2,767 research outputs found
Substrate-specific clades of active marine methylotrophs associated with a phytoplankton bloom in a temperate coastal environment
Marine microorganisms that consume one-carbon (C1) compounds are poorly described, despite their impact on global climate via an influence on aquatic and atmospheric chemistry. This study investigated marine bacterial communities involved in the metabolism of C1 compounds. These communities were of relevance to surface seawater and atmospheric chemistry in the context of a bloom that was dominated by phytoplankton known to produce dimethylsulfoniopropionate. In addition to using 16S rRNA gene fingerprinting and clone libraries to characterize samples taken from a bloom transect in July 2006, seawater samples from the phytoplankton bloom were incubated with 13C-labeled methanol, monomethylamine, dimethylamine, methyl bromide, and dimethyl sulfide to identify microbial populations involved in the turnover of C1 compounds, using DNA stable isotope probing. The [13C]DNA samples from a single time point were characterized and compared using denaturing gradient gel electrophoresis (DGGE), fingerprint cluster analysis, and 16S rRNA gene clone library analysis. Bacterial community DGGE fingerprints from 13C-labeled DNA were distinct from those obtained with the DNA of the nonlabeled community DNA and suggested some overlap in substrate utilization between active methylotroph populations growing on different C1 substrates. Active methylotrophs were affiliated with Methylophaga spp. and several clades of undescribed Gammaproteobacteria that utilized methanol, methylamines (both monomethylamine and dimethylamine), and dimethyl sulfide. rRNA gene sequences corresponding to populations assimilating 13C-labeled methyl bromide and other substrates were associated with members of the Alphaproteobacteria (e.g., the family Rhodobacteraceae), the Cytophaga-Flexibacter-Bacteroides group, and unknown taxa. This study expands the known diversity of marine methylotrophs in surface seawater and provides a comprehensive data set for focused cultivation and metagenomic analyses in the future
LaSER oceanography: Data report number 1, R/V Pelican cruise, July 21-August 1, 1987, CTD and hydrographic data
The LaSER oceanography program is a five year multi-institutional and multi-investigator program titled "Oceanographic Processes on Continental Shelves Influenced by Large Rivers." Funding for this program began in January, 1987.
The scientific goals of this program are: a) investigations on a large spatial scale, from the Mississippi River delta to some far field (down-plume) location, to examine biological responses to riverine inputs of dissolved nutrients, suspended sediments, and fresh water; b) investigations on small spatial scales, both horizontally and vertically, in a cross plume direction to examine the role of oceanographic fronts, convergences, and discontinuities in biological production; and c) investigations on small temporal scales, particularly to examine the biological responses to the passage of winter storms.
This report summarizes the CTD and hydrographic (bottle) data from the first LaSER oceanography cruise
Comparison of bacterioneuston and bacterioplankton dynamics during a phytoplankton bloom in a fjord mesocosm
The bacterioneuston is the community of Bacteria present in surface microlayers, the
thin surface film that forms the interface between aquatic environments and the
atmosphere. In this study we compared bacterial cell abundance and bacterial
community structure of the bacterioneuston and the bacterioplankton (from the
subsurface water column) during a phytoplankton bloom mesocosm experiment.
