Ergosterol Effect on the Desaturation of 14C-Cis-Vaccenate in Tetrahymena

Supplement of ergosterol to the growth medium of the ciliated protozoan Tetrahymena pyriformis W leads to incorporation of the foreign sterol within cell membranes and suppression of synthesis of the native sterol-like compound tetrahymanol, as well as to changes in the fatty acid compositions of several major classes of membrane lipid. Alteration of fatty acid composition is thought to represent a regulatory mechanism whereby optimum membrane fluidity is maintained when the slightly dissimilar foreign sterol is added into the phospholipid bilayer of the membranes. The present study, using several different conditions of growth temperature, substrate concentrations and incubation time, and ergosterol concentrations and exposure time, is an attempt to provide evidence supporting a hypothetical regulatory mechanism. This mechanism proposes that there is a feedback regulation by membrane-bound sterol on an enzyme or enzymes involved in synthesis of the long chain fatty acids contained in membrane phospholipid. Such a mechanism could account for the balance between sterol and fatty acid content of membrane. The data presented here show that a statistically significant increase in desaturation of 14C-cis-vaccenate can be demonstrated in Tetrahymena cell cultures whose membranes contain the foreign sterol, when growth temperature is maintained at 20° or 29.5°. Tetrahymena desaturated 14C-cis-vaccenate substrate in both ergosterol supplemented and normal cultures. The 14C labeled product, 6,11-18:2 was recovered and separated by silver nitrate-Unisil column chromatography

Political candidates in infotainment programmes and their emotional effects on Twitter: An analysis of the 2015 Spanish general elections pre-campaign season

This is an Accepted Manuscript of an article published by Taylor & Francis in Contemporary Social Science on 2019, available online: http://www.tandfonline.com/10.1080/21582041.2017.1367833.[EN] The infotainment format offers candidates an informal setting to show a more personal side of themselves to the electorate, opening themselves up to potential voters. An example of media hybridisation, social networks users can immediately comment on infotainment television programmes, a process known as second screening. These second screeners tend to be especially active in politics. This paper analyses the immediate emotional reaction of these users as they watch infotainment programmes that air during the campaign or pre-campaign seasons and feature political candidates as guests. We have confirmed that second screeners react more emotionally towards the candidate when his or her party is mentioned, and less emotionally when the host displays an aggressive attitude through his or her non-verbal communication. When issues related to the candidate¿s personal lives are discussed, users¿ emotional reactions improve slightly. The relevance of this research stems from the fact that we are witnessing the consolidation of a politics that increasingly strays from ideological questions, and instead focuses on more emotional and personal issues.This work was supported by the Ministerio de Economia y Competitividad under Grants CSO2013-43960-R and CSO2016-77331-C2-1-R.Baviera, T.; Peris, À.; Cano-Orón, L. (2019). Political candidates in infotainment programmes and their emotional effects on Twitter: An analysis of the 2015 Spanish general elections pre-campaign season. 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The Hybrid Media System. doi:10.1093/acprof:oso/9780199759477.001.0001Dang-Xuan, L., Stieglitz, S., Wladarsch, J., & Neuberger, C. (2013). AN INVESTIGATION OF INFLUENTIALS AND THE ROLE OF SENTIMENT IN POLITICAL COMMUNICATION ON TWITTER DURING ELECTION PERIODS. Information, Communication & Society, 16(5), 795-825. doi:10.1080/1369118x.2013.783608Giglietto, F., & Selva, D. (2014). Second Screen and Participation: A Content Analysis on a Full Season Dataset of Tweets. Journal of Communication, 64(2), 260-277. doi:10.1111/jcom.12085Grabe, M. E., & Bucy, E. P. (2009). Image Bite Politics. doi:10.1093/acprof:oso/9780195372076.001.0001Guo, L., & Vargo, C. (2015). The Power of Message Networks: A Big-Data Analysis of the Network Agenda Setting Model and Issue Ownership. Mass Communication and Society, 18(5), 557-576. doi:10.1080/15205436.2015.1045300Harrington, S. (2008). Popular news in the 21st century Time for a new critical approach? Journalism: Theory, Practice & Criticism, 9(3), 266-284. doi:10.1177/1464884907089008López-Rico, C.-M., & Peris-Blanes, À. (2017). Agenda e imagen de los candidatos de las elecciones generales de 2015 en España en programas televisivos de infoentretenimiento. El Profesional de la Información, 26(4), 611. doi:10.3145/epi.2017.jul.05Maruyama, M., Robertson, S. P., Douglas, S., Raine, R., & Semaan, B. (2017). Social Watching a Civic Broadcast. Proceedings of the 2017 ACM Conference on Computer Supported Cooperative Work and Social Computing. doi:10.1145/2998181.2998340Medhat, W., Hassan, A., & Korashy, H. (2014). Sentiment analysis algorithms and applications: A survey. Ain Shams Engineering Journal, 5(4), 1093-1113. doi:10.1016/j.asej.2014.04.011Saif, H., He, Y., & Alani, H. (2012). Semantic Sentiment Analysis of Twitter. Lecture Notes in Computer Science, 508-524. doi:10.1007/978-3-642-35176-1_32Shah, D. V., Hanna, A., Bucy, E. P., Lassen, D. S., Van Thomme, J., Bialik, K., … Pevehouse, J. C. W. (2016). Dual Screening During Presidential Debates. American Behavioral Scientist, 60(14), 1816-1843. doi:10.1177/0002764216676245Sullivan, D. G., & Masters, R. D. (1988). «Happy Warriors»: Leaders’ Facial Displays, Viewers’ Emotions, and Political Support. American Journal of Political Science, 32(2), 345. doi:10.2307/2111127Thelwall, M., Buckley, K., Paltoglou, G., Cai, D., & Kappas, A. (2010). Sentiment strength detection in short informal text. Journal of the American Society for Information Science and Technology, 61(12), 2544-2558. doi:10.1002/asi.21416Vergeer, M., & Franses, P. H. (2015). Live audience responses to live televised election debates: time series analysis of issue salience and party salience on audience behavior. Information, Communication & Society, 19(10), 1390-1410. doi:10.1080/1369118x.2015.1093526Vilares, D., Thelwall, M., & Alonso, M. A. (2015). The megaphone of the people? Spanish SentiStrength for real-time analysis of political tweets. Journal of Information Science, 41(6), 799-813. doi:10.1177/0165551515598926Wohn, D. Y., & Na, E.-K. (2011). Tweeting about TV: Sharing television viewing experiences via social media message streams. First Monday. doi:10.5210/fm.v16i3.336

