Realidad jurídico-laboral en el aula. Guías de trabajo autónomo con medios audiovisuales

This article wants to show an innovative project in the teaching of legal subjects in their side of formal proceedings, so the students could practise using their knowledge of the substantive law, a completed diagnosis of a case, and be able to provide adequate legal guidance to the legal practice. The objective is inter relation they theoretic knowledge that they have learned in the classroom with their effective implementation at the practical reality, pursuing convergence with the European Higher Education Area. Help to promote self-employment and student self-learning, including the strengthening and updating the necessary innovation in teaching and learning methodologies, using new information technologies and communication applied to teaching, consisting of actual recordings of subjects in relation to the course that we have begun to apply the labour process.Este artículo pretende mostrar un proyecto innovador en la enseñanza de asignaturas jurídicas, en su vertiente procesal o de carácter formal, para que el alumno pueda realizar, una vez que conoce el derecho sustantivo, un completo diagnóstico de un caso concreto, y ser capaz de dar la orientación jurídica adecuada a la práctica jurídica. El objetivo es que interrelacionen los conocimientos teóricos que van adquiriendo en el aula con su aplicación efectiva a la realidad práctica, persiguiéndose la convergencia con el Espacio Europeo de Educación Superior. Contribuye a la promoción del trabajo autónomo y autoaprendizaje del alumno, fortaleciendo además la necesaria innovación y actualización en metodologías de enseñanza-aprendizaje, utilizando las nuevas tecnologías de la información y la comunicación aplicadas a la docencia, consistentes en grabaciones reales de materias en relación a la asignatura a la que lo hemos empezado a aplicar, el Proceso Laboral

SEROPREVALENCIA DE Neospora caninum EN LLAMAS DE LA PROVINCIA DE MELGAR, PUNO

La neoporosis, causada por el protozoo Neospora caninum, ha sido asociada con problemas reproductivos en la ganadería bovina a nivel mundial, comprobándose que este parásito puede infectar a diversas especies, entre ellos los camellos. El objetivo del presente trabajo fue determinar la seroprevalencia de Neospora caninum en dos centros de crianza de llamas de la provincia de Melgar, Puno. Se evaluaron 275 sueros de llamas hembras en los distritos de Santa Rosa (n = 86) y de Nuñoa (n = 189), mediante la prueba de inmunofluorescencia indirecta. El 16.7 ± 4.4 % (46/275) presentó anticuerpos contra el parásito a una dilución de 1:50, considerándose como una seroprevalencia moderada. El fundo de Santa Rosa presentó una seroprevalencia baja (4.7 ± 4.5%, 4/86), mientras que en el de Nuñoa fue moderada (22.2 ± 5.9 %, 42/189), existiendo diferencias estadísticas significativas entre ambos fundos (p<0.05). No se encontraron diferencias significativas entre grupos etáreos (p>0.05). Este estudio confirma la presencia de Neospora caninum en llamas hembras de la provincia de Melgar, Puno.Neosporosis, caused by the protozoan Neospora caninum, has been associated with reproductive problems in cattle worldwide, and has been found in a variety of animal species, including camels. The aim of this work was to determine the seroprevalence of Neospora caninum in two llama herds of Melgar province, Puno. Two hundred seventy five serum samples of female llamas from the districts of Santa Rosa and Nuñoa were tested using the indirect inmunofluorescense test (IFAT) to detect antibodies against N. caninum. The 16.7 ± 4.4% (46/275) of the serum samples had antibodies at 1:50 dilution, indicating a moderate seroprevalence in the herds. The seroprevalence in Santa Rosa was lower (4.6 ± 4.4%, 4/86) than in Nuñoa (22.2 ± 5.9%, 42/189), and with statistical differences between herds (p<0.05). No differences due to age were found (p>0.05). This study confirms the presence of Neospora caninum in female llamas from Melgar province, Puno

EPA, DHA, and Lipoic Acid Differentially Modulate the n-3 Fatty Acid Biosynthetic Pathway in Atlantic Salmon Hepatocytes

The aim of the present study was to investigate how EPA, DHA, and lipoic acid (LA) influence the different metabolic steps in the n-3 fatty acid (FA) biosynthetic pathway in hepatocytes from Atlantic salmon fed four dietary levels (0, 0.5, 1.0 and 2.0%) of EPA, DHA or a 1:1 mixture of these FA. The hepatocytes were incubated with [1-14C] 18:3n-3 in the presence or absence of LA (0.2 mM). Increased endogenous levels of EPA and/or DHA and LA exposure both led to similar responses in cells with reduced desaturation and elongation of [1-14C] 18:3n-3 to 18:4n-3, 20:4n-3, and EPA, in agreement with reduced expression of the Δ6 desaturase gene involved in the first step of conversion. DHA production, on the other hand, was maintained even in groups with high endogenous levels of DHA, possibly due to a more complex regulation of this last step in the n-3 metabolic pathway. Inhibition of the Δ6 desaturase pathway led to increased direct elongation to 20:3n-3 by both DHA and LA. Possibly the route by 20:3n-3 and then Δ8 desaturation to 20:4n-3, bypassing the first Δ6 desaturase step, can partly explain the maintained or even increased levels of DHA production. LA increased DHA production in the phospholipid fraction of hepatocytes isolated from fish fed 0 and 0.5% EPA and/or DHA, indicating that LA has the potential to further increase the production of this health-beneficial FA in fish fed diets with low levels of EPA and/or DHA.submittedVersio