The occurrence of unpigmented mature oocytes in Hypsiboas (Anura: Hylidae)

The genus Hypsiboas is composed of 90 species distributed in Central and South America from Nicaragua to Argentina, as well as on Trinidad and Tobago (Frost 2014). Previous studies on the reproductive biology of species in this genus have reported that female mature oocytes or eggs generally have pigmented animal poles (Tab. 1), which has been generally suggested as a protection of the exposed embryos from solar radiation (Wells 2007).Fil: Nali, Renato C.. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Faivovich, Julián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Prado, Cynthia P. A.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasi

Efeito de substratos e recipientes na aclimatização de goiabeiras Paluma multiplicadas in vitro.

O objetivo desse trabalho foi determinar oefeito de diferentes tipos de substratos e recipientes na aclimatização de goiabeira Paluma multiplicadas in vitro

Efficiency of natural insecticides and thiamethoxam on the control of thrips in grapes and selectivity to natural enemies.

The objective of this work was to verify the efficiency of natural insecticides and thiamethoxan on the control of Selenothrips rubrocinctus and Frankliniella sp. and their selectivity to natural enemies

Micropropagação e aclimatização de goiabeira Paluma.

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Campus smoking policies and smoking-related Twitter posts originating from California public universities: Retrospective study

BACKGROUND: The number of colleges and universities with smoke- or tobacco-free campus policies has been increasing. The effects of campus smoking policies on overall sentiment, particularly among young adult populations, are more difficult to assess owing to the changing tobacco and e-cigarette product landscape and differential attitudes toward policy implementation and enforcement. OBJECTIVE: The goal of the study was to retrospectively assess the campus climate toward tobacco use by comparing tweets from California universities with and those without smoke- or tobacco-free campus policies. METHODS: Geolocated Twitter posts from 2015 were collected using the Twitter public application programming interface in combination with cloud computing services on Amazon Web Services. Posts were filtered for tobacco products and behavior-related keywords. A total of 42,877,339 posts were collected from 2015, with 2837 originating from a University of California or California State University system campus, and 758 of these manually verified as being about smoking. Chi-square tests were conducted to determine if there were significant differences in tweet user sentiments between campuses that were smoke- or tobacco-free (all University of California campuses and California State University, Fullerton) compared to those that were not. A separate content analysis of tweets included in chi-square tests was conducted to identify major themes by campus smoking policy status. RESULTS: The percentage of positive sentiment tweets toward tobacco use was higher on campuses without a smoke- or tobacco-free campus policy than on campuses with a smoke- or tobacco-free campus policy (76.7% vs 66.4%, P=.03). Higher positive sentiment on campuses without a smoke- or tobacco-free campus policy may have been driven by general comments about one’s own smoking behavior and comments about smoking as a general behavior. Positive sentiment tweets originating from campuses without a smoke- or tobacco-free policy had greater variation in tweet type, which may have also contributed to differences in sentiment among universities. CONCLUSIONS: Our study introduces preliminary data suggesting that campus smoke- and tobacco-free policies are associated with a reduction in positive sentiment toward smoking. However, continued expressions and intentions to smoke and reports of one’s own smoking among Twitter users suggest a need for more research to better understand the dynamics between implementation of smoke- and tobacco-free policies and resulting tobacco behavioral sentiment

Mushroom-Based Supplements in Italy: Let’s Open Pandora’s Box

Mushrooms and derivates are well known to the scientific community for having different health benefits and exhibit a wide range of pharmacological activities, including lipid-lowering, antihypertensive, antidiabetic, antimicrobic, antiallergic, anti-inflammatory, anticancer, immunomodulating, neuroprotective and osteoprotective actions. In Europe, medical mushrooms are mainly marketed in the form of food supplements as single components or combined with other nutraceuticals. In this context, the first peculiarity that distinguishes it is the safety established through the “history of consumption” that characterizes that mushroom. However, the cultivation of medicinal mushrooms on a large scale is performed mainly in China, where most of the production facilities do not have internationally recognized good manufacturing practices, despite that many European companies that sell myotherapies are supplied by Chinese manufacturers. This is particularly evident in Italy, where an arsenal of mushroom products is marketed in the form of powders and extracts not always of ascertained origin and sometimes of doubtful taxonomic identification, and thus not meeting the quality criteria required. The growing interest in mycotherapy involves a strong commitment from the scientific community to propose supplements of safe origin and genetic purity as well as to promote clinical trials to evaluate its real effects on humans. The purpose of this research is to analyze different mushroom-based dietary supplements used in medicine as monotherapy on the Italian market and to evaluate their composition and quality. The molecular identification of the sequences with those deposited in GenBank allowed for identifying 6 out of 19 samples, matching with those deposited belonging to the species indicated in the label, i.e., Lentinula edodes (samples 1, 4, 12 and 18) and Ganoderma lucidum (samples 5 and 10). Samples containing Ganoderma, labeled in the commercial product as G. lucidum, showed sequences that showed homology of 100% and 99% with G. resinaceum and G. sichuanense. An additional investigation was carried out in order to determine the active fungal ingredients, such as ergosterol, aflatoxins, heavy metals, nicotine and total glucan. The results obtained and shown in the manuscript highlight how the data were not only in line with what is expected with respect to what is indicated in the labels

