Evaluation of Colloids and Activation Agents for Determination of Melamine Using UV-SERS

UV-SERS measurements offer a great potential for environmental or food (detection of food contaminats) analytics. Here, the UV-SERS enhancement potential of various kinds of metal colloids, such as Pd, Pt, Au, Ag, Au-Ag core-shell, and Ag-Au core-shell with different shapes and sizes, were studied using melamine as a test molecule. The influence of different activation (KF, KCl, KBr, K 2SO 4) agents onto the SERS activity of the nanomaterials was investigated, showing that the combination of a particular nanoparticle with a special activation agent is extremely crucial for the observed SERS enhancement. In particular, the size dependence of spherical nanoparticles of one particular metal on the activator has been exploited. By doing so, it could be shown that the SERS enhancement increases or decreases for increasing or decreasing size of a nanoparticle, respectively. Overall, the presented results demonstrate the necessity to adjust the nanoparticle size and the activation agent for different experiments in order to achieve the best possible UV-SERS results

Treatment of stage I-III periodontitis-The EFP S3 level clinical practice guideline

Background: The recently introduced 2017 World Workshop on the classification of periodontitis, incorporating stages and grades of disease, aims to link disease classification with approaches to prevention and treatment, as it describes not only disease severity and extent but also the degree of complexity and an individual's risk. There is, therefore, a need for evidence-based clinical guidelines providing recommendations to treat periodontitis. Aim: The objective of the current project was to develop a S3 Level Clinical Practice Guideline (CPG) for the treatment of Stage I–III periodontitis. Material and Methods: This S3 CPG was developed under the auspices of the European Federation of Periodontology (EFP), following the methodological guidance of the Association of Scientific Medical Societies in Germany and the Grading of Recommendations Assessment, Development and Evaluation (GRADE). The rigorous and transparent process included synthesis of relevant research in 15 specifically commissioned systematic reviews, evaluation of the quality and strength of evidence, the formulation of specific recommendations and consensus, on those recommendations, by leading experts and a broad base of stakeholders. Results: The S3 CPG approaches the treatment of periodontitis (stages I, II and III) using a pre-established stepwise approach to therapy that, depending on the disease stage, should be incremental, each including different interventions. Consensus was achieved on recommendations covering different interventions, aimed at (a) behavioural changes, supragingival biofilm, gingival inflammation and risk factor control; (b) supra- and sub-gingival instrumentation, with and without adjunctive therapies; (c) different types of periodontal surgical interventions; and (d) the necessary supportive periodontal care to extend benefits over time. Conclusion: This S3 guideline informs clinical practice, health systems, policymakers and, indirectly, the public on the available and most effective modalities to treat periodontitis and to maintain a healthy dentition for a lifetime, according to the available evidence at the time of publication

Evaluation of proximal contact tightness of Class II resin composite restorations.

Contains fulltext : 88644.pdf (publisher's version ) (Open Access)OBJECTIVE: The objective of the current study was to compare in-vitro the proximal contact tightness (PCT) of Class II resin composite restorations (RCR) placed with different established and new placement techniques. METHODS: 105 ivorine lower left first molars with standardized MO cavities were randomly divided into seven groups (n = 15) as follows: SRing: sectional matrix and separation ring (Garrison Dental); CRing: circumferential matrix (1101-c, KerrHawe SA) with separation ring; CWedge: circumferential matrix with a wedge only; COptra: circumferential matrix and OptraContact (Vivadent); CCerana: circumferential matrix and a Cerana insert (Nordiska Dental); CElliot: circumferential matrix and Elliot separator (PFINGST & Co) and Walser: Walser matrix O-type (Dr Walser Dental GmbH). In all the groups, the matrix band was secured using a wooden wedge except for the Walser group, following manufacturer's recommendations. A Tofflemire retainer (Kerr Corporation) was used to apply the circumferential matrix band whenever it was used. All the prepared teeth were restored with resin composite (Premise, Kerr) mounted in a manikin head to simulate the clinical environment. PCT was measured using the Tooth Pressure Meter (University of Technology, Delft). The data were analyzed using one-way ANOVA and Tukey post-hoc tests (p < 0.05). RESULTS: Compared to the control group (SRing) (6.64 +/- 1.06N), all other systems resulted in significantly lower PCT values (p < 0.001). Within the circumferential matrix groups, CRing (4.01 +/- 0.53N) and CElliot (4.29 +/- 1.08N) showed significantly tighter contacts compared to the CWedge (0.37 +/- 0.22N), COptra (0.91 +/- 0.49N), CCerana (2.99 +/- 1.98N) and Walser (1.34 +/- 0.55N) (p < 0.05) group. Between CWedge and COptra, no significant difference was found (p = 0.57). CONCLUSION: The use of separation rings with sectional matrices provides superior contacts when placing Class II RCRs

