Faria, Thiago S

Fink, Maria C D

Nali, Luiz H S

Olival, Guilherme S

Oliveira, Augusto C P

Romano, Camila M

Sumita, Laura M

Vidal, Jose E

English

PubMed

RCAAP - Repositório Científico de Acesso Aberto de Portugal

Lack of evidence of ERVWE1/Xq22.3 involvement in MRSV transcripts detected in Multiple Sclerosis patients

Romano, Camila M

Nali, Luiz H S

Faria, Thiago S

Olival, Guilherme S

Vidal, Jose E

Fink, Maria C D

Sumita, Laura M

Oliveira, Augusto C P

Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP)

  Universidade de São Paulo
 
2011
 
Lack of evidence of ERVWE1/Xq22.3
involvement in MRSV transcripts detected in
Multiple Sclerosis patients
 
 
Retrovirology. 2011 Jun 06;8(Suppl 1):A217
http://www.producao.usp.br/handle/BDPI/34834
 
Downloaded from: Biblioteca Digital da Produção Intelectual - BDPI, Universidade de São Paulo
Biblioteca Digital da Produção Intelectual - BDPI
Instituto de Medicina Tropical de São Paulo - IMT Artigos e Materiais de Revistas Científicas - IMT
MEETING ABSTRACT Open Access
Lack of evidence of ERVWE1/Xq22.3 involvement
in MRSV transcripts detected in Multiple Sclerosis
patients
Camila M Romano1*, Luiz H S Nali1, Thiago S Faria1, Guilherme S Olival2, Jose E Vidal3, Maria C D Fink1,
Laura M Sumita1, Augusto C P Oliveira3
From 15th International Conference on Human Retroviruses: HTLV and Related Viruses
Leuven and Gembloux, Belgium. 5-8 June 2011
HERV are viral ‘fossils’ that constitute 8% of the human
genome and have been implicated in both health and dis-
ease conditions. HERV-W/MRSV (Multiple Sclerosis
associated RetroVirus) RNA has been detected in patients
with multiple sclerosis. MS is a chronic inflammatory dis-
ease of the central nervous system of unknown cause,
resulting in demyelization and axonal degeneration.
MSRV is defined by different transcripts, which vary in
their source. Recently, it was described that most of
MSRV/Env transcripts from MS patients derived from
Xq22.3, 15q21.3 and 6q21. Although the genomic Xq22.3
MRSV/Env is truncated due to a stop codon (TGA) at
position 39, the transcripts associated harbored a trypto-
phan (TGG) instead. Thus, to evaluate if a polymorphism
at this position could be involved with full expression of
this locus in MS patients we sequenced 1085bp of the
ERVWE1/Xq22.3 from 15 MS patients of different ethnic
origins and different clinical presentations and 14 health
individuals. We found that all MS individuals harbor a
stop codon at position 39, undermining the expression of
a full-length ENV protein. No additional aminoacid sub-
stitution was found in both groups. Also, since there is
no LTR flanking this gene, we performed an automated
search for promoter sequences in 10kb nearby region.
We found several putative binding sites for cellular co-
factors and enhancers, suggesting that transcription may
occur via alternative promoters. Altogether, the findings
suggest that transcripts associated to ERVWE1/Xq22.3
previously detected may be artificially generated, possible
due to in vitro recombination between this locus and
other active ERVW/Env.
Author details
1Department of Infectious and Parasitic Diseases, Institute of Tropical
Medicine, School of Medicine, University of São Paulo, São Paulo,
SP, 05403-000, Brazil. 2Department of Neurology, Santa Casa de São Paulo,
Faculty of Medical Sciences, São Paulo, Brazil. 3HTLV Service, Institute of
Infectious Diseases Emillio Ribas, São Paulo,01246-000, Brazil.
Published: 6 June 2011
doi:10.1186/1742-4690-8-S1-A217
Cite this article as: Romano et al.: Lack of evidence of ERVWE1/Xq22.3
involvement in MRSV transcripts detected in Multiple Sclerosis patients.
Retrovirology 2011 8(Suppl 1):A217.
Submit your next manuscript to BioMed Central
and take full advantage of: 
• Convenient online submission
• Thorough peer review
• No space constraints or color figure charges
• Immediate publication on acceptance
• Inclusion in PubMed, CAS, Scopus and Google Scholar
• Research which is freely available for redistribution
Submit your manuscript at 
www.biomedcentral.com/submit
* Correspondence: cmromano@usp.br
1Department of Infectious and Parasitic Diseases, Institute of Tropical
Medicine, School of Medicine, University of São Paulo, São Paulo,
SP, 05403-000, Brazil
Full list of author information is available at the end of the article
Romano et al. Retrovirology 2011, 8(Suppl 1):A217
http://www.retrovirology.com/content/8/S1/A217
© 2011 Romano et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.


