Ensaios de integridade estrutural de placas angulares de fixação de fracturas ósseas do fémur

Neste trabalho descreve-se o projecto de um dispositivo mecânico desenvolvido para a realização de ensaios segundo a norma ASTM F384-00 (2000) de placas de osteossíntese anguladas. O dispositivo foi objecto de uma análise estrutural usando o método dos elementos finitos. Algumas placas foram ensaiadas estaticamente e suas características mecânicas, de acordo com a norma, determinadas

A meteorologia na interpretaçao física da agudizaçao da asma: uma amostra termal

Ponencia presentada en: XXIX Jornadas Científicas de la AME y el VII Encuentro Hispano Luso de Meteorología celebrado en Pamplona, del 24 al 26 de abril de 2006.O efeito de águas termais na saúde tem sido aceite pela maioria dos cientistas que investigam nesta temática, como tem sido demonstrado pela International Society of Medical Hydrology and Climatology. A água mineral das Termas das Caldas da Felgueira tem apresentado, ao longo dos anos, indicações terapêuticas no que respeita a diversas doenças do foro respiratório, nomeadamente a asma brônquica e a asma crónica. Este trabalho apresenta os primeiros resultados derivados da análise de uma amostra, que procura nas termas o alívio para as inesperadas agudizações da asma ao longo do ano. Neste estudo estamos particularmente interessados em conhecer o padrão elegível da amostra, que recorre a tratamentos realizados nas Termas das Caldas-da-Felgueira

CO2 dissolution and design aspects of a multiorifice oscillatory baffled column

Dissolution of CO2 in water was studied for a batch vertical multiorifice baffled column (MOBC) with varying orifice diameters (d0) of 6.4-30 mm and baffle open area (α) of 15-42%. Bubble size distributions (BSDs) and the overall volumetric CO2 mass transfer coefficient (KLa) were experimentally evaluated for very low superficial gas velocities, UG of 0.12-0.81 mm s-1, using 5% v/v CO2 in the inlet gas stream at a range of fluid oscillations (f = 0-10 Hz and x0 = 0-10 mm). Remarkably, baffles presenting large do = 30 mm and α = 36%, therefore in the range typically found for single-orifice oscillatory baffled columns, were outperformed with respect to BSD control and CO2 dissolution by the other baffle designs or the same aerated column operating without baffles or fluid oscillations. Flow visualization and bubble tracking experiments also presented in this study established that a small do of 10.5 mm combined with a small value of α = 15% generates sufficient, strong eddy mixing capable of generating and trapping an extremely large fraction of microbubbles in the MOBC. This resulted in increased interfacial area yielding KLa values up to 65 ± 12 h-1 in the range of the UG tested, representing up to 3-fold increase in the rate of CO2 dissolution when compared to the unbaffled, steady column. In addition, a modi fied oscillatory Reynolds number, Re′o and Strouhal number, St' were presented to assist on the design and scale-up of gas-liquid systems based on multiorifice oscillatory ba ffled columns. This work is relevant to gas-liquid or multiphase chemical and biological systems relying on efficient dissolution of gaseous compounds into a liquid medium.BBSRC -European Commissio

The α-Arrestin Bul1p Mediates Lactate Transporter Endocytosis in Response to Alkalinization and Distinct Physiological Signals

Eukaryotic α-arrestins connect environmental or stress signaling pathways to the endocytosis of plasma membrane transporters or receptors. The Saccharomyces cerevisiae lactate transporter Jen1p has been used as a model cargo for elucidating the mechanisms underlying endocytic turnover in response to carbon sources. Here, we discover a novel pathway of Jen1p endocytosis mediated by the α-arrestin Bul1p in response to the presence of cycloheximide or rapamycin, or prolonged growth in lactate. While cycloheximide or rapamycin modify cells pleiotropically, the major effect of prolonged growth in lactate was shown to be external pH alkalinization. Importantly, employment of specific inactive Jen1p versions showed that Bul1p-dependent endocytosis requires lactate transport, according to the signal imposed. Our results support a model where conformational changes of Jen1p, associated with substrate/H+ symport, are critical for the efficiency of Bul1p-dependent Jen1p turnover. © 2017 Elsevier Lt

GPCRs: Heterodimer-specific signaling

SCOPUS: no.jinfo:eu-repo/semantics/publishe

Learning from critical internationalisation events. Insights from two fast growing Italian SMEs

At present, Europe lacks innovative companies, which grow quickly and end up big. This book examines SME growth, innovation and success, to suggest that fast-growing firms could offer a major contribution to the recovery of a European economy. The contributors examine eleven case studies from Italian firms, breaking the book up into three parts: context, actors and strategy. The topics discussed include entrepreneurship and technological clusters, innovative start-ups and growth factors, and family firms as the incubators of new ventures. Students and scholars of entrepreneurship and other related disciplines will find this book to be of interest. It will also be of use to practitioners in the field, working with and alongside SMEs

Interactions of cytosolic tails in the Jen1 carboxylate transporter are critical for trafficking and transport activity

Plasma membrane (PM) transporters of the major facilitator superfamily (MFS) are essential for cell metabolism, growth and response to stress or drugs. In Saccharomyces cerevisiae, Jen1 is a monocarboxylate/H+ symporter that provides a model to dissect the molecular details underlying cellular expression, transport mechanism and turnover of MFS transporters. Here, we present evidence revealing novel roles of the cytosolic N- and C-termini of Jen1 in its biogenesis, PM stability and transport activity, using functional analyses of Jen1 truncations and chimeric constructs with UapA, an endocytosis-insensitive transporter of Aspergillus nidulans. Our results show that both N- and C-termini are critical for Jen1 trafficking to the PM, transport activity and endocytosis. Importantly, we provide evidence that Jen1 N- and C-termini undergo transport-dependent dynamic intramolecular interactions, which affect the transport activity and turnover of Jen1. Our results support an emerging concept where the cytoplasmic termini of PM transporters control transporter cell surface stability and function through flexible intramolecular interactions with each other. These findings might be extended to other MFS members to understand conserved and evolving mechanisms underlying transporter structure-function relationships. This article has an associated First Person interview with the first authors of the paper. © 2022. Published by The Company of Biologists Ltd