The professionals speak: Practitioners’ perspectives on professional election campaigning

Faced with some fundamental changes in the socio-cultural, political and media environment, political parties in post-industrialized democracies have started to initiate substantial transformations of both their organizational structures and communicative practices. Those innovations, described as professionalization, become most obvious during election campaigns. In recent times, the number of empirical studies measuring the degree of political parties’ campaign professionalism has grown. They have relied on a broad spectrum of indicators derived from theory which have not been tested for their validity. For the first time, we put these indicators to a ‘reality check’ by asking top-ranked party secretaries and campaign managers in 12 European countries to offer their perceptions of professional election campaigning. Furthermore, we investigate whether any differences in understanding professionalism among party campaign practitioners can be explained by macro (country) and meso (party) factors. By and large, our results confirm the validity of most indicators applied in empirical studies on campaign professionalism so far. There are some party- and country-related differences in assessing campaign professionalism too, but the influence of most factors on practitioners’ evaluations is weak. Therefore, we conclude that largely there is a far-reaching European Union-wide common understanding of professional election campaigning

Immer schlanker und kranker? Models in der Werbung

Es gibt eine mit Regelmäßigkeit und Vehemenz in den Massenmedien geführte Diskussion darüber, ob die mediale Darstellung so genannter „Magermodels" insbesondere für junge Frauen als eine Art Vorbild dienen könnte und spezifische Wirkungen nach sich zöge, etwa negative Körperbilder, Magersucht oder Anorexie. Die Debatte um diese oft als Tatsache gehandelte Frage und um mögliche (auch politische) Abhilfe fand erneut ihren Höhepunkt, als die Jury der Pro7-Sendung Germany’s Next Topmodel einer für den Normalbürger wohl als schlank geltenden Teilnehmerin erklärte, sie sei zu dick. Und so fragten ein weiteres Mal Öffentlichkeit und Politik den Models und ihren Auftraggebern Sommerlöcher in den Bauch, ob das denn so sein müsse und nicht auch anders gehe