Camila M Romano

Luiz H S Nali

Thiago S Faria

Guilherme S Olival

Jose E Vidal

Maria C D Fink

Laura M Sumita

Augusto C P Oliveira

Springer - Publisher Connector

MEETING ABSTRACT Open Access
Lack of evidence of ERVWE1/Xq22.3 involvement
in MRSV transcripts detected in Multiple Sclerosis
patients
Camila M Romano1*, Luiz H S Nali1, Thiago S Faria1, Guilherme S Olival2, Jose E Vidal3, Maria C D Fink1,
Laura M Sumita1, Augusto C P Oliveira3
From 15th International Conference on Human Retroviruses: HTLV and Related Viruses
Leuven and Gembloux, Belgium. 5-8 June 2011
HERV are viral ‘fossils’ that constitute 8% of the human
genome and have been implicated in both health and dis-
ease conditions. HERV-W/MRSV (Multiple Sclerosis
associated RetroVirus) RNA has been detected in patients
with multiple sclerosis. MS is a chronic inflammatory dis-
ease of the central nervous system of unknown cause,
resulting in demyelization and axonal degeneration.
MSRV is defined by different transcripts, which vary in
their source. Recently, it was described that most of
MSRV/Env transcripts from MS patients derived from
Xq22.3, 15q21.3 and 6q21. Although the genomic Xq22.3
MRSV/Env is truncated due to a stop codon (TGA) at
position 39, the transcripts associated harbored a trypto-
phan (TGG) instead. Thus, to evaluate if a polymorphism
at this position could be involved with full expression of
this locus in MS patients we sequenced 1085bp of the
ERVWE1/Xq22.3 from 15 MS patients of different ethnic
origins and different clinical presentations and 14 health
individuals. We found that all MS individuals harbor a
stop codon at position 39, undermining the expression of
a full-length ENV protein. No additional aminoacid sub-
stitution was found in both groups. Also, since there is
no LTR flanking this gene, we performed an automated
search for promoter sequences in 10kb nearby region.
We found several putative binding sites for cellular co-
factors and enhancers, suggesting that transcription may
occur via alternative promoters. Altogether, the findings
suggest that transcripts associated to ERVWE1/Xq22.3
previously detected may be artificially generated, possible
due to in vitro recombination between this locus and
other active ERVW/Env.
Author details
1Department of Infectious and Parasitic Diseases, Institute of Tropical
Medicine, School of Medicine, University of São Paulo, São Paulo,
SP, 05403-000, Brazil. 2Department of Neurology, Santa Casa de São Paulo,
Faculty of Medical Sciences, São Paulo, Brazil. 3HTLV Service, Institute of
Infectious Diseases Emillio Ribas, São Paulo,01246-000, Brazil.
Published: 6 June 2011
doi:10.1186/1742-4690-8-S1-A217
Cite this article as: Romano et al.: Lack of evidence of ERVWE1/Xq22.3
involvement in MRSV transcripts detected in Multiple Sclerosis patients.
Retrovirology 2011 8(Suppl 1):A217.
Submit your next manuscript to BioMed Central
and take full advantage of: 
• Convenient online submission
• Thorough peer review
• No space constraints or color figure charges
• Immediate publication on acceptance
• Inclusion in PubMed, CAS, Scopus and Google Scholar
• Research which is freely available for redistribution
Submit your manuscript at 
www.biomedcentral.com/submit
* Correspondence: cmromano@usp.br
1Department of Infectious and Parasitic Diseases, Institute of Tropical
Medicine, School of Medicine, University of São Paulo, São Paulo,
SP, 05403-000, Brazil
Full list of author information is available at the end of the article
Romano et al. Retrovirology 2011, 8(Suppl 1):A217
http://www.retrovirology.com/content/8/S1/A217
© 2011 Romano et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.


