Growth performance, morphometric analysis of the intestinal mucosa and thyroid of broiler fed canola meal.

Abstract: The aim of this study was to evaluate the effect of replacing soybean meal with canola meal in broiler diets on performance, liver histopathology, morphometry of the intestinal mucosa and thyroid. One-day-old Cobb chicks (n=300) were distributed in a completely randomized design, with increasing levels of replacement of soybean meal with canola meal (0, 25, 50, 75 and 100%) and 6 repetitions of 10 birds each. Weight gain decreased linearly (P< 0.05) with increasing levels of inclusion of canola meal, in all stages. Villus height in the duodenum and jejunum linearly decreased (P< 0.05). Follicle diameter and thyroid follicular epithelium height increased linearly with increasing levels of canola meal (P< 0.05). Similarly, there was an increase in relative weight of liver and heart, and liver steatosis in the highest levels of replacement. In conclusion, the replacement of soybean meal with canola meal can reduce performance, adversely affecting the thyroid, liver and the morphometric characteristics in the duodenum and jejunum. Resumo: O objetivo deste estudo foi avaliar o efeito da substituição do farelo de soja pelo farelo de canola na dieta de frangos de corte sobre o desempenho, a histopatologia hepática, a morfometria da mucosa intestinal e da tiroide. Foram distribuídos 300 pintos com um dia de idade em um delineamento inteiramente causualizado, com níveis crescentes de substituição do farelo de soja pelo farelo de canola (0, 25, 50, 75 e 100%), 6 repetições com 10 aves cada. O ganho de peso diminuiu linearmente (P< 0,05) com níveis crescentes da substituição, em todas as fases. Assim como, a altura das vilosidades no duodeno e jejuno diminuiu linearmente (P< 0,05) em todas as idades avaliadas. O diâmetro do folículo e a altura do epitélio da tireoide aumentaram linearmente conforme aumentou os níveis de farelo de canola (P< 0,05). Da mesma forma, houve um aumento do peso relativo do fígado e do coração, e aparecimento de esteatose hepática nos níveis mais altos de substituição. Em conclusão, a substituição do farelo de soja pelo farelo de canola pode reduzir o desempenho, afetando negativamente a tireoide, o fígado e as características morfométricas no duodeno e jejuno

Economic Fluctuations and Diffusion

Stock price changes occur through transactions, just as diffusion in physical systems occurs through molecular collisions. We systematically explore this analogy and quantify the relation between trading activity - measured by the number of transactions $N_{\Delta t}$ - and the price change $G_{\Delta t}$, for a given stock, over a time interval $[t, t+\Delta t]$. To this end, we analyze a database documenting every transaction for 1000 US stocks over the two-year period 1994-1995. We find that price movements are equivalent to a complex variant of diffusion, where the diffusion coefficient fluctuates drastically in time. We relate the analog of the diffusion coefficient to two microscopic quantities: (i) the number of transactions $N_{\Delta t}$ in $\Delta t$, which is the analog of the number of collisions and (ii) the local variance $w^2_{\Delta t}$ of the price changes for all transactions in $\Delta t$, which is the analog of the local mean square displacement between collisions. We study the distributions of both $N_{\Delta t}$ and $w_{\Delta t}$, and find that they display power-law tails. Further, we find that $N_{\Delta t}$ displays long-range power-law correlations in time, whereas $w_{\Delta t}$ does not. Our results are consistent with the interpretation that the pronounced tails of the distribution of $G_{\Delta t} are due to$w_{\Delta t}$, and that the long-range correlations previously found for$| G_{\Delta t} |$are due to$N_{\Delta t}$.Comment: RevTex 2 column format. 6 pages, 36 references, 15 eps figure

Dynamic functional connectivity in migraine during the interictal phase: a resting-state fMRI study