Bacterial cell abundance, determined by flow cytometry, followed a typical
bacterioplankton response to a phytoplankton bloom, with Synechococcus and high
nucleic acid (HNA) bacterial cell numbers initially falling, probably due to selective
protist grazing. Subsequently HNA and low nucleic acid (LNA) bacterial cells
increased in abundance but Synechococcus did not. There was no significant
difference between bacterioneuston and bacterioplankton cell abundances during the
experiment. Conversely, distinct and consistent differences between the
bacterioneuston and the bacterioplankton community structure were observed. This
was monitored simultaneously by Bacteria 16S rRNA gene terminal restriction
fragment length polymorphism (T-RFLP) and denaturing gradient gel electrophoresis
(DGGE). The conserved patterns of community structure observed in all of the
mesocosms indicate that the bacterioneuston is distinctive and non-random
High Cyanobacterial Abundance in Three Northeastern Gulf of Mexico Estuaries
Aquatic phytoplankton comprise a wide variety of taxa spanning more than 2 orders of magnitude in size, yet studies of estuarine phytoplankton often overlook the picoplankton, particularly chroococcoid cyanobacteria (cf. Synechococcus). Three Gulf of Mexico estuaries (Apalachicola Bay, FL; Pensacola Bay, FL; Weeks Bay, AL) were sampled during summer and fall 2001 to quantify cyanobacterial abundance, to examine how cyanobacterial abundance varied with hydrographic and nutrient distributions, and to estimate the contribution of cyanobacteria to the bulk phytoplankton community. Cyanobacterial abundances in all 3 estuaries were high, averaging 0.59 ± 0.76 X 109 L–1 in Apalachicola Bay, 1.7 ± 1.2 X 109 L–1 in Pensacola Bay and 2.4 ± 1.9 X 109 L–1 in Weeks Bay (mean ± standard deviation). Peak abundances typically occurred in the oligohaline zone (low salinity estuarine zone) during the summer. Freshwater sites had nearly undetectable abundances, and marine sites had abundances several-fold lower than the oligohaline zone. When converted to equivalent chlorophyll a concentrations, cyanobacteria comprised a large fraction of the total phytoplankton biomass, at times approaching 100% in all 3 systems. These observations clearly indicate a cyanobacterial community of estuarine origin that can make up a large proportion of phytoplankton biomass
Alpha- and Gammaproteobacterial Methanotrophs Codominate the Active Methane-Oxidizing Communities in an Acidic Boreal Peat Bog
The objective of this study was to characterize metabolically active, aerobic methanotrophs in an ombrotrophic peatland in the Marcell Experimental Forest, Minnesota, USA. Methanotrophs were investigated in the field and in laboratory incubations using DNA-stable isotope probing, expression studies on particulate methane monooxygenase (pmoA) genes, and amplicon sequencing of 16S rRNA genes. Potential rates of oxidation ranged from 14-17 μmol CH4 g dry wt soil-1 d-1. Within DNA-SIP incubations, the relative abundance of methanotrophs increased from 4% in situ to 25-36% after 8 -14 days. Phylogenetic analysis of the 13C-enriched DNA fractions revealed the active methanotrophs were dominated by the genera Methylocystis (Type II; Alphaproteobacteria), Methylomonas, and Methylovulum (Type I; Gammaproteobacteria). In field samples, a transcript-to-gene ratio of 1 to 2 was observed for pmoA in surface peat layers which attenuated rapidly with depth, indicating the highest methane consumption was associated with the 0-10 cm depth interval. Metagenomes and sequencing of cDNA pmoA amplicons from field samples confirmed the dominant active methanotrophs were Methylocystis and Methylomonas. Although Type II methanotrophs have long been shown to mediate methane consumption in peatlands, our results indicate members of the genera Methylomonas and Methylovulum (Type I) can significantly contribute to aerobic methane oxidation in these ecosystems
Multivariate geometric anisotropic Cox processes
This paper introduces a new modeling and inference framework for multivariate and anisotropic point processes. Building on recent innovations in multivariate spatial statistics, we propose a new family of multivariate anisotropic random fields, and from them a family of anisotropic point processes. We give conditions that make the proposed models valid. We also propose a Palm likelihood-based inference method for this type of point process, circumventing issues of likelihood tractability. Finally we illustrate the utility of the proposed modeling framework by analyzing spatial ecological observations of plants and trees in the Barro Colorado Island data
IL-21 receptor expression in human tendinopathy
The pathogenetic mechanisms underlying tendinopathy remain unclear,
with much debate as to whether inflammation or degradation has the prominent
role. Increasing evidence points toward and early inflammatory infiltrate and
associated inflammatory cytokine production in human and animal models of
tendon disease.
The IL-21/IL-21R axis is a proinflammatory cytokine complex that has
been associated with chronic inflammatory diseases including rheumatoid
arthritis and inflammatory bowel disease. This project aimed to investigate the
role and expression of the cytokine/receptor pair IL-21/IL-21R in human
tendinopathy.
We found significantly elevated expression of IL-21 receptor message and
protein in human tendon samples but found no convincing evidence of the
presence of IL-21 at message or protein level. The level of expression of IL-21R
message/protein in human tenocytes was significantly up regulated by
proinflammatory cytokines (TNFα/IL-1β) in vitro.
These findings demonstrate that IL-21R is present in early human
tendinopathy mainly expressed by tenocytes and macrophages. Despite a lack of
IL-21 expression these data again suggest that early tendinopathy has an
inflammatory/cytokine phenotype, which may provide novel translational targets
in the treatment of tendinopathy
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