1,267 research outputs found

    Applications of satellite snow cover in computerized short-term streamflow forecasting

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    A procedure is described whereby the correlation between: (1) satellite derived snow-cover depletion and (2) residual snowpack water equivalent, can be used to update computerized residual flow forecasts for the Conejos River in southern Colorado

    LANDSAT derived snowcover as an input variable for snowmelt runoff forecasting in south central Colorado

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    There are no author-identified significant results in this report

    Snowpack ground truth: Radar test site, Steamboat Springs, Colorado, 8-16 April 1976

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    Ground-truth data taken at Steamboat Springs, Colorado is presented. Data taken during the period April 8, 1976 - April 16, 1976 included the following: (1) snow depths and densities at selected locations (using a Mount Rose snow tube); (2) snow pits for temperature, density, and liquid water determinations using the freezing calorimetry technique and vertical layer classification; (3) snow walls were also constructed of various cross sections and documented with respect to sizes and snow characteristics; (4) soil moisture at selected locations; and (5) appropriate air temperature and weather data

    A dynamically adaptive multigrid algorithm for the incompressible Navier-Stokes equations: Validation and model problems

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    An algorithm is described for the solution of the laminar, incompressible Navier-Stokes equations. The basic algorithm is a multigrid based on a robust, box-based smoothing step. Its most important feature is the incorporation of automatic, dynamic mesh refinement. This algorithm supports generalized simple domains. The program is based on a standard staggered-grid formulation of the Navier-Stokes equations for robustness and efficiency. Special grid transfer operators were introduced at grid interfaces in the multigrid algorithm to ensure discrete mass conservation. Results are presented for three models: the driven-cavity, a backward-facing step, and a sudden expansion/contraction

    Applications systems verification and transfer project. Volume 4: Operational applications of satellite snow cover observations. Colorado Field Test Center

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    The study was conducted on six watersheds ranging in size from 277 km to 3460 km in the Rio Grande and Arkansas River basins of southwestern Colorado. Six years of satellite data in the period 1973-78 were analyzed and snowcover maps prepared for all available image dates. Seven snowmapping techniques were explored; the photointerpretative method was selected as the most accurate. Three schemes to forecast snowmelt runoff employing satellite snowcover observations were investigated. They included a conceptual hydrologic model, a statistical model, and a graphical method. A reduction of 10% in the current average forecast error is estimated when snowcover data in snowmelt runoff forecasting is shown to be extremely promising. Inability to obtain repetitive coverage due to the 18 day cycle of LANDSAT, the occurrence of cloud cover and slow image delivery are obstacles to the immediate implementation of satellite derived snowcover in operational streamflow forecasting programs

    Quantification of cellular protein expression and molecular features of group 3 LEA proteins from embryos of Artemia franciscana

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    Late embryogenesis abundant (LEA) proteins are highly hydrophilic, low complexity proteins whose expression has been correlated with desiccation tolerance in anhydrobiotic organisms. Here, we report the identification of three new mitochondrial LEA proteins in anhydrobiotic embryos of Artemia franciscana, AfrLEA3m-47, AfrLEA3m-43, and AfrLEA3m-29. These new isoforms are recognized by antibody raised against recombinant AfrLEA3m, the original mitochondrial-targeted LEA protein previously reported from these embryos; mass spectrometry confirms all four proteins share sequence similarity. The corresponding messenger RNA (mRNA) species for the four proteins are readily amplified from total complementary DNA (cDNA) prepared from embryos. cDNA sequences of the four mRNAs are quite similar, but each has a stretch of sequence that is absent in at least one of the others, plus multiple single base pair differences. We conclude that all four mitochondrial LEA proteins are products of independent genes. Each possesses a mitochondrial targeting sequence, and indeed Western blots performed on extracts of isolated mitochondria clearly detect all four isoforms. Based on mass spectrometry and sodium dodecyl sulfate polyacrylamide gel electrophoresis migration, the cytoplasmic-localized AfrLEA2 exists primarily as a homodimer in A. franciscana. Quantification of protein expression for AfrLEA2, AfrLEA3m, AfrLEA3m-43, and AfrLEA3m-29 as a function of development shows that cellular concentrations are highest in diapause embryos and decrease during development to low levels in desiccation-intolerant nauplius larvae. When adjustment is made for mitochondria matrix volume, the effective concentrations of cytoplasmic versus mitochondrial group 3 LEA proteins are similar in vivo, and the values provide guidance for the design of in vitro functional studies with these proteins. © 2013 Cell Stress Society International

    Group 3 late embryogenesis abundant proteins from embryos of Artemia franciscana : structural properties and protective abilities during desiccation.