Comparison of proximal contacts of class II resin composite restorations in vitro.

Item does not contain fulltextThis study investigated the tightness of the proximal contact when placing posterior resin composite restorations with circumferential and sectional matrix systems in an in vitro model using a special measuring device (Tooth Pressure Meter). A manikin model was used with an artificial first molar in which an MO-preparation was ground, simulating the clinical situation of an amalgam replacement. This preparation was duplicated, resulting in 160 identically prepared teeth. These teeth were divided into 8 groups (n=20). In 2 groups, circumferential matrix bands (flat or contoured) in a Tofflemire retainer were applied. In the remaining 6 groups, 3 different separation rings were combined with 2 types of sectional matrix bands. All the cavities were restored using Clearfil Photo Bond and Clearfil AP-X. The tightness of the proximal contact was measured using the Tooth Pressure Meter. Data were statistically analyzed using SPSS 12. ANOVA was used to find differences in proximal contact tightness between the groups. Tukey tests were used to find differences between the homogeneous subgroups. The use of sectional matrices combined with separation rings resulted in tighter proximal contacts compared to when circumferential systems were used (p<0.001). The use of these devices is therefore recommended when posterior resin composite restorations are placed

A clinical study on interdental separation techniques.

Contains fulltext : 52502.pdf (publisher's version ) (Closed access)The effect of interdental separation of a special separation ring and wooden wedge was investigated. In a split-mouth design, 27 patients were randomly assigned to one of two groups (W or S). In 11 patients, an interdental wooden wedge (Hawe-Neos) was placed (group W), and in 16 patients, a separation ring (Composi-Tight Gold) was placed at the contact between teeth 4/5 and 5/6. Simultaneously, in both groups, a wooden wedge, combined with a separation ring (Composi-Tight Gold), was placed on the contact between teeth 4/5 and 5/6 (reference group W+S). To measure proximal contact tightness, frictional forces were recorded at the removal of a 0.05 mm thick metal matrix band inserted between adjacent teeth. Contact tightness was measured at contacts 4 and 5 and at 5 and 6 in the third and fourth quadrant using the Tooth Pressure Meter prior to applying separation devices (TO) five minutes after application (T1) and five minutes after removal of the devices (T2). The effect of separation was determined by calculating the differences between contact tightness before application and contact tightness with the devices in situ (T1-T0). Interdental recovery was calculated by the difference in contact tightness before application and after removal of the devices (T2-T0). To assess the presence of statistically significant differences between these measurement times, paired t-tests were applied. With each patient, either a comparison between W and W+S or S and W+S was made. For both W versus W+S and S versus W+S, paired t-tests were applied to compare differences (T1-T0 and T2-T0) between the separation devices. Within a patient, groups W and S could not be compared, therefore, to compare separation achieved between these two devices, unpaired t-tests were used. The increase in contact tightness measured at contact 4 and 5 for group W (0.98 +/- 0.26 N) was statistically significantly less compared to the increase in group S (5.48 +/- 0.88 N) (p < 0.001) or group W+S (4.62 +/- 0.68 N) (p = 0.02). No significant differences were found between groups S and W+S (p = 0.77). For all groups, five minutes after removal of the devices, the contact tightness at contact 4 and 5 and at contact 5 and 6 were still significantly weaker compared to the tightness at baseline (p < 0.02). When separation is required for restorative procedures, such as at placement of a Class II resin composite restoration, special separation rings may be more useful than wooden wedges