Question: Migraine is a cyclic and complex disorder, characterized by attacks of headache, sensory and cognitive disturbances1. Thalamocortical connectivity in migraine has been found to be transiently abnormal2. Our aim was to assess if the dynamical properties of the migraine brain are affected during the interictal phase. Methods: Resting-state functional MRI data was collected from 14 menstrual migraine patients without aura (interictal phase) and 12 healthy controls (menstrual post-ovulation phase). fMRI data processing included3: motion and distortion correction, temporal highpass filter, regression of motion and physiological confounds, spatial smoothing, and parcellation with the Desikan atlas. Dynamic functional connectivity (dFC) between regions was computed using phase coherence, and recurrent dFC states were identified by kmeans clustering (k ranging between 3 and 15) of the leading eigenvectors of dFC in each time point4. Permutation tests were performed to evaluate statistically significant differences between patients and controls in the probability of occurrence and the mean lifetime of the dFC states. Results: Similar dFC states were found consistently across different numbers of clusters, k, which resembled the canonical resting-state networks as expected. Compared to healthy controls, migraine patients show a significantly lower mean lifetime in one dFC state, when grouping in 4, 5 and 6 clusters. No differences were found for the probability of occurrence. Conclusions: Migraine may be linked to a disruption of brain networks dynamics. This emphasizes the need to adopt time-resolved methods, in addition to static, to study functional connectivity, to better understand the mechanisms of migraine. Our next step will be to assess the dynamics of the migraine brain throughout the migraine cycle.info:eu-repo/semantics/publishedVersio

Effect of postemergence herbicides on agronomics characteristics of soybean cv. Emgopa 316

Conduziu-se um ensaio na fazenda Bela Vista, município de Serranópolis-GO, objetivando avaliar os efeitos de latifolicidas aplicados em pós-emergência, sobre algumas características agronômicas da soja cv. Emgopa 316, com ciclo de maturação precoce. Os tratamentos testados foram: lactofen (168 g/ha), chlorimuron-ethyl (15 g/ha) + óleo mineral (0,2 v/v), fomesafen (250 g/ha) + óleo mineral (0,2 v/v), imazethapyr (100 g/ha) + óleo mineral (0,2% v/v), chlorimuronethyl+ lactofen (12,5+96 g/ha), chlorimuron-ethyl+fomesafen (12,5+ 150 g/ha), chlorirnuron-ethyl+imazethapyr (12,5+50 g/ha), imazethapyr+lactofen (50+96 g/ha), imazethapyr+fomesafen (50+ 150 g/ha), além da testemunha. Os herbicidas, nas doses estudadas, provocaram injúrias às plantas de soja cv. Emgopa 316, com maior intensidade nos tratamentos contendo lactofen, porém não intluenciaram na altura das plantas aos 15,30 e 45 DAT e na colheita da soja. Também não afetaram a altura de inserção da primeira vagem, densidade de plantas, número de vagens/planta, peso de 1000 sementes e nem o rendimento de grãos. A mistura imazethapyr+fomesafen (50+ 150 g/ha) proporcionou o maior número de entrenós, porém, não diferiu estatisticamente dos demais tratamentos, exceto para lactofen (168 g/ha), que proporcionou o menor número de entrenós. O herbicida lactofen isolado ou em combinação com chlorimuron-ethyl ou com imazethapyr, pode se utilizado em soja cv. Emgopa 316, com ciclo de maturação precoce, apresentando resultados semelhantes ao fomesafen isolado ou em mistura com chlorimuron-ethyl ou imazethapyr.  A experiment was carried out at the Bela Vista Farm, in Serranópolis, State ofGoiás, with the objective to evaluate the effects of postemergence herbicides on agronomic characteristcs of soybean cv. Emgopa 316, with premature maturation cycle. Treatments consisted oflactofen (168 glha), chlorimuron-ethyl (15 glha) + mineral oil (0.2 v/v), fomesafen (250 g/ha) + mineral oil (0.2 v/v), imazethapyr (100 g/ha), chlorimuron-ethyl+lactofen (12.5+96 g/ha), chlorimuronethyl+ fomesafen (12.5+ 150 g/ha), chlorirnuron-ethyl+imazethapyr (12.5+50 g/ha), imazethapyr+lactofen (50+96 g/ha), imazethapyr+fomesafen (50+ 150 g/ha), and a handweeded control. The studied herbicide doses caused some injury to soybean, with more intensity in the treatments with lactofen. However, they did not significantly intluence the height of plants at 15, 30 and 45 days after treatments and at soybean harvest. The herbicides also did not significantly affect insertion height of the first pod, final stand, number of podslplant, weight of 1000 seeds and grain production. Imazethapyr+fomesafen (50+ 150 g/ha) gave the best result in number of internodes, but it did not differ statistically from the other treatments, except for lactofen (168 g/ha), that proportioned the lowest number of internodes. Lactofen isolated or combined with chlorimuron-ethyl or with imazethapyr can be used in soybean cv. Emgopa 316, showing results similar to fomesafen isolated or in mixture with chlorimuron-ethyl or imazethapyr