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    Group 3 late embryogenesis abundant (LEA) proteins are highly hydrophilic, and their expression is associated with desiccation tolerance in both plants and animals. Here we show that two LEA proteins from embryos of Artemia franciscana, AfrLEA2 and AfrLEA3m, are intrinsically disordered in solution but upon desiccation gain secondary structure, as measured by circular dichroism. Trifluoroethanol and sodium dodecyl sulfate are both shown to induce a-helical structure in AfrLEA2 and AfrLEA3m. Bioinformatic predictions of secondary-structure content for both proteins correspond most closely to conformations measured in the dry state. Because some LEA proteins afford protection to desiccation-sensitive proteins during drying and subsequent rehydration, we tested for this capacity in AfrLEA2 and AfrLEA3m. The protective capacities vary, depending on the target enzyme. For the cytoplasmic enzyme lactate dehydrogenase, neither AfrLEA2 nor AfrLEA3m, with or without trehalose present, was able to afford protection better than that provided by bovine serum albumin (BSA) under the same conditions. However, for another cytoplasmic enzyme, phosphofructokinase, both AfrLEA2 and AfrLEA3m in the presence of trehalose were able to afford protection far greater than that provided by BSA with trehalose. Finally, for the mitochondrial enzyme citrate synthase, 400-mg/mL AfrLEA3m without trehalose provided significantly more protection than the same concentration of either AfrLEA2 or BSA

    Ethnicity, educational attainment, and physical health of older adults in the United States

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    ObjectiveMinorities’ diminished returns theory suggests that socioeconomic status (SES) resources generate fewer health benefits for racial and ethnic minority groups, compared to the majority group. The current study aimed to compare Hispanic and non‐Hispanic white older adults for the association between educational attainment and poor physical self‐rated health (SRH).MethodsThe first wave of the University of Michigan National Poll on Healthy Aging (UM‐NPHA) included 1820 older adults who were 50‐80 years old and were either non‐Hispanic white (n = 1618) or Hispanic white (n = 202). The main independent variable of interest was educational attainment. The main dependent variable of interest was poor physical SRH. Gender, age, marital status, and employment status were covariates. Ethnicity was the focal effect modifier.ResultsOverall, higher level of educational attainment was associated with better physical SRH. A significant interaction was found between ethnicity and level of educational attainment, which was indicative of a smaller physical SRH gain due to high educational attainment for Hispanic white compared to non‐Hispanic white older adults. In ethnic‐specific models, we found evidence suggesting that high educational attainment reduced the odds of poor physical SRH for non‐Hispanic whites but not for Hispanic whites.ConclusionCompared to non‐Hispanic whites, Hispanic whites gain less physical SRH benefits from their educational attainment.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/149760/1/agm212050_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/149760/2/agm212050.pd

    Orthogonal, solenoidal, three-dimensional vector fields for no-slip boundary conditions

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    Viscous fluid dynamical calculations require no-slip boundary conditions. Numerical calculations of turbulence, as well as theoretical turbulence closure techniques, often depend upon a spectral decomposition of the flow fields. However, such calculations have been limited to two-dimensional situations. Here we present a method that yields orthogonal decompositions of incompressible, three-dimensional flow fields and apply it to periodic cylindrical and spherical no-slip boundaries.Comment: 16 pages, 2 three-part figure

    Application of the collocation method using hermite cubic splines to nonlinear transient one-dimensional heat-conduction problems

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    This report addresses the application of the collocation method using hermite cubic splines to nonlinear transient one-dimensional heat conduction problems
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