The impact of intrinsic and extrinsic factors on the job satisfaction of dentists

The Two-Factor Theory of job satisfaction distinguishes between intrinsic-motivation (i.e. recognition, responsibility) and extrinsic-hygiene (i.e. job security, salary, working conditions) factors. The presence of intrinsic-motivation facilitates higher satisfaction and performance, whereas the absences of extrinsic factors help mitigate against dissatisfaction. The consideration of these factors and their impact on dentists’ job satisfaction is essential for the recruitment and retention of dentists.Objectives: The objective of the study is to assess the level of job satisfaction of German dentists and the factors that are associated with it.Methods: This cross-sectional study was based on a job satisfaction survey. Data were collected from 147 dentists working in 106 dental practices. Job satisfaction was measured with the 10-item Warr-Cook-Wall job satisfaction scale. Organizational characteristics were measured with two items. Linear regression analyses were performed in which each of the nine items of the job satisfaction scale (excluding overall satisfaction) were handled as dependent variables. A stepwise linear regression analysis was performed with overall job satisfaction as the dependent outcome variable, the nine items of job satisfaction and the two items of organizational characteristics controlled for age and gender as predictors.Results: The response rate was 95.0%. Dentists were satisfied with ‘freedom of working method’ and mostly dissatisfied with their ‘income’. Both variables are extrinsic factors. The regression analyses identified five items that were significantly associated with each item of the job satisfaction scale: ‘age’, ‘mean weekly working time’, ‘period in the practice’, ‘number of dentist's assistant’ and ‘working atmosphere’. Within the stepwise linear regression analysis the intrinsic factor ‘opportunity to use abilities’ (β = 0.687) showed the highest score of explained variance (R2 = 0.468) regarding overall job satisfaction.Conclusions: With respect to the Two-Factor Theory of job satisfaction both components, intrinsic and extrinsic, are essential for dentists but the presence of intrinsic motivating factors like the opportunity to use abilities has most positive impact on job satisfaction. The findings of this study will be helpful for further activities to improve the working conditions of dentists and to ensure quality of car

Oral Mesenchymal Stem/Progenitor Cells: The Immunomodulatory Masters

Oral mesenchymal stem/progenitor cells (MSCs) are renowned in the field of tissue engineering/regeneration for their multilineage differentiation potential and easy acquisition. These cells encompass the periodontal ligament stem/progenitor cells (PDLSCs), the dental pulp stem/progenitor cells (DPSCs), the stem/progenitor cells from human exfoliated deciduous teeth (SHED), the gingival mesenchymal stem/progenitor cells (GMSCs), the stem/progenitor cells from the apical papilla (SCAP), the dental follicle stem/progenitor cells (DFSCs), the bone marrow mesenchymal stem/progenitor cells (BM-MSCs) from the alveolar bone proper, and the human periapical cyst-mesenchymal stem cells (hPCy-MSCs). Apart from their remarkable regenerative potential, oral MSCs possess the capacity to interact with an inflammatory microenvironment. Although inflammation might affect the properties of oral MSCs, they could inversely exert a multitude of immunological actions to the local inflammatory microenvironment. The present review discusses the current understanding about the immunomodulatory role of oral MSCs both in periodontitis and systemic diseases, their “double-edged sword” uniqueness in inflammatory regulation, their affection of the immune system, and the underlying mechanisms, involving oral MSC-derived extracellular vesicles