Deletion mutants in COP9/Signalosome subunits in fission yeast Schizosaccharomyces pombe display distinct phenotypes

The COP9/signalosome complex is highly conserved in evolution and possesses significant structural similarity to the 19S regulatory lid complex of the proteasome. It also shares limited similarity to the translation initiation factor eIF3. The signalosome interacts with multiple cullins in mammalian cells. In the fission yeast Schizosaccharomyces pombe, the Csn1 subunit is required for the removal of covalently attached Nedd8 from Pcu1, one of three S. pombe cullins. It remains unclear whether this activity is required for all the functions ascribed to the signalosome. We previously identified Csn1 and Csn2 as signalosome subunits in S. pombe. csn1 and csn2 null mutants are DNA damage sensitive and exhibit slow DNA replication. Two further putative subunits, Csn4 and Csn5, were identified from the S. pombe genome database. Herein, we characterize null mutations of csn4 and csn5 and demonstrate that both genes are required for removal of Nedd8 from the S. pombe cullin Pcu1 and that their protein products associate with Csn1 and Csn2. However, neither csn4 nor csn5 null mutants share the csn1 and csn2 mutant phenotypes. Our data suggest that the subunits of the signalosome cannot be considered as a distinct functional unit and imply that different subunits of the signalosome mediate distinct functions

Facilities for meteorological research at NASA Goddard/Wallops Flight Facility

The technical characteristics of the Atmospheric Sciences Research Facility, the improvements being made to the instrumentation there which will enhance its usefulness in atmospheric research, and several of the on-going research programs are described. Among the area of atmospheric research discussed are clouds and precipitation, lightning, ozone, wind, and storms. Meteorological instruments including Doppler radar, spectrophotometers, and ozone sensors are mentioned. Atmospheric research relevant to aircraft design and COMSTAR communication satellites is briefly discussed

Space station architectural elements model study

The worksphere, a user controlled computer workstation enclosure, was expanded in scope to an engineering workstation suitable for use on the Space Station as a crewmember desk in orbit. The concept was also explored as a module control station capable of enclosing enough equipment to control the station from each module. The concept has commercial potential for the Space Station and surface workstation applications. The central triangular beam interior configuration was expanded and refined to seven different beam configurations. These included triangular on center, triangular off center, square, hexagonal small, hexagonal medium, hexagonal large and the H beam. Each was explored with some considerations as to the utilities and a suggested evaluation factor methodology was presented. Scale models of each concept were made. The models were helpful in researching the seven beam configurations and determining the negative residual (unused) volume of each configuration. A flexible hardware evaluation factor concept is proposed which could be helpful in evaluating interior space volumes from a human factors point of view. A magnetic version with all the graphics is available from the author or the technical monitor

Study of process variables associated with manufacturing hermetically sealed nickel-cadium cells Quarterly report, 23 May - 23 Aug. 1970

Separator materials, ceramic to metal seals, cell plate polarization and impregnation processes, and plaque sintering data for study of variables in manufacture of nickel cadmium cell

Continued evaluation of potential for geologic storage of carbon dioxide in the southeastern United States

Southern States Energy Board Duke Energy Santee Cooper Power Southern CompanyBureau of Economic Geolog

Macroscopic quantum tunnelling of Bose-Einstein condensates in a finite potential well

Bose-Einstein condensates are studied in a potential of finite depth which supports both bound and quasi-bound states. This potential, which is harmonic for small radii and decays as a Gaussian for large radii, models experimentally relevant optical traps. The nonlinearity, which is proportional to both the number of atoms and the interaction strength, can transform bound states into quasi-bound ones. The latter have a finite lifetime due to tunnelling through the barriers at the borders of the well. We predict the lifetime and stability properties for repulsive and attractive condensates in one, two, and three dimensions, for both the ground state and excited soliton and vortex states. We show, via a combination of the variational and WKB approximations, that macroscopic quantum tunnelling in such systems can be observed on time scales of 10 milliseconds to 10 seconds.Comment: J. Phys. B: At. Mol. Opt. Phys. in pres

Stringy Effects During Inflation and Reheating

We consider inflationary cosmology in the context of string compactifications with multiple throats. In scenarios where the warping differs significantly between throats, string and Kaluza-Klein physics can generate potentially observable corrections to the cosmology of inflation and reheating. First we demonstrate that a very low string scale in the ground state compactification is incompatible with a high Hubble scale during inflation, and we propose that the compactification geometry is altered during inflation. In this configuration, the lowest scale is just above the Hubble scale, which is compatible with effective field theory but still leads to potentially observable CMB corrections. Also in the appropriate region of parameter space, we find that reheating leads to a phase of long open strings in the Standard Model sector (before the usual radiation-dominated phase). We sketch the cosmology of the long string phase and we discuss possible observational consequences.Comment: 33pp, RevTeX4, v2. minor changes, added ref

Semi-Static Hedging Based on a Generalized Reflection Principle on a Multi Dimensional Brownian Motion

On a multi-assets Black-Scholes economy, we introduce a class of barrier options. In this model we apply a generalized reflection principle in a context of the finite reflection group acting on a Euclidean space to give a valuation formula and the semi-static hedge.Comment: Asia-Pacific Financial Markets, online firs

Conceptual design of an orbital propellant transfer experiment. Volume 2: Study results

The OTV configurations, operations and requirements planned for the period from the 1980's to the 1990's were reviewed and a propellant transfer experiment was designed that would support the needs of these advanced OTV operational concepts. An overall integrated propellant management technology plan for all NASA centers was developed. The preliminary cost estimate (for planning purposes only) is $56.7 M, of which approximately$31.8 M is for shuttle user costs