preprint); submitted to Physics of Fluid