Blind Testing: DNA Barcoding Sheds Light Upon the Identity of Plant Fragments as a Subsidy for Cave Conservation

Plants living above and around caves represent an important, albeit poorly studied, resource within cave ecosystems. The presence of plant material (root-like structures or rhizothemes, saplings, seeds, and seedlings) correlates positively with the biodiversity of the cave dwelling animals as shown for iron-ore caves in Carajás, Pará, Brazil. Plant material collected in caves has proven to be difficult to identify by traditional botanical methods, thus this research aims to provide a qualitative insight into the taxonomy and morphology of rhizothemes and other plant fragments found in the caves. The identification process used a combination of different molecular markers (ITS2, rbcL, and trnH-psbA) followed by a comparison of the sequences obtained against publicly available databases. The rhizothemes were submitted to micromorphological analysis to ascertain their putative root or stem origin and to compare their anatomy with known patterns found in the plant families or genera recovered through molecular matches. All studied samples were Angiosperms, mostly belonging to subclass Rosideae, within four orders: Malpighiales (Euphorbiaceae, Hypericaceae), Sapindales (Anacardiaceae and Sapindaceae), Myrtales (Myrtaceae), Fabales (Fabaceae), and only two belonging to subclass Asteridae, order Gentianales (Apocynaceae). Some of the samples were matched to generic level, with ITS2 being the best marker to identify the fragments because it shows high degree of sequence variation even at specific level and result reliability. All rhizothemes turned out to be roots, and correspondence was found between the existing literature and the individual anatomical patterns for the families and genera retrieved. DNA barcode has proved to be a useful tool to identify plant fragments found in this challenging environment. However, the existence of well curated, authoritatively named collections with ample biological information has proven to be essential to achieve a reliable identification

Sugar-based bactericides targeting phosphatidylethanolamine-enriched membranes

Free PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6242839/Anthrax is an infectious disease caused by Bacillus anthracis, a bioterrorism agent that develops resistance to clinically used antibiotics. Therefore, alternative mechanisms of action remain a challenge. Herein, we disclose deoxy glycosides responsible for specific carbohydrate-phospholipid interactions, causing phosphatidylethanolamine lamellar-to-inverted hexagonal phase transition and acting over B. anthracis and Bacillus cereus as potent and selective bactericides. Biological studies of the synthesized compound series differing in the anomeric atom, glycone configuration and deoxygenation pattern show that the latter is indeed a key modulator of efficacy and selectivity. Biomolecular simulations show no tendency to pore formation, whereas differential metabolomics and genomics rule out proteins as targets. Complete bacteria cell death in 10 min and cellular envelope disruption corroborate an effect over lipid polymorphism. Biophysical approaches show monolayer and bilayer reorganization with fast and high permeabilizing activity toward phosphatidylethanolamine membranes. Absence of bacterial resistance further supports this mechanism, triggering innovation on membrane-targeting antimicrobials.The European Union is gratefully acknowledged for the support of the project “Diagnostic and Drug Discovery Initiative for Alzheimer’s Disease” (D3i4AD), FP7-PEOPLE-2013-IAPP, GA 612347. We thank the Management Authorities of the European Regional Development Fund and the National Strategic Reference Framework for the support of the Incentive System - Research and Technological Development Co-Promotion FACIB Project number 21457. Fundação para a Ciência e a Tecnologia is also acknowledged for the support of projects UID/Multi/00612/2013, FCT/UID/ Multi/04046/2013, IF/00808/2013/CP1159/CT0003, PTDC/BBBBQB/6071/2014, as well as for the post-doc grant SFRH/BPD/42567/2007 (A.M.), the Ph.D. grants SFRH/BDE/51998/2012 (C.D.), and SFRH/BDE/51957/2012 (J.P.P.), both co-sponsored by CIPAN, and also for the Ph.D. grant SFRH/BD/116614/2016 (R.N.).info:eu-repo/semantics/publishedVersio