PathOrganic – Risks and Recommendations Regarding Human Pathogens in Organic Vegetable Production Chains

PathOrganic assesses risks associated with the consumption of fresh and minimally processed vegetables due to the prevalence of bacterial human pathogens in plant produce. The project evaluates whether organic production poses a risk on food safety, taking into consideration sources of pathogen transmission (e.g. animal manure). The project also explores whether organic versus conventional production practices may reduce the risk of pathogen manifestation. In Europe, vegetable-linked outbreaks are not well investigated. A conceptual model together with novel sampling strategies and specifically adjusted methods provides the basis for large-scale surveys of organically grown plant produce in five European countries. Critical control points are determined and evaluated and factors contributing to a food safety problem are analyzed in greenhouse and field experiments. The project aims at developing a quantitative risk assessment model and at formulating recommendations for improving food safety in organic vegetable production

Diffusive Evolution of Stable and Metastable Phases II: Theory of Non-Equilibrium Behaviour in Colloid-Polymer Mixtures

By analytically solving some simple models of phase-ordering kinetics, we suggest a mechanism for the onset of non-equilibrium behaviour in colloid-polymer mixtures. These mixtures can function as models of atomic systems; their physics therefore impinges on many areas of thermodynamics and phase-ordering. An exact solution is found for the motion of a single, planar interface separating a growing phase of uniform high density from a supersaturated low density phase, whose diffusive depletion drives the interfacial motion. In addition, an approximate solution is found for the one-dimensional evolution of two interfaces, separated by a slab of a metastable phase at intermediate density. The theory predicts a critical supersaturation of the low-density phase, above which the two interfaces become unbound and the metastable phase grows ad infinitum. The growth of the stable phase is suppressed in this regime.Comment: 27 pages, Latex, eps

Educational paper: Defects in number and function of neutrophilic granulocytes causing primary immunodeficiency

The neutrophilic granulocyte (neutrophil) is the most important cellular component of the innate immune system. A total absence of neutrophils or a significant decrease in their number leads to severe immunodeficiency. A mature neutrophil, released from the bone marrow, should be able to migrate from the blood towards the tissues, following a chemotactic gradient to a pathogen. In order to be neutralized, this pathogen has to be recognized, phagocytosed, and destroyed by lytic enzymes contained in the neutrophil's granules and reactive oxygen species formed by the enzyme complex NADPH oxidase. Rare genetic defects leading to the loss of each one of these biological properties of the neutrophil have been described and are associated with immunodeficiency. This review provides a summary of the normal development and biological functions of neutrophils and describes the diseases caused by defects in neutrophil number and function

A radio ridge connecting two galaxy clusters in a filament of the cosmic web

Galaxy clusters are the most massive gravitationally bound structures in the Universe. They grow by accreting smaller structures in a merging process that produces shocks and turbulence in the intracluster gas. We observed a ridge of radio emission connecting the merging galaxy clusters Abell 0399 and Abell 0401 with the Low-Frequency Array (LOFAR) telescope network at 140 megahertz. This emission requires a population of relativistic electrons and a magnetic field located in a filament between the two galaxy clusters. We performed simulations to show that a volume-filling distribution of weak shocks may reaccelerate a preexisting population of relativistic particles, producing emission at radio wavelengths that illuminates the magnetic ridge

The coma cluster at low frequency array frequencies. I. Insights into particle acceleration mechanisms in the radio bridge

Radio synchrotron emission from the bridges of low-density gas connecting galaxy clusters and groups is a challenge for particle acceleration processes. In this work, we analyze the Coma radio bridge using new LOw Frequency ARray (LOFAR) observations at 144 MHz. LOFAR detects the bridge and its substructures with unprecedented sensitivity and resolution. We found that the radio emission peaks on the NGC 4839 group. Toward the halo, in front of the NGC 4839 group, the radio brightness decreases and streams of radio emission connect the NGC 4839 group to the radio relic. Using X-ray observations, we found that thermal and non-thermal plasma are moderately correlated with a sublinear scaling. We use archival radio data at 326 MHz to constrain the spectral index in the bridge, and quantify the distribution of particles and magnetic field at different frequencies. We found that the spectrum is steeper than −1.4 ± 0.2, and that the emission is clumpier at 326 MHz than at 144 MHz. Using cosmological simulations and a simplified approach to compute particle acceleration, we derive under which conditions turbulent acceleration of mildly relativistic electrons generate the radio emission in the bridge. Assuming that the initial energy ratio of the seed electrons is 3 · 10−4 with respect to the thermal gas, we are able to reproduce the observed luminosity. Our results suggest that the seed electrons released by radio galaxies in the bridge and the turbulence generated by the motion of gas and galaxies are essential to producing the radio emission

A radio ridge connecting two galaxy clusters in a filament of the cosmic web

Galaxy clusters are the most massive gravitationally bound structures in the Universe. They grow by accreting smaller structures in a merging process that produces shocks and turbulence in the intracluster gas. We observed a ridge of radio emission connecting the merging galaxy clusters Abell 0399 and Abell 0401 with the Low-Frequency Array (LOFAR) telescope network at 140 megahertz. This emission requires a population of relativistic electrons and a magnetic field located in a filament between the two galaxy clusters. We performed simulations to show that a volume-filling distribution of weak shocks may reaccelerate a preexisting population of relativistic particles, producing emission at radio wavelengths that illuminates the magnetic ridge

Understanding the radio relic emission in the galaxy cluster MACS J0717.5+3745: Spectral analysis

Radio relics are diffuse, extended synchrotron sources that originate from shock fronts generated during cluster mergers. The massive merging galaxy cluster MACS J0717.5+3745 hosts one of the more complex relics known to date. We present upgraded Giant Metrewave Radio Telescope band 3 (300-500 MHz) and band 4 (550-850 MHz) observations. These new observations, combined with published VLA and the new LOFAR HBA data, allow us to carry out a detailed, high spatial resolution spectral analysis of the relic over a broad range of frequencies. The integrated spectrum of the relic closely follows a power law between 144 MHz and 5.5 GHz with a mean spectral slope αâ€., =â€., -1.16†±â€ 0.03. Despite the complex morphology of this relic, its subregions and the other isolated filaments also follow power-law behaviors, and show similar spectral slopes. Assuming diffusive shock acceleration, we estimated a dominant Mach number of ∼3.7 for the shocks that make up the relic. A comparison with recent numerical simulations suggests that in the case of radio relics, the slopes of the integrated radio spectra are determined by the Mach number of the accelerating shock, with α nearly constant, namely between -1.13 and -1.17, for Mach numbers 3.5†-†4.0. The spectral shapes inferred from spatially resolved regions show curvature, we speculate that the relic is inclined along the line of sight. The locus of points in the simulated color-color plots changes significantly with the relic viewing angle. We conclude that projection effects and inhomogeneities in the shock Mach number dominate the observed spectral properties of the relic in this complex system. Based on the new observations we raise the possibility that the relic and a narrow-angle-tailed radio galaxy are two different structures projected along the same line of sight

An Efficient Strategy to Induce and Maintain In Vitro Human T Cells Specific for Autologous Non-Small Cell Lung Carcinoma

BACKGROUND: The efficient expansion in vitro of cytolytic CD8+ T cells (CTLs) specific for autologous tumors is crucial both for basic and translational aspects of tumor immunology. We investigated strategies to generate CTLs specific for autologous Non-Small Cell Lung Carcinoma (NSCLC), the most frequent tumor in mankind, using circulating lymphocytes. PRINCIPAL FINDINGS: Classic Mixed Lymphocyte Tumor Cultures with NSCLC cells consistently failed to induce tumor-specific CTLs. Cross-presentation in vitro of irradiated NSCLC cells by autologous dendritic cells, by contrast, induced specific CTL lines from which we obtained a high number of tumor-specific T cell clones (TCCs). The TCCs displayed a limited TCR diversity, suggesting an origin from few tumor-specific T cell precursors, while their TCR molecular fingerprints were detected in the patient's tumor infiltrating lymphocytes, implying a role in the spontaneous anti-tumor response. Grafting NSCLC-specific TCR into primary allogeneic T cells by lentiviral vectors expressing human V-mouse C chimeric TCRalpha/beta chains overcame the growth limits of these TCCs. The resulting, rapidly expanding CD4+ and CD8+ T cell lines stably expressed the grafted chimeric TCR and specifically recognized the original NSCLC. CONCLUSIONS: This study defines a strategy to efficiently induce and propagate in vitro T cells specific for NSCLC starting from autologous peripheral blood lymphocytes

Deep Low-frequency Radio Observations of A2256. I. the Filamentary Radio Relic

We present deep and high-fidelity images of the merging galaxy cluster A2256 at low frequencies using the upgraded Giant Metrewave Radio Telescope (uGMRT) and LOw-Frequency ARray (LOFAR). This cluster hosts one of the most prominent known relics with a remarkably spectacular network of filamentary substructures. The new uGMRT (300-850 MHz) and LOFAR (120-169 MHz) observations, combined with the archival Karl G. Jansky Very Large Array (VLA; 1-4 GHz) data, allowed us to carry out the first spatially resolved spectral analysis of the exceptional relic emission down to 6″ resolution over a broad range of frequencies. Our new sensitive radio images confirm the presence of complex filaments of magnetized relativistic plasma also at low frequencies. We find that the integrated spectrum of the relic is consistent with a single power law, without any sign of spectral steepening, at least below 3 GHz. Unlike previous claims, the relic shows an integrated spectral index of -1.07 ± 0.02 between 144 MHz and 3 GHz, which is consistent with the (quasi)stationary shock approximation. The spatially resolved spectral analysis suggests that the relic surface very likely traces the complex shock front, with a broad distribution of Mach numbers propagating through a turbulent and dynamically active intracluster medium. Our results show that the northern part of the relic is seen edge-on and the southern part close to face-on. We suggest that the complex filaments are regions where higher Mach numbers dominate the (re)acceleration of electrons that are responsible for the observed radio emission