Universidade de São Paulo

Crossref

MEETING ABSTRACT Open AccessLack of evidence of ERVWE1/Xq22.3 involvementin MRSV transcripts detected in Multiple SclerosispatientsCamila M Romano1*, Luiz H S Nali1, Thiago S Faria1, Guilherme S Olival2, Jose E Vidal3, Maria C D Fink1,Laura M Sumita1, Augusto C P Oliveira3From 15th International Conference on Human Retroviruses: HTLV and Related VirusesLeuven and Gembloux, Belgium. 5-8 June 2011HERV are viral ‘fossils’ that constitute 8% of the humangenome and have been implicated in both health and dis-ease conditions. HERV-W/MRSV (Multiple Sclerosisassociated RetroVirus) RNA has been detected in patientswith multiple sclerosis. MS is a chronic inflammatory dis-ease of the central nervous system of unknown cause,resulting in demyelization and axonal degeneration.MSRV is defined by different transcripts, which vary intheir source. Recently, it was described that most ofMSRV/Env transcripts from MS patients derived fromXq22.3, 15q21.3 and 6q21. Although the genomic Xq22.3MRSV/Env is truncated due to a stop codon (TGA) atposition 39, the transcripts associated harbored a trypto-phan (TGG) instead. Thus, to evaluate if a polymorphismat this position could be involved with full expression ofthis locus in MS patients we sequenced 1085bp of theERVWE1/Xq22.3 from 15 MS patients of different ethnicorigins and different clinical presentations and 14 healthindividuals. We found that all MS individuals harbor astop codon at position 39, undermining the expression ofa full-length ENV protein. No additional aminoacid sub-stitution was found in both groups. Also, since there isno LTR flanking this gene, we performed an automatedsearch for promoter sequences in 10kb nearby region.We found several putative binding sites for cellular co-factors and enhancers, suggesting that transcription mayoccur via alternative promoters. Altogether, the findingssuggest that transcripts associated to ERVWE1/Xq22.3previously detected may be artificially generated, possibledue to in vitro recombination between this locus andother active ERVW/Env.Author details1Department of Infectious and Parasitic Diseases, Institute of TropicalMedicine, School of Medicine, University of São Paulo, São Paulo,SP, 05403-000, Brazil. 2Department of Neurology, Santa Casa de São Paulo,Faculty of Medical Sciences, São Paulo, Brazil. 3HTLV Service, Institute ofInfectious Diseases Emillio Ribas, São Paulo,01246-000, Brazil.Published: 6 June 2011doi:10.1186/1742-4690-8-S1-A217Cite this article as: Romano et al.: Lack of evidence of ERVWE1/Xq22.3involvement in MRSV transcripts detected in Multiple Sclerosis patients.Retrovirology 2011 8(Suppl 1):A217.Submit your next manuscript to BioMed Centraland take full advantage of: • Convenient online submission• Thorough peer review• No space constraints or color figure charges• Immediate publication on acceptance• Inclusion in PubMed, CAS, Scopus and Google Scholar• Research which is freely available for redistributionSubmit your manuscript at www.biomedcentral.com/submit* Correspondence: cmromano@usp.br1Department of Infectious and Parasitic Diseases, Institute of TropicalMedicine, School of Medicine, University of São Paulo, São Paulo,SP, 05403-000, BrazilFull list of author information is available at the end of the articleRomano et al. Retrovirology 2011, 8(Suppl 1):A217http://www.retrovirology.com/content/8/S1/A217© 2011 Romano et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative CommonsAttribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited.

Retrovirology

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3112695

Lack of evidence of ERVWE1/Xq22.3 involvement in MRSV transcripts detected in Multiple Sclerosis patients

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Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP)

Springer - Publisher Connector

Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP)

Universidade de São Paulo

Universidade de São Paulo

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