Mushroom-Based Supplements in Italy: Let’s Open Pandora’s Box

Mushrooms and derivates are well known to the scientific community for having different health benefits and exhibit a wide range of pharmacological activities, including lipid-lowering, antihypertensive, antidiabetic, antimicrobic, antiallergic, anti-inflammatory, anticancer, immunomodulating, neuroprotective and osteoprotective actions. In Europe, medical mushrooms are mainly marketed in the form of food supplements as single components or combined with other nutraceuticals. In this context, the first peculiarity that distinguishes it is the safety established through the "history of consumption" that characterizes that mushroom. However, the cultivation of medicinal mushrooms on a large scale is performed mainly in China, where most of the production facilities do not have internationally recognized good manufacturing practices, despite that many European companies that sell myotherapies are supplied by Chinese manufacturers. This is particularly evident in Italy, where an arsenal of mushroom products is marketed in the form of powders and extracts not always of ascertained origin and sometimes of doubtful taxonomic identification, and thus not meeting the quality criteria required. The growing interest in mycotherapy involves a strong commitment from the scientific community to propose supplements of safe origin and genetic purity as well as to promote clinical trials to evaluate its real effects on humans. The purpose of this research is to analyze different mushroom-based dietary supplements used in medicine as monotherapy on the Italian market and to evaluate their composition and quality. The molecular identification of the sequences with those deposited in GenBank allowed for identifying 6 out of 19 samples, matching with those deposited belonging to the species indicated in the label, i.e., Lentinula edodes (samples 1, 4, 12 and 18) and Ganoderma lucidum (samples 5 and 10). Samples containing Ganoderma, labeled in the commercial product as G. lucidum, showed sequences that showed homology of 100% and 99% with G. resinaceum and G. sichuanense. An additional investigation was carried out in order to determine the active fungal ingredients, such as ergosterol, aflatoxins, heavy metals, nicotine and total glucan. The results obtained and shown in the manuscript highlight how the data were not only in line with what is expected with respect to what is indicated in the labels

Can anthocyanin presence ameliorate the photosynthetic performance of Prunus saplings subjected to polyethylene glycol-simulated water stress?

The aim was the evaluation of the biochemical and physiological responses of green- (GP) and red-leafed (RP) Prunus cerasifera mature leaves to 20 d of polyethylene glycol (PEG 6000)-induced water stress in order to elucidate a possible ameliorative role exerted by anthocyanins. At 10 d, the anthocyanin content remained unchanged in RP water-stressed leaves. Photosynthetic rate was lower in GP than that of RP (83.4 vs. 76.5%, respectively), paralleled by a higher degree of photoinhibition (Fv/Fm) in GP leaves. Leaves of GP accounted for higher content of soluble sugars at 10 d, when RP only showed a slight sucrose increase. At 20 d of stress, both morphs recovered their Fv/Fm values, suggesting the ability of both genotypes to adjust their photosynthetic metabolism under conditions of water stress. In conclusion, besides the sunscreen role served by anthocyanins, the carbon sink by these flavonoids might have further prevented sugar accumulation and the consequent sugar-promoted feedback regulation of photosynthesis in drought-stressed red leaves

Eficiência de inseticidas naturais e tiametoxam no controle de tripes em videira e seletividade para inimigos naturais.

O objetivo deste trabalho foi avaliar a eficiência de produtos naturais e do tiametoxam no controle de tripes em videira e seus efeitos sobre inimigos naturais. O experimento foi instalado em plantio comercial, no município de Juazeiro-BA (Brasil), em parreiral da variedade Benitaka, com 5 anos de idade, irrigado por microaspersão. Adotou-se o delineamento experimental em blocos ao acaso, com 4 repetições e 5 tratamentos, sendo: 1. Nim-I-Go a 0,5%; 2. Rotenat a 0,5%; 3. Bio Alho a 0,3%; 4. tiametoxam 250WG (20 g de produto comercial/100L) e 5. testemunha (sem inseticida). Foram utilizadas 12 plantas por tratamento, sendo avaliados o número de tripes e de inimigos naturais por inflorescência, em pré-aplicação e aos 2, 4 e 6 dias após a aplicação dos inseticidas. Os produtos naturais Rotenat e Nim-I-Go apresentaram as melhores porcentagens médias de controle (respectivamente, 52,04% e 48,37%), seguidos pelo tiametoxam (46,00%) e pelo Bio alho (23,07%). Aos dois dias após a aplicação, o tiametoxam apresentou eficiência de 76,79%, porém não manteve a eficiência ao longo das avaliações. As notas na escala de seletividade para o Bio Alho, Nim-I-Go, Rotenat e tiametoxam foram 2 (pouco tóxico), 3 (moderadamente tóxico), 3 e 4 (tóxico